Restriction map for
pF1KB7767
(Linear) (Six-base) MAPSORT of: KIBB7767.seq Check: 7789 from: 1 to: 1341
KIBB7767.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1047 1341
Size: 1047 294
AatII G_ACGT'C
Cuts at: 0 812 1341
Size: 812 529
Acc65I G'GTAC_C
Cuts at: 0 76 1341
Size: 76 1265
AlwNI CAG_nnn'CTG
Cuts at: 0 44 1257 1341
Size: 44 1213 84
Fragments arranged by size:
1213 84 44
ApaI G_GGCC'C
Cuts at: 0 115 509 928 1089 1311 1341
Size: 115 394 419 161 222 30
Fragments arranged by size:
419 394 222 161 115 30
ApaLI G'TGCA_C
Cuts at: 0 754 1341
Size: 754 587
ApoI r'AATT_y
Cuts at: 0 829 1152 1341
Size: 829 323 189
Fragments arranged by size:
829 323 189
AvaI C'yCGr_G
Cuts at: 0 11 659 796 1341
Size: 11 648 137 545
Fragments arranged by size:
648 545 137 11
BanI G'GyrC_C
Cuts at: 0 76 670 743 1341
Size: 76 594 73 598
Fragments arranged by size:
598 594 76 73
BanII G_rGCy'C
Cuts at: 0 115 139 509 602 928 1089 1311 1341
Size: 115 24 370 93 326 161 222 30
Fragments arranged by size:
370 326 222 161 115 93 30 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 283 1272 1341
Size: 283 989 69
Fragments arranged by size:
989 283 69
BglI GCCn_nnn'nGGC
Cuts at: 0 655 1341
Size: 655 686
BglII A'GATC_T
Cuts at: 0 818 1341
Size: 818 523
Bme1580I G_kGCm'C
Cuts at: 0 115 509 673 758 928 1089 1311 1341
Size: 115 394 164 85 170 161 222 30
Fragments arranged by size:
394 222 170 164 161 115 85 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 457 930 1341
Size: 457 473 411
Fragments arranged by size:
473 457 411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 1114 1210 1341
Size: 515 599 96 131
Fragments arranged by size:
599 515 131 96
Bpu10I CC'TnA_GC
Cuts at: 0 1181 1341
Size: 1181 160
BsaI GGTCTCn'nnnn_
Cuts at: 0 1293 1341
Size: 1293 48
BsaHI Gr'CG_yC
Cuts at: 0 809 1341
Size: 809 532
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 952 1341
Size: 85 867 389
Fragments arranged by size:
867 389 85
BseYI C'CCAG_C
Cuts at: 0 16 97 413 645 775 1088 1310 1341
Size: 16 81 316 232 130 313 222 31
Fragments arranged by size:
316 313 232 222 130 81 31 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1251 1341
Size: 1251 90
BsiEI CG_ry'CG
Cuts at: 0 192 1341
Size: 192 1149
BsiHKAI G_wGCw'C
Cuts at: 0 758 1341
Size: 758 583
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1069 1341
Size: 1069 272
Bsp1286I G_dGCh'C
Cuts at: 0 115 139 509 602 673 758 928 1089
Size: 115 24 370 93 71 85 170 161
Cuts at: 1089 1311 1341
Size: 222 30
Fragments arranged by size:
370 222 170 161 115 93 85 71
30 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1047 1341
Size: 1047 294
BsrBI CCG'CTC
Cuts at: 0 795 1341
Size: 795 546
BsrFI r'CCGG_y
Cuts at: 0 79 949 1341
Size: 79 870 392
Fragments arranged by size:
870 392 79
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1167 1341
Size: 1167 174
BstYI r'GATC_y
Cuts at: 0 499 818 1341
Size: 499 319 523
Fragments arranged by size:
523 499 319
Bsu36I CC'TnA_GG
Cuts at: 0 464 1341
Size: 464 877
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 623 1341
Size: 623 718
BtsI GCAGTG_nn'
Cuts at: 0 60 72 93 768 972 1341
Size: 60 12 21 675 204 369
Fragments arranged by size:
675 369 204 60 21 12
EaeI y'GGCC_r
Cuts at: 0 170 189 1341
Size: 170 19 1152
Fragments arranged by size:
1152 170 19
EagI C'GGCC_G
Cuts at: 0 189 1341
Size: 189 1152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 925 1341
Size: 925 416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 1114 1210 1341
Size: 515 599 96 131
Fragments arranged by size:
599 515 131 96
EcoO109I rG'GnC_Cy
Cuts at: 0 111 165 451 506 663 924 1307 1341
Size: 111 54 286 55 157 261 383 34
Fragments arranged by size:
383 286 261 157 111 55 54 34
HaeII r_GCGC'y
Cuts at: 0 790 1341
Size: 790 551
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 216 248 270 302 1341
Size: 216 32 22 32 1039
Fragments arranged by size:
1039 216 32 32 22
KpnI G_GTAC'C
Cuts at: 0 80 1341
Size: 80 1261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1341
Size: 214 1127
MscI TGG'CCA
Cuts at: 0 172 1341
Size: 172 1169
MspA1I CmG'CkG
Cuts at: 0 41 687 1092 1176 1341
Size: 41 646 405 84 165
Fragments arranged by size:
646 405 165 84 41
NotI GC'GGCC_GC
Cuts at: 0 189 1341
Size: 189 1152
PasI CC'CwG_GG
Cuts at: 0 589 1341
Size: 589 752
PflMI CCAn_nnn'nTGG
Cuts at: 0 318 393 1341
Size: 318 75 948
Fragments arranged by size:
948 318 75
PfoI T'CCnGG_A
Cuts at: 0 158 1205 1341
Size: 158 1047 136
Fragments arranged by size:
1047 158 136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1247 1279 1341
Size: 1247 32 62
Fragments arranged by size:
1247 62 32
PpuMI rG'GwC_Cy
Cuts at: 0 451 1341
Size: 451 890
PspOMI G'GGCC_C
Cuts at: 0 111 505 924 1085 1307 1341
Size: 111 394 419 161 222 34
Fragments arranged by size:
419 394 222 161 111 34
PstI C_TGCA'G
Cuts at: 0 440 1341
Size: 440 901
PvuII CAG'CTG
Cuts at: 0 687 1092 1176 1341
Size: 687 405 84 165
Fragments arranged by size:
687 405 165 84
SanDI GG'GwC_CC
Cuts at: 0 451 1341
Size: 451 890
SexAI A'CCwGG_T
Cuts at: 0 362 1145 1341
Size: 362 783 196
Fragments arranged by size:
783 362 196
SfcI C'TryA_G
Cuts at: 0 436 1341
Size: 436 905
SmaI CCC'GGG
Cuts at: 0 13 1341
Size: 13 1328
SmlI C'TyrA_G
Cuts at: 0 796 1341
Size: 796 545
StuI AGG'CCT
Cuts at: 0 469 1341
Size: 469 872
StyI C'CwwG_G
Cuts at: 0 371 1034 1341
Size: 371 663 307
Fragments arranged by size:
663 371 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1264 1341
Size: 1264 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 39 71 1341
Size: 39 32 1270
Fragments arranged by size:
1270 39 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 490 1227 1341
Size: 490 737 114
Fragments arranged by size:
737 490 114
XhoI C'TCGA_G
Cuts at: 0 796 1341
Size: 796 545
XmaI C'CCGG_G
Cuts at: 0 11 1341
Size: 11 1330
XmnI GAAnn'nnTTC
Cuts at: 0 535 1341
Size: 535 806
ZraI GAC'GTC
Cuts at: 0 810 1341
Size: 810 531
Enzymes that do cut:
AarI AatII Acc65I AlwNI ApaI ApaLI ApoI AvaI
BanI BanII BbsI BglI BglII Bme1580I BmrI BpmI
Bpu10I BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstYI Bsu36I
BtgZI BtsI EaeI EagI EciI Eco57MI EcoO109I HaeII
Hin4I KpnI MmeI MscI MspA1I NotI PasI PflMI
PfoI PpiI PpuMI PspOMI PstI PvuII SanDI SexAI
SfcI SmaI SmlI StuI StyI TaqII TstI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI
BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsaXI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I BtgI ClaI CspCI DraI
DraIII DrdI EarI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI MfeI
MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspI PacI PciI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI