Restriction map for
pF1KB7758
(Linear) (Six-base) MAPSORT of: KIBB7758.seq Check: 4923 from: 1 to: 1494
KIBB7758.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 991 1494
Size: 991 503
AatII G_ACGT'C
Cuts at: 0 764 1494
Size: 764 730
AccI GT'mk_AC
Cuts at: 0 20 1494
Size: 20 1474
AflIII A'CryG_T
Cuts at: 0 1243 1494
Size: 1243 251
AlwNI CAG_nnn'CTG
Cuts at: 0 101 450 1494
Size: 101 349 1044
Fragments arranged by size:
1044 349 101
ApoI r'AATT_y
Cuts at: 0 996 1494
Size: 996 498
BamHI G'GATC_C
Cuts at: 0 1100 1494
Size: 1100 394
BanII G_rGCy'C
Cuts at: 0 62 262 1150 1492 1494
Size: 62 200 888 342 2
Fragments arranged by size:
888 342 200 62 2
BbsI GAAGACnn'nnnn_
Cuts at: 0 1023 1494
Size: 1023 471
BclI T'GATC_A
Cuts at: 0 140 1494
Size: 140 1354
BglII A'GATC_T
Cuts at: 0 314 705 843 1494
Size: 314 391 138 651
Fragments arranged by size:
651 391 314 138
Bme1580I G_kGCm'C
Cuts at: 0 1483 1494
Size: 1483 11
BmgBI CAC'GTC
Cuts at: 0 906 1494
Size: 906 588
BmrI ACTGGGnnnn_n'
Cuts at: 0 906 1077 1230 1494
Size: 906 171 153 264
Fragments arranged by size:
906 264 171 153
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1287 1494
Size: 1287 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 295 634 694 1179 1494
Size: 76 219 339 60 485 315
Fragments arranged by size:
485 339 315 219 76 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 1494
Size: 132 1362
BsaHI Gr'CG_yC
Cuts at: 0 761 1494
Size: 761 733
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 604 1260 1263 1494
Size: 604 656 3 231
Fragments arranged by size:
656 604 231 3
BseYI C'CCAG_C
Cuts at: 0 347 1494
Size: 347 1147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 969 1494
Size: 969 525
BsiHKAI G_wGCw'C
Cuts at: 0 62 296 1150 1392 1492 1494
Size: 62 234 854 242 100 2
Fragments arranged by size:
854 242 234 100 62 2
Bsp1286I G_dGCh'C
Cuts at: 0 62 262 296 1150 1392 1483 1492 1494
Size: 62 200 34 854 242 91 9 2
Fragments arranged by size:
854 242 200 91 62 34 9 2
BspHI T'CATG_A
Cuts at: 0 764 1494
Size: 764 730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 49 423 991 1437 1494
Size: 49 374 568 446 57
Fragments arranged by size:
568 446 374 57 49
BstBI TT'CG_AA
Cuts at: 0 934 1494
Size: 934 560
BstEII G'GTnAC_C
Cuts at: 0 36 379 1494
Size: 36 343 1115
Fragments arranged by size:
1115 343 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 54 1494
Size: 54 1440
BstYI r'GATC_y
Cuts at: 0 314 705 843 1100 1494
Size: 314 391 138 257 394
Fragments arranged by size:
394 391 314 257 138
BtgI C'CryG_G
Cuts at: 0 297 1383 1494
Size: 297 1086 111
Fragments arranged by size:
1086 297 111
BtsI GCAGTG_nn'
Cuts at: 0 116 930 1494
Size: 116 814 564
Fragments arranged by size:
814 564 116
DrdI GACnn_nn'nnGTC
Cuts at: 0 667 1494
Size: 667 827
EaeI y'GGCC_r
Cuts at: 0 90 221 1494
Size: 90 131 1273
Fragments arranged by size:
1273 131 90
EarI CTCTTCn'nnn_
Cuts at: 0 271 1056 1138 1281 1494
Size: 271 785 82 143 213
Fragments arranged by size:
785 271 213 143 82
EcoICRI GAG'CTC
Cuts at: 0 60 1148 1490 1494
Size: 60 1088 342 4
Fragments arranged by size:
1088 342 60 4
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 295 634 694 1179 1494
Size: 76 219 339 60 485 315
Fragments arranged by size:
485 339 315 219 76 60
EcoRI G'AATT_C
Cuts at: 0 996 1494
Size: 996 498
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 57 89 900 932 1258 1290 1379 1411
Size: 57 32 811 32 326 32 89 32
Cuts at: 1411 1494
Size: 83
Fragments arranged by size:
811 326 89 83 57 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 1494
Size: 228 1266
MscI TGG'CCA
Cuts at: 0 92 223 1494
Size: 92 131 1271
Fragments arranged by size:
1271 131 92
MslI CAynn'nnrTG
Cuts at: 0 1474 1494
Size: 1474 20
MspA1I CmG'CkG
Cuts at: 0 164 666 714 1494
Size: 164 502 48 780
Fragments arranged by size:
780 502 164 48
NcoI C'CATG_G
Cuts at: 0 297 1494
Size: 297 1197
NspI r_CATG'y
Cuts at: 0 1247 1494
Size: 1247 247
PciI A'CATG_T
Cuts at: 0 1243 1494
Size: 1243 251
PshAI GACnn'nnGTC
Cuts at: 0 883 1494
Size: 883 611
PstI C_TGCA'G
Cuts at: 0 434 446 749 1494
Size: 434 12 303 745
Fragments arranged by size:
745 434 303 12
PvuII CAG'CTG
Cuts at: 0 164 666 1494
Size: 164 502 828
Fragments arranged by size:
828 502 164
SacI G_AGCT'C
Cuts at: 0 62 1150 1492 1494
Size: 62 1088 342 2
Fragments arranged by size:
1088 342 62 2
SapI GCTCTTCn'nnn_
Cuts at: 0 1138 1494
Size: 1138 356
ScaI AGT'ACT
Cuts at: 0 892 1494
Size: 892 602
SfcI C'TryA_G
Cuts at: 0 430 442 656 745 1494
Size: 430 12 214 89 749
Fragments arranged by size:
749 430 214 89 12
SmlI C'TyrA_G
Cuts at: 0 147 1494
Size: 147 1347
SspI AAT'ATT
Cuts at: 0 1045 1063 1494
Size: 1045 18 431
Fragments arranged by size:
1045 431 18
StyI C'CwwG_G
Cuts at: 0 297 375 1286 1450 1494
Size: 297 78 911 164 44
Fragments arranged by size:
911 297 164 78 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 888 1494
Size: 888 606
TatI w'GTAC_w
Cuts at: 0 890 1494
Size: 890 604
XmnI GAAnn'nnTTC
Cuts at: 0 556 1494
Size: 556 938
ZraI GAC'GTC
Cuts at: 0 762 1494
Size: 762 732
Enzymes that do cut:
AarI AatII AccI AflIII AlwNI ApoI BamHI BanII
BbsI BclI BglII Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BstBI BstEII BstXI BstYI BtgI BtsI DrdI
EaeI EarI EcoICRI Eco57MI EcoRI Hin4I MmeI MscI
MslI MspA1I NcoI NspI PciI PshAI PstI PvuII
SacI SapI ScaI SfcI SmlI SspI StyI TaqII
TatI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbvCI BcgI BciVI BfrBI
BglI BlpI BmtI Bpu10I BsaI BsaAI BsaBI BsaWI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI