Restriction map for
pF1KB7717
(Linear) (Six-base) MAPSORT of: KIBB7717.seq Check: 8218 from: 1 to: 1275
KIBB7717.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 407 612 1275
Size: 290 117 205 663
Fragments arranged by size:
663 290 205 117
AhdI GACnn_n'nnGTC
Cuts at: 0 723 1275
Size: 723 552
AlwNI CAG_nnn'CTG
Cuts at: 0 530 1275
Size: 530 745
ApoI r'AATT_y
Cuts at: 0 370 494 746 830 1275
Size: 370 124 252 84 445
Fragments arranged by size:
445 370 252 124 84
AvaI C'yCGr_G
Cuts at: 0 452 1275
Size: 452 823
BanI G'GyrC_C
Cuts at: 0 1142 1275
Size: 1142 133
BanII G_rGCy'C
Cuts at: 0 682 1275
Size: 682 593
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 544 1275
Size: 56 488 731
Fragments arranged by size:
731 488 56
BbvCI CC'TCA_GC
Cuts at: 0 646 1275
Size: 646 629
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 37 71 1275
Size: 37 34 1204
Fragments arranged by size:
1204 37 34
BglII A'GATC_T
Cuts at: 0 225 1275
Size: 225 1050
BmrI ACTGGGnnnn_n'
Cuts at: 0 285 596 680 1275
Size: 285 311 84 595
Fragments arranged by size:
595 311 285 84
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 282 284 1275
Size: 282 2 991
Fragments arranged by size:
991 282 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 776 860 1112 1275
Size: 151 625 84 252 163
Fragments arranged by size:
625 252 163 151 84
Bpu10I CC'TnA_GC
Cuts at: 0 646 1275
Size: 646 629
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 1275
Size: 256 1019
BseYI C'CCAG_C
Cuts at: 0 1006 1275
Size: 1006 269
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 711 1275
Size: 711 564
BsiHKAI G_wGCw'C
Cuts at: 0 703 1275
Size: 703 572
BsmI GAATG_Cn'
Cuts at: 0 343 526 1114 1123 1198 1275
Size: 343 183 588 9 75 77
Fragments arranged by size:
588 343 183 77 75 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 983 1275
Size: 983 292
Bsp1286I G_dGCh'C
Cuts at: 0 682 703 1275
Size: 682 21 572
Fragments arranged by size:
682 572 21
BsrBI CCG'CTC
Cuts at: 0 1058 1275
Size: 1058 217
BsrFI r'CCGG_y
Cuts at: 0 1145 1275
Size: 1145 130
BstAPI GCAn_nnn'nTGC
Cuts at: 0 698 1118 1275
Size: 698 420 157
Fragments arranged by size:
698 420 157
BstBI TT'CG_AA
Cuts at: 0 47 1275
Size: 47 1228
BstYI r'GATC_y
Cuts at: 0 225 1082 1275
Size: 225 857 193
Fragments arranged by size:
857 225 193
BtgI C'CryG_G
Cuts at: 0 59 214 1275
Size: 59 155 1061
Fragments arranged by size:
1061 155 59
BtsI GCAGTG_nn'
Cuts at: 0 531 699 1275
Size: 531 168 576
Fragments arranged by size:
576 531 168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 765 800 1275
Size: 765 35 475
Fragments arranged by size:
765 475 35
DraIII CAC_nnn'GTG
Cuts at: 0 1202 1275
Size: 1202 73
EaeI y'GGCC_r
Cuts at: 0 253 1275
Size: 253 1022
EarI CTCTTCn'nnn_
Cuts at: 0 71 193 1275
Size: 71 122 1082
Fragments arranged by size:
1082 122 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 290 407 612 776 860 1112 1275
Size: 151 139 117 205 164 84 252 163
Fragments arranged by size:
252 205 164 163 151 139 117 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 1275
Size: 115 1160
EcoO109I rG'GnC_Cy
Cuts at: 0 108 1011 1275
Size: 108 903 264
Fragments arranged by size:
903 264 108
EcoRI G'AATT_C
Cuts at: 0 746 830 1275
Size: 746 84 445
Fragments arranged by size:
746 445 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 210 242 301 333 541 573 709 741
Size: 210 32 59 32 208 32 136 32
Cuts at: 741 793 825 1045 1077 1275
Size: 52 32 220 32 198
Fragments arranged by size:
220 210 208 198 136 59 52 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 959 1043 1275
Size: 959 84 232
Fragments arranged by size:
959 232 84
MfeI C'AATT_G
Cuts at: 0 193 1275
Size: 193 1082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1275
Size: 40 1235
MscI TGG'CCA
Cuts at: 0 255 1275
Size: 255 1020
MslI CAynn'nnrTG
Cuts at: 0 866 1107 1163 1275
Size: 866 241 56 112
Fragments arranged by size:
866 241 112 56
MspA1I CmG'CkG
Cuts at: 0 1140 1275
Size: 1140 135
NaeI GCC'GGC
Cuts at: 0 1147 1275
Size: 1147 128
NdeI CA'TA_TG
Cuts at: 0 1104 1275
Size: 1104 171
NgoMIV G'CCGG_C
Cuts at: 0 1145 1275
Size: 1145 130
PpuMI rG'GwC_Cy
Cuts at: 0 108 1011 1275
Size: 108 903 264
Fragments arranged by size:
903 264 108
SanDI GG'GwC_CC
Cuts at: 0 1011 1275
Size: 1011 264
SmaI CCC'GGG
Cuts at: 0 454 1275
Size: 454 821
StyI C'CwwG_G
Cuts at: 0 342 1275
Size: 342 933
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 402 1275
Size: 402 873
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 199 231 1275
Size: 199 32 1044
Fragments arranged by size:
1044 199 32
XbaI T'CTAG_A
Cuts at: 0 270 1275
Size: 270 1005
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 1275
Size: 396 879
XmaI C'CCGG_G
Cuts at: 0 452 1275
Size: 452 823
XmnI GAAnn'nnTTC
Cuts at: 0 625 961 1213 1275
Size: 625 336 252 62
Fragments arranged by size:
625 336 252 62
Enzymes that do cut:
AcuI AhdI AlwNI ApoI AvaI BanI BanII BbsI
BbvCI BcgI BglII BmrI BplI BpmI Bpu10I BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrFI
BstAPI BstBI BstYI BtgI BtsI CspCI DraIII EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I HindIII MfeI
MmeI MscI MslI MspA1I NaeI NdeI NgoMIV PpuMI
SanDI SmaI StyI TaqII TstI XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmtI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BspEI BspHI
BspMI BsrDI BsrGI BssHII BssSI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI DraI DrdI EagI EciI EcoICRI
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MluI NarI NcoI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XhoI ZraI