Restriction map for
pF1KB7786
(Linear) (Six-base) MAPSORT of: KIBB7786.seq Check: 9193 from: 1 to: 1536
KIBB7786.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1364 1472 1536
Size: 1364 108 64
Fragments arranged by size:
1364 108 64
AleI CACnn'nnGTG
Cuts at: 0 990 1536
Size: 990 546
AlwNI CAG_nnn'CTG
Cuts at: 0 598 866 1525 1528 1536
Size: 598 268 659 3 8
Fragments arranged by size:
659 598 268 8 3
ApaI G_GGCC'C
Cuts at: 0 279 1536
Size: 279 1257
ApaLI G'TGCA_C
Cuts at: 0 416 1379 1536
Size: 416 963 157
Fragments arranged by size:
963 416 157
ApoI r'AATT_y
Cuts at: 0 187 340 1536
Size: 187 153 1196
Fragments arranged by size:
1196 187 153
AvaI C'yCGr_G
Cuts at: 0 1457 1536
Size: 1457 79
BamHI G'GATC_C
Cuts at: 0 201 302 618 1164 1536
Size: 201 101 316 546 372
Fragments arranged by size:
546 372 316 201 101
BanI G'GyrC_C
Cuts at: 0 605 1258 1536
Size: 605 653 278
Fragments arranged by size:
653 605 278
BanII G_rGCy'C
Cuts at: 0 279 850 974 1376 1536
Size: 279 571 124 402 160
Fragments arranged by size:
571 402 279 160 124
BbeI G_GCGC'C
Cuts at: 0 609 1536
Size: 609 927
BbsI GAAGACnn'nnnn_
Cuts at: 0 1352 1536
Size: 1352 184
BfrBI ATG'CAT
Cuts at: 0 1002 1536
Size: 1002 534
BglI GCCn_nnn'nGGC
Cuts at: 0 227 1536
Size: 227 1309
BlpI GC'TnA_GC
Cuts at: 0 268 591 1465 1536
Size: 268 323 874 71
Fragments arranged by size:
874 323 268 71
Bme1580I G_kGCm'C
Cuts at: 0 279 420 1225 1307 1383 1536
Size: 279 141 805 82 76 153
Fragments arranged by size:
805 279 153 141 82 76
BmrI ACTGGGnnnn_n'
Cuts at: 0 72 1370 1536
Size: 72 1298 166
Fragments arranged by size:
1298 166 72
BmtI G_CTAG'C
Cuts at: 0 515 1536
Size: 515 1021
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 606 1536
Size: 606 930
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 601 615 1354 1536
Size: 145 456 14 739 182
Fragments arranged by size:
739 456 182 145 14
Bpu10I CC'TnA_GC
Cuts at: 0 844 1536
Size: 844 692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 1196 1536
Size: 896 300 340
Fragments arranged by size:
896 340 300
BsaHI Gr'CG_yC
Cuts at: 0 606 1536
Size: 606 930
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 459 489 1536
Size: 459 30 1047
Fragments arranged by size:
1047 459 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 665 1024 1102 1216 1536
Size: 665 359 78 114 320
Fragments arranged by size:
665 359 320 114 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 1265 1536
Size: 511 754 271
Fragments arranged by size:
754 511 271
BsiHKAI G_wGCw'C
Cuts at: 0 420 1208 1383 1536
Size: 420 788 175 153
Fragments arranged by size:
788 420 175 153
BsmI GAATG_Cn'
Cuts at: 0 642 1536
Size: 642 894
Bsp1286I G_dGCh'C
Cuts at: 0 279 420 850 974 1208 1225 1307 1376
Size: 279 141 430 124 234 17 82 69
Cuts at: 1376 1383 1536
Size: 7 153
Fragments arranged by size:
430 279 234 153 141 124 82 69
17 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1158 1536
Size: 1158 378
BsrFI r'CCGG_y
Cuts at: 0 7 1536
Size: 7 1529
BssSI C'ACGA_G
Cuts at: 0 1190 1536
Size: 1190 346
BstAPI GCAn_nnn'nTGC
Cuts at: 0 239 866 1536
Size: 239 627 670
Fragments arranged by size:
670 627 239
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1292 1536
Size: 1292 244
BstYI r'GATC_y
Cuts at: 0 101 201 302 618 1164 1536
Size: 101 100 101 316 546 372
Fragments arranged by size:
546 372 316 101 101 100
Bsu36I CC'TnA_GG
Cuts at: 0 884 1007 1177 1536
Size: 884 123 170 359
Fragments arranged by size:
884 359 170 123
BtgI C'CryG_G
Cuts at: 0 762 1115 1536
Size: 762 353 421
Fragments arranged by size:
762 421 353
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 144 1536
Size: 144 1392
BtsI GCAGTG_nn'
Cuts at: 0 985 997 1536
Size: 985 12 539
Fragments arranged by size:
985 539 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 383 418 1536
Size: 383 35 1118
Fragments arranged by size:
1118 383 35
EaeI y'GGCC_r
Cuts at: 0 531 660 759 1536
Size: 531 129 99 777
Fragments arranged by size:
777 531 129 99
EarI CTCTTCn'nnn_
Cuts at: 0 137 444 1211 1241 1536
Size: 137 307 767 30 295
Fragments arranged by size:
767 307 295 137 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 139 1071 1536
Size: 139 932 465
Fragments arranged by size:
932 465 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 601 615 1354 1364 1472 1536
Size: 145 456 14 739 10 108 64
Fragments arranged by size:
739 456 145 108 64 14 10
EcoO109I rG'GnC_Cy
Cuts at: 0 276 1501 1536
Size: 276 1225 35
Fragments arranged by size:
1225 276 35
HaeII r_GCGC'y
Cuts at: 0 609 1437 1536
Size: 609 828 99
Fragments arranged by size:
828 609 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 610 642 648 680 1536
Size: 610 32 6 32 856
Fragments arranged by size:
856 610 32 32 6
HincII GTy'rAC
Cuts at: 0 1016 1536
Size: 1016 520
HindIII A'AGCT_T
Cuts at: 0 914 1417 1536
Size: 914 503 119
Fragments arranged by size:
914 503 119
KasI G'GCGC_C
Cuts at: 0 605 1536
Size: 605 931
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1536
Size: 502 1034
MscI TGG'CCA
Cuts at: 0 533 662 1536
Size: 533 129 874
Fragments arranged by size:
874 533 129
MslI CAynn'nnrTG
Cuts at: 0 990 1536
Size: 990 546
MspA1I CmG'CkG
Cuts at: 0 108 595 764 866 1525 1536
Size: 108 487 169 102 659 11
Fragments arranged by size:
659 487 169 108 102 11
NaeI GCC'GGC
Cuts at: 0 9 1536
Size: 9 1527
NarI GG'CG_CC
Cuts at: 0 606 1536
Size: 606 930
NcoI C'CATG_G
Cuts at: 0 1115 1536
Size: 1115 421
NgoMIV G'CCGG_C
Cuts at: 0 7 1536
Size: 7 1529
NheI G'CTAG_C
Cuts at: 0 511 1536
Size: 511 1025
NsiI A_TGCA'T
Cuts at: 0 1004 1536
Size: 1004 532
PasI CC'CwG_GG
Cuts at: 0 967 1506 1536
Size: 967 539 30
Fragments arranged by size:
967 539 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 757 1051 1512 1536
Size: 757 294 461 24
Fragments arranged by size:
757 461 294 24
PfoI T'CCnGG_A
Cuts at: 0 122 631 1536
Size: 122 509 905
Fragments arranged by size:
905 509 122
PpuMI rG'GwC_Cy
Cuts at: 0 1501 1536
Size: 1501 35
PspOMI G'GGCC_C
Cuts at: 0 275 1536
Size: 275 1261
PstI C_TGCA'G
Cuts at: 0 137 992 1040 1536
Size: 137 855 48 496
Fragments arranged by size:
855 496 137 48
PvuII CAG'CTG
Cuts at: 0 108 595 866 1525 1536
Size: 108 487 271 659 11
Fragments arranged by size:
659 487 271 108 11
SacII CC_GC'GG
Cuts at: 0 765 1536
Size: 765 771
SfcI C'TryA_G
Cuts at: 0 12 133 825 988 1036 1536
Size: 12 121 692 163 48 500
Fragments arranged by size:
692 500 163 121 48 12
SfoI GGC'GCC
Cuts at: 0 607 1536
Size: 607 929
SmlI C'TyrA_G
Cuts at: 0 911 1211 1536
Size: 911 300 325
Fragments arranged by size:
911 325 300
StuI AGG'CCT
Cuts at: 0 842 1362 1536
Size: 842 520 174
Fragments arranged by size:
842 520 174
StyI C'CwwG_G
Cuts at: 0 1115 1536
Size: 1115 421
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 380 484 1302 1536
Size: 380 104 818 234
Fragments arranged by size:
818 380 234 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 459 491 1536
Size: 459 32 1045
Fragments arranged by size:
1045 459 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1113 1536
Size: 1113 423
Enzymes that do cut:
AcuI AleI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BfrBI BglI BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaHI BsaXI
BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I HincII
HindIII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NsiI PasI PflMI PfoI PpuMI
PspOMI PstI PvuII SacII SfcI SfoI SmlI StuI
StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AscI AseI AsiSI AvrII
BaeI BbvCI BcgI BciVI BclI BglII BmgBI BsaI
BsaAI BsaBI BsaWI BseYI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I
ClaI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HpaI KpnI MfeI
MluI NdeI NotI NruI NspI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI