Restriction map for
pF1KB7792
(Linear) (Six-base) MAPSORT of: KIBB7792.seq Check: 3160 from: 1 to: 1557
KIBB7792.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AfeI AGC'GCT
Cuts at: 0 664 1557
Size: 664 893
AflIII A'CryG_T
Cuts at: 0 23 1478 1557
Size: 23 1455 79
Fragments arranged by size:
1455 79 23
AgeI A'CCGG_T
Cuts at: 0 1502 1557
Size: 1502 55
AleI CACnn'nnGTG
Cuts at: 0 605 1557
Size: 605 952
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 1557
Size: 416 32 1109
Fragments arranged by size:
1109 416 32
ApaI G_GGCC'C
Cuts at: 0 386 1557
Size: 386 1171
ApoI r'AATT_y
Cuts at: 0 841 1557
Size: 841 716
AvaI C'yCGr_G
Cuts at: 0 100 120 261 1002 1167 1557
Size: 100 20 141 741 165 390
Fragments arranged by size:
741 390 165 141 100 20
BanI G'GyrC_C
Cuts at: 0 1069 1143 1557
Size: 1069 74 414
Fragments arranged by size:
1069 414 74
BanII G_rGCy'C
Cuts at: 0 89 386 829 1557
Size: 89 297 443 728
Fragments arranged by size:
728 443 297 89
BbsI GAAGACnn'nnnn_
Cuts at: 0 758 1557
Size: 758 799
BglI GCCn_nnn'nGGC
Cuts at: 0 698 1557
Size: 698 859
BglII A'GATC_T
Cuts at: 0 778 1557
Size: 778 779
Bme1580I G_kGCm'C
Cuts at: 0 386 703 1072 1148 1557
Size: 386 317 369 76 409
Fragments arranged by size:
409 386 369 317 76
BmgBI CAC'GTC
Cuts at: 0 1422 1557
Size: 1422 135
BmrI ACTGGGnnnn_n'
Cuts at: 0 706 1557
Size: 706 851
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 126 1557
Size: 126 1431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 440 1557
Size: 53 387 1117
Fragments arranged by size:
1117 387 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1267 1557
Size: 1267 290
BsaI GGTCTCn'nnnn_
Cuts at: 0 1234 1557
Size: 1234 323
BsaAI yAC'GTr
Cuts at: 0 927 1557
Size: 927 630
BsaHI Gr'CG_yC
Cuts at: 0 557 997 1162 1557
Size: 557 440 165 395
Fragments arranged by size:
557 440 395 165
BsaWI w'CCGG_w
Cuts at: 0 1502 1557
Size: 1502 55
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1228 1303 1557
Size: 1228 75 254
Fragments arranged by size:
1228 254 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1033 1557
Size: 1012 21 524
Fragments arranged by size:
1012 524 21
BsiHKAI G_wGCw'C
Cuts at: 0 395 829 1295 1409 1541 1557
Size: 395 434 466 114 132 16
Fragments arranged by size:
466 434 395 132 114 16
BsmI GAATG_Cn'
Cuts at: 0 254 326 498 1557
Size: 254 72 172 1059
Fragments arranged by size:
1059 254 172 72
Bsp1286I G_dGCh'C
Cuts at: 0 89 386 395 703 829 1072 1148 1295
Size: 89 297 9 308 126 243 76 147
Cuts at: 1295 1409 1541 1557
Size: 114 132 16
Fragments arranged by size:
308 297 243 147 132 126 114 89
76 16 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 485 1124 1316 1557
Size: 485 639 192 241
Fragments arranged by size:
639 485 241 192
BsrBI CCG'CTC
Cuts at: 0 1059 1557
Size: 1059 498
BsrFI r'CCGG_y
Cuts at: 0 1502 1557
Size: 1502 55
BsrGI T'GTAC_A
Cuts at: 0 1382 1427 1557
Size: 1382 45 130
Fragments arranged by size:
1382 130 45
BssHII G'CGCG_C
Cuts at: 0 1264 1314 1557
Size: 1264 50 243
Fragments arranged by size:
1264 243 50
BssSI C'ACGA_G
Cuts at: 0 784 1557
Size: 784 773
BstAPI GCAn_nnn'nTGC
Cuts at: 0 446 1178 1481 1557
Size: 446 732 303 76
Fragments arranged by size:
732 446 303 76
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1440 1557
Size: 1440 117
BstYI r'GATC_y
Cuts at: 0 778 1557
Size: 778 779
Bsu36I CC'TnA_GG
Cuts at: 0 574 1557
Size: 574 983
BtgI C'CryG_G
Cuts at: 0 1106 1190 1449 1557
Size: 1106 84 259 108
Fragments arranged by size:
1106 259 108 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 95 1236 1557
Size: 95 1141 321
Fragments arranged by size:
1141 321 95
BtsI GCAGTG_nn'
Cuts at: 0 1395 1557
Size: 1395 162
EaeI y'GGCC_r
Cuts at: 0 158 567 618 1557
Size: 158 409 51 939
Fragments arranged by size:
939 409 158 51
EarI CTCTTCn'nnn_
Cuts at: 0 188 1414 1557
Size: 188 1226 143
Fragments arranged by size:
1226 188 143
EcoICRI GAG'CTC
Cuts at: 0 827 1557
Size: 827 730
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 440 1557
Size: 53 387 1117
Fragments arranged by size:
1117 387 53
EcoO109I rG'GnC_Cy
Cuts at: 0 105 452 858 1557
Size: 105 347 406 699
Fragments arranged by size:
699 406 347 105
EcoRV GAT'ATC
Cuts at: 0 360 1557
Size: 360 1197
HaeII r_GCGC'y
Cuts at: 0 666 890 1106 1557
Size: 666 224 216 451
Fragments arranged by size:
666 451 224 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1501 1533 1557
Size: 1501 32 24
Fragments arranged by size:
1501 32 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 890 1106 1557
Size: 300 590 216 451
Fragments arranged by size:
590 451 300 216
MscI TGG'CCA
Cuts at: 0 160 569 1557
Size: 160 409 988
Fragments arranged by size:
988 409 160
MslI CAynn'nnrTG
Cuts at: 0 605 1438 1557
Size: 605 833 119
Fragments arranged by size:
833 605 119
MspA1I CmG'CkG
Cuts at: 0 987 1047 1226 1364 1557
Size: 987 60 179 138 193
Fragments arranged by size:
987 193 179 138 60
NspI r_CATG'y
Cuts at: 0 27 1482 1557
Size: 27 1455 75
Fragments arranged by size:
1455 75 27
PciI A'CATG_T
Cuts at: 0 23 1478 1557
Size: 23 1455 79
Fragments arranged by size:
1455 79 23
PflMI CCAn_nnn'nTGG
Cuts at: 0 930 1557
Size: 930 627
PfoI T'CCnGG_A
Cuts at: 0 1412 1557
Size: 1412 145
PmlI CAC'GTG
Cuts at: 0 927 1557
Size: 927 630
PpuMI rG'GwC_Cy
Cuts at: 0 105 1557
Size: 105 1452
PspOMI G'GGCC_C
Cuts at: 0 382 1557
Size: 382 1175
PvuII CAG'CTG
Cuts at: 0 1047 1557
Size: 1047 510
SacI G_AGCT'C
Cuts at: 0 829 1557
Size: 829 728
SapI GCTCTTCn'nnn_
Cuts at: 0 1414 1557
Size: 1414 143
SmaI CCC'GGG
Cuts at: 0 1004 1557
Size: 1004 553
SmlI C'TyrA_G
Cuts at: 0 1282 1557
Size: 1282 275
StyI C'CwwG_G
Cuts at: 0 294 1130 1557
Size: 294 836 427
Fragments arranged by size:
836 427 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 478 1557
Size: 478 1079
TatI w'GTAC_w
Cuts at: 0 1382 1427 1557
Size: 1382 45 130
Fragments arranged by size:
1382 130 45
Tth111I GACn'n_nGTC
Cuts at: 0 151 1557
Size: 151 1406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 393 960 1137 1557
Size: 393 567 177 420
Fragments arranged by size:
567 420 393 177
XmaI C'CCGG_G
Cuts at: 0 1002 1557
Size: 1002 555
XmnI GAAnn'nnTTC
Cuts at: 0 514 1557
Size: 514 1043
Enzymes that do cut:
AfeI AflIII AgeI AleI AloI ApaI ApoI AvaI
BanI BanII BbsI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV HaeII
Hin4I MmeI MscI MslI MspA1I NspI PciI PflMI
PfoI PmlI PpuMI PspOMI PvuII SacI SapI SmaI
SmlI StyI TaqII TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AhdI
AlfI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI
BmtI Bpu10I BsaBI BsaXI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BsrDI BstBI BstEII BstZ17I ClaI CspCI
DraI DraIII DrdI EagI EciI EcoNI EcoRI FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI XbaI XhoI ZraI