Restriction map for
pF1KB7707
(Linear) (Six-base) MAPSORT of: KIBB7707.seq Check: 587 from: 1 to: 1257
KIBB7707.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 382 1257
Size: 382 875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 1257
Size: 114 1143
AhdI GACnn_n'nnGTC
Cuts at: 0 870 1257
Size: 870 387
AleI CACnn'nnGTG
Cuts at: 0 890 981 1257
Size: 890 91 276
Fragments arranged by size:
890 276 91
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 475 1257
Size: 475 782
AlwNI CAG_nnn'CTG
Cuts at: 0 708 804 918 1257
Size: 708 96 114 339
Fragments arranged by size:
708 339 114 96
AvaI C'yCGr_G
Cuts at: 0 962 1257
Size: 962 295
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 263 296 1257
Size: 263 33 961
Fragments arranged by size:
961 263 33
BanII G_rGCy'C
Cuts at: 0 1215 1257
Size: 1215 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 526 590 1257
Size: 56 470 64 667
Fragments arranged by size:
667 470 64 56
BciVI GTATCCnnnnn_n'
Cuts at: 0 264 1257
Size: 264 993
BlpI GC'TnA_GC
Cuts at: 0 830 1257
Size: 830 427
BmgBI CAC'GTC
Cuts at: 0 641 1257
Size: 641 616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 1157 1257
Size: 886 271 100
Fragments arranged by size:
886 271 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 220 224 952 1257
Size: 220 4 728 305
Fragments arranged by size:
728 305 220 4
BsaAI yAC'GTr
Cuts at: 0 579 1257
Size: 579 678
BsaHI Gr'CG_yC
Cuts at: 0 784 1257
Size: 784 473
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 16 46 200 230 1257
Size: 16 30 154 30 1027
Fragments arranged by size:
1027 154 30 30 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 1257
Size: 399 858
BsiEI CG_ry'CG
Cuts at: 0 1051 1257
Size: 1051 206
BsiHKAI G_wGCw'C
Cuts at: 0 85 1257
Size: 85 1172
BsmBI CGTCTCn'nnnn_
Cuts at: 0 631 1257
Size: 631 626
Bsp1286I G_dGCh'C
Cuts at: 0 85 1215 1257
Size: 85 1130 42
Fragments arranged by size:
1130 85 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 382 779 1257
Size: 382 397 478
Fragments arranged by size:
478 397 382
BsrBI CCG'CTC
Cuts at: 0 322 926 1257
Size: 322 604 331
Fragments arranged by size:
604 331 322
BsrFI r'CCGG_y
Cuts at: 0 1050 1257
Size: 1050 207
BssSI C'ACGA_G
Cuts at: 0 1236 1257
Size: 1236 21
BstAPI GCAn_nnn'nTGC
Cuts at: 0 666 708 804 1257
Size: 666 42 96 453
Fragments arranged by size:
666 453 96 42
BstEII G'GTnAC_C
Cuts at: 0 102 648 892 1257
Size: 102 546 244 365
Fragments arranged by size:
546 365 244 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 42 579 983 1257
Size: 42 537 404 274
Fragments arranged by size:
537 404 274 42
BstYI r'GATC_y
Cuts at: 0 1103 1257
Size: 1103 154
Bsu36I CC'TnA_GG
Cuts at: 0 1026 1187 1257
Size: 1026 161 70
Fragments arranged by size:
1026 161 70
BtgI C'CryG_G
Cuts at: 0 35 275 1257
Size: 35 240 982
Fragments arranged by size:
982 240 35
BtsI GCAGTG_nn'
Cuts at: 0 677 1257
Size: 677 580
EaeI y'GGCC_r
Cuts at: 0 323 1048 1052 1257
Size: 323 725 4 205
Fragments arranged by size:
725 323 205 4
EagI C'GGCC_G
Cuts at: 0 1048 1257
Size: 1048 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 886 1157 1257
Size: 114 772 271 100
Fragments arranged by size:
772 271 114 100
EcoO109I rG'GnC_Cy
Cuts at: 0 114 1257
Size: 114 1143
FseI GG_CCGG'CC
Cuts at: 0 1054 1257
Size: 1054 203
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 478 510 588 620 856 888
Size: 16 32 430 32 78 32 236 32
Cuts at: 888 1257
Size: 369
Fragments arranged by size:
430 369 236 78 32 32 32 32
16
HindIII A'AGCT_T
Cuts at: 0 551 1257
Size: 551 706
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1257
Size: 110 1147
MslI CAynn'nnrTG
Cuts at: 0 457 498 890 981 1257
Size: 457 41 392 91 276
Fragments arranged by size:
457 392 276 91 41
MspA1I CmG'CkG
Cuts at: 0 17 75 277 837 1257
Size: 17 58 202 560 420
Fragments arranged by size:
560 420 202 58 17
NaeI GCC'GGC
Cuts at: 0 1052 1257
Size: 1052 205
NgoMIV G'CCGG_C
Cuts at: 0 1050 1257
Size: 1050 207
NspI r_CATG'y
Cuts at: 0 462 1086 1257
Size: 462 624 171
Fragments arranged by size:
624 462 171
PasI CC'CwG_GG
Cuts at: 0 1038 1257
Size: 1038 219
PflMI CCAn_nnn'nTGG
Cuts at: 0 918 1257
Size: 918 339
PpuMI rG'GwC_Cy
Cuts at: 0 114 1257
Size: 114 1143
PshAI GACnn'nnGTC
Cuts at: 0 236 1257
Size: 236 1021
PstI C_TGCA'G
Cuts at: 0 251 713 720 800 1257
Size: 251 462 7 80 457
Fragments arranged by size:
462 457 251 80 7
PvuII CAG'CTG
Cuts at: 0 75 837 1257
Size: 75 762 420
Fragments arranged by size:
762 420 75
SacII CC_GC'GG
Cuts at: 0 278 1257
Size: 278 979
SbfI CC_TGCA'GG
Cuts at: 0 800 1257
Size: 800 457
SfcI C'TryA_G
Cuts at: 0 247 709 716 796 1257
Size: 247 462 7 80 461
Fragments arranged by size:
462 461 247 80 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 344 1257
Size: 344 913
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 473 505 1257
Size: 473 32 752
Fragments arranged by size:
752 473 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 42 1257
Size: 42 1215
XmnI GAAnn'nnTTC
Cuts at: 0 1020 1257
Size: 1020 237
Enzymes that do cut:
AarI AcuI AhdI AleI AlfI AlwNI AvaI BaeI
BanII BbsI BciVI BlpI BmgBI BpmI BsaI BsaAI
BsaHI BsaXI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI EaeI EagI Eco57MI EcoO109I FseI Hin4I
HindIII MmeI MslI MspA1I NaeI NgoMIV NspI PasI
PflMI PpuMI PshAI PstI PvuII SacII SbfI SfcI
TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BanI BbeI BbvCI BcgI BclI BfrBI BglI
BglII Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaBI
BsaWI BseRI BseYI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BssHII BstBI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MscI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TatI Tth111I XbaI XhoI XmaI ZraI