Restriction map for
pF1KB7689
(Linear) (Six-base) MAPSORT of: KIBB7689.seq Check: 6623 from: 1 to: 1047
KIBB7689.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 0 323 1047
Size: 323 724
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 1047
Size: 300 747
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 910 1047
Size: 910 137
AlwNI CAG_nnn'CTG
Cuts at: 0 911 1047
Size: 911 136
ApoI r'AATT_y
Cuts at: 0 237 255 1047
Size: 237 18 792
Fragments arranged by size:
792 237 18
AvaI C'yCGr_G
Cuts at: 0 63 803 989 1047
Size: 63 740 186 58
Fragments arranged by size:
740 186 63 58
BanI G'GyrC_C
Cuts at: 0 894 1047
Size: 894 153
BanII G_rGCy'C
Cuts at: 0 71 656 1047
Size: 71 585 391
Fragments arranged by size:
585 391 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 395 674 1047
Size: 395 279 373
Fragments arranged by size:
395 373 279
BbvCI CC'TCA_GC
Cuts at: 0 787 1047
Size: 787 260
BfrBI ATG'CAT
Cuts at: 0 117 1047
Size: 117 930
BglI GCCn_nnn'nGGC
Cuts at: 0 753 1047
Size: 753 294
BglII A'GATC_T
Cuts at: 0 668 1047
Size: 668 379
Bme1580I G_kGCm'C
Cuts at: 0 719 1047
Size: 719 328
BmrI ACTGGGnnnn_n'
Cuts at: 0 180 1047
Size: 180 867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 847 1047
Size: 55 792 200
Fragments arranged by size:
792 200 55
Bpu10I CC'TnA_GC
Cuts at: 0 787 1047
Size: 787 260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 1047
Size: 55 992
BsaI GGTCTCn'nnnn_
Cuts at: 0 979 1047
Size: 979 68
BsaHI Gr'CG_yC
Cuts at: 0 832 1047
Size: 832 215
BsaWI w'CCGG_w
Cuts at: 0 855 891 1047
Size: 855 36 156
Fragments arranged by size:
855 156 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 845 875 892 922 1047
Size: 845 30 17 30 125
Fragments arranged by size:
845 125 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 895 1047
Size: 52 843 152
Fragments arranged by size:
843 152 52
BseYI C'CCAG_C
Cuts at: 0 718 954 1047
Size: 718 236 93
Fragments arranged by size:
718 236 93
BsiHKAI G_wGCw'C
Cuts at: 0 656 1047
Size: 656 391
BsmI GAATG_Cn'
Cuts at: 0 335 1047
Size: 335 712
Bsp1286I G_dGCh'C
Cuts at: 0 71 656 719 1047
Size: 71 585 63 328
Fragments arranged by size:
585 328 71 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 824 1047
Size: 824 223
BsrFI r'CCGG_y
Cuts at: 0 3 636 1047
Size: 3 633 411
Fragments arranged by size:
633 411 3
BstEII G'GTnAC_C
Cuts at: 0 612 867 1047
Size: 612 255 180
Fragments arranged by size:
612 255 180
BstYI r'GATC_y
Cuts at: 0 104 668 1047
Size: 104 564 379
Fragments arranged by size:
564 379 104
BtgI C'CryG_G
Cuts at: 0 28 1047
Size: 28 1019
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 737 1047
Size: 737 310
DrdI GACnn_nn'nnGTC
Cuts at: 0 865 1047
Size: 865 182
EaeI y'GGCC_r
Cuts at: 0 745 973 1047
Size: 745 228 74
Fragments arranged by size:
745 228 74
EarI CTCTTCn'nnn_
Cuts at: 0 722 754 1047
Size: 722 32 293
Fragments arranged by size:
722 293 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 575 771 1047
Size: 575 196 276
Fragments arranged by size:
575 276 196
EcoICRI GAG'CTC
Cuts at: 0 654 1047
Size: 654 393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 300 847 1047
Size: 55 245 547 200
Fragments arranged by size:
547 245 200 55
EcoRI G'AATT_C
Cuts at: 0 255 1047
Size: 255 792
EcoRV GAT'ATC
Cuts at: 0 1020 1047
Size: 1020 27
FspI TGC'GCA
Cuts at: 0 19 1047
Size: 19 1028
FspAI rTGC'GCAy
Cuts at: 0 19 1047
Size: 19 1028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 872 1047
Size: 77 795 175
Fragments arranged by size:
795 175 77
NsiI A_TGCA'T
Cuts at: 0 119 1047
Size: 119 928
NspI r_CATG'y
Cuts at: 0 24 121 1047
Size: 24 97 926
Fragments arranged by size:
926 97 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 227 1047
Size: 227 820
PspXI vC'TCGA_Gb
Cuts at: 0 803 1047
Size: 803 244
PstI C_TGCA'G
Cuts at: 0 668 1047
Size: 668 379
SacI G_AGCT'C
Cuts at: 0 656 1047
Size: 656 391
SfcI C'TryA_G
Cuts at: 0 664 1047
Size: 664 383
SmaI CCC'GGG
Cuts at: 0 65 991 1047
Size: 65 926 56
Fragments arranged by size:
926 65 56
SmlI C'TyrA_G
Cuts at: 0 70 803 1047
Size: 70 733 244
Fragments arranged by size:
733 244 70
SphI G_CATG'C
Cuts at: 0 24 121 1047
Size: 24 97 926
Fragments arranged by size:
926 97 24
SspI AAT'ATT
Cuts at: 0 768 1047
Size: 768 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 122 1039 1047
Size: 122 917 8
Fragments arranged by size:
917 122 8
XhoI C'TCGA_G
Cuts at: 0 803 1047
Size: 803 244
XmaI C'CCGG_G
Cuts at: 0 63 989 1047
Size: 63 926 58
Fragments arranged by size:
926 63 58
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApoI AvaI BanI BanII
BbsI BbvCI BfrBI BglI BglII Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspMI BsrFI BstEII BstYI BtgI
BtgZI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoRI
EcoRV FspI FspAI MmeI NsiI NspI PflMI PspXI
PstI SacI SfcI SmaI SmlI SphI SspI TaqII
XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BlpI
BmgBI BmtI BplI BsaAI BsaBI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII EagI EcoNI EcoO109I FalI FseI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
StuI StyI SwaI TatI TstI Tth111I XbaI XcmI
XmnI ZraI