Restriction map for
pF1KB7713
(Linear) (Six-base) MAPSORT of: KIBB7713.seq Check: 2220 from: 1 to: 1269
KIBB7713.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AfeI AGC'GCT
Cuts at: 0 826 1269
Size: 826 443
AhdI GACnn_n'nnGTC
Cuts at: 0 771 1269
Size: 771 498
AleI CACnn'nnGTG
Cuts at: 0 272 1269
Size: 272 997
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1159 1269
Size: 1159 110
AlwNI CAG_nnn'CTG
Cuts at: 0 550 652 954 1015 1269
Size: 550 102 302 61 254
Fragments arranged by size:
550 302 254 102 61
ApaI G_GGCC'C
Cuts at: 0 523 539 1269
Size: 523 16 730
Fragments arranged by size:
730 523 16
ApaLI G'TGCA_C
Cuts at: 0 1018 1269
Size: 1018 251
AvaI C'yCGr_G
Cuts at: 0 516 1252 1269
Size: 516 736 17
Fragments arranged by size:
736 516 17
BanI G'GyrC_C
Cuts at: 0 659 1221 1269
Size: 659 562 48
Fragments arranged by size:
659 562 48
BanII G_rGCy'C
Cuts at: 0 523 539 1269
Size: 523 16 730
Fragments arranged by size:
730 523 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 571 1269
Size: 571 698
BbvCI CC'TCA_GC
Cuts at: 0 860 1269
Size: 860 409
BglI GCCn_nnn'nGGC
Cuts at: 0 143 394 1096 1100 1269
Size: 143 251 702 4 169
Fragments arranged by size:
702 251 169 143 4
BlpI GC'TnA_GC
Cuts at: 0 211 1118 1269
Size: 211 907 151
Fragments arranged by size:
907 211 151
Bme1580I G_kGCm'C
Cuts at: 0 523 539 662 891 1022 1226 1269
Size: 523 16 123 229 131 204 43
Fragments arranged by size:
523 229 204 131 123 43 16
BmgBI CAC'GTC
Cuts at: 0 252 1269
Size: 252 1017
BmrI ACTGGGnnnn_n'
Cuts at: 0 571 922 1269
Size: 571 351 347
Fragments arranged by size:
571 351 347
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 982 1099 1231 1269
Size: 572 410 117 132 38
Fragments arranged by size:
572 410 132 117 38
Bpu10I CC'TnA_GC
Cuts at: 0 860 1269
Size: 860 409
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1269
Size: 418 851
BsaI GGTCTCn'nnnn_
Cuts at: 0 82 1269
Size: 82 1187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 81 111 241 271 1145 1175 1269
Size: 81 30 130 30 874 30 94
Fragments arranged by size:
874 130 94 81 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 172 985 1150 1269
Size: 172 813 165 119
Fragments arranged by size:
813 172 165 119
BseYI C'CCAG_C
Cuts at: 0 175 532 666 722 810 821 1269
Size: 175 357 134 56 88 11 448
Fragments arranged by size:
448 357 175 134 88 56 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1269
Size: 1048 221
BsiEI CG_ry'CG
Cuts at: 0 493 1095 1269
Size: 493 602 174
Fragments arranged by size:
602 493 174
BsiHKAI G_wGCw'C
Cuts at: 0 212 1022 1269
Size: 212 810 247
Fragments arranged by size:
810 247 212
BsmI GAATG_Cn'
Cuts at: 0 588 1269
Size: 588 681
BsmBI CGTCTCn'nnnn_
Cuts at: 0 372 1269
Size: 372 897
Bsp1286I G_dGCh'C
Cuts at: 0 212 523 539 662 891 1022 1226 1269
Size: 212 311 16 123 229 131 204 43
Fragments arranged by size:
311 229 212 204 131 123 43 16
BsrBI CCG'CTC
Cuts at: 0 1122 1158 1269
Size: 1122 36 111
Fragments arranged by size:
1122 111 36
BsrFI r'CCGG_y
Cuts at: 0 48 1090 1269
Size: 48 1042 179
Fragments arranged by size:
1042 179 48
BssSI C'ACGA_G
Cuts at: 0 1066 1269
Size: 1066 203
BstAPI GCAn_nnn'nTGC
Cuts at: 0 389 936 954 1269
Size: 389 547 18 315
Fragments arranged by size:
547 389 315 18
BstEII G'GTnAC_C
Cuts at: 0 711 1269
Size: 711 558
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 1198 1269
Size: 12 1186 71
Fragments arranged by size:
1186 71 12
BtgI C'CryG_G
Cuts at: 0 671 1269
Size: 671 598
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 734 1269
Size: 734 535
BtsI GCAGTG_nn'
Cuts at: 0 505 829 835 1269
Size: 505 324 6 434
Fragments arranged by size:
505 434 324 6
DraIII CAC_nnn'GTG
Cuts at: 0 413 1269
Size: 413 856
EaeI y'GGCC_r
Cuts at: 0 50 490 1092 1269
Size: 50 440 602 177
Fragments arranged by size:
602 440 177 50
EagI C'GGCC_G
Cuts at: 0 490 1092 1269
Size: 490 602 177
Fragments arranged by size:
602 490 177
EarI CTCTTCn'nnn_
Cuts at: 0 149 875 1269
Size: 149 726 394
Fragments arranged by size:
726 394 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1153 1269
Size: 1153 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 982 1099 1231 1269
Size: 572 410 117 132 38
Fragments arranged by size:
572 410 132 117 38
EcoO109I rG'GnC_Cy
Cuts at: 0 520 536 556 649 951 1071 1269
Size: 520 16 20 93 302 120 198
Fragments arranged by size:
520 302 198 120 93 20 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1257 1269
Size: 1257 12
HaeII r_GCGC'y
Cuts at: 0 231 828 1269
Size: 231 597 441
Fragments arranged by size:
597 441 231
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 81 113 241 273 757 789 1269
Size: 81 32 128 32 484 32 480
Fragments arranged by size:
484 480 128 81 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 1269
Size: 377 892
MslI CAynn'nnrTG
Cuts at: 0 272 1196 1269
Size: 272 924 73
Fragments arranged by size:
924 272 73
MspA1I CmG'CkG
Cuts at: 0 341 722 898 1050 1174 1269
Size: 341 381 176 152 124 95
Fragments arranged by size:
381 341 176 152 124 95
NaeI GCC'GGC
Cuts at: 0 50 1092 1269
Size: 50 1042 177
Fragments arranged by size:
1042 177 50
NgoMIV G'CCGG_C
Cuts at: 0 48 1090 1269
Size: 48 1042 179
Fragments arranged by size:
1042 179 48
NspI r_CATG'y
Cuts at: 0 432 1269
Size: 432 837
PflMI CCAn_nnn'nTGG
Cuts at: 0 15 722 1269
Size: 15 707 547
Fragments arranged by size:
707 547 15
PfoI T'CCnGG_A
Cuts at: 0 872 1269
Size: 872 397
PspOMI G'GGCC_C
Cuts at: 0 519 535 1269
Size: 519 16 734
Fragments arranged by size:
734 519 16
PspXI vC'TCGA_Gb
Cuts at: 0 1252 1269
Size: 1252 17
PstI C_TGCA'G
Cuts at: 0 785 842 950 1269
Size: 785 57 108 319
Fragments arranged by size:
785 319 108 57
PvuII CAG'CTG
Cuts at: 0 341 722 898 1050 1269
Size: 341 381 176 152 219
Fragments arranged by size:
381 341 219 176 152
ScaI AGT'ACT
Cuts at: 0 779 1269
Size: 779 490
SfcI C'TryA_G
Cuts at: 0 781 838 946 1269
Size: 781 57 108 323
Fragments arranged by size:
781 323 108 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 394 1100 1269
Size: 394 706 169
Fragments arranged by size:
706 394 169
SmaI CCC'GGG
Cuts at: 0 518 1269
Size: 518 751
SmlI C'TyrA_G
Cuts at: 0 433 1252 1269
Size: 433 819 17
Fragments arranged by size:
819 433 17
SpeI A'CTAG_T
Cuts at: 0 933 1269
Size: 933 336
SphI G_CATG'C
Cuts at: 0 432 1269
Size: 432 837
StuI AGG'CCT
Cuts at: 0 292 388 397 991 1269
Size: 292 96 9 594 278
Fragments arranged by size:
594 292 278 96 9
StyI C'CwwG_G
Cuts at: 0 992 1269
Size: 992 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 106 713 802 1269
Size: 106 607 89 467
Fragments arranged by size:
607 467 106 89
TatI w'GTAC_w
Cuts at: 0 335 479 777 1269
Size: 335 144 298 492
Fragments arranged by size:
492 335 298 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 497 529 1269
Size: 497 32 740
Fragments arranged by size:
740 497 32
Tth111I GACn'n_nGTC
Cuts at: 0 457 868 1269
Size: 457 411 401
Fragments arranged by size:
457 411 401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 12 1269
Size: 12 1257
XhoI C'TCGA_G
Cuts at: 0 1252 1269
Size: 1252 17
XmaI C'CCGG_G
Cuts at: 0 516 1269
Size: 516 753
Enzymes that do cut:
AfeI AhdI AleI AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbsI BbvCI BglI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrFI
BssSI BstAPI BstEII BstXI BtgI BtgZI BtsI DraIII
EaeI EagI EarI EciI Eco57MI EcoO109I FalI HaeII
Hin4I MmeI MslI MspA1I NaeI NgoMIV NspI PflMI
PfoI PspOMI PspXI PstI PvuII ScaI SfcI SfiI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglII BmtI
BplI BsaAI BsaBI BsaHI BsaWI BsiWI BspEI BspHI
BspMI BsrDI BsrGI BssHII BstBI BstYI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfoI SgfI SgrAI
SnaBI SrfI SspI SwaI XbaI XmnI ZraI