Restriction map for
pF1KB7714
(Linear) (Six-base) MAPSORT of: KIBB7714.seq Check: 4447 from: 1 to: 1419
KIBB7714.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 57 1042 1419
Size: 57 985 377
Fragments arranged by size:
985 377 57
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 964 1126 1255 1309 1419
Size: 891 73 162 129 54 110
Fragments arranged by size:
891 162 129 110 73 54
AflIII A'CryG_T
Cuts at: 0 286 851 863 1419
Size: 286 565 12 556
Fragments arranged by size:
565 556 286 12
AhdI GACnn_n'nnGTC
Cuts at: 0 177 1419
Size: 177 1242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 86 1419
Size: 86 1333
AlwNI CAG_nnn'CTG
Cuts at: 0 181 528 543 659 782 1347 1419
Size: 181 347 15 116 123 565 72
Fragments arranged by size:
565 347 181 123 116 72 15
ApoI r'AATT_y
Cuts at: 0 627 1090 1419
Size: 627 463 329
Fragments arranged by size:
627 463 329
BanI G'GyrC_C
Cuts at: 0 344 361 1419
Size: 344 17 1058
Fragments arranged by size:
1058 344 17
BanII G_rGCy'C
Cuts at: 0 1082 1316 1419
Size: 1082 234 103
Fragments arranged by size:
1082 234 103
BbeI G_GCGC'C
Cuts at: 0 365 1419
Size: 365 1054
BbsI GAAGACnn'nnnn_
Cuts at: 0 388 1049 1419
Size: 388 661 370
Fragments arranged by size:
661 388 370
BbvCI CC'TCA_GC
Cuts at: 0 340 1419
Size: 340 1079
BclI T'GATC_A
Cuts at: 0 449 488 566 1419
Size: 449 39 78 853
Fragments arranged by size:
853 449 78 39
BglII A'GATC_T
Cuts at: 0 729 821 932 1419
Size: 729 92 111 487
Fragments arranged by size:
729 487 111 92
BlpI GC'TnA_GC
Cuts at: 0 649 1331 1419
Size: 649 682 88
Fragments arranged by size:
682 649 88
Bme1580I G_kGCm'C
Cuts at: 0 349 1419
Size: 349 1070
BmrI ACTGGGnnnn_n'
Cuts at: 0 191 538 1419
Size: 191 347 881
Fragments arranged by size:
881 347 191
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 843 1419
Size: 843 576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 445 1096 1419
Size: 139 306 651 323
Fragments arranged by size:
651 323 306 139
Bpu10I CC'TnA_GC
Cuts at: 0 340 1419
Size: 340 1079
BsaI GGTCTCn'nnnn_
Cuts at: 0 120 753 1419
Size: 120 633 666
Fragments arranged by size:
666 633 120
BsaBI GATnn'nnATC
Cuts at: 0 565 1419
Size: 565 854
BsaHI Gr'CG_yC
Cuts at: 0 362 1419
Size: 362 1057
BsaWI w'CCGG_w
Cuts at: 0 353 722 1419
Size: 353 369 697
Fragments arranged by size:
697 369 353
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 919 949 1419
Size: 919 30 470
Fragments arranged by size:
919 470 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 487 565 1141 1144 1419
Size: 487 78 576 3 275
Fragments arranged by size:
576 487 275 78 3
BseYI C'CCAG_C
Cuts at: 0 798 1262 1340 1419
Size: 798 464 78 79
Fragments arranged by size:
798 464 79 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 556 1419
Size: 46 510 863
Fragments arranged by size:
863 510 46
BsiHKAI G_wGCw'C
Cuts at: 0 48 739 1316 1419
Size: 48 691 577 103
Fragments arranged by size:
691 577 103 48
BsmI GAATG_Cn'
Cuts at: 0 1255 1419
Size: 1255 164
Bsp1286I G_dGCh'C
Cuts at: 0 48 349 739 1082 1316 1419
Size: 48 301 390 343 234 103
Fragments arranged by size:
390 343 301 234 103 48
BspEI T'CCGG_A
Cuts at: 0 353 722 1419
Size: 353 369 697
Fragments arranged by size:
697 369 353
BspHI T'CATG_A
Cuts at: 0 281 1419
Size: 281 1138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 57 1042 1419
Size: 57 985 377
Fragments arranged by size:
985 377 57
BsrBI CCG'CTC
Cuts at: 0 431 1419
Size: 431 988
BsrFI r'CCGG_y
Cuts at: 0 1022 1419
Size: 1022 397
BsrGI T'GTAC_A
Cuts at: 0 159 1419
Size: 159 1260
BstAPI GCAn_nnn'nTGC
Cuts at: 0 659 1419
Size: 659 760
BstYI r'GATC_y
Cuts at: 0 729 821 932 1419
Size: 729 92 111 487
Fragments arranged by size:
729 487 111 92
BtgI C'CryG_G
Cuts at: 0 467 1419
Size: 467 952
BtsI GCAGTG_nn'
Cuts at: 0 660 783 1046 1419
Size: 660 123 263 373
Fragments arranged by size:
660 373 263 123
DraIII CAC_nnn'GTG
Cuts at: 0 1197 1419
Size: 1197 222
EaeI y'GGCC_r
Cuts at: 0 1020 1419
Size: 1020 399
EarI CTCTTCn'nnn_
Cuts at: 0 1165 1419
Size: 1165 254
EcoICRI GAG'CTC
Cuts at: 0 1314 1419
Size: 1314 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 445 891 964 1096 1126 1255 1309
Size: 139 306 446 73 132 30 129 54
Cuts at: 1309 1419
Size: 110
Fragments arranged by size:
446 306 139 132 129 110 73 54
30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 906 943 1390 1419
Size: 906 37 447 29
Fragments arranged by size:
906 447 37 29
EcoO109I rG'GnC_Cy
Cuts at: 0 35 226 684 1419
Size: 35 191 458 735
Fragments arranged by size:
735 458 191 35
FspI TGC'GCA
Cuts at: 0 415 1354 1419
Size: 415 939 65
Fragments arranged by size:
939 415 65
HaeII r_GCGC'y
Cuts at: 0 365 1419
Size: 365 1054
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 163 195 504 536 570 602 721 753
Size: 163 32 309 32 34 32 119 32
Cuts at: 753 919 951 1419
Size: 166 32 468
Fragments arranged by size:
468 309 166 163 119 34 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 204 857 970 1419
Size: 204 653 113 449
Fragments arranged by size:
653 449 204 113
KasI G'GCGC_C
Cuts at: 0 361 1419
Size: 361 1058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 482 1419
Size: 201 281 937
Fragments arranged by size:
937 281 201
MslI CAynn'nnrTG
Cuts at: 0 275 1300 1419
Size: 275 1025 119
Fragments arranged by size:
1025 275 119
MspA1I CmG'CkG
Cuts at: 0 558 759 1119 1209 1344 1419
Size: 558 201 360 90 135 75
Fragments arranged by size:
558 360 201 135 90 75
NaeI GCC'GGC
Cuts at: 0 1024 1419
Size: 1024 395
NarI GG'CG_CC
Cuts at: 0 362 1419
Size: 362 1057
NgoMIV G'CCGG_C
Cuts at: 0 1022 1419
Size: 1022 397
NspI r_CATG'y
Cuts at: 0 290 855 867 1104 1419
Size: 290 565 12 237 315
Fragments arranged by size:
565 315 290 237 12
PciI A'CATG_T
Cuts at: 0 286 851 863 1419
Size: 286 565 12 556
Fragments arranged by size:
565 556 286 12
PfoI T'CCnGG_A
Cuts at: 0 186 1358 1419
Size: 186 1172 61
Fragments arranged by size:
1172 186 61
PpuMI rG'GwC_Cy
Cuts at: 0 35 226 1419
Size: 35 191 1193
Fragments arranged by size:
1193 191 35
PstI C_TGCA'G
Cuts at: 0 683 758 1053 1118 1190 1214 1419
Size: 683 75 295 65 72 24 205
Fragments arranged by size:
683 295 205 75 72 65 24
PvuII CAG'CTG
Cuts at: 0 759 1119 1209 1419
Size: 759 360 90 210
Fragments arranged by size:
759 360 210 90
SacI G_AGCT'C
Cuts at: 0 1316 1419
Size: 1316 103
SanDI GG'GwC_CC
Cuts at: 0 35 1419
Size: 35 1384
SfcI C'TryA_G
Cuts at: 0 679 754 1049 1114 1186 1210 1419
Size: 679 75 295 65 72 24 209
Fragments arranged by size:
679 295 209 75 72 65 24
SfoI GGC'GCC
Cuts at: 0 363 1419
Size: 363 1056
StuI AGG'CCT
Cuts at: 0 403 1419
Size: 403 1016
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1354 1419
Size: 1354 65
TatI w'GTAC_w
Cuts at: 0 159 1419
Size: 159 1260
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 489 521 1419
Size: 489 32 898
Fragments arranged by size:
898 489 32
Enzymes that do cut:
AarI AcuI AflIII AhdI AlfI AlwNI ApoI BanI
BanII BbeI BbsI BbvCI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BsrGI BstAPI BstYI BtgI
BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I HincII KasI MmeI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PpuMI PstI
PvuII SacI SanDI SfcI SfoI StuI TaqII TatI
TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BcgI BciVI BfrBI BglI BmgBI BmtI
BpuEI BsaAI BsiEI BsiWI BsmBI BsrDI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoRI EcoRV FalI FseI
FspAI HindIII HpaI KpnI MfeI MluI MscI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StyI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI