Restriction map for
pF1KB7736
(Linear) (Six-base) MAPSORT of: KIBB7736.seq Check: 7981 from: 1 to: 1446
KIBB7736.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AccI GT'mk_AC
Cuts at: 0 337 1446
Size: 337 1109
AclI AA'CG_TT
Cuts at: 0 284 920 1446
Size: 284 636 526
Fragments arranged by size:
636 526 284
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1289 1446
Size: 1289 157
AflIII A'CryG_T
Cuts at: 0 248 1446
Size: 248 1198
AleI CACnn'nnGTG
Cuts at: 0 536 1446
Size: 536 910
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 286 1446
Size: 286 1160
AlwNI CAG_nnn'CTG
Cuts at: 0 622 1095 1446
Size: 622 473 351
Fragments arranged by size:
622 473 351
ApoI r'AATT_y
Cuts at: 0 220 856 1446
Size: 220 636 590
Fragments arranged by size:
636 590 220
BanI G'GyrC_C
Cuts at: 0 16 1446
Size: 16 1430
BbsI GAAGACnn'nnnn_
Cuts at: 0 1199 1446
Size: 1199 247
BbvCI CC'TCA_GC
Cuts at: 0 182 1446
Size: 182 1264
BciVI GTATCCnnnnn_n'
Cuts at: 0 954 1446
Size: 954 492
Bme1580I G_kGCm'C
Cuts at: 0 19 1446
Size: 19 1427
BmrI ACTGGGnnnn_n'
Cuts at: 0 632 1446
Size: 632 814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1446
Size: 1096 350
Bpu10I CC'TnA_GC
Cuts at: 0 182 1446
Size: 182 1264
BsaI GGTCTCn'nnnn_
Cuts at: 0 142 1446
Size: 142 1304
BsaHI Gr'CG_yC
Cuts at: 0 1160 1446
Size: 1160 286
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1423 1446
Size: 1423 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 1186 1215 1288 1375 1399 1414 1444
Size: 199 987 29 73 87 24 15 30
Cuts at: 1444 1446
Size: 2
Fragments arranged by size:
987 199 87 73 30 29 24 15
2
BseYI C'CCAG_C
Cuts at: 0 406 891 1446
Size: 406 485 555
Fragments arranged by size:
555 485 406
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1446
Size: 400 1046
BsmI GAATG_Cn'
Cuts at: 0 1118 1446
Size: 1118 328
BsmBI CGTCTCn'nnnn_
Cuts at: 0 680 1446
Size: 680 766
Bsp1286I G_dGCh'C
Cuts at: 0 19 1446
Size: 19 1427
BspHI T'CATG_A
Cuts at: 0 578 1446
Size: 578 868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 884 1446
Size: 884 562
BsrBI CCG'CTC
Cuts at: 0 681 1446
Size: 681 765
BsrFI r'CCGG_y
Cuts at: 0 726 1446
Size: 726 720
BsrGI T'GTAC_A
Cuts at: 0 512 1446
Size: 512 934
BstXI CCAn_nnnn'nTGG
Cuts at: 0 744 852 1446
Size: 744 108 594
Fragments arranged by size:
744 594 108
BstYI r'GATC_y
Cuts at: 0 968 1446
Size: 968 478
Bsu36I CC'TnA_GG
Cuts at: 0 1154 1169 1446
Size: 1154 15 277
Fragments arranged by size:
1154 277 15
BtgI C'CryG_G
Cuts at: 0 534 566 1446
Size: 534 32 880
Fragments arranged by size:
880 534 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 221 375 1196 1446
Size: 221 154 821 250
Fragments arranged by size:
821 250 221 154
EaeI y'GGCC_r
Cuts at: 0 473 1446
Size: 473 973
EarI CTCTTCn'nnn_
Cuts at: 0 545 1247 1265 1305 1376 1446
Size: 545 702 18 40 71 70
Fragments arranged by size:
702 545 71 70 40 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1289 1446
Size: 1096 193 157
Fragments arranged by size:
1096 193 157
EcoO109I rG'GnC_Cy
Cuts at: 0 411 627 1446
Size: 411 216 819
Fragments arranged by size:
819 411 216
EcoRV GAT'ATC
Cuts at: 0 465 639 1446
Size: 465 174 807
Fragments arranged by size:
807 465 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 688 720 781 813 1446
Size: 235 32 421 32 61 32 633
Fragments arranged by size:
633 421 235 61 32 32 32
HincII GTy'rAC
Cuts at: 0 672 1446
Size: 672 774
HpaI GTT'AAC
Cuts at: 0 672 1446
Size: 672 774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 233 1328 1446
Size: 36 197 1095 118
Fragments arranged by size:
1095 197 118 36
MscI TGG'CCA
Cuts at: 0 475 1446
Size: 475 971
MslI CAynn'nnrTG
Cuts at: 0 206 394 536 583 599 1446
Size: 206 188 142 47 16 847
Fragments arranged by size:
847 206 188 142 47 16
MspA1I CmG'CkG
Cuts at: 0 949 1446
Size: 949 497
NcoI C'CATG_G
Cuts at: 0 534 1446
Size: 534 912
NdeI CA'TA_TG
Cuts at: 0 939 1446
Size: 939 507
NspI r_CATG'y
Cuts at: 0 252 1446
Size: 252 1194
PciI A'CATG_T
Cuts at: 0 248 1446
Size: 248 1198
PflMI CCAn_nnn'nTGG
Cuts at: 0 622 1446
Size: 622 824
PfoI T'CCnGG_A
Cuts at: 0 479 1446
Size: 479 967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1240 1272 1446
Size: 1240 32 174
Fragments arranged by size:
1240 174 32
PpuMI rG'GwC_Cy
Cuts at: 0 627 1446
Size: 627 819
PvuII CAG'CTG
Cuts at: 0 949 1446
Size: 949 497
RsrII CG'GwC_CG
Cuts at: 0 570 1446
Size: 570 876
ScaI AGT'ACT
Cuts at: 0 610 1446
Size: 610 836
StuI AGG'CCT
Cuts at: 0 556 1446
Size: 556 890
StyI C'CwwG_G
Cuts at: 0 534 1394 1446
Size: 534 860 52
Fragments arranged by size:
860 534 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 98 1446
Size: 98 1348
TatI w'GTAC_w
Cuts at: 0 257 512 608 1446
Size: 257 255 96 838
Fragments arranged by size:
838 257 255 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1118 1150 1446
Size: 1118 32 296
Fragments arranged by size:
1118 296 32
Tth111I GACn'n_nGTC
Cuts at: 0 574 585 1446
Size: 574 11 861
Fragments arranged by size:
861 574 11
Enzymes that do cut:
AccI AclI AcuI AflIII AleI AlfI AlwNI ApoI
BanI BbsI BbvCI BciVI Bme1580I BmrI BpmI Bpu10I
BsaI BsaHI BsaXI BseRI BseYI BsgI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BstXI BstYI
Bsu36I BtgI BtgZI EaeI EarI Eco57MI EcoO109I EcoRV
Hin4I HincII HpaI MmeI MscI MslI MspA1I NcoI
NdeI NspI PciI PflMI PfoI PpiI PpuMI PvuII
RsrII ScaI StuI StyI TaqII TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanII BbeI BcgI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaWI
BsiEI BsiHKAI BsiWI BspEI BsrDI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI FalI FseI
FspI FspAI HaeII HindIII KasI KpnI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI SacI SacII SalI SanDI SapI SbfI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI
XhoI XmaI XmnI ZraI