Restriction map for
pF1KB7757
(Linear) (Six-base) MAPSORT of: KIBB7757.seq Check: 7140 from: 1 to: 1341
KIBB7757.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AflIII A'CryG_T
Cuts at: 0 458 509 1341
Size: 458 51 832
Fragments arranged by size:
832 458 51
AleI CACnn'nnGTG
Cuts at: 0 782 1225 1341
Size: 782 443 116
Fragments arranged by size:
782 443 116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 584 1341
Size: 584 757
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 286 318 1341
Size: 286 32 1023
Fragments arranged by size:
1023 286 32
ApaI G_GGCC'C
Cuts at: 0 391 596 1341
Size: 391 205 745
Fragments arranged by size:
745 391 205
ApaLI G'TGCA_C
Cuts at: 0 883 1063 1341
Size: 883 180 278
Fragments arranged by size:
883 278 180
ApoI r'AATT_y
Cuts at: 0 951 1341
Size: 951 390
AvaI C'yCGr_G
Cuts at: 0 246 430 501 694 1341
Size: 246 184 71 193 647
Fragments arranged by size:
647 246 193 184 71
BanI G'GyrC_C
Cuts at: 0 133 201 625 631 1341
Size: 133 68 424 6 710
Fragments arranged by size:
710 424 133 68 6
BanII G_rGCy'C
Cuts at: 0 391 596 674 695 1171 1341
Size: 391 205 78 21 476 170
Fragments arranged by size:
476 391 205 170 78 21
BbeI G_GCGC'C
Cuts at: 0 137 205 629 635 1341
Size: 137 68 424 6 706
Fragments arranged by size:
706 424 137 68 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 927 1341
Size: 927 414
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 430 464 669 703 918 952 1341
Size: 430 34 205 34 215 34 389
Fragments arranged by size:
430 389 215 205 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 257 395 1341
Size: 257 138 946
Fragments arranged by size:
946 257 138
Bme1580I G_kGCm'C
Cuts at: 0 391 596 887 1067 1341
Size: 391 205 291 180 274
Fragments arranged by size:
391 291 274 205 180
BmgBI CAC'GTC
Cuts at: 0 81 96 194 255 765 1341
Size: 81 15 98 61 510 576
Fragments arranged by size:
576 510 98 81 61 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 15 146 1115 1341
Size: 15 131 969 226
Fragments arranged by size:
969 226 131 15
BsaAI yAC'GTr
Cuts at: 0 780 1062 1341
Size: 780 282 279
Fragments arranged by size:
780 282 279
BsaHI Gr'CG_yC
Cuts at: 0 11 134 202 407 626 632 1341
Size: 11 123 68 205 219 6 709
Fragments arranged by size:
709 219 205 123 68 11 6
BsaWI w'CCGG_w
Cuts at: 0 522 1341
Size: 522 819
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 244 274 1141 1171 1192 1222 1341
Size: 244 30 867 30 21 30 119
Fragments arranged by size:
867 244 119 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 412 835 1149 1263 1266 1341
Size: 412 423 314 114 3 75
Fragments arranged by size:
423 412 314 114 75 3
BseYI C'CCAG_C
Cuts at: 0 27 1341
Size: 27 1314
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1341
Size: 565 776
BsiEI CG_ry'CG
Cuts at: 0 35 176 374 523 1044 1187 1341
Size: 35 141 198 149 521 143 154
Fragments arranged by size:
521 198 154 149 143 141 35
BsiHKAI G_wGCw'C
Cuts at: 0 563 674 779 887 1067 1341
Size: 563 111 105 108 180 274
Fragments arranged by size:
563 274 180 111 108 105
BsmBI CGTCTCn'nnnn_
Cuts at: 0 86 1341
Size: 86 1255
Bsp1286I G_dGCh'C
Cuts at: 0 391 563 596 674 695 779 887 1067
Size: 391 172 33 78 21 84 108 180
Cuts at: 1067 1171 1341
Size: 104 170
Fragments arranged by size:
391 180 172 170 108 104 84 78
33 21
BsrFI r'CCGG_y
Cuts at: 0 13 175 451 628 1019 1182 1297 1341
Size: 13 162 276 177 391 163 115 44
Fragments arranged by size:
391 276 177 163 162 115 44 13
BssHII G'CGCG_C
Cuts at: 0 940 1341
Size: 940 401
BssSI C'ACGA_G
Cuts at: 0 160 439 748 1068 1341
Size: 160 279 309 320 273
Fragments arranged by size:
320 309 279 273 160
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1097 1341
Size: 1097 244
BstEII G'GTnAC_C
Cuts at: 0 540 1341
Size: 540 801
BstYI r'GATC_y
Cuts at: 0 959 1341
Size: 959 382
BtgI C'CryG_G
Cuts at: 0 71 410 770 1341
Size: 71 339 360 571
Fragments arranged by size:
571 360 339 71
DraI TTT'AAA
Cuts at: 0 956 1341
Size: 956 385
EaeI y'GGCC_r
Cuts at: 0 83 173 177 371 396 453 534 611
Size: 83 90 4 194 25 57 81 77
Cuts at: 611 704 1184 1299 1341
Size: 93 480 115 42
Fragments arranged by size:
480 194 115 93 90 83 81 77
57 42 25 4
EagI C'GGCC_G
Cuts at: 0 173 371 1184 1341
Size: 173 198 813 157
Fragments arranged by size:
813 198 173 157
EarI CTCTTCn'nnn_
Cuts at: 0 430 1320 1341
Size: 430 890 21
Fragments arranged by size:
890 430 21
EcoICRI GAG'CTC
Cuts at: 0 672 1341
Size: 672 669
EcoO109I rG'GnC_Cy
Cuts at: 0 387 1341
Size: 387 954
FseI GG_CCGG'CC
Cuts at: 0 179 1341
Size: 179 1162
FspI TGC'GCA
Cuts at: 0 1132 1341
Size: 1132 209
HaeII r_GCGC'y
Cuts at: 0 137 205 629 635 1026 1341
Size: 137 68 424 6 391 315
Fragments arranged by size:
424 391 315 137 68 6
HincII GTy'rAC
Cuts at: 0 477 870 1341
Size: 477 393 471
Fragments arranged by size:
477 471 393
HpaI GTT'AAC
Cuts at: 0 870 1341
Size: 870 471
KasI G'GCGC_C
Cuts at: 0 133 201 625 631 1341
Size: 133 68 424 6 710
Fragments arranged by size:
710 424 133 68 6
MluI A'CGCG_T
Cuts at: 0 458 1341
Size: 458 883
MscI TGG'CCA
Cuts at: 0 706 1341
Size: 706 635
MslI CAynn'nnrTG
Cuts at: 0 601 782 880 1060 1225 1341
Size: 601 181 98 180 165 116
Fragments arranged by size:
601 181 180 165 116 98
MspA1I CmG'CkG
Cuts at: 0 73 412 579 702 1341
Size: 73 339 167 123 639
Fragments arranged by size:
639 339 167 123 73
NaeI GCC'GGC
Cuts at: 0 15 177 453 630 1184 1299 1341
Size: 15 162 276 177 554 115 42
Fragments arranged by size:
554 276 177 162 115 42 15
NarI GG'CG_CC
Cuts at: 0 134 202 626 632 1341
Size: 134 68 424 6 709
Fragments arranged by size:
709 424 134 68 6
NgoMIV G'CCGG_C
Cuts at: 0 13 175 451 628 1182 1297 1341
Size: 13 162 276 177 554 115 44
Fragments arranged by size:
554 276 177 162 115 44 13
NspI r_CATG'y
Cuts at: 0 513 651 1059 1341
Size: 513 138 408 282
Fragments arranged by size:
513 408 282 138
PasI CC'CwG_GG
Cuts at: 0 228 1341
Size: 228 1113
PciI A'CATG_T
Cuts at: 0 509 1341
Size: 509 832
PfoI T'CCnGG_A
Cuts at: 0 5 1341
Size: 5 1336
PmlI CAC'GTG
Cuts at: 0 1062 1341
Size: 1062 279
PspOMI G'GGCC_C
Cuts at: 0 387 592 1341
Size: 387 205 749
Fragments arranged by size:
749 387 205
PvuI CG_AT'CG
Cuts at: 0 35 1341
Size: 35 1306
PvuII CAG'CTG
Cuts at: 0 579 702 1341
Size: 579 123 639
Fragments arranged by size:
639 579 123
SacI G_AGCT'C
Cuts at: 0 674 1341
Size: 674 667
SacII CC_GC'GG
Cuts at: 0 74 413 1341
Size: 74 339 928
Fragments arranged by size:
928 339 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 395 1341
Size: 395 946
SfoI GGC'GCC
Cuts at: 0 135 203 627 633 1341
Size: 135 68 424 6 708
Fragments arranged by size:
708 424 135 68 6
SgrAI Cr'CCGG_yG
Cuts at: 0 628 1341
Size: 628 713
SmaI CCC'GGG
Cuts at: 0 248 432 1341
Size: 248 184 909
Fragments arranged by size:
909 248 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 244 276 1141 1173 1341
Size: 244 32 865 32 168
Fragments arranged by size:
865 244 168 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 861 1341
Size: 861 480
XmaI C'CCGG_G
Cuts at: 0 246 430 1341
Size: 246 184 911
Fragments arranged by size:
911 246 184
XmnI GAAnn'nnTTC
Cuts at: 0 991 1341
Size: 991 350
Enzymes that do cut:
AflIII AleI AlfI AloI ApaI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BcgI BglI Bme1580I BmgBI
BmrI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BsrFI BssHII BssSI BstAPI
BstEII BstYI BtgI DraI EaeI EagI EarI EcoICRI
EcoO109I FseI FspI HaeII HincII HpaI KasI MluI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PfoI PmlI PspOMI PvuI PvuII SacI SacII
SfiI SfoI SgrAI SmaI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlwNI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BstBI BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI
EciI Eco57MI EcoNI EcoRI EcoRV FalI FspAI Hin4I
HindIII KpnI MfeI MmeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI RsrII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TatI
Tth111I XbaI XhoI ZraI