(Linear) (Six-base) MAPSORT of: KIBB7782.seq Check: 8657 from: 1 to: 1530 KIBB7782.orf With 182 enzymes: SgfI * June 26, 2008 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1040 1530 Size: 1040 490 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 121 457 460 883 1530 Size: 43 78 336 3 423 647 Fragments arranged by size: 647 423 336 78 43 3 AgeI A'CCGG_T Cuts at: 0 952 1530 Size: 952 578 AleI CACnn'nnGTG Cuts at: 0 955 1530 Size: 955 575 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1038 1530 Size: 1038 492 AlwNI CAG_nnn'CTG Cuts at: 0 659 861 1530 Size: 659 202 669 Fragments arranged by size: 669 659 202 ApaI G_GGCC'C Cuts at: 0 126 1276 1481 1530 Size: 126 1150 205 49 Fragments arranged by size: 1150 205 126 49 AvaI C'yCGr_G Cuts at: 0 669 694 1530 Size: 669 25 836 Fragments arranged by size: 836 669 25 BanI G'GyrC_C Cuts at: 0 1211 1255 1324 1384 1518 1530 Size: 1211 44 69 60 134 12 Fragments arranged by size: 1211 134 69 60 44 12 BanII G_rGCy'C Cuts at: 0 113 126 487 707 1145 1276 1295 1481 Size: 113 13 361 220 438 131 19 186 Cuts at: 1481 1530 Size: 49 Fragments arranged by size: 438 361 220 186 131 113 49 19 13 BbeI G_GCGC'C Cuts at: 0 1259 1388 1530 Size: 1259 129 142 Fragments arranged by size: 1259 142 129 BbsI GAAGACnn'nnnn_ Cuts at: 0 871 1044 1466 1530 Size: 871 173 422 64 Fragments arranged by size: 871 422 173 64 BglI GCCn_nnn'nGGC Cuts at: 0 234 711 1253 1358 1530 Size: 234 477 542 105 172 Fragments arranged by size: 542 477 234 172 105 Bme1580I G_kGCm'C Cuts at: 0 126 290 1007 1096 1216 1276 1329 1481 Size: 126 164 717 89 120 60 53 152 Cuts at: 1481 1530 Size: 49 Fragments arranged by size: 717 164 152 126 120 89 60 53 49 BmrI ACTGGGnnnn_n' Cuts at: 0 364 1530 Size: 364 1166 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 519 1530 Size: 112 407 1011 Fragments arranged by size: 1011 407 112 Bpu10I CC'TnA_GC Cuts at: 0 155 1159 1530 Size: 155 1004 371 Fragments arranged by size: 1004 371 155 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 1530 Size: 364 1166 BsaI GGTCTCn'nnnn_ Cuts at: 0 181 1012 1530 Size: 181 831 518 Fragments arranged by size: 831 518 181 BsaHI Gr'CG_yC Cuts at: 0 505 1046 1175 1256 1283 1385 1530 Size: 505 541 129 81 27 102 145 Fragments arranged by size: 541 505 145 129 102 81 27 BsaWI w'CCGG_w Cuts at: 0 527 952 1530 Size: 527 425 578 Fragments arranged by size: 578 527 425 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 78 108 472 502 604 634 667 697 Size: 78 30 364 30 102 30 33 30 Cuts at: 697 1530 Size: 833 Fragments arranged by size: 833 364 102 78 33 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1135 1450 1453 1456 1465 1468 1530 Size: 1135 315 3 3 9 3 62 Fragments arranged by size: 1135 315 62 9 3 3 3 BseYI C'CCAG_C Cuts at: 0 1269 1306 1530 Size: 1269 37 224 Fragments arranged by size: 1269 224 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 157 586 1530 Size: 157 429 944 Fragments arranged by size: 944 429 157 BsiEI CG_ry'CG Cuts at: 0 273 1530 Size: 273 1257 BsiHKAI G_wGCw'C Cuts at: 0 113 332 1530 Size: 113 219 1198 Fragments arranged by size: 1198 219 113 Bsp1286I G_dGCh'C Cuts at: 0 113 126 290 332 487 707 1007 1096 Size: 113 13 164 42 155 220 300 89 Cuts at: 1096 1145 1216 1276 1295 1329 1481 1530 Size: 49 71 60 19 34 152 49 Fragments arranged by size: 300 220 164 155 152 113 89 71 60 49 49 42 34 19 13 BspEI T'CCGG_A Cuts at: 0 527 1530 Size: 527 1003 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 558 1030 1040 1067 1530 Size: 558 472 10 27 463 Fragments arranged by size: 558 472 463 27 10 BsrBI CCG'CTC Cuts at: 0 1322 1530 Size: 1322 208 BsrFI r'CCGG_y Cuts at: 0 233 277 952 1521 1530 Size: 233 44 675 569 9 Fragments arranged by size: 675 569 233 44 9 BssSI C'ACGA_G Cuts at: 0 106 1206 1264 1530 Size: 106 1100 58 266 Fragments arranged by size: 1100 266 106 58 BstXI CCAn_nnnn'nTGG Cuts at: 0 30 1530 Size: 30 1500 BstYI r'GATC_y Cuts at: 0 1154 1530 Size: 1154 376 Bsu36I CC'TnA_GG Cuts at: 0 608 1530 Size: 608 922 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 618 1530 Size: 618 912 BtsI GCAGTG_nn' Cuts at: 0 1042 1530 Size: 1042 488 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 497 532 1530 Size: 497 35 998 Fragments arranged by size: 998 497 35 EaeI y'GGCC_r Cuts at: 0 758 1530 Size: 758 772 EarI CTCTTCn'nnn_ Cuts at: 0 1442 1466 1530 Size: 1442 24 64 Fragments arranged by size: 1442 64 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 22 382 1530 Size: 22 360 1148 Fragments arranged by size: 1148 360 22 EcoICRI GAG'CTC Cuts at: 0 111 1530 Size: 111 1419 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 112 121 457 460 519 883 1530 Size: 43 69 9 336 3 59 364 647 Fragments arranged by size: 647 364 336 69 59 43 9 3 EcoO109I rG'GnC_Cy Cuts at: 0 122 123 307 1273 1477 1478 1512 1530 Size: 122 1 184 966 204 1 34 18 Fragments arranged by size: 966 204 184 122 34 18 1 1 HaeII r_GCGC'y Cuts at: 0 284 1259 1388 1494 1530 Size: 284 975 129 106 36 Fragments arranged by size: 975 284 129 106 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1402 1434 1530 Size: 1402 32 96 Fragments arranged by size: 1402 96 32 HincII GTy'rAC Cuts at: 0 648 1314 1530 Size: 648 666 216 Fragments arranged by size: 666 648 216 HindIII A'AGCT_T Cuts at: 0 472 1530 Size: 472 1058 KasI G'GCGC_C Cuts at: 0 1255 1384 1530 Size: 1255 129 146 Fragments arranged by size: 1255 146 129 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 77 1530 Size: 77 1453 MslI CAynn'nnrTG Cuts at: 0 34 637 955 1087 1264 1530 Size: 34 603 318 132 177 266 Fragments arranged by size: 603 318 266 177 132 34 MspA1I CmG'CkG Cuts at: 0 132 300 602 834 981 1241 1496 1530 Size: 132 168 302 232 147 260 255 34 Fragments arranged by size: 302 260 255 232 168 147 132 34 NaeI GCC'GGC Cuts at: 0 279 1523 1530 Size: 279 1244 7 Fragments arranged by size: 1244 279 7 NarI GG'CG_CC Cuts at: 0 1256 1385 1530 Size: 1256 129 145 Fragments arranged by size: 1256 145 129 NgoMIV G'CCGG_C Cuts at: 0 277 1521 1530 Size: 277 1244 9 Fragments arranged by size: 1244 277 9 PasI CC'CwG_GG Cuts at: 0 1329 1530 Size: 1329 201 PspOMI G'GGCC_C Cuts at: 0 122 1272 1477 1530 Size: 122 1150 205 53 Fragments arranged by size: 1150 205 122 53 PstI C_TGCA'G Cuts at: 0 548 857 1037 1064 1246 1530 Size: 548 309 180 27 182 284 Fragments arranged by size: 548 309 284 182 180 27 PvuII CAG'CTG Cuts at: 0 132 602 834 1241 1530 Size: 132 470 232 407 289 Fragments arranged by size: 470 407 289 232 132 SacI G_AGCT'C Cuts at: 0 113 1530 Size: 113 1417 ScaI AGT'ACT Cuts at: 0 343 1530 Size: 343 1187 SexAI A'CCwGG_T Cuts at: 0 821 1193 1530 Size: 821 372 337 Fragments arranged by size: 821 372 337 SfcI C'TryA_G Cuts at: 0 147 544 853 1033 1060 1242 1530 Size: 147 397 309 180 27 182 288 Fragments arranged by size: 397 309 288 182 180 147 27 SfoI GGC'GCC Cuts at: 0 1257 1386 1530 Size: 1257 129 144 Fragments arranged by size: 1257 144 129 SgrAI Cr'CCGG_yG Cuts at: 0 952 1530 Size: 952 578 SmaI CCC'GGG Cuts at: 0 671 696 1530 Size: 671 25 834 Fragments arranged by size: 834 671 25 SmlI C'TyrA_G Cuts at: 0 379 1530 Size: 379 1151 SspI AAT'ATT Cuts at: 0 315 1530 Size: 315 1215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 751 1530 Size: 751 779 TatI w'GTAC_w Cuts at: 0 341 1530 Size: 341 1189 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 78 110 472 504 1192 1224 1530 Size: 78 32 362 32 688 32 306 Fragments arranged by size: 688 362 306 78 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1048 1530 Size: 1048 482 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 297 1266 1530 Size: 297 969 264 Fragments arranged by size: 969 297 264 XmaI C'CCGG_G Cuts at: 0 669 694 1530 Size: 669 25 836 Fragments arranged by size: 836 669 25 Enzymes that do cut: AarI AcuI AgeI AleI AlfI AlwNI ApaI AvaI BanI BanII BbeI BbsI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstXI BstYI Bsu36I BtgZI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII HindIII KasI MmeI MslI MspA1I NaeI NarI NgoMIV PasI PspOMI PstI PvuII SacI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI SspI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgI ClaI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SfiI SgfI SnaBI SpeI SphI SrfI StuI StyI SwaI XbaI XhoI XmnI ZraI