Restriction map for
pF1KB7728
(Linear) (Six-base) MAPSORT of: KIBB7728.seq Check: 6275 from: 1 to: 1437
KIBB7728.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 629 920 1437
Size: 629 291 517
Fragments arranged by size:
629 517 291
AccI GT'mk_AC
Cuts at: 0 331 1295 1437
Size: 331 964 142
Fragments arranged by size:
964 331 142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1036 1437
Size: 1036 401
AflIII A'CryG_T
Cuts at: 0 581 1437
Size: 581 856
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 73 1437
Size: 73 1364
AlwNI CAG_nnn'CTG
Cuts at: 0 348 1006 1134 1437
Size: 348 658 128 303
Fragments arranged by size:
658 348 303 128
ApaI G_GGCC'C
Cuts at: 0 1309 1437
Size: 1309 128
ApoI r'AATT_y
Cuts at: 0 523 1437
Size: 523 914
AvaI C'yCGr_G
Cuts at: 0 22 112 1025 1437
Size: 22 90 913 412
Fragments arranged by size:
913 412 90 22
BamHI G'GATC_C
Cuts at: 0 383 1064 1437
Size: 383 681 373
Fragments arranged by size:
681 383 373
BanII G_rGCy'C
Cuts at: 0 1309 1437
Size: 1309 128
BbsI GAAGACnn'nnnn_
Cuts at: 0 300 688 1437
Size: 300 388 749
Fragments arranged by size:
749 388 300
BbvCI CC'TCA_GC
Cuts at: 0 1378 1437
Size: 1378 59
BglI GCCn_nnn'nGGC
Cuts at: 0 1313 1437
Size: 1313 124
BglII A'GATC_T
Cuts at: 0 267 1043 1437
Size: 267 776 394
Fragments arranged by size:
776 394 267
Bme1580I G_kGCm'C
Cuts at: 0 563 1154 1309 1437
Size: 563 591 155 128
Fragments arranged by size:
591 563 155 128
BmgBI CAC'GTC
Cuts at: 0 164 786 1437
Size: 164 622 651
Fragments arranged by size:
651 622 164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 370 1156 1437
Size: 259 111 786 281
Fragments arranged by size:
786 281 259 111
Bpu10I CC'TnA_GC
Cuts at: 0 1378 1437
Size: 1378 59
BsaWI w'CCGG_w
Cuts at: 0 380 1437
Size: 380 1057
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1255 1285 1437
Size: 1255 30 152
Fragments arranged by size:
1255 152 30
BseYI C'CCAG_C
Cuts at: 0 101 168 252 443 941 1277 1437
Size: 101 67 84 191 498 336 160
Fragments arranged by size:
498 336 191 160 101 84 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1366 1437
Size: 607 759 71
Fragments arranged by size:
759 607 71
BsiEI CG_ry'CG
Cuts at: 0 672 1437
Size: 672 765
BsiHKAI G_wGCw'C
Cuts at: 0 29 71 341 620 772 1437
Size: 29 42 270 279 152 665
Fragments arranged by size:
665 279 270 152 42 29
BsmI GAATG_Cn'
Cuts at: 0 456 493 1437
Size: 456 37 944
Fragments arranged by size:
944 456 37
BsmBI CGTCTCn'nnnn_
Cuts at: 0 184 1437
Size: 184 1253
Bsp1286I G_dGCh'C
Cuts at: 0 29 71 341 563 620 772 1154 1309
Size: 29 42 270 222 57 152 382 155
Cuts at: 1309 1437
Size: 128
Fragments arranged by size:
382 270 222 155 152 128 57 42
29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 65 629 920 1437
Size: 65 564 291 517
Fragments arranged by size:
564 517 291 65
BsrDI GCAATG_nn'
Cuts at: 0 1407 1437
Size: 1407 30
BsrFI r'CCGG_y
Cuts at: 0 320 980 1298 1437
Size: 320 660 318 139
Fragments arranged by size:
660 320 318 139
BsrGI T'GTAC_A
Cuts at: 0 1262 1437
Size: 1262 175
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1048 1215 1437
Size: 1048 167 222
Fragments arranged by size:
1048 222 167
BstYI r'GATC_y
Cuts at: 0 267 383 1043 1064 1437
Size: 267 116 660 21 373
Fragments arranged by size:
660 373 267 116 21
BstZ17I GTA'TAC
Cuts at: 0 332 1437
Size: 332 1105
BtgI C'CryG_G
Cuts at: 0 775 896 1310 1437
Size: 775 121 414 127
Fragments arranged by size:
775 414 127 121
BtsI GCAGTG_nn'
Cuts at: 0 507 909 952 1182 1437
Size: 507 402 43 230 255
Fragments arranged by size:
507 402 255 230 43
ClaI AT'CG_AT
Cuts at: 0 395 1437
Size: 395 1042
DraIII CAC_nnn'GTG
Cuts at: 0 767 882 1437
Size: 767 115 555
Fragments arranged by size:
767 555 115
EaeI y'GGCC_r
Cuts at: 0 370 1437
Size: 370 1067
EarI CTCTTCn'nnn_
Cuts at: 0 10 182 475 1078 1214 1437
Size: 10 172 293 603 136 223
Fragments arranged by size:
603 293 223 172 136 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 370 1036 1156 1437
Size: 259 111 666 120 281
Fragments arranged by size:
666 281 259 120 111
EcoO109I rG'GnC_Cy
Cuts at: 0 471 888 1305 1306 1437
Size: 471 417 417 1 131
Fragments arranged by size:
471 417 417 131 1
EcoRI G'AATT_C
Cuts at: 0 523 1437
Size: 523 914
HaeII r_GCGC'y
Cuts at: 0 809 1437
Size: 809 628
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1035 1067 1437
Size: 1035 32 370
Fragments arranged by size:
1035 370 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 895 1437
Size: 255 640 542
Fragments arranged by size:
640 542 255
MslI CAynn'nnrTG
Cuts at: 0 301 1321 1437
Size: 301 1020 116
Fragments arranged by size:
1020 301 116
MspA1I CmG'CkG
Cuts at: 0 105 348 447 635 1437
Size: 105 243 99 188 802
Fragments arranged by size:
802 243 188 105 99
NaeI GCC'GGC
Cuts at: 0 322 982 1437
Size: 322 660 455
Fragments arranged by size:
660 455 322
NcoI C'CATG_G
Cuts at: 0 775 1437
Size: 775 662
NgoMIV G'CCGG_C
Cuts at: 0 320 980 1437
Size: 320 660 457
Fragments arranged by size:
660 457 320
PasI CC'CwG_GG
Cuts at: 0 428 1437
Size: 428 1009
PflMI CCAn_nnn'nTGG
Cuts at: 0 348 882 1326 1437
Size: 348 534 444 111
Fragments arranged by size:
534 444 348 111
PfoI T'CCnGG_A
Cuts at: 0 386 932 1214 1437
Size: 386 546 282 223
Fragments arranged by size:
546 386 282 223
PpuMI rG'GwC_Cy
Cuts at: 0 471 1437
Size: 471 966
PspOMI G'GGCC_C
Cuts at: 0 1305 1437
Size: 1305 132
PstI C_TGCA'G
Cuts at: 0 329 1160 1437
Size: 329 831 277
Fragments arranged by size:
831 329 277
PvuI CG_AT'CG
Cuts at: 0 672 1437
Size: 672 765
PvuII CAG'CTG
Cuts at: 0 105 348 447 635 1437
Size: 105 243 99 188 802
Fragments arranged by size:
802 243 188 105 99
SanDI GG'GwC_CC
Cuts at: 0 471 1437
Size: 471 966
SapI GCTCTTCn'nnn_
Cuts at: 0 10 1078 1214 1437
Size: 10 1068 136 223
Fragments arranged by size:
1068 223 136 10
SexAI A'CCwGG_T
Cuts at: 0 794 1437
Size: 794 643
SfcI C'TryA_G
Cuts at: 0 325 1156 1437
Size: 325 831 281
Fragments arranged by size:
831 325 281
StyI C'CwwG_G
Cuts at: 0 210 775 1232 1437
Size: 210 565 457 205
Fragments arranged by size:
565 457 210 205
TatI w'GTAC_w
Cuts at: 0 608 1262 1437
Size: 608 654 175
Fragments arranged by size:
654 608 175
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaI ApoI
AvaI BamHI BanII BbsI BbvCI BglI BglII Bme1580I
BmgBI BpmI Bpu10I BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI
BstXI BstYI BstZ17I BtgI BtsI ClaI DraIII EaeI
EarI Eco57MI EcoO109I EcoRI HaeII Hin4I MmeI MslI
MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI PpuMI
PspOMI PstI PvuI PvuII SanDI SapI SexAI SfcI
StyI TatI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BanI
BbeI BcgI BciVI BclI BfrBI BlpI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BseRI BsiWI
BspEI BspHI BsrBI BssHII BssSI BstAPI BstBI BstEII
Bsu36I BtgZI CspCI DraI DrdI EagI EciI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NarI NdeI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI RsrII SacI
SacII SalI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI