Restriction map for
pF1KB7820
(Linear) (Six-base) MAPSORT of: KIBB7820.seq Check: 5424 from: 1 to: 1797
KIBB7820.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 926 1797
Size: 926 871
AccI GT'mk_AC
Cuts at: 0 212 1797
Size: 212 1585
Acc65I G'GTAC_C
Cuts at: 0 1087 1245 1797
Size: 1087 158 552
Fragments arranged by size:
1087 552 158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 661 694 1576 1797
Size: 358 303 33 882 221
Fragments arranged by size:
882 358 303 221 33
AflII C'TTAA_G
Cuts at: 0 1639 1797
Size: 1639 158
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 593 1797
Size: 593 1204
AlwNI CAG_nnn'CTG
Cuts at: 0 261 748 1392 1797
Size: 261 487 644 405
Fragments arranged by size:
644 487 405 261
ApoI r'AATT_y
Cuts at: 0 1256 1600 1691 1797
Size: 1256 344 91 106
Fragments arranged by size:
1256 344 106 91
AvaI C'yCGr_G
Cuts at: 0 54 415 1797
Size: 54 361 1382
Fragments arranged by size:
1382 361 54
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1079 1112 1797
Size: 1079 33 685
Fragments arranged by size:
1079 685 33
BamHI G'GATC_C
Cuts at: 0 386 1109 1797
Size: 386 723 688
Fragments arranged by size:
723 688 386
BanI G'GyrC_C
Cuts at: 0 43 1087 1245 1797
Size: 43 1044 158 552
Fragments arranged by size:
1044 552 158 43
BanII G_rGCy'C
Cuts at: 0 104 1472 1797
Size: 104 1368 325
Fragments arranged by size:
1368 325 104
BbeI G_GCGC'C
Cuts at: 0 47 1797
Size: 47 1750
BbvCI CC'TCA_GC
Cuts at: 0 662 1797
Size: 662 1135
BclI T'GATC_A
Cuts at: 0 125 1797
Size: 125 1672
BglI GCCn_nnn'nGGC
Cuts at: 0 872 1045 1797
Size: 872 173 752
Fragments arranged by size:
872 752 173
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1142 1797
Size: 1142 655
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 1062 1486 1797
Size: 2 1060 424 311
Fragments arranged by size:
1060 424 311 2
Bpu10I CC'TnA_GC
Cuts at: 0 548 662 1797
Size: 548 114 1135
Fragments arranged by size:
1135 548 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1797
Size: 280 1517
BsaHI Gr'CG_yC
Cuts at: 0 44 50 1797
Size: 44 6 1747
Fragments arranged by size:
1747 44 6
BsaWI w'CCGG_w
Cuts at: 0 383 1797
Size: 383 1414
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1357 1387 1797
Size: 1357 30 410
Fragments arranged by size:
1357 410 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1715 1797
Size: 1715 82
BseYI C'CCAG_C
Cuts at: 0 399 1797
Size: 399 1398
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 1797
Size: 806 991
BsiHKAI G_wGCw'C
Cuts at: 0 888 1472 1797
Size: 888 584 325
Fragments arranged by size:
888 584 325
Bsp1286I G_dGCh'C
Cuts at: 0 104 888 1472 1797
Size: 104 784 584 325
Fragments arranged by size:
784 584 325 104
BspEI T'CCGG_A
Cuts at: 0 383 1797
Size: 383 1414
BspHI T'CATG_A
Cuts at: 0 1728 1797
Size: 1728 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 369 926 1797
Size: 369 557 871
Fragments arranged by size:
871 557 369
BsrBI CCG'CTC
Cuts at: 0 1107 1797
Size: 1107 690
BsrDI GCAATG_nn'
Cuts at: 0 1013 1309 1797
Size: 1013 296 488
Fragments arranged by size:
1013 488 296
BsrFI r'CCGG_y
Cuts at: 0 46 1797
Size: 46 1751
BsrGI T'GTAC_A
Cuts at: 0 326 1797
Size: 326 1471
BstAPI GCAn_nnn'nTGC
Cuts at: 0 997 1311 1797
Size: 997 314 486
Fragments arranged by size:
997 486 314
BstBI TT'CG_AA
Cuts at: 0 455 1797
Size: 455 1342
BstXI CCAn_nnnn'nTGG
Cuts at: 0 933 1797
Size: 933 864
BstYI r'GATC_y
Cuts at: 0 386 936 1109 1797
Size: 386 550 173 688
Fragments arranged by size:
688 550 386 173
Bsu36I CC'TnA_GG
Cuts at: 0 1669 1797
Size: 1669 128
BtgI C'CryG_G
Cuts at: 0 359 393 1001 1361 1797
Size: 359 34 608 360 436
Fragments arranged by size:
608 436 360 359 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 670 682 1797
Size: 670 12 1115
Fragments arranged by size:
1115 670 12
BtsI GCAGTG_nn'
Cuts at: 0 901 1197 1797
Size: 901 296 600
Fragments arranged by size:
901 600 296
DraIII CAC_nnn'GTG
Cuts at: 0 306 324 1797
Size: 306 18 1473
Fragments arranged by size:
1473 306 18
EaeI y'GGCC_r
Cuts at: 0 252 864 1797
Size: 252 612 933
Fragments arranged by size:
933 612 252
EarI CTCTTCn'nnn_
Cuts at: 0 334 487 713 1139 1179 1235 1797
Size: 334 153 226 426 40 56 562
Fragments arranged by size:
562 426 334 226 153 56 40
EcoICRI GAG'CTC
Cuts at: 0 1470 1797
Size: 1470 327
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 358 661 694 1062 1486 1576 1797
Size: 2 356 303 33 368 424 90 221
Fragments arranged by size:
424 368 356 303 221 90 33 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1558 1797
Size: 1558 239
EcoO109I rG'GnC_Cy
Cuts at: 0 542 1797
Size: 542 1255
EcoRI G'AATT_C
Cuts at: 0 1691 1797
Size: 1691 106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1293 1797
Size: 1293 504
HaeII r_GCGC'y
Cuts at: 0 47 1554 1617 1797
Size: 47 1507 63 180
Fragments arranged by size:
1507 180 63 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 795 827 942 945 974 977 1008 1040
Size: 795 32 115 3 29 3 31 32
Cuts at: 1040 1053 1085 1206 1238 1339 1371 1454 1486
Size: 13 32 121 32 101 32 83 32
Cuts at: 1486 1797
Size: 311
Fragments arranged by size:
795 311 121 115 101 83 32 32
32 32 32 32 31 29 13 3
3
HincII GTy'rAC
Cuts at: 0 213 522 1376 1797
Size: 213 309 854 421
Fragments arranged by size:
854 421 309 213
HindIII A'AGCT_T
Cuts at: 0 688 1797
Size: 688 1109
HpaI GTT'AAC
Cuts at: 0 522 1797
Size: 522 1275
KasI G'GCGC_C
Cuts at: 0 43 1797
Size: 43 1754
KpnI G_GTAC'C
Cuts at: 0 1091 1249 1797
Size: 1091 158 548
Fragments arranged by size:
1091 548 158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 913 1797
Size: 658 255 884
Fragments arranged by size:
884 658 255
MscI TGG'CCA
Cuts at: 0 866 1797
Size: 866 931
MslI CAynn'nnrTG
Cuts at: 0 1006 1733 1797
Size: 1006 727 64
Fragments arranged by size:
1006 727 64
MspA1I CmG'CkG
Cuts at: 0 41 395 428 649 1293 1318 1797
Size: 41 354 33 221 644 25 479
Fragments arranged by size:
644 479 354 221 41 33 25
NaeI GCC'GGC
Cuts at: 0 48 1797
Size: 48 1749
NarI GG'CG_CC
Cuts at: 0 44 1797
Size: 44 1753
NcoI C'CATG_G
Cuts at: 0 1001 1797
Size: 1001 796
NgoMIV G'CCGG_C
Cuts at: 0 46 1797
Size: 46 1751
NspI r_CATG'y
Cuts at: 0 219 1797
Size: 219 1578
PflMI CCAn_nnn'nTGG
Cuts at: 0 877 1797
Size: 877 920
PfoI T'CCnGG_A
Cuts at: 0 1424 1797
Size: 1424 373
PsiI TTA'TAA
Cuts at: 0 1099 1797
Size: 1099 698
PstI C_TGCA'G
Cuts at: 0 654 908 1056 1797
Size: 654 254 148 741
Fragments arranged by size:
741 654 254 148
PvuII CAG'CTG
Cuts at: 0 649 1293 1797
Size: 649 644 504
Fragments arranged by size:
649 644 504
SacI G_AGCT'C
Cuts at: 0 1472 1797
Size: 1472 325
SacII CC_GC'GG
Cuts at: 0 396 1797
Size: 396 1401
SalI G'TCGA_C
Cuts at: 0 211 1797
Size: 211 1586
SexAI A'CCwGG_T
Cuts at: 0 302 1797
Size: 302 1495
SfcI C'TryA_G
Cuts at: 0 650 904 1052 1473 1797
Size: 650 254 148 421 324
Fragments arranged by size:
650 421 324 254 148
SfoI GGC'GCC
Cuts at: 0 45 1797
Size: 45 1752
SgrAI Cr'CCGG_yG
Cuts at: 0 46 1797
Size: 46 1751
SmaI CCC'GGG
Cuts at: 0 56 417 1797
Size: 56 361 1380
Fragments arranged by size:
1380 361 56
SmlI C'TyrA_G
Cuts at: 0 295 1639 1797
Size: 295 1344 158
Fragments arranged by size:
1344 295 158
SpeI A'CTAG_T
Cuts at: 0 1654 1797
Size: 1654 143
StuI AGG'CCT
Cuts at: 0 608 1797
Size: 608 1189
StyI C'CwwG_G
Cuts at: 0 1001 1277 1595 1797
Size: 1001 276 318 202
Fragments arranged by size:
1001 318 276 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 751 1797
Size: 751 1046
TatI w'GTAC_w
Cuts at: 0 83 326 367 1797
Size: 83 243 41 1430
Fragments arranged by size:
1430 243 83 41
XbaI T'CTAG_A
Cuts at: 0 1140 1797
Size: 1140 657
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 874 1797
Size: 874 923
XmaI C'CCGG_G
Cuts at: 0 54 415 1797
Size: 54 361 1382
Fragments arranged by size:
1382 361 54
XmnI GAAnn'nnTTC
Cuts at: 0 451 1797
Size: 451 1346
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AlfI AlwNI ApoI
AvaI BaeI BamHI BanI BanII BbeI BbvCI BclI
BglI BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PflMI PfoI PsiI
PstI PvuII SacI SacII SalI SexAI SfcI SfoI
SgrAI SmaI SmlI SpeI StuI StyI TaqII TatI
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflIII AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BbsI BcgI
BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BsaI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BssHII
BssSI BstEII BstZ17I ClaI CspCI DraI DrdI EagI
EciI EcoRV FseI FspI FspAI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
RsrII SanDI SapI SbfI ScaI SfiI SgfI SnaBI
SphI SrfI SspI SwaI TstI Tth111I XhoI ZraI