Restriction map for
pF1KB7791
(Linear) (Six-base) MAPSORT of: KIBB7791.seq Check: 9761 from: 1 to: 1377
KIBB7791.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 583 1377
Size: 583 794
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 1128 1377
Size: 171 957 249
Fragments arranged by size:
957 249 171
AfeI AGC'GCT
Cuts at: 0 139 973 1377
Size: 139 834 404
Fragments arranged by size:
834 404 139
AgeI A'CCGG_T
Cuts at: 0 1270 1377
Size: 1270 107
AleI CACnn'nnGTG
Cuts at: 0 1035 1271 1377
Size: 1035 236 106
Fragments arranged by size:
1035 236 106
AlwNI CAG_nnn'CTG
Cuts at: 0 252 279 1214 1347 1377
Size: 252 27 935 133 30
Fragments arranged by size:
935 252 133 30 27
ApaI G_GGCC'C
Cuts at: 0 874 1377
Size: 874 503
ApaLI G'TGCA_C
Cuts at: 0 1096 1264 1377
Size: 1096 168 113
Fragments arranged by size:
1096 168 113
AvaI C'yCGr_G
Cuts at: 0 226 327 424 635 820 1377
Size: 226 101 97 211 185 557
Fragments arranged by size:
557 226 211 185 101 97
BamHI G'GATC_C
Cuts at: 0 442 1377
Size: 442 935
BanI G'GyrC_C
Cuts at: 0 649 768 777 914 1001 1377
Size: 649 119 9 137 87 376
Fragments arranged by size:
649 376 137 119 87 9
BanII G_rGCy'C
Cuts at: 0 206 731 820 874 988 1377
Size: 206 525 89 54 114 389
Fragments arranged by size:
525 389 206 114 89 54
BbeI G_GCGC'C
Cuts at: 0 918 1377
Size: 918 459
BbvCI CC'TCA_GC
Cuts at: 0 830 1377
Size: 830 547
BglI GCCn_nnn'nGGC
Cuts at: 0 791 1091 1162 1360 1377
Size: 791 300 71 198 17
Fragments arranged by size:
791 300 198 71 17
BlpI GC'TnA_GC
Cuts at: 0 665 1377
Size: 665 712
Bme1580I G_kGCm'C
Cuts at: 0 407 480 652 874 1042 1100 1268 1377
Size: 407 73 172 222 168 58 168 109
Fragments arranged by size:
407 222 172 168 168 109 73 58
BmgBI CAC'GTC
Cuts at: 0 801 1377
Size: 801 576
BmrI ACTGGGnnnn_n'
Cuts at: 0 868 1377
Size: 868 509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 289 834 1377
Size: 274 15 545 543
Fragments arranged by size:
545 543 274 15
Bpu10I CC'TnA_GC
Cuts at: 0 830 1377
Size: 830 547
BsaHI Gr'CG_yC
Cuts at: 0 915 1377
Size: 915 462
BsaWI w'CCGG_w
Cuts at: 0 1270 1377
Size: 1270 107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 444 474 1377
Size: 444 30 903
Fragments arranged by size:
903 444 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 355 427 838 1059 1377
Size: 355 72 411 221 318
Fragments arranged by size:
411 355 318 221 72
BseYI C'CCAG_C
Cuts at: 0 98 308 524 980 1364 1377
Size: 98 210 216 456 384 13
Fragments arranged by size:
456 384 216 210 98 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1333 1377
Size: 1333 44
BsiEI CG_ry'CG
Cuts at: 0 490 856 1377
Size: 490 366 521
Fragments arranged by size:
521 490 366
BsiHKAI G_wGCw'C
Cuts at: 0 206 1100 1268 1377
Size: 206 894 168 109
Fragments arranged by size:
894 206 168 109
BsmBI CGTCTCn'nnnn_
Cuts at: 0 556 1377
Size: 556 821
Bsp1286I G_dGCh'C
Cuts at: 0 206 407 480 652 731 820 874 988
Size: 206 201 73 172 79 89 54 114
Cuts at: 988 1042 1100 1268 1377
Size: 54 58 168 109
Fragments arranged by size:
206 201 172 168 114 109 89 79
73 58 54 54
BspHI T'CATG_A
Cuts at: 0 177 1377
Size: 177 1200
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 583 1253 1377
Size: 583 670 124
Fragments arranged by size:
670 583 124
BsrBI CCG'CTC
Cuts at: 0 1278 1377
Size: 1278 99
BsrFI r'CCGG_y
Cuts at: 0 771 851 911 1270 1377
Size: 771 80 60 359 107
Fragments arranged by size:
771 359 107 80 60
BssHII G'CGCG_C
Cuts at: 0 884 1320 1322 1377
Size: 884 436 2 55
Fragments arranged by size:
884 436 55 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1214 1377
Size: 1214 163
BstXI CCAn_nnnn'nTGG
Cuts at: 0 34 1377
Size: 34 1343
BstYI r'GATC_y
Cuts at: 0 248 299 442 672 1377
Size: 248 51 143 230 705
Fragments arranged by size:
705 248 230 143 51
BtgI C'CryG_G
Cuts at: 0 452 594 1370 1377
Size: 452 142 776 7
Fragments arranged by size:
776 452 142 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1317 1377
Size: 1317 60
BtsI GCAGTG_nn'
Cuts at: 0 967 1377
Size: 967 410
EaeI y'GGCC_r
Cuts at: 0 487 853 898 1163 1377
Size: 487 366 45 265 214
Fragments arranged by size:
487 366 265 214 45
EagI C'GGCC_G
Cuts at: 0 487 853 1377
Size: 487 366 524
Fragments arranged by size:
524 487 366
EarI CTCTTCn'nnn_
Cuts at: 0 542 682 1377
Size: 542 140 695
Fragments arranged by size:
695 542 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 433 1377
Size: 433 944
EcoICRI GAG'CTC
Cuts at: 0 204 1377
Size: 204 1173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 274 289 834 1128 1377
Size: 171 103 15 545 294 249
Fragments arranged by size:
545 294 249 171 103 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 757 1377
Size: 757 620
EcoO109I rG'GnC_Cy
Cuts at: 0 30 363 457 927 1377
Size: 30 333 94 470 450
Fragments arranged by size:
470 450 333 94 30
HaeII r_GCGC'y
Cuts at: 0 141 524 918 975 1157 1377
Size: 141 383 394 57 182 220
Fragments arranged by size:
394 383 220 182 141 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 391 423 1377
Size: 391 32 954
Fragments arranged by size:
954 391 32
KasI G'GCGC_C
Cuts at: 0 914 1377
Size: 914 463
MslI CAynn'nnrTG
Cuts at: 0 107 1035 1259 1271 1377
Size: 107 928 224 12 106
Fragments arranged by size:
928 224 107 106 12
MspA1I CmG'CkG
Cuts at: 0 215 669 953 980 1377
Size: 215 454 284 27 397
Fragments arranged by size:
454 397 284 215 27
NaeI GCC'GGC
Cuts at: 0 913 1377
Size: 913 464
NarI GG'CG_CC
Cuts at: 0 915 1377
Size: 915 462
NcoI C'CATG_G
Cuts at: 0 594 1377
Size: 594 783
NgoMIV G'CCGG_C
Cuts at: 0 911 1377
Size: 911 466
PasI CC'CwG_GG
Cuts at: 0 34 717 867 1377
Size: 34 683 150 510
Fragments arranged by size:
683 510 150 34
PflMI CCAn_nnn'nTGG
Cuts at: 0 1371 1377
Size: 1371 6
PfoI T'CCnGG_A
Cuts at: 0 251 555 703 811 923 1377
Size: 251 304 148 108 112 454
Fragments arranged by size:
454 304 251 148 112 108
PpuMI rG'GwC_Cy
Cuts at: 0 363 927 1377
Size: 363 564 450
Fragments arranged by size:
564 450 363
PspOMI G'GGCC_C
Cuts at: 0 870 1377
Size: 870 507
PstI C_TGCA'G
Cuts at: 0 435 761 1210 1377
Size: 435 326 449 167
Fragments arranged by size:
449 435 326 167
PvuII CAG'CTG
Cuts at: 0 953 1377
Size: 953 424
SacI G_AGCT'C
Cuts at: 0 206 1377
Size: 206 1171
SapI GCTCTTCn'nnn_
Cuts at: 0 542 1377
Size: 542 835
SexAI A'CCwGG_T
Cuts at: 0 1076 1377
Size: 1076 301
SfcI C'TryA_G
Cuts at: 0 367 431 757 1206 1377
Size: 367 64 326 449 171
Fragments arranged by size:
449 367 326 171 64
SfoI GGC'GCC
Cuts at: 0 916 1377
Size: 916 461
SgrAI Cr'CCGG_yG
Cuts at: 0 911 1270 1377
Size: 911 359 107
Fragments arranged by size:
911 359 107
SmaI CCC'GGG
Cuts at: 0 329 637 1377
Size: 329 308 740
Fragments arranged by size:
740 329 308
StuI AGG'CCT
Cuts at: 0 184 835 1377
Size: 184 651 542
Fragments arranged by size:
651 542 184
StyI C'CwwG_G
Cuts at: 0 11 239 353 594 1089 1377
Size: 11 228 114 241 495 288
Fragments arranged by size:
495 288 241 228 114 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 300 918 1377
Size: 300 618 459
Fragments arranged by size:
618 459 300
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 444 476 1377
Size: 444 32 901
Fragments arranged by size:
901 444 32
Tth111I GACn'n_nGTC
Cuts at: 0 807 1377
Size: 807 570
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 106 386 1377
Size: 106 280 991
Fragments arranged by size:
991 280 106
XmaI C'CCGG_G
Cuts at: 0 327 635 1377
Size: 327 308 742
Fragments arranged by size:
742 327 308
XmnI GAAnn'nnTTC
Cuts at: 0 50 1114 1198 1377
Size: 50 1064 84 179
Fragments arranged by size:
1064 179 84 50
Enzymes that do cut:
AarI AcuI AfeI AgeI AleI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbvCI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI BtgI
BtgZI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MslI MspA1I NaeI
NarI NcoI NgoMIV PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SapI SexAI SfcI SfoI SgrAI
SmaI StuI StyI TaqII TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AhdI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BbsI
BcgI BciVI BclI BfrBI BglII BmtI BplI BpuEI
BsaI BsaAI BsaBI BsiWI BsmI BspEI BsrDI BsrGI
BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SgfI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
XbaI XhoI ZraI