Restriction map for
pF1KB7813
(Linear) (Six-base) MAPSORT of: KIBB7813.seq Check: 7822 from: 1 to: 1596
KIBB7813.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AatII G_ACGT'C
Cuts at: 0 365 1596
Size: 365 1231
AccI GT'mk_AC
Cuts at: 0 1191 1596
Size: 1191 405
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1073 1399 1596
Size: 1073 326 197
Fragments arranged by size:
1073 326 197
AflIII A'CryG_T
Cuts at: 0 49 1596
Size: 49 1547
AhdI GACnn_n'nnGTC
Cuts at: 0 808 1269 1596
Size: 808 461 327
Fragments arranged by size:
808 461 327
AleI CACnn'nnGTG
Cuts at: 0 422 1596
Size: 422 1174
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 419 451 1596
Size: 419 32 1145
Fragments arranged by size:
1145 419 32
ApaI G_GGCC'C
Cuts at: 0 389 1596
Size: 389 1207
ApoI r'AATT_y
Cuts at: 0 159 1596
Size: 159 1437
BanI G'GyrC_C
Cuts at: 0 789 1596
Size: 789 807
BanII G_rGCy'C
Cuts at: 0 389 1375 1596
Size: 389 986 221
Fragments arranged by size:
986 389 221
BbsI GAAGACnn'nnnn_
Cuts at: 0 1389 1596
Size: 1389 207
BbvCI CC'TCA_GC
Cuts at: 0 1007 1596
Size: 1007 589
BciVI GTATCCnnnnn_n'
Cuts at: 0 1344 1596
Size: 1344 252
BfrBI ATG'CAT
Cuts at: 0 3 1596
Size: 3 1593
BlpI GC'TnA_GC
Cuts at: 0 944 1074 1596
Size: 944 130 522
Fragments arranged by size:
944 522 130
Bme1580I G_kGCm'C
Cuts at: 0 389 1596
Size: 389 1207
BmrI ACTGGGnnnn_n'
Cuts at: 0 1057 1596
Size: 1057 539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 667 670 1596
Size: 527 140 3 926
Fragments arranged by size:
926 527 140 3
Bpu10I CC'TnA_GC
Cuts at: 0 211 1007 1596
Size: 211 796 589
Fragments arranged by size:
796 589 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 1596
Size: 93 1503
BsaI GGTCTCn'nnnn_
Cuts at: 0 795 1596
Size: 795 801
BsaHI Gr'CG_yC
Cuts at: 0 362 560 1596
Size: 362 198 1036
Fragments arranged by size:
1036 362 198
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1347 1377 1596
Size: 1347 30 219
Fragments arranged by size:
1347 219 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 667 1596
Size: 667 929
BseYI C'CCAG_C
Cuts at: 0 706 1216 1317 1596
Size: 706 510 101 279
Fragments arranged by size:
706 510 279 101
BsiHKAI G_wGCw'C
Cuts at: 0 659 1442 1586 1596
Size: 659 783 144 10
Fragments arranged by size:
783 659 144 10
BsmI GAATG_Cn'
Cuts at: 0 1522 1596
Size: 1522 74
Bsp1286I G_dGCh'C
Cuts at: 0 389 659 1375 1442 1586 1596
Size: 389 270 716 67 144 10
Fragments arranged by size:
716 389 270 144 67 10
BspHI T'CATG_A
Cuts at: 0 716 1143 1596
Size: 716 427 453
Fragments arranged by size:
716 453 427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 702 813 1596
Size: 702 111 783
Fragments arranged by size:
783 702 111
BsrDI GCAATG_nn'
Cuts at: 0 3 1596
Size: 3 1593
BsrGI T'GTAC_A
Cuts at: 0 1472 1596
Size: 1472 124
BstAPI GCAn_nnn'nTGC
Cuts at: 0 377 1226 1596
Size: 377 849 370
Fragments arranged by size:
849 377 370
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1485 1596
Size: 1485 111
Bsu36I CC'TnA_GG
Cuts at: 0 577 1367 1596
Size: 577 790 229
Fragments arranged by size:
790 577 229
BtgI C'CryG_G
Cuts at: 0 1494 1596
Size: 1494 102
BtsI GCAGTG_nn'
Cuts at: 0 1440 1596
Size: 1440 156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 600 635 1509 1544 1596
Size: 600 35 874 35 52
Fragments arranged by size:
874 600 52 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1187 1596
Size: 1187 409
EarI CTCTTCn'nnn_
Cuts at: 0 185 1596
Size: 185 1411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 667 670 1073 1399 1596
Size: 527 140 3 403 326 197
Fragments arranged by size:
527 403 326 197 140 3
EcoO109I rG'GnC_Cy
Cuts at: 0 581 1011 1424 1506 1596
Size: 581 430 413 82 90
Fragments arranged by size:
581 430 413 90 82
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1094 1337 1596
Size: 1094 243 259
Fragments arranged by size:
1094 259 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 794 826 942 974 1255 1287
Size: 91 32 671 32 116 32 281 32
Cuts at: 1287 1546 1578 1596
Size: 259 32 18
Fragments arranged by size:
671 281 259 116 91 32 32 32
32 32 18
HincII GTy'rAC
Cuts at: 0 1192 1596
Size: 1192 404
HindIII A'AGCT_T
Cuts at: 0 1343 1596
Size: 1343 253
MfeI C'AATT_G
Cuts at: 0 95 1596
Size: 95 1501
MslI CAynn'nnrTG
Cuts at: 0 422 1483 1515 1596
Size: 422 1061 32 81
Fragments arranged by size:
1061 422 81 32
NcoI C'CATG_G
Cuts at: 0 1494 1596
Size: 1494 102
NdeI CA'TA_TG
Cuts at: 0 930 962 1596
Size: 930 32 634
Fragments arranged by size:
930 634 32
NsiI A_TGCA'T
Cuts at: 0 5 1596
Size: 5 1591
NspI r_CATG'y
Cuts at: 0 133 1596
Size: 133 1463
PflMI CCAn_nnn'nTGG
Cuts at: 0 591 1596
Size: 591 1005
PfoI T'CCnGG_A
Cuts at: 0 305 1596
Size: 305 1291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 502 534 1596
Size: 502 32 1062
Fragments arranged by size:
1062 502 32
PpuMI rG'GwC_Cy
Cuts at: 0 581 1506 1596
Size: 581 925 90
Fragments arranged by size:
925 581 90
PsiI TTA'TAA
Cuts at: 0 1069 1596
Size: 1069 527
PspOMI G'GGCC_C
Cuts at: 0 385 1596
Size: 385 1211
SalI G'TCGA_C
Cuts at: 0 1190 1596
Size: 1190 406
SanDI GG'GwC_CC
Cuts at: 0 1506 1596
Size: 1506 90
SfcI C'TryA_G
Cuts at: 0 534 546 1533 1596
Size: 534 12 987 63
Fragments arranged by size:
987 534 63 12
SmlI C'TyrA_G
Cuts at: 0 108 1596
Size: 108 1488
SphI G_CATG'C
Cuts at: 0 133 1596
Size: 133 1463
SspI AAT'ATT
Cuts at: 0 780 1596
Size: 780 816
StuI AGG'CCT
Cuts at: 0 1210 1596
Size: 1210 386
StyI C'CwwG_G
Cuts at: 0 999 1278 1358 1494 1596
Size: 999 279 80 136 102
Fragments arranged by size:
999 279 136 102 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 132 1596
Size: 132 1464
TatI w'GTAC_w
Cuts at: 0 1472 1596
Size: 1472 124
Tth111I GACn'n_nGTC
Cuts at: 0 1465 1596
Size: 1465 131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 1596
Size: 396 1200
XmnI GAAnn'nnTTC
Cuts at: 0 517 1596
Size: 517 1079
ZraI GAC'GTC
Cuts at: 0 363 1596
Size: 363 1233
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AleI AloI ApaI
ApoI BanI BanII BbsI BbvCI BciVI BfrBI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrGI BstAPI BstXI Bsu36I BtgI BtsI CspCI DrdI
EarI Eco57MI EcoO109I FalI Hin4I HincII HindIII MfeI
MslI NcoI NdeI NsiI NspI PflMI PfoI PpiI
PpuMI PsiI PspOMI SalI SanDI SfcI SmlI SphI
SspI StuI StyI TaqII TatI Tth111I XcmI XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AlfI AlwNI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BcgI BclI BglI BglII BmgBI BmtI BplI
BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I
BtgZI ClaI DraI DraIII EaeI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HpaI
KasI KpnI MluI MmeI MscI MspA1I NaeI NarI
NgoMIV NheI NotI NruI PacI PasI PciI PmeI
PmlI PshAI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TstI
XbaI XhoI XmaI