(Linear) (Six-base) MAPSORT of: KIBB7815.seq  Check: 882  from: 1  to: 1596



KIBB7815.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AfeI AGC'GCT

Cuts at:      0     268    1596 
   Size:        268    1328
 
AflIII A'CryG_T

Cuts at:      0     991    1596 
   Size:        991     605
 
AleI CACnn'nnGTG

Cuts at:      0    1340    1596 
   Size:       1340     256
 
AlwNI CAG_nnn'CTG

Cuts at:      0     360    1596 
   Size:        360    1236
 
ApoI r'AATT_y

Cuts at:      0     529     929    1097    1596 
   Size:        529     400     168     499

  Fragments arranged by size: 

                 529     499     400     168
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     994    1596 
   Size:        994     602
 
BclI T'GATC_A

Cuts at:      0     252     666    1559    1596 
   Size:        252     414     893      37

  Fragments arranged by size: 

                 893     414     252      37
 
BfrBI ATG'CAT

Cuts at:      0     322     681    1596 
   Size:        322     359     915

  Fragments arranged by size: 

                 915     359     322
 
BmgBI CAC'GTC

Cuts at:      0     992    1596 
   Size:        992     604
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1380    1596 
   Size:       1380     216
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     283     714    1596 
   Size:         49     234     431     882

  Fragments arranged by size: 

                 882     431     234      49
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     799    1596 
   Size:        799     797
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1084    1191    1596 
   Size:       1084     107     405

  Fragments arranged by size: 

                1084     405     107
 
BsaAI yAC'GTr

Cuts at:      0     394    1596 
   Size:        394    1202
 
BsaHI Gr'CG_yC

Cuts at:      0    1030    1596 
   Size:       1030     566
 
BsaWI w'CCGG_w

Cuts at:      0     159     853    1596 
   Size:        159     694     743

  Fragments arranged by size: 

                 743     694     159
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73    1423    1596 
   Size:         73    1350     173

  Fragments arranged by size: 

                1350     173      73
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     642    1062    1502    1596 
   Size:        642     420     440      94

  Fragments arranged by size: 

                 642     440     420      94
 
BsmI GAATG_Cn'

Cuts at:      0     793    1536    1596 
   Size:        793     743      60

  Fragments arranged by size: 

                 793     743      60
 
BspHI T'CATG_A

Cuts at:      0     309     375    1596 
   Size:        309      66    1221

  Fragments arranged by size: 

                1221     309      66
 
BsrDI GCAATG_nn'

Cuts at:      0     339     966    1596 
   Size:        339     627     630

  Fragments arranged by size: 

                 630     627     339
 
BsrGI T'GTAC_A

Cuts at:      0     918    1596 
   Size:        918     678
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      27    1596 
   Size:         27    1569
 
Bsu36I CC'TnA_GG

Cuts at:      0    1488    1596 
   Size:       1488     108
 
BtsI GCAGTG_nn'

Cuts at:      0     143     630     882    1050    1541    1596 
   Size:        143     487     252     168     491      55

  Fragments arranged by size: 

                 491     487     252     168     143      55
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     948     983    1596 
   Size:        948      35     613

  Fragments arranged by size: 

                 948     613      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     175    1596 
   Size:        175    1421
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     283     714    1596 
   Size:         49     234     431     882

  Fragments arranged by size: 

                 882     431     234      49
 
EcoO109I rG'GnC_Cy

Cuts at:      0      90    1596 
   Size:         90    1506
 
EcoRI G'AATT_C

Cuts at:      0     929    1097    1596 
   Size:        929     168     499

  Fragments arranged by size: 

                 929     499     168
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1050    1596 
   Size:       1050     546
 
HaeII r_GCGC'y

Cuts at:      0      80     270    1596 
   Size:         80     190    1326

  Fragments arranged by size: 

                1326     190      80
 
HincII GTy'rAC

Cuts at:      0     429    1596 
   Size:        429    1167
 
HpaI GTT'AAC

Cuts at:      0     429    1596 
   Size:        429    1167
 
MfeI C'AATT_G

Cuts at:      0     203    1596 
   Size:        203    1393
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     158    1596 
   Size:         92      66    1438

  Fragments arranged by size: 

                1438      92      66
 
MslI CAynn'nnrTG

Cuts at:      0     314     938    1340    1596 
   Size:        314     624     402     256

  Fragments arranged by size: 

                 624     402     314     256
 
MspA1I CmG'CkG

Cuts at:      0    1427    1596 
   Size:       1427     169
 
NdeI CA'TA_TG

Cuts at:      0     241    1596 
   Size:        241    1355
 
NsiI A_TGCA'T

Cuts at:      0     324     683    1596 
   Size:        324     359     913

  Fragments arranged by size: 

                 913     359     324
 
PpuMI rG'GwC_Cy

Cuts at:      0      90    1596 
   Size:         90    1506
 
PstI C_TGCA'G

Cuts at:      0    1317    1596 
   Size:       1317     279
 
SexAI A'CCwGG_T

Cuts at:      0     128     671    1596 
   Size:        128     543     925

  Fragments arranged by size: 

                 925     543     128
 
SfcI C'TryA_G

Cuts at:      0     120    1313    1596 
   Size:        120    1193     283

  Fragments arranged by size: 

                1193     283     120
 
SmlI C'TyrA_G

Cuts at:      0     814    1596 
   Size:        814     782
 
SspI AAT'ATT

Cuts at:      0     333    1596 
   Size:        333    1263
 
StyI C'CwwG_G

Cuts at:      0    1295    1596 
   Size:       1295     301
 
TatI w'GTAC_w

Cuts at:      0     918    1596 
   Size:        918     678
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1454    1486    1596 
   Size:       1454      32     110

  Fragments arranged by size: 

                1454     110      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     512     640     724    1596 
   Size:        512     128      84     872

  Fragments arranged by size: 

                 872     512     128      84
 

 Enzymes that do cut:

     AfeI   AflIII     AleI    AlwNI     ApoI     BbsI     BclI    BfrBI
    BmgBI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BseRI     BsgI     BsmI    BspHI    BsrDI    BsrGI    BstXI   Bsu36I
     BtsI    CspCI     EarI  Eco57MI EcoO109I    EcoRI     FalI    HaeII
   HincII     HpaI     MfeI     MmeI     MslI   MspA1I     NdeI     NsiI
    PpuMI     PstI    SexAI     SfcI     SmlI     SspI     StyI     TatI
     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI
   Bpu10I    BsaBI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I     BtgI    BtgZI     ClaI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRV
     FseI     FspI    FspAI    Hin4I  HindIII     KasI     KpnI     MluI
     MscI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI