Restriction map for
pF1KB7782
(Linear) (Six-base) MAPSORT of: KIBB7782.seq Check: 8657 from: 1 to: 1530
KIBB7782.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1040 1530
Size: 1040 490
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 121 457 460 883 1530
Size: 43 78 336 3 423 647
Fragments arranged by size:
647 423 336 78 43 3
AgeI A'CCGG_T
Cuts at: 0 952 1530
Size: 952 578
AleI CACnn'nnGTG
Cuts at: 0 955 1530
Size: 955 575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1038 1530
Size: 1038 492
AlwNI CAG_nnn'CTG
Cuts at: 0 659 861 1530
Size: 659 202 669
Fragments arranged by size:
669 659 202
ApaI G_GGCC'C
Cuts at: 0 126 1276 1481 1530
Size: 126 1150 205 49
Fragments arranged by size:
1150 205 126 49
AvaI C'yCGr_G
Cuts at: 0 669 694 1530
Size: 669 25 836
Fragments arranged by size:
836 669 25
BanI G'GyrC_C
Cuts at: 0 1211 1255 1324 1384 1518 1530
Size: 1211 44 69 60 134 12
Fragments arranged by size:
1211 134 69 60 44 12
BanII G_rGCy'C
Cuts at: 0 113 126 487 707 1145 1276 1295 1481
Size: 113 13 361 220 438 131 19 186
Cuts at: 1481 1530
Size: 49
Fragments arranged by size:
438 361 220 186 131 113 49 19
13
BbeI G_GCGC'C
Cuts at: 0 1259 1388 1530
Size: 1259 129 142
Fragments arranged by size:
1259 142 129
BbsI GAAGACnn'nnnn_
Cuts at: 0 871 1044 1466 1530
Size: 871 173 422 64
Fragments arranged by size:
871 422 173 64
BglI GCCn_nnn'nGGC
Cuts at: 0 234 711 1253 1358 1530
Size: 234 477 542 105 172
Fragments arranged by size:
542 477 234 172 105
Bme1580I G_kGCm'C
Cuts at: 0 126 290 1007 1096 1216 1276 1329 1481
Size: 126 164 717 89 120 60 53 152
Cuts at: 1481 1530
Size: 49
Fragments arranged by size:
717 164 152 126 120 89 60 53
49
BmrI ACTGGGnnnn_n'
Cuts at: 0 364 1530
Size: 364 1166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 519 1530
Size: 112 407 1011
Fragments arranged by size:
1011 407 112
Bpu10I CC'TnA_GC
Cuts at: 0 155 1159 1530
Size: 155 1004 371
Fragments arranged by size:
1004 371 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1530
Size: 364 1166
BsaI GGTCTCn'nnnn_
Cuts at: 0 181 1012 1530
Size: 181 831 518
Fragments arranged by size:
831 518 181
BsaHI Gr'CG_yC
Cuts at: 0 505 1046 1175 1256 1283 1385 1530
Size: 505 541 129 81 27 102 145
Fragments arranged by size:
541 505 145 129 102 81 27
BsaWI w'CCGG_w
Cuts at: 0 527 952 1530
Size: 527 425 578
Fragments arranged by size:
578 527 425
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 78 108 472 502 604 634 667 697
Size: 78 30 364 30 102 30 33 30
Cuts at: 697 1530
Size: 833
Fragments arranged by size:
833 364 102 78 33 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1135 1450 1453 1456 1465 1468 1530
Size: 1135 315 3 3 9 3 62
Fragments arranged by size:
1135 315 62 9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1269 1306 1530
Size: 1269 37 224
Fragments arranged by size:
1269 224 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 586 1530
Size: 157 429 944
Fragments arranged by size:
944 429 157
BsiEI CG_ry'CG
Cuts at: 0 273 1530
Size: 273 1257
BsiHKAI G_wGCw'C
Cuts at: 0 113 332 1530
Size: 113 219 1198
Fragments arranged by size:
1198 219 113
Bsp1286I G_dGCh'C
Cuts at: 0 113 126 290 332 487 707 1007 1096
Size: 113 13 164 42 155 220 300 89
Cuts at: 1096 1145 1216 1276 1295 1329 1481 1530
Size: 49 71 60 19 34 152 49
Fragments arranged by size:
300 220 164 155 152 113 89 71
60 49 49 42 34 19 13
BspEI T'CCGG_A
Cuts at: 0 527 1530
Size: 527 1003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 558 1030 1040 1067 1530
Size: 558 472 10 27 463
Fragments arranged by size:
558 472 463 27 10
BsrBI CCG'CTC
Cuts at: 0 1322 1530
Size: 1322 208
BsrFI r'CCGG_y
Cuts at: 0 233 277 952 1521 1530
Size: 233 44 675 569 9
Fragments arranged by size:
675 569 233 44 9
BssSI C'ACGA_G
Cuts at: 0 106 1206 1264 1530
Size: 106 1100 58 266
Fragments arranged by size:
1100 266 106 58
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 1530
Size: 30 1500
BstYI r'GATC_y
Cuts at: 0 1154 1530
Size: 1154 376
Bsu36I CC'TnA_GG
Cuts at: 0 608 1530
Size: 608 922
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 618 1530
Size: 618 912
BtsI GCAGTG_nn'
Cuts at: 0 1042 1530
Size: 1042 488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 497 532 1530
Size: 497 35 998
Fragments arranged by size:
998 497 35
EaeI y'GGCC_r
Cuts at: 0 758 1530
Size: 758 772
EarI CTCTTCn'nnn_
Cuts at: 0 1442 1466 1530
Size: 1442 24 64
Fragments arranged by size:
1442 64 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 382 1530
Size: 22 360 1148
Fragments arranged by size:
1148 360 22
EcoICRI GAG'CTC
Cuts at: 0 111 1530
Size: 111 1419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 112 121 457 460 519 883 1530
Size: 43 69 9 336 3 59 364 647
Fragments arranged by size:
647 364 336 69 59 43 9 3
EcoO109I rG'GnC_Cy
Cuts at: 0 122 123 307 1273 1477 1478 1512 1530
Size: 122 1 184 966 204 1 34 18
Fragments arranged by size:
966 204 184 122 34 18 1 1
HaeII r_GCGC'y
Cuts at: 0 284 1259 1388 1494 1530
Size: 284 975 129 106 36
Fragments arranged by size:
975 284 129 106 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1402 1434 1530
Size: 1402 32 96
Fragments arranged by size:
1402 96 32
HincII GTy'rAC
Cuts at: 0 648 1314 1530
Size: 648 666 216
Fragments arranged by size:
666 648 216
HindIII A'AGCT_T
Cuts at: 0 472 1530
Size: 472 1058
KasI G'GCGC_C
Cuts at: 0 1255 1384 1530
Size: 1255 129 146
Fragments arranged by size:
1255 146 129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 1530
Size: 77 1453
MslI CAynn'nnrTG
Cuts at: 0 34 637 955 1087 1264 1530
Size: 34 603 318 132 177 266
Fragments arranged by size:
603 318 266 177 132 34
MspA1I CmG'CkG
Cuts at: 0 132 300 602 834 981 1241 1496 1530
Size: 132 168 302 232 147 260 255 34
Fragments arranged by size:
302 260 255 232 168 147 132 34
NaeI GCC'GGC
Cuts at: 0 279 1523 1530
Size: 279 1244 7
Fragments arranged by size:
1244 279 7
NarI GG'CG_CC
Cuts at: 0 1256 1385 1530
Size: 1256 129 145
Fragments arranged by size:
1256 145 129
NgoMIV G'CCGG_C
Cuts at: 0 277 1521 1530
Size: 277 1244 9
Fragments arranged by size:
1244 277 9
PasI CC'CwG_GG
Cuts at: 0 1329 1530
Size: 1329 201
PspOMI G'GGCC_C
Cuts at: 0 122 1272 1477 1530
Size: 122 1150 205 53
Fragments arranged by size:
1150 205 122 53
PstI C_TGCA'G
Cuts at: 0 548 857 1037 1064 1246 1530
Size: 548 309 180 27 182 284
Fragments arranged by size:
548 309 284 182 180 27
PvuII CAG'CTG
Cuts at: 0 132 602 834 1241 1530
Size: 132 470 232 407 289
Fragments arranged by size:
470 407 289 232 132
SacI G_AGCT'C
Cuts at: 0 113 1530
Size: 113 1417
ScaI AGT'ACT
Cuts at: 0 343 1530
Size: 343 1187
SexAI A'CCwGG_T
Cuts at: 0 821 1193 1530
Size: 821 372 337
Fragments arranged by size:
821 372 337
SfcI C'TryA_G
Cuts at: 0 147 544 853 1033 1060 1242 1530
Size: 147 397 309 180 27 182 288
Fragments arranged by size:
397 309 288 182 180 147 27
SfoI GGC'GCC
Cuts at: 0 1257 1386 1530
Size: 1257 129 144
Fragments arranged by size:
1257 144 129
SgrAI Cr'CCGG_yG
Cuts at: 0 952 1530
Size: 952 578
SmaI CCC'GGG
Cuts at: 0 671 696 1530
Size: 671 25 834
Fragments arranged by size:
834 671 25
SmlI C'TyrA_G
Cuts at: 0 379 1530
Size: 379 1151
SspI AAT'ATT
Cuts at: 0 315 1530
Size: 315 1215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 751 1530
Size: 751 779
TatI w'GTAC_w
Cuts at: 0 341 1530
Size: 341 1189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 78 110 472 504 1192 1224 1530
Size: 78 32 362 32 688 32 306
Fragments arranged by size:
688 362 306 78 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1048 1530
Size: 1048 482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 297 1266 1530
Size: 297 969 264
Fragments arranged by size:
969 297 264
XmaI C'CCGG_G
Cuts at: 0 669 694 1530
Size: 669 25 836
Fragments arranged by size:
836 669 25
Enzymes that do cut:
AarI AcuI AgeI AleI AlfI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BglI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI
BssSI BstXI BstYI Bsu36I BtgZI BtsI CspCI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII
HindIII KasI MmeI MslI MspA1I NaeI NarI NgoMIV
PasI PspOMI PstI PvuII SacI ScaI SexAI SfcI
SfoI SgrAI SmaI SmlI SspI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI BsaAI BsaBI BsiWI BsmI BsmBI
BspHI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
BtgI ClaI DraI DraIII DrdI EagI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HpaI KpnI MfeI
MluI MscI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SfiI SgfI SnaBI SpeI
SphI SrfI StuI StyI SwaI XbaI XhoI XmnI
ZraI