(Linear) (Six-base) MAPSORT of: KIBB7777.seq  Check: 5616  from: 1  to: 1359



KIBB7777.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AatII G_ACGT'C

Cuts at:      0     945    1359 
   Size:        945     414
 
AccI GT'mk_AC

Cuts at:      0     555    1065    1359 
   Size:        555     510     294

  Fragments arranged by size: 

                 555     510     294
 
AflIII A'CryG_T

Cuts at:      0    1049    1359 
   Size:       1049     310
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1094    1359 
   Size:       1094     265
 
AlwNI CAG_nnn'CTG

Cuts at:      0     238    1238    1359 
   Size:        238    1000     121

  Fragments arranged by size: 

                1000     238     121
 
ApoI r'AATT_y

Cuts at:      0     209     871    1359 
   Size:        209     662     488

  Fragments arranged by size: 

                 662     488     209
 
AvaI C'yCGr_G

Cuts at:      0     605    1359 
   Size:        605     754
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     412     763     913    1358    1359 
   Size:        412     351     150     445       1

  Fragments arranged by size: 

                 445     412     351     150       1
 
BbvCI CC'TCA_GC

Cuts at:      0     741    1359 
   Size:        741     618
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      53     678     718    1359 
   Size:         53     625      40     641

  Fragments arranged by size: 

                 641     625      53      40
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     618    1359 
   Size:        618     741
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1345    1359 
   Size:       1345      14
 
Bpu10I CC'TnA_GC

Cuts at:      0     741    1359 
   Size:        741     618
 
BsaAI yAC'GTr

Cuts at:      0    1052    1359 
   Size:       1052     307
 
BsaHI Gr'CG_yC

Cuts at:      0     942    1359 
   Size:        942     417
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     136    1359 
   Size:        136    1223
 
BsiEI CG_ry'CG

Cuts at:      0    1257    1359 
   Size:       1257     102
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     386    1359 
   Size:        386     973
 
BsrDI GCAATG_nn'

Cuts at:      0    1180    1359 
   Size:       1180     179
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     453    1359 
   Size:        453     906
 
BstYI r'GATC_y

Cuts at:      0      23    1220    1359 
   Size:         23    1197     139

  Fragments arranged by size: 

                1197     139      23
 
BtgI C'CryG_G

Cuts at:      0     101    1359 
   Size:        101    1258
 
EaeI y'GGCC_r

Cuts at:      0     455     964    1254    1286    1359 
   Size:        455     509     290      32      73

  Fragments arranged by size: 

                 509     455     290      73      32
 
EagI C'GGCC_G

Cuts at:      0    1254    1359 
   Size:       1254     105
 
EarI CTCTTCn'nnn_

Cuts at:      0      65     191     347     875     980    1359 
   Size:         65     126     156     528     105     379

  Fragments arranged by size: 

                 528     379     156     126     105      65
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     709    1359 
   Size:        709     650
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1345    1359 
   Size:       1345      14
 
EcoRV GAT'ATC

Cuts at:      0     978    1359 
   Size:        978     381
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     694    1359 
   Size:        694     665
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     214     246     568     600    1010    1042    1080    1112 
   Size:        214      32     322      32     410      32      38      32

Cuts at:   1112    1159    1191    1359 
   Size:         47      32     168

  Fragments arranged by size: 

                 410     322     214     168      47      38      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1066    1359 
   Size:       1066     293
 
MfeI C'AATT_G

Cuts at:      0     911    1227    1359 
   Size:        911     316     132

  Fragments arranged by size: 

                 911     316     132
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1078    1222    1359 
   Size:       1078     144     137

  Fragments arranged by size: 

                1078     144     137
 
MscI TGG'CCA

Cuts at:      0     457    1288    1359 
   Size:        457     831      71

  Fragments arranged by size: 

                 831     457      71
 
MspA1I CmG'CkG

Cuts at:      0    1259    1359 
   Size:       1259     100
 
NcoI C'CATG_G

Cuts at:      0     101    1359 
   Size:        101    1258
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     690    1359 
   Size:        690     669
 
PmlI CAC'GTG

Cuts at:      0    1052    1359 
   Size:       1052     307
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      80     112    1359 
   Size:         80      32    1247

  Fragments arranged by size: 

                1247      80      32
 
SalI G'TCGA_C

Cuts at:      0    1064    1359 
   Size:       1064     295
 
SapI GCTCTTCn'nnn_

Cuts at:      0     191    1359 
   Size:        191    1168
 
SfcI C'TryA_G

Cuts at:      0     552    1359 
   Size:        552     807
 
StyI C'CwwG_G

Cuts at:      0     101    1359 
   Size:        101    1258
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1188    1359 
   Size:       1188     171
 
TatI w'GTAC_w

Cuts at:      0     932    1359 
   Size:        932     427
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1186    1218    1359 
   Size:       1186      32     141

  Fragments arranged by size: 

                1186     141      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     941    1359 
   Size:        941     418
 
XbaI T'CTAG_A

Cuts at:      0     400     843    1359 
   Size:        400     443     516

  Fragments arranged by size: 

                 516     443     400
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     450    1111    1359 
   Size:        450     661     248

  Fragments arranged by size: 

                 661     450     248
 
ZraI GAC'GTC

Cuts at:      0     943    1359 
   Size:        943     416
 

 Enzymes that do cut:

    AatII     AccI   AflIII     AhdI    AlwNI     ApoI     AvaI     BbsI
    BbvCI     BmrI     BplI     BpmI   Bpu10I    BsaAI    BsaHI    BseRI
    BsiEI    BspMI    BsrDI    BstXI    BstYI     BtgI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoRV     FalI    Hin4I   HincII     MfeI
     MmeI     MscI   MspA1I     NcoI    PflMI     PmlI     PpiI     SalI
     SapI     SfcI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI    BpuEI
     BsaI    BsaBI    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MluI     MslI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     XhoI
     XmaI     XmnI