(Linear) (Six-base) MAPSORT of: KIBB7775.seq Check: 158 from: 1 to: 1359 KIBB7775.orf With 182 enzymes: SgfI * June 26, 2008 10:49 .. Acc65I G'GTAC_C Cuts at: 0 557 1359 Size: 557 802 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 215 1207 1359 Size: 215 992 152 Fragments arranged by size: 992 215 152 ApoI r'AATT_y Cuts at: 0 151 461 716 1359 Size: 151 310 255 643 Fragments arranged by size: 643 310 255 151 BamHI G'GATC_C Cuts at: 0 1203 1359 Size: 1203 156 BanI G'GyrC_C Cuts at: 0 26 557 1359 Size: 26 531 802 Fragments arranged by size: 802 531 26 BbeI G_GCGC'C Cuts at: 0 30 1359 Size: 30 1329 BbsI GAAGACnn'nnnn_ Cuts at: 0 918 1359 Size: 918 441 BciVI GTATCCnnnnn_n' Cuts at: 0 1069 1359 Size: 1069 290 BclI T'GATC_A Cuts at: 0 350 1359 Size: 350 1009 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 410 1359 Size: 410 949 Bpu10I CC'TnA_GC Cuts at: 0 763 1359 Size: 763 596 BsaBI GATnn'nnATC Cuts at: 0 860 1359 Size: 860 499 BsaHI Gr'CG_yC Cuts at: 0 27 37 1359 Size: 27 10 1322 Fragments arranged by size: 1322 27 10 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1241 1271 1359 Size: 1241 30 88 Fragments arranged by size: 1241 88 30 BseYI C'CCAG_C Cuts at: 0 547 1359 Size: 547 812 BspHI T'CATG_A Cuts at: 0 491 1359 Size: 491 868 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1035 1359 Size: 1035 324 BsrGI T'GTAC_A Cuts at: 0 658 1359 Size: 658 701 BstYI r'GATC_y Cuts at: 0 690 1146 1203 1359 Size: 690 456 57 156 Fragments arranged by size: 690 456 156 57 BtgI C'CryG_G Cuts at: 0 1137 1359 Size: 1137 222 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 361 1359 Size: 361 998 ClaI AT'CG_AT Cuts at: 0 1274 1359 Size: 1274 85 EaeI y'GGCC_r Cuts at: 0 837 1359 Size: 837 522 EarI CTCTTCn'nnn_ Cuts at: 0 1226 1359 Size: 1226 133 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 215 410 1207 1359 Size: 215 195 797 152 Fragments arranged by size: 797 215 195 152 EcoRI G'AATT_C Cuts at: 0 151 1359 Size: 151 1208 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 34 523 696 1005 1359 Size: 34 489 173 309 354 Fragments arranged by size: 489 354 309 173 34 HaeII r_GCGC'y Cuts at: 0 30 1359 Size: 30 1329 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 763 795 1359 Size: 763 32 564 Fragments arranged by size: 763 564 32 KasI G'GCGC_C Cuts at: 0 26 1359 Size: 26 1333 KpnI G_GTAC'C Cuts at: 0 561 1359 Size: 561 798 MscI TGG'CCA Cuts at: 0 839 1359 Size: 839 520 NarI GG'CG_CC Cuts at: 0 27 1359 Size: 27 1332 NcoI C'CATG_G Cuts at: 0 1137 1359 Size: 1137 222 NdeI CA'TA_TG Cuts at: 0 453 1359 Size: 453 906 NspI r_CATG'y Cuts at: 0 737 876 1359 Size: 737 139 483 Fragments arranged by size: 737 483 139 PfoI T'CCnGG_A Cuts at: 0 944 1359 Size: 944 415 PshAI GACnn'nnGTC Cuts at: 0 922 1359 Size: 922 437 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1060 1092 1359 Size: 1060 32 267 Fragments arranged by size: 1060 267 32 PstI C_TGCA'G Cuts at: 0 1032 1344 1359 Size: 1032 312 15 Fragments arranged by size: 1032 312 15 ScaI AGT'ACT Cuts at: 0 844 1359 Size: 844 515 SfcI C'TryA_G Cuts at: 0 211 1028 1340 1359 Size: 211 817 312 19 Fragments arranged by size: 817 312 211 19 SfoI GGC'GCC Cuts at: 0 28 1359 Size: 28 1331 SphI G_CATG'C Cuts at: 0 737 876 1359 Size: 737 139 483 Fragments arranged by size: 737 483 139 SspI AAT'ATT Cuts at: 0 112 1359 Size: 112 1247 StyI C'CwwG_G Cuts at: 0 782 1137 1359 Size: 782 355 222 Fragments arranged by size: 782 355 222 TatI w'GTAC_w Cuts at: 0 658 842 1359 Size: 658 184 517 Fragments arranged by size: 658 517 184 Tth111I GACn'n_nGTC Cuts at: 0 997 1359 Size: 997 362 XmnI GAAnn'nnTTC Cuts at: 0 17 462 676 1285 1359 Size: 17 445 214 609 74 Fragments arranged by size: 609 445 214 74 17 Enzymes that do cut: Acc65I AcuI ApoI BamHI BanI BbeI BbsI BciVI BclI BpmI Bpu10I BsaBI BsaHI BsaXI BseYI BspHI BspMI BsrGI BstYI BtgI BtgZI ClaI EaeI EarI Eco57MI EcoRI FalI HaeII Hin4I KasI KpnI MscI NarI NcoI NdeI NspI PfoI PshAI PsrI PstI ScaI SfcI SfoI SphI SspI StyI TatI Tth111I XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanII BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtsI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HincII HindIII HpaI MfeI MluI MmeI MslI MspA1I NaeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI SwaI TaqII TstI XbaI XcmI XhoI XmaI ZraI