Restriction map for
pF1KB7774
(Linear) (Six-base) MAPSORT of: KIBB7774.seq Check: 527 from: 1 to: 1518
KIBB7774.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 1251 1518
Size: 579 672 267
Fragments arranged by size:
672 579 267
AleI CACnn'nnGTG
Cuts at: 0 309 974 1058 1518
Size: 309 665 84 460
Fragments arranged by size:
665 460 309 84
ApaLI G'TGCA_C
Cuts at: 0 1105 1518
Size: 1105 413
ApoI r'AATT_y
Cuts at: 0 965 1009 1133 1385 1518
Size: 965 44 124 252 133
Fragments arranged by size:
965 252 133 124 44
BamHI G'GATC_C
Cuts at: 0 1121 1518
Size: 1121 397
BfrBI ATG'CAT
Cuts at: 0 1221 1518
Size: 1221 297
BglII A'GATC_T
Cuts at: 0 61 1518
Size: 61 1457
Bme1580I G_kGCm'C
Cuts at: 0 1109 1518
Size: 1109 409
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 291 1518
Size: 291 1227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 743 1518
Size: 190 553 775
Fragments arranged by size:
775 553 190
BsaI GGTCTCn'nnnn_
Cuts at: 0 166 555 723 1395 1518
Size: 166 389 168 672 123
Fragments arranged by size:
672 389 168 166 123
BsaWI w'CCGG_w
Cuts at: 0 1295 1518
Size: 1295 223
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 257 280 485 1518
Size: 257 23 205 1033
Fragments arranged by size:
1033 257 205 23
BsiHKAI G_wGCw'C
Cuts at: 0 1109 1518
Size: 1109 409
BsmI GAATG_Cn'
Cuts at: 0 577 1221 1518
Size: 577 644 297
Fragments arranged by size:
644 577 297
Bsp1286I G_dGCh'C
Cuts at: 0 1109 1518
Size: 1109 409
BspHI T'CATG_A
Cuts at: 0 492 1518
Size: 492 1026
BsrDI GCAATG_nn'
Cuts at: 0 324 614 1518
Size: 324 290 904
Fragments arranged by size:
904 324 290
BsrGI T'GTAC_A
Cuts at: 0 1495 1518
Size: 1495 23
BstYI r'GATC_y
Cuts at: 0 61 295 1121 1518
Size: 61 234 826 397
Fragments arranged by size:
826 397 234 61
Bsu36I CC'TnA_GG
Cuts at: 0 276 343 1518
Size: 276 67 1175
Fragments arranged by size:
1175 276 67
BtgI C'CryG_G
Cuts at: 0 282 1518
Size: 282 1236
BtsI GCAGTG_nn'
Cuts at: 0 304 1518
Size: 304 1214
EaeI y'GGCC_r
Cuts at: 0 209 1518
Size: 209 1309
EarI CTCTTCn'nnn_
Cuts at: 0 242 1518
Size: 242 1276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 579 743 1251 1518
Size: 190 389 164 508 267
Fragments arranged by size:
508 389 267 190 164
EcoO109I rG'GnC_Cy
Cuts at: 0 647 1518
Size: 647 871
EcoRI G'AATT_C
Cuts at: 0 965 1133 1385 1518
Size: 965 168 252 133
Fragments arranged by size:
965 252 168 133
EcoRV GAT'ATC
Cuts at: 0 860 1518
Size: 860 658
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 596 628 1518
Size: 256 32 308 32 890
Fragments arranged by size:
890 308 256 32 32
HincII GTy'rAC
Cuts at: 0 1291 1518
Size: 1291 227
HindIII A'AGCT_T
Cuts at: 0 506 1430 1518
Size: 506 924 88
Fragments arranged by size:
924 506 88
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 1518
Size: 215 1303
MscI TGG'CCA
Cuts at: 0 211 1518
Size: 211 1307
MslI CAynn'nnrTG
Cuts at: 0 158 309 353 497 974 1058 1518
Size: 158 151 44 144 477 84 460
Fragments arranged by size:
477 460 158 151 144 84 44
NcoI C'CATG_G
Cuts at: 0 282 1518
Size: 282 1236
NsiI A_TGCA'T
Cuts at: 0 1223 1518
Size: 1223 295
PflMI CCAn_nnn'nTGG
Cuts at: 0 10 1518
Size: 10 1508
PfoI T'CCnGG_A
Cuts at: 0 45 1518
Size: 45 1473
SapI GCTCTTCn'nnn_
Cuts at: 0 242 1518
Size: 242 1276
SfcI C'TryA_G
Cuts at: 0 177 513 1518
Size: 177 336 1005
Fragments arranged by size:
1005 336 177
StyI C'CwwG_G
Cuts at: 0 282 1518
Size: 282 1236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 480 1518
Size: 480 1038
TatI w'GTAC_w
Cuts at: 0 742 1495 1518
Size: 742 753 23
Fragments arranged by size:
753 742 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 683 715 1518
Size: 683 32 803
Fragments arranged by size:
803 683 32
XmnI GAAnn'nnTTC
Cuts at: 0 424 928 1012 1096 1264 1432 1518
Size: 424 504 84 84 168 168 86
Fragments arranged by size:
504 424 168 168 86 84 84
Enzymes that do cut:
AcuI AleI ApaLI ApoI BamHI BfrBI BglII Bme1580I
BplI BpmI BsaI BsaWI BseRI BsiHKAI BsmI Bsp1286I
BspHI BsrDI BsrGI BstYI Bsu36I BtgI BtsI EaeI
EarI Eco57MI EcoO109I EcoRI EcoRV Hin4I HincII HindIII
MmeI MscI MslI NcoI NsiI PflMI PfoI SapI
SfcI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BlpI BmgBI BmrI
BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseYI
BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI