Restriction map for
pF1KB7750
(Linear) (Six-base) MAPSORT of: KIBB7750.seq Check: 4565 from: 1 to: 1404
KIBB7750.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 238 339 1404
Size: 88 150 101 1065
Fragments arranged by size:
1065 150 101 88
AflIII A'CryG_T
Cuts at: 0 636 638 1404
Size: 636 2 766
Fragments arranged by size:
766 636 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 229 1404
Size: 229 1175
AlwNI CAG_nnn'CTG
Cuts at: 0 267 1404
Size: 267 1137
AvaI C'yCGr_G
Cuts at: 0 1270 1404
Size: 1270 134
BamHI G'GATC_C
Cuts at: 0 1328 1404
Size: 1328 76
BanII G_rGCy'C
Cuts at: 0 735 1102 1148 1404
Size: 735 367 46 256
Fragments arranged by size:
735 367 256 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 116 1404
Size: 116 1288
BbvCI CC'TCA_GC
Cuts at: 0 1014 1404
Size: 1014 390
BglI GCCn_nnn'nGGC
Cuts at: 0 1058 1404
Size: 1058 346
BmrI ACTGGGnnnn_n'
Cuts at: 0 14 476 901 1404
Size: 14 462 425 503
Fragments arranged by size:
503 462 425 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 754 1404
Size: 754 650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 734 1404
Size: 39 695 670
Fragments arranged by size:
695 670 39
Bpu10I CC'TnA_GC
Cuts at: 0 39 781 1014 1404
Size: 39 742 233 390
Fragments arranged by size:
742 390 233 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 914 1404
Size: 914 490
BsaAI yAC'GTr
Cuts at: 0 639 1404
Size: 639 765
BsaWI w'CCGG_w
Cuts at: 0 1085 1331 1404
Size: 1085 246 73
Fragments arranged by size:
1085 246 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 528 558 761 791 982 1012 1404
Size: 528 30 203 30 191 30 392
Fragments arranged by size:
528 392 203 191 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 549 990 993 996 999 1002 1300 1404
Size: 549 441 3 3 3 3 298 104
Fragments arranged by size:
549 441 298 104 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1102 1181 1225 1404
Size: 1102 79 44 179
Fragments arranged by size:
1102 179 79 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 1300 1404
Size: 181 1119 104
Fragments arranged by size:
1119 181 104
BsiEI CG_ry'CG
Cuts at: 0 811 1404
Size: 811 593
BsiHKAI G_wGCw'C
Cuts at: 0 976 1404
Size: 976 428
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1245 1404
Size: 1245 159
Bsp1286I G_dGCh'C
Cuts at: 0 735 976 1102 1148 1404
Size: 735 241 126 46 256
Fragments arranged by size:
735 256 241 126 46
BspEI T'CCGG_A
Cuts at: 0 1085 1404
Size: 1085 319
BssSI C'ACGA_G
Cuts at: 0 199 1093 1404
Size: 199 894 311
Fragments arranged by size:
894 311 199
BstXI CCAn_nnnn'nTGG
Cuts at: 0 698 1404
Size: 698 706
BstYI r'GATC_y
Cuts at: 0 428 1328 1404
Size: 428 900 76
Fragments arranged by size:
900 428 76
Bsu36I CC'TnA_GG
Cuts at: 0 241 665 1404
Size: 241 424 739
Fragments arranged by size:
739 424 241
BtgI C'CryG_G
Cuts at: 0 161 1404
Size: 161 1243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1292 1404
Size: 1292 112
BtsI GCAGTG_nn'
Cuts at: 0 954 1234 1404
Size: 954 280 170
Fragments arranged by size:
954 280 170
EaeI y'GGCC_r
Cuts at: 0 374 700 808 842 1404
Size: 374 326 108 34 562
Fragments arranged by size:
562 374 326 108 34
EagI C'GGCC_G
Cuts at: 0 808 1404
Size: 808 596
EarI CTCTTCn'nnn_
Cuts at: 0 146 167 214 1109 1404
Size: 146 21 47 895 295
Fragments arranged by size:
895 295 146 47 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 88 238 339 734 1404
Size: 39 49 150 101 395 670
Fragments arranged by size:
670 395 150 101 49 39
EcoO109I rG'GnC_Cy
Cuts at: 0 99 264 777 1221 1307 1404
Size: 99 165 513 444 86 97
Fragments arranged by size:
513 444 165 99 97 86
EcoRV GAT'ATC
Cuts at: 0 417 1404
Size: 417 987
HaeII r_GCGC'y
Cuts at: 0 402 1197 1404
Size: 402 795 207
Fragments arranged by size:
795 402 207
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 606 638 761 793 982 1014 1404
Size: 606 32 123 32 189 32 390
Fragments arranged by size:
606 390 189 123 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 986 1404
Size: 415 571 418
Fragments arranged by size:
571 418 415
MscI TGG'CCA
Cuts at: 0 376 1404
Size: 376 1028
MslI CAynn'nnrTG
Cuts at: 0 370 832 1404
Size: 370 462 572
Fragments arranged by size:
572 462 370
MspA1I CmG'CkG
Cuts at: 0 86 359 813 841 866 917 1169 1404
Size: 86 273 454 28 25 51 252 235
Fragments arranged by size:
454 273 252 235 86 51 28 25
NcoI C'CATG_G
Cuts at: 0 161 1404
Size: 161 1243
NotI GC'GGCC_GC
Cuts at: 0 808 1404
Size: 808 596
NspI r_CATG'y
Cuts at: 0 309 1404
Size: 309 1095
PflMI CCAn_nnn'nTGG
Cuts at: 0 267 898 1404
Size: 267 631 506
Fragments arranged by size:
631 506 267
PfoI T'CCnGG_A
Cuts at: 0 260 750 1404
Size: 260 490 654
Fragments arranged by size:
654 490 260
PmlI CAC'GTG
Cuts at: 0 639 1404
Size: 639 765
PpuMI rG'GwC_Cy
Cuts at: 0 264 777 1221 1307 1404
Size: 264 513 444 86 97
Fragments arranged by size:
513 444 264 97 86
PvuII CAG'CTG
Cuts at: 0 359 866 1404
Size: 359 507 538
Fragments arranged by size:
538 507 359
SanDI GG'GwC_CC
Cuts at: 0 1307 1404
Size: 1307 97
SapI GCTCTTCn'nnn_
Cuts at: 0 167 214 1404
Size: 167 47 1190
Fragments arranged by size:
1190 167 47
SfcI C'TryA_G
Cuts at: 0 942 1404
Size: 942 462
StyI C'CwwG_G
Cuts at: 0 95 161 926 1043 1148 1208 1404
Size: 95 66 765 117 105 60 196
Fragments arranged by size:
765 196 117 105 95 66 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 225 458 641 647 708 1404
Size: 225 233 183 6 61 696
Fragments arranged by size:
696 233 225 183 61 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 672 704 870 902 1404
Size: 672 32 166 32 502
Fragments arranged by size:
672 502 166 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 65 1404
Size: 65 1339
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1155 1404
Size: 1155 249
XmnI GAAnn'nnTTC
Cuts at: 0 440 1404
Size: 440 964
Enzymes that do cut:
AcuI AflIII AlfI AlwNI AvaI BamHI BanII BbsI
BbvCI BglI BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BssSI BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EagI EarI Eco57MI EcoO109I EcoRV HaeII
Hin4I MmeI MscI MslI MspA1I NcoI NotI NspI
PflMI PfoI PmlI PpuMI PvuII SanDI SapI SfcI
StyI TaqII TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanI BbeI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmtI BpuEI BsaBI
BsaHI BsiWI BsmI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I ClaI CspCI
DraI DraIII DrdI EciI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NruI
NsiI PacI PasI PciI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI XbaI XhoI XmaI ZraI