Restriction map for
pF1KB7805
(Linear) (Six-base) MAPSORT of: KIBB7805.seq Check: 5447 from: 1 to: 1566
KIBB7805.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 23 1566
Size: 23 1543
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 1015 1077 1329 1566
Size: 323 692 62 252 237
Fragments arranged by size:
692 323 252 237 62
AflIII A'CryG_T
Cuts at: 0 1321 1566
Size: 1321 245
ApaI G_GGCC'C
Cuts at: 0 111 1566
Size: 111 1455
ApoI r'AATT_y
Cuts at: 0 593 623 707 791 1043 1127 1211 1295
Size: 593 30 84 84 252 84 84 84
Cuts at: 1295 1379 1566
Size: 84 187
Fragments arranged by size:
593 252 187 84 84 84 84 84
84 30
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 763 796 1566
Size: 763 33 770
Fragments arranged by size:
770 763 33
BanII G_rGCy'C
Cuts at: 0 97 111 481 769 1566
Size: 97 14 370 288 797
Fragments arranged by size:
797 370 288 97 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 549 1566
Size: 549 1017
BclI T'GATC_A
Cuts at: 0 495 1566
Size: 495 1071
BglI GCCn_nnn'nGGC
Cuts at: 0 106 1566
Size: 106 1460
BlpI GC'TnA_GC
Cuts at: 0 91 1566
Size: 91 1475
Bme1580I G_kGCm'C
Cuts at: 0 111 939 1566
Size: 111 828 627
Fragments arranged by size:
828 627 111
BmgBI CAC'GTC
Cuts at: 0 123 1566
Size: 123 1443
BmrI ACTGGGnnnn_n'
Cuts at: 0 166 1313 1566
Size: 166 1147 253
Fragments arranged by size:
1147 253 166
BmtI G_CTAG'C
Cuts at: 0 371 1566
Size: 371 1195
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 439 500 590 1566
Size: 439 61 90 976
Fragments arranged by size:
976 439 90 61
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 254 397 821 905 1073 1157 1241
Size: 151 103 143 424 84 168 84 84
Cuts at: 1241 1566
Size: 325
Fragments arranged by size:
424 325 168 151 143 103 84 84
84
BsaI GGTCTCn'nnnn_
Cuts at: 0 156 1566
Size: 156 1410
BsaAI yAC'GTr
Cuts at: 0 779 1566
Size: 779 787
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 759 1566
Size: 759 807
BseYI C'CCAG_C
Cuts at: 0 191 1566
Size: 191 1375
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 951 1566
Size: 951 615
BsiHKAI G_wGCw'C
Cuts at: 0 472 481 769 1566
Size: 472 9 288 797
Fragments arranged by size:
797 472 288 9
BsmI GAATG_Cn'
Cuts at: 0 823 1566
Size: 823 743
Bsp1286I G_dGCh'C
Cuts at: 0 97 111 472 481 769 939 1566
Size: 97 14 361 9 288 170 627
Fragments arranged by size:
627 361 288 170 97 14 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 23 1566
Size: 23 1543
BsrDI GCAATG_nn'
Cuts at: 0 391 996 1566
Size: 391 605 570
Fragments arranged by size:
605 570 391
BsrGI T'GTAC_A
Cuts at: 0 113 1566
Size: 113 1453
BstEII G'GTnAC_C
Cuts at: 0 39 1566
Size: 39 1527
BtgI C'CryG_G
Cuts at: 0 23 1566
Size: 23 1543
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 810 845 978 1013 1566
Size: 810 35 133 35 553
Fragments arranged by size:
810 553 133 35 35
DraI TTT'AAA
Cuts at: 0 900 1566
Size: 900 666
EarI CTCTTCn'nnn_
Cuts at: 0 71 491 1110 1566
Size: 71 420 619 456
Fragments arranged by size:
619 456 420 71
EcoICRI GAG'CTC
Cuts at: 0 479 767 1566
Size: 479 288 799
Fragments arranged by size:
799 479 288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 254 323 397 821 905 1015 1073
Size: 151 103 69 74 424 84 110 58
Cuts at: 1073 1077 1157 1241 1329 1566
Size: 4 80 84 88 237
Fragments arranged by size:
424 237 151 110 103 88 84 84
80 74 69 58 4
EcoO109I rG'GnC_Cy
Cuts at: 0 107 108 169 1566
Size: 107 1 61 1397
Fragments arranged by size:
1397 107 61 1
EcoRI G'AATT_C
Cuts at: 0 707 1043 1127 1295 1379 1566
Size: 707 336 84 168 84 187
Fragments arranged by size:
707 336 187 168 84 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1174 1206 1440 1472 1566
Size: 1174 32 234 32 94
Fragments arranged by size:
1174 234 94 32 32
HindIII A'AGCT_T
Cuts at: 0 836 1172 1508 1566
Size: 836 336 336 58
Fragments arranged by size:
836 336 336 58
MspA1I CmG'CkG
Cuts at: 0 90 1566
Size: 90 1476
NcoI C'CATG_G
Cuts at: 0 23 1566
Size: 23 1543
NdeI CA'TA_TG
Cuts at: 0 1330 1566
Size: 1330 236
NheI G'CTAG_C
Cuts at: 0 367 1566
Size: 367 1199
NspI r_CATG'y
Cuts at: 0 1325 1566
Size: 1325 241
PciI A'CATG_T
Cuts at: 0 1321 1566
Size: 1321 245
PfoI T'CCnGG_A
Cuts at: 0 29 463 1566
Size: 29 434 1103
Fragments arranged by size:
1103 434 29
PpuMI rG'GwC_Cy
Cuts at: 0 169 1566
Size: 169 1397
PsiI TTA'TAA
Cuts at: 0 1151 1566
Size: 1151 415
PspOMI G'GGCC_C
Cuts at: 0 107 1566
Size: 107 1459
PvuII CAG'CTG
Cuts at: 0 90 1566
Size: 90 1476
SacI G_AGCT'C
Cuts at: 0 481 769 1566
Size: 481 288 797
Fragments arranged by size:
797 481 288
SexAI A'CCwGG_T
Cuts at: 0 146 1566
Size: 146 1420
SfcI C'TryA_G
Cuts at: 0 138 1566
Size: 138 1428
SnaBI TAC'GTA
Cuts at: 0 779 1566
Size: 779 787
StuI AGG'CCT
Cuts at: 0 754 1090 1566
Size: 754 336 476
Fragments arranged by size:
754 476 336
StyI C'CwwG_G
Cuts at: 0 23 1566
Size: 23 1543
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 85 1566
Size: 85 1481
TatI w'GTAC_w
Cuts at: 0 113 1018 1566
Size: 113 905 548
Fragments arranged by size:
905 548 113
XmnI GAAnn'nnTTC
Cuts at: 0 1090 1174 1566
Size: 1090 84 392
Fragments arranged by size:
1090 392 84
Enzymes that do cut:
AarI AcuI AflIII ApaI ApoI BaeI BanII BbsI
BclI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BsaI BsaAI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrGI BstEII BtgI CspCI DraI
EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HindIII MspA1I
NcoI NdeI NheI NspI PciI PfoI PpuMI PsiI
PspOMI PvuII SacI SexAI SfcI SnaBI StuI StyI
TaqII TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanI BbeI BbvCI BcgI BciVI
BfrBI BglII Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI DraIII DrdI EaeI EagI EciI EcoNI
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI NaeI
NarI NgoMIV NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PshAI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI