Restriction map for
pF1KB7688
(Linear) (Six-base) MAPSORT of: KIBB7688.seq Check: 8992 from: 1 to: 1230
KIBB7688.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 782 1230
Size: 782 448
AccI GT'mk_AC
Cuts at: 0 506 1230
Size: 506 724
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1230
Size: 919 311
AhdI GACnn_n'nnGTC
Cuts at: 0 502 1230
Size: 502 728
AleI CACnn'nnGTG
Cuts at: 0 226 1230
Size: 226 1004
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 529 561 1230
Size: 265 32 232 32 669
Fragments arranged by size:
669 265 232 32 32
ApoI r'AATT_y
Cuts at: 0 838 1230
Size: 838 392
AvaI C'yCGr_G
Cuts at: 0 70 124 1230
Size: 70 54 1106
Fragments arranged by size:
1106 70 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 39 811 956 1230
Size: 39 772 145 274
Fragments arranged by size:
772 274 145 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 409 443 1230
Size: 409 34 787
Fragments arranged by size:
787 409 34
BmgBI CAC'GTC
Cuts at: 0 1058 1230
Size: 1058 172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 1009 1134 1230
Size: 946 63 125 96
Fragments arranged by size:
946 125 96 63
Bpu10I CC'TnA_GC
Cuts at: 0 596 1013 1230
Size: 596 417 217
Fragments arranged by size:
596 417 217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 1230
Size: 25 1205
BsaI GGTCTCn'nnnn_
Cuts at: 0 198 1139 1230
Size: 198 941 91
Fragments arranged by size:
941 198 91
BsaAI yAC'GTr
Cuts at: 0 999 1230
Size: 999 231
BsaWI w'CCGG_w
Cuts at: 0 1164 1230
Size: 1164 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 529 553 559 583 1132 1162 1230
Size: 529 24 6 24 549 30 68
Fragments arranged by size:
549 529 68 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 279 645 1207 1230
Size: 279 366 562 23
Fragments arranged by size:
562 366 279 23
BseYI C'CCAG_C
Cuts at: 0 176 250 1230
Size: 176 74 980
Fragments arranged by size:
980 176 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 730 1121 1230
Size: 400 330 391 109
Fragments arranged by size:
400 391 330 109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 782 1039 1230
Size: 782 257 191
Fragments arranged by size:
782 257 191
BsrBI CCG'CTC
Cuts at: 0 123 1230
Size: 123 1107
BsrDI GCAATG_nn'
Cuts at: 0 170 1230
Size: 170 1060
BsrFI r'CCGG_y
Cuts at: 0 13 207 719 743 1230
Size: 13 194 512 24 487
Fragments arranged by size:
512 487 194 24 13
BssSI C'ACGA_G
Cuts at: 0 91 1230
Size: 91 1139
BstAPI GCAn_nnn'nTGC
Cuts at: 0 10 138 917 1230
Size: 10 128 779 313
Fragments arranged by size:
779 313 128 10
BstXI CCAn_nnnn'nTGG
Cuts at: 0 972 987 1230
Size: 972 15 243
Fragments arranged by size:
972 243 15
DrdI GACnn_nn'nnGTC
Cuts at: 0 1167 1230
Size: 1167 63
EaeI y'GGCC_r
Cuts at: 0 95 530 745 962 1115 1230
Size: 95 435 215 217 153 115
Fragments arranged by size:
435 217 215 153 115 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 946 1009 1134 1230
Size: 919 27 63 125 96
Fragments arranged by size:
919 125 96 63 27
EcoO109I rG'GnC_Cy
Cuts at: 0 342 1230
Size: 342 888
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 153 1230
Size: 153 1077
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 488 520 1230
Size: 488 32 710
Fragments arranged by size:
710 488 32
HincII GTy'rAC
Cuts at: 0 117 168 792 1230
Size: 117 51 624 438
Fragments arranged by size:
624 438 117 51
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 1005 1230
Size: 308 697 225
Fragments arranged by size:
697 308 225
MscI TGG'CCA
Cuts at: 0 532 964 1117 1230
Size: 532 432 153 113
Fragments arranged by size:
532 432 153 113
MslI CAynn'nnrTG
Cuts at: 0 226 892 1230
Size: 226 666 338
Fragments arranged by size:
666 338 226
MspA1I CmG'CkG
Cuts at: 0 1145 1230
Size: 1145 85
NaeI GCC'GGC
Cuts at: 0 745 1230
Size: 745 485
NgoMIV G'CCGG_C
Cuts at: 0 743 1230
Size: 743 487
NspI r_CATG'y
Cuts at: 0 727 1230
Size: 727 503
PflMI CCAn_nnn'nTGG
Cuts at: 0 183 1230
Size: 183 1047
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 1230
Size: 529 32 669
Fragments arranged by size:
669 529 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 665 697 1230
Size: 665 32 533
Fragments arranged by size:
665 533 32
PstI C_TGCA'G
Cuts at: 0 823 1230
Size: 823 407
SexAI A'CCwGG_T
Cuts at: 0 704 1230
Size: 704 526
SfcI C'TryA_G
Cuts at: 0 682 819 1230
Size: 682 137 411
Fragments arranged by size:
682 411 137
SmaI CCC'GGG
Cuts at: 0 72 1230
Size: 72 1158
SmlI C'TyrA_G
Cuts at: 0 40 1230
Size: 40 1190
SphI G_CATG'C
Cuts at: 0 727 1230
Size: 727 503
StyI C'CwwG_G
Cuts at: 0 295 548 980 1230
Size: 295 253 432 250
Fragments arranged by size:
432 295 253 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 771 1230
Size: 771 459
TatI w'GTAC_w
Cuts at: 0 1139 1230
Size: 1139 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 553 585 1230
Size: 553 32 645
Fragments arranged by size:
645 553 32
XmaI C'CCGG_G
Cuts at: 0 70 1230
Size: 70 1160
XmnI GAAnn'nnTTC
Cuts at: 0 677 1230
Size: 677 553
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AloI ApoI AvaI
BbsI BcgI BmgBI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstXI DrdI EaeI Eco57MI EcoO109I
FalI Hin4I HincII MmeI MscI MslI MspA1I NaeI
NgoMIV NspI PflMI PpiI PsrI PstI SexAI SfcI
SmaI SmlI SphI StyI TaqII TatI TstI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AlfI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BsaBI
BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI ZraI