(Linear) (Six-base) MAPSORT of: KIBB7683.seq Check: 4190 from: 1 to: 1038 KIBB7683.orf With 182 enzymes: SgfI * June 26, 2008 10:49 .. AatII G_ACGT'C Cuts at: 0 233 1038 Size: 233 805 AccI GT'mk_AC Cuts at: 0 917 1038 Size: 917 121 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 827 1038 Size: 827 211 AfeI AGC'GCT Cuts at: 0 817 1038 Size: 817 221 AlwNI CAG_nnn'CTG Cuts at: 0 304 1038 Size: 304 734 ApaI G_GGCC'C Cuts at: 0 190 1038 Size: 190 848 ApaLI G'TGCA_C Cuts at: 0 682 1038 Size: 682 356 ApoI r'AATT_y Cuts at: 0 105 1038 Size: 105 933 BanII G_rGCy'C Cuts at: 0 190 791 1038 Size: 190 601 247 Fragments arranged by size: 601 247 190 BbsI GAAGACnn'nnnn_ Cuts at: 0 944 1038 Size: 944 94 BglII A'GATC_T Cuts at: 0 211 1038 Size: 211 827 Bme1580I G_kGCm'C Cuts at: 0 190 686 1038 Size: 190 496 352 Fragments arranged by size: 496 352 190 BmgBI CAC'GTC Cuts at: 0 672 1038 Size: 672 366 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 991 1038 Size: 991 47 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 961 1038 Size: 141 820 77 Fragments arranged by size: 820 141 77 BsaHI Gr'CG_yC Cuts at: 0 230 1038 Size: 230 808 BsaWI w'CCGG_w Cuts at: 0 10 1038 Size: 10 1028 BsiHKAI G_wGCw'C Cuts at: 0 686 1038 Size: 686 352 BsmBI CGTCTCn'nnnn_ Cuts at: 0 37 1038 Size: 37 1001 Bsp1286I G_dGCh'C Cuts at: 0 190 686 791 1038 Size: 190 496 105 247 Fragments arranged by size: 496 247 190 105 BspEI T'CCGG_A Cuts at: 0 10 1038 Size: 10 1028 BsrDI GCAATG_nn' Cuts at: 0 618 1038 Size: 618 420 BsrGI T'GTAC_A Cuts at: 0 405 1038 Size: 405 633 BstYI r'GATC_y Cuts at: 0 211 1038 Size: 211 827 BstZ17I GTA'TAC Cuts at: 0 918 1038 Size: 918 120 EarI CTCTTCn'nnn_ Cuts at: 0 872 1004 1038 Size: 872 132 34 Fragments arranged by size: 872 132 34 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 827 1038 Size: 827 211 EcoNI CCTnn'n_nnAGG Cuts at: 0 194 1038 Size: 194 844 EcoO109I rG'GnC_Cy Cuts at: 0 187 1038 Size: 187 851 HaeII r_GCGC'y Cuts at: 0 819 1038 Size: 819 219 HindIII A'AGCT_T Cuts at: 0 644 1038 Size: 644 394 MslI CAynn'nnrTG Cuts at: 0 329 1038 Size: 329 709 PasI CC'CwG_GG Cuts at: 0 190 383 1038 Size: 190 193 655 Fragments arranged by size: 655 193 190 PfoI T'CCnGG_A Cuts at: 0 113 273 636 1038 Size: 113 160 363 402 Fragments arranged by size: 402 363 160 113 PspOMI G'GGCC_C Cuts at: 0 186 1038 Size: 186 852 SfcI C'TryA_G Cuts at: 0 477 1038 Size: 477 561 SmlI C'TyrA_G Cuts at: 0 120 976 1038 Size: 120 856 62 Fragments arranged by size: 856 120 62 SspI AAT'ATT Cuts at: 0 471 1038 Size: 471 567 TatI w'GTAC_w Cuts at: 0 405 1038 Size: 405 633 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 92 124 1038 Size: 92 32 914 Fragments arranged by size: 914 92 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 663 1038 Size: 663 375 ZraI GAC'GTC Cuts at: 0 231 1038 Size: 231 807 Enzymes that do cut: AatII AccI AcuI AfeI AlwNI ApaI ApaLI ApoI BanII BbsI BglII Bme1580I BmgBI BplI BpuEI BsaHI BsaWI BsiHKAI BsmBI Bsp1286I BspEI BsrDI BsrGI BstYI BstZ17I EarI Eco57MI EcoNI EcoO109I HaeII HindIII MslI PasI PfoI PspOMI SfcI SmlI SspI TatI TstI XcmI ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI