Restriction map for
pF1KB7818
(Linear) (Six-base) MAPSORT of: KIBB7818.seq Check: 1585 from: 1 to: 1794
KIBB7818.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 323 729 1794
Size: 323 406 1065
Fragments arranged by size:
1065 406 323
AatII G_ACGT'C
Cuts at: 0 218 1794
Size: 218 1576
AccI GT'mk_AC
Cuts at: 0 1118 1794
Size: 1118 676
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 1794
Size: 162 1632
AflIII A'CryG_T
Cuts at: 0 716 890 1794
Size: 716 174 904
Fragments arranged by size:
904 716 174
AleI CACnn'nnGTG
Cuts at: 0 556 739 1794
Size: 556 183 1055
Fragments arranged by size:
1055 556 183
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 896 1794
Size: 896 898
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1325 1357 1794
Size: 1325 32 437
Fragments arranged by size:
1325 437 32
ApaI G_GGCC'C
Cuts at: 0 590 1113 1794
Size: 590 523 681
Fragments arranged by size:
681 590 523
ApoI r'AATT_y
Cuts at: 0 1462 1794
Size: 1462 332
AvaI C'yCGr_G
Cuts at: 0 396 435 696 1794
Size: 396 39 261 1098
Fragments arranged by size:
1098 396 261 39
BanI G'GyrC_C
Cuts at: 0 550 741 1743 1794
Size: 550 191 1002 51
Fragments arranged by size:
1002 550 191 51
BanII G_rGCy'C
Cuts at: 0 590 617 766 1054 1064 1113 1553 1794
Size: 590 27 149 288 10 49 440 241
Fragments arranged by size:
590 440 288 241 149 49 27 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 284 1742 1794
Size: 284 1458 52
Fragments arranged by size:
1458 284 52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1408 1442 1483 1517 1794
Size: 1408 34 41 34 277
Fragments arranged by size:
1408 277 41 34 34
BclI T'GATC_A
Cuts at: 0 1091 1794
Size: 1091 703
Bme1580I G_kGCm'C
Cuts at: 0 590 662 746 1101 1113 1794
Size: 590 72 84 355 12 681
Fragments arranged by size:
681 590 355 84 72 12
BmgBI CAC'GTC
Cuts at: 0 597 1794
Size: 597 1197
BmrI ACTGGGnnnn_n'
Cuts at: 0 318 910 1363 1794
Size: 318 592 453 431
Fragments arranged by size:
592 453 431 318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 1794
Size: 92 1702
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 1537 1794
Size: 20 1517 257
Fragments arranged by size:
1517 257 20
BsaAI yAC'GTr
Cuts at: 0 468 633 891 1614 1794
Size: 468 165 258 723 180
Fragments arranged by size:
723 468 258 180 165
BsaHI Gr'CG_yC
Cuts at: 0 215 385 1794
Size: 215 170 1409
Fragments arranged by size:
1409 215 170
BsaWI w'CCGG_w
Cuts at: 0 250 1794
Size: 250 1544
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 355 385 1014 1044 1794
Size: 355 30 629 30 750
Fragments arranged by size:
750 629 355 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 363 1458 1794
Size: 346 17 1095 336
Fragments arranged by size:
1095 346 336 17
BseYI C'CCAG_C
Cuts at: 0 172 1199 1256 1794
Size: 172 1027 57 538
Fragments arranged by size:
1027 538 172 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 577 1794
Size: 430 147 1217
Fragments arranged by size:
1217 430 147
BsmBI CGTCTCn'nnnn_
Cuts at: 0 725 1794
Size: 725 1069
Bsp1286I G_dGCh'C
Cuts at: 0 590 617 662 746 766 1054 1064 1101
Size: 590 27 45 84 20 288 10 37
Cuts at: 1101 1113 1553 1794
Size: 12 440 241
Fragments arranged by size:
590 440 288 241 84 45 37 27
20 12 10
BspEI T'CCGG_A
Cuts at: 0 250 1794
Size: 250 1544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 323 729 1295 1316 1794
Size: 323 406 566 21 478
Fragments arranged by size:
566 478 406 323 21
BsrDI GCAATG_nn'
Cuts at: 0 1401 1426 1514 1794
Size: 1401 25 88 280
Fragments arranged by size:
1401 280 88 25
BsrFI r'CCGG_y
Cuts at: 0 619 1794
Size: 619 1175
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1416 1794
Size: 1416 378
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1371 1569 1665 1794
Size: 1371 198 96 129
Fragments arranged by size:
1371 198 129 96
BstYI r'GATC_y
Cuts at: 0 439 1268 1794
Size: 439 829 526
Fragments arranged by size:
829 526 439
BtgI C'CryG_G
Cuts at: 0 686 1238 1794
Size: 686 552 556
Fragments arranged by size:
686 556 552
BtsI GCAGTG_nn'
Cuts at: 0 152 1794
Size: 152 1642
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 212 247 1794
Size: 212 35 1547
Fragments arranged by size:
1547 212 35
DraI TTT'AAA
Cuts at: 0 1013 1794
Size: 1013 781
DraIII CAC_nnn'GTG
Cuts at: 0 633 636 1794
Size: 633 3 1158
Fragments arranged by size:
1158 633 3
EaeI y'GGCC_r
Cuts at: 0 470 711 1794
Size: 470 241 1083
Fragments arranged by size:
1083 470 241
EarI CTCTTCn'nnn_
Cuts at: 0 81 529 1042 1794
Size: 81 448 513 752
Fragments arranged by size:
752 513 448 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 162 1794
Size: 92 70 1632
Fragments arranged by size:
1632 92 70
EcoO109I rG'GnC_Cy
Cuts at: 0 26 587 1109 1794
Size: 26 561 522 685
Fragments arranged by size:
685 561 522 26
EcoRI G'AATT_C
Cuts at: 0 1462 1794
Size: 1462 332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1327 1794
Size: 1327 467
FspI TGC'GCA
Cuts at: 0 832 1794
Size: 832 962
FspAI rTGC'GCAy
Cuts at: 0 832 1794
Size: 832 962
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 424 456 1535 1567 1794
Size: 424 32 1079 32 227
Fragments arranged by size:
1079 424 227 32 32
HincII GTy'rAC
Cuts at: 0 1119 1794
Size: 1119 675
MluI A'CGCG_T
Cuts at: 0 716 1794
Size: 716 1078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 1148 1388 1496 1794
Size: 797 351 240 108 298
Fragments arranged by size:
797 351 298 240 108
MscI TGG'CCA
Cuts at: 0 472 1794
Size: 472 1322
MslI CAynn'nnrTG
Cuts at: 0 286 556 739 1794
Size: 286 270 183 1055
Fragments arranged by size:
1055 286 270 183
MspA1I CmG'CkG
Cuts at: 0 726 1794
Size: 726 1068
NaeI GCC'GGC
Cuts at: 0 621 1794
Size: 621 1173
NcoI C'CATG_G
Cuts at: 0 686 1238 1794
Size: 686 552 556
Fragments arranged by size:
686 556 552
NgoMIV G'CCGG_C
Cuts at: 0 619 1794
Size: 619 1175
PflMI CCAn_nnn'nTGG
Cuts at: 0 633 636 1794
Size: 633 3 1158
Fragments arranged by size:
1158 633 3
PfoI T'CCnGG_A
Cuts at: 0 1775 1794
Size: 1775 19
PmlI CAC'GTG
Cuts at: 0 633 1614 1794
Size: 633 981 180
Fragments arranged by size:
981 633 180
PpuMI rG'GwC_Cy
Cuts at: 0 26 1794
Size: 26 1768
PshAI GACnn'nnGTC
Cuts at: 0 258 926 1794
Size: 258 668 868
Fragments arranged by size:
868 668 258
PspOMI G'GGCC_C
Cuts at: 0 586 1109 1794
Size: 586 523 685
Fragments arranged by size:
685 586 523
PstI C_TGCA'G
Cuts at: 0 707 1794
Size: 707 1087
PvuII CAG'CTG
Cuts at: 0 726 1794
Size: 726 1068
SalI G'TCGA_C
Cuts at: 0 1117 1794
Size: 1117 677
SapI GCTCTTCn'nnn_
Cuts at: 0 1042 1794
Size: 1042 752
ScaI AGT'ACT
Cuts at: 0 949 1794
Size: 949 845
SfcI C'TryA_G
Cuts at: 0 63 198 703 1708 1794
Size: 63 135 505 1005 86
Fragments arranged by size:
1005 505 135 86 63
SmaI CCC'GGG
Cuts at: 0 398 437 698 1794
Size: 398 39 261 1096
Fragments arranged by size:
1096 398 261 39
SmlI C'TyrA_G
Cuts at: 0 35 1552 1794
Size: 35 1517 242
Fragments arranged by size:
1517 242 35
StyI C'CwwG_G
Cuts at: 0 686 1238 1794
Size: 686 552 556
Fragments arranged by size:
686 556 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1213 1794
Size: 1213 581
TatI w'GTAC_w
Cuts at: 0 947 1794
Size: 947 847
XmaI C'CCGG_G
Cuts at: 0 396 435 696 1794
Size: 396 39 261 1098
Fragments arranged by size:
1098 396 261 39
XmnI GAAnn'nnTTC
Cuts at: 0 281 995 1794
Size: 281 714 799
Fragments arranged by size:
799 714 281
ZraI GAC'GTC
Cuts at: 0 216 1794
Size: 216 1578
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AloI
ApaI ApoI AvaI BanI BanII BbsI BcgI BclI
Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspEI BspMI
BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI CspCI
DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRI FalI
FspI FspAI Hin4I HincII MluI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV PflMI PfoI PmlI PpuMI
PshAI PspOMI PstI PvuII SalI SapI ScaI SfcI
SmaI SmlI StyI TaqII TatI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AlwNI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BciVI BfrBI BglI BglII BlpI BmtI BplI Bpu10I
BsaI BsaBI BsiEI BsiHKAI BsiWI BsmI BspHI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI DrdI EagI EciI EcoICRI EcoNI EcoRV FseI
HaeII HindIII HpaI KasI KpnI MfeI NarI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacI
SacII SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI