Restriction map for
pF1KB7772
(Linear) (Six-base) MAPSORT of: KIBB7772.seq Check: 6072 from: 1 to: 1515
KIBB7772.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AatII G_ACGT'C
Cuts at: 0 1142 1515
Size: 1142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 845 1394 1515
Size: 845 549 121
Fragments arranged by size:
845 549 121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 625 1515
Size: 625 890
AlwNI CAG_nnn'CTG
Cuts at: 0 34 832 1095 1515
Size: 34 798 263 420
Fragments arranged by size:
798 420 263 34
ApaI G_GGCC'C
Cuts at: 0 238 1055 1515
Size: 238 817 460
Fragments arranged by size:
817 460 238
ApoI r'AATT_y
Cuts at: 0 18 1515
Size: 18 1497
AvaI C'yCGr_G
Cuts at: 0 142 1515
Size: 142 1373
BamHI G'GATC_C
Cuts at: 0 3 1515
Size: 3 1512
BanI G'GyrC_C
Cuts at: 0 673 1515
Size: 673 842
BanII G_rGCy'C
Cuts at: 0 238 691 929 1055 1515
Size: 238 453 238 126 460
Fragments arranged by size:
460 453 238 238 126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 186 220 1515
Size: 186 34 1295
Fragments arranged by size:
1295 186 34
BglI GCCn_nnn'nGGC
Cuts at: 0 31 213 695 1515
Size: 31 182 482 820
Fragments arranged by size:
820 482 182 31
BglII A'GATC_T
Cuts at: 0 156 377 1410 1515
Size: 156 221 1033 105
Fragments arranged by size:
1033 221 156 105
BlpI GC'TnA_GC
Cuts at: 0 438 1515
Size: 438 1077
Bme1580I G_kGCm'C
Cuts at: 0 238 1055 1329 1515
Size: 238 817 274 186
Fragments arranged by size:
817 274 238 186
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 204 1515
Size: 204 1311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1065 1117 1197 1247 1295 1456 1515
Size: 1065 52 80 50 48 161 59
Fragments arranged by size:
1065 161 80 59 52 50 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 1515
Size: 840 675
BsaI GGTCTCn'nnnn_
Cuts at: 0 973 1515
Size: 973 542
BsaBI GATnn'nnATC
Cuts at: 0 1409 1515
Size: 1409 106
BsaHI Gr'CG_yC
Cuts at: 0 1139 1232 1515
Size: 1139 93 283
Fragments arranged by size:
1139 283 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1012 1042 1515
Size: 1012 30 473
Fragments arranged by size:
1012 473 30
BseYI C'CCAG_C
Cuts at: 0 239 1041 1194 1270 1515
Size: 239 802 153 76 245
Fragments arranged by size:
802 245 239 153 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 463 1515
Size: 75 388 1052
Fragments arranged by size:
1052 388 75
BsiEI CG_ry'CG
Cuts at: 0 230 1515
Size: 230 1285
BsiHKAI G_wGCw'C
Cuts at: 0 1361 1515
Size: 1361 154
BsmI GAATG_Cn'
Cuts at: 0 300 1400 1515
Size: 300 1100 115
Fragments arranged by size:
1100 300 115
Bsp1286I G_dGCh'C
Cuts at: 0 238 691 929 1055 1329 1361 1515
Size: 238 453 238 126 274 32 154
Fragments arranged by size:
453 274 238 238 154 126 32
BspHI T'CATG_A
Cuts at: 0 1145 1515
Size: 1145 370
BsrBI CCG'CTC
Cuts at: 0 86 560 1515
Size: 86 474 955
Fragments arranged by size:
955 474 86
BsrFI r'CCGG_y
Cuts at: 0 225 514 1003 1515
Size: 225 289 489 512
Fragments arranged by size:
512 489 289 225
BstAPI GCAn_nnn'nTGC
Cuts at: 0 725 1515
Size: 725 790
BstBI TT'CG_AA
Cuts at: 0 65 1515
Size: 65 1450
BstEII G'GTnAC_C
Cuts at: 0 999 1515
Size: 999 516
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1221 1278 1344 1515
Size: 1221 57 66 171
Fragments arranged by size:
1221 171 66 57
BstYI r'GATC_y
Cuts at: 0 3 54 156 377 1158 1404 1410 1509
Size: 3 51 102 221 781 246 6 99
Cuts at: 1509 1515
Size: 6
Fragments arranged by size:
781 246 221 102 99 51 6 6
3
Bsu36I CC'TnA_GG
Cuts at: 0 545 1400 1515
Size: 545 855 115
Fragments arranged by size:
855 545 115
BtgI C'CryG_G
Cuts at: 0 590 1226 1515
Size: 590 636 289
Fragments arranged by size:
636 590 289
BtsI GCAGTG_nn'
Cuts at: 0 801 1515
Size: 801 714
EaeI y'GGCC_r
Cuts at: 0 227 696 1223 1515
Size: 227 469 527 292
Fragments arranged by size:
527 469 292 227
EagI C'GGCC_G
Cuts at: 0 227 1515
Size: 227 1288
EarI CTCTTCn'nnn_
Cuts at: 0 945 1190 1515
Size: 945 245 325
Fragments arranged by size:
945 325 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 451 859 1515
Size: 451 408 656
Fragments arranged by size:
656 451 408
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 845 1065 1117 1197 1247 1295 1394 1456
Size: 845 220 52 80 50 48 99 62
Cuts at: 1456 1515
Size: 59
Fragments arranged by size:
845 220 99 80 62 59 52 50
48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 548 1515
Size: 548 967
EcoO109I rG'GnC_Cy
Cuts at: 0 234 235 268 1051 1052 1101 1267 1515
Size: 234 1 33 783 1 49 166 248
Fragments arranged by size:
783 248 234 166 49 33 1 1
EcoRI G'AATT_C
Cuts at: 0 18 1515
Size: 18 1497
HaeII r_GCGC'y
Cuts at: 0 189 350 1515
Size: 189 161 1165
Fragments arranged by size:
1165 189 161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 101 133 1515
Size: 101 32 1382
Fragments arranged by size:
1382 101 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1204 1515
Size: 706 498 311
Fragments arranged by size:
706 498 311
MscI TGG'CCA
Cuts at: 0 1225 1515
Size: 1225 290
NaeI GCC'GGC
Cuts at: 0 227 1515
Size: 227 1288
NcoI C'CATG_G
Cuts at: 0 590 1515
Size: 590 925
NdeI CA'TA_TG
Cuts at: 0 315 1515
Size: 315 1200
NgoMIV G'CCGG_C
Cuts at: 0 225 1515
Size: 225 1290
PflMI CCAn_nnn'nTGG
Cuts at: 0 1095 1515
Size: 1095 420
PpuMI rG'GwC_Cy
Cuts at: 0 268 1515
Size: 268 1247
PspOMI G'GGCC_C
Cuts at: 0 234 1051 1515
Size: 234 817 464
Fragments arranged by size:
817 464 234
SfcI C'TryA_G
Cuts at: 0 1020 1515
Size: 1020 495
SmlI C'TyrA_G
Cuts at: 0 819 1515
Size: 819 696
StyI C'CwwG_G
Cuts at: 0 590 1337 1515
Size: 590 747 178
Fragments arranged by size:
747 590 178
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 457 489 753 785 1515
Size: 457 32 264 32 730
Fragments arranged by size:
730 457 264 32 32
XbaI T'CTAG_A
Cuts at: 0 909 1407 1515
Size: 909 498 108
Fragments arranged by size:
909 498 108
XmnI GAAnn'nnTTC
Cuts at: 0 780 1515
Size: 780 735
ZraI GAC'GTC
Cuts at: 0 1140 1515
Size: 1140 375
Enzymes that do cut:
AatII AcuI AlfI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BcgI BglI BglII BlpI Bme1580I BplI
BpmI BpuEI BsaI BsaBI BsaHI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrFI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I MmeI MscI NaeI NcoI NdeI NgoMIV PflMI
PpuMI PspOMI SfcI SmlI StyI TstI XbaI XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BbvCI BciVI BclI BfrBI BmgBI
BmrI BmtI Bpu10I BsaAI BsaWI BseRI BsiWI BsmBI
BspEI BspMI BsrDI BsrGI BssHII BssSI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MslI MspA1I NarI NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI Tth111I
XcmI XhoI XmaI