Restriction map for
pF1KB7822
(Linear) (Six-base) MAPSORT of: KIBB7822.seq Check: 5604 from: 1 to: 1800
KIBB7822.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1215 1800
Size: 1215 585
AatII G_ACGT'C
Cuts at: 0 179 1800
Size: 179 1621
Acc65I G'GTAC_C
Cuts at: 0 1187 1800
Size: 1187 613
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1564 1800
Size: 400 1164 236
Fragments arranged by size:
1164 400 236
AleI CACnn'nnGTG
Cuts at: 0 931 1800
Size: 931 869
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 345 1800
Size: 345 1455
AlwNI CAG_nnn'CTG
Cuts at: 0 405 420 756 1050 1800
Size: 405 15 336 294 750
Fragments arranged by size:
750 405 336 294 15
ApaLI G'TGCA_C
Cuts at: 0 1345 1800
Size: 1345 455
AvaI C'yCGr_G
Cuts at: 0 409 696 985 1103 1196 1631 1800
Size: 409 287 289 118 93 435 169
Fragments arranged by size:
435 409 289 287 169 118 93
BanI G'GyrC_C
Cuts at: 0 933 1187 1593 1800
Size: 933 254 406 207
Fragments arranged by size:
933 406 254 207
BanII G_rGCy'C
Cuts at: 0 206 847 886 1094 1165 1411 1521 1800
Size: 206 641 39 208 71 246 110 279
Fragments arranged by size:
641 279 246 208 206 110 71 39
BbvCI CC'TCA_GC
Cuts at: 0 1232 1800
Size: 1232 568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1725 1759 1800
Size: 1725 34 41
Fragments arranged by size:
1725 41 34
BglI GCCn_nnn'nGGC
Cuts at: 0 214 614 1800
Size: 214 400 1186
Fragments arranged by size:
1186 400 214
Bme1580I G_kGCm'C
Cuts at: 0 893 1349 1800
Size: 893 456 451
Fragments arranged by size:
893 456 451
BmrI ACTGGGnnnn_n'
Cuts at: 0 992 1006 1800
Size: 992 14 794
Fragments arranged by size:
992 794 14
BmtI G_CTAG'C
Cuts at: 0 506 1800
Size: 506 1294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 133 382 646 1387 1800
Size: 90 43 249 264 741 413
Fragments arranged by size:
741 413 264 249 90 43
Bpu10I CC'TnA_GC
Cuts at: 0 1232 1468 1800
Size: 1232 236 332
Fragments arranged by size:
1232 332 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1045 1800
Size: 163 882 755
Fragments arranged by size:
882 755 163
BsaI GGTCTCn'nnnn_
Cuts at: 0 498 1800
Size: 498 1302
BsaHI Gr'CG_yC
Cuts at: 0 16 176 1241 1800
Size: 16 160 1065 559
Fragments arranged by size:
1065 559 160 16
BsaWI w'CCGG_w
Cuts at: 0 74 1253 1800
Size: 74 1179 547
Fragments arranged by size:
1179 547 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 24 676 706 1345 1375 1800
Size: 24 652 30 639 30 425
Fragments arranged by size:
652 639 425 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 1116 1143 1567 1800
Size: 214 902 27 424 233
Fragments arranged by size:
902 424 233 214 27
BseYI C'CCAG_C
Cuts at: 0 429 892 1083 1800
Size: 429 463 191 717
Fragments arranged by size:
717 463 429 191
BsiEI CG_ry'CG
Cuts at: 0 337 682 1800
Size: 337 345 1118
Fragments arranged by size:
1118 345 337
BsiHKAI G_wGCw'C
Cuts at: 0 206 1349 1561 1800
Size: 206 1143 212 239
Fragments arranged by size:
1143 239 212 206
BsiWI C'GTAC_G
Cuts at: 0 975 1800
Size: 975 825
BsmBI CGTCTCn'nnnn_
Cuts at: 0 23 1800
Size: 23 1777
Bsp1286I G_dGCh'C
Cuts at: 0 206 847 886 893 1094 1165 1349 1411
Size: 206 641 39 7 201 71 184 62
Cuts at: 1411 1521 1561 1800
Size: 110 40 239
Fragments arranged by size:
641 239 206 201 184 110 71 62
40 39 7
BspEI T'CCGG_A
Cuts at: 0 74 1800
Size: 74 1726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1215 1800
Size: 1215 585
BsrBI CCG'CTC
Cuts at: 0 1266 1800
Size: 1266 534
BsrDI GCAATG_nn'
Cuts at: 0 396 1800
Size: 396 1404
BsrFI r'CCGG_y
Cuts at: 0 38 255 301 332 1472 1800
Size: 38 217 46 31 1140 328
Fragments arranged by size:
1140 328 217 46 38 31
BssHII G'CGCG_C
Cuts at: 0 1502 1800
Size: 1502 298
BstAPI GCAn_nnn'nTGC
Cuts at: 0 922 1800
Size: 922 878
BstBI TT'CG_AA
Cuts at: 0 1415 1800
Size: 1415 385
BstEII G'GTnAC_C
Cuts at: 0 451 1800
Size: 451 1349
BstXI CCAn_nnnn'nTGG
Cuts at: 0 585 1019 1800
Size: 585 434 781
Fragments arranged by size:
781 585 434
BstYI r'GATC_y
Cuts at: 0 659 1800
Size: 659 1141
Bsu36I CC'TnA_GG
Cuts at: 0 1155 1800
Size: 1155 645
BtgI C'CryG_G
Cuts at: 0 904 1610 1800
Size: 904 706 190
Fragments arranged by size:
904 706 190
BtsI GCAGTG_nn'
Cuts at: 0 600 1738 1800
Size: 600 1138 62
Fragments arranged by size:
1138 600 62
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 633 668 1800
Size: 633 35 1132
Fragments arranged by size:
1132 633 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 517 1800
Size: 517 1283
EaeI y'GGCC_r
Cuts at: 0 334 468 1678 1800
Size: 334 134 1210 122
Fragments arranged by size:
1210 334 134 122
EagI C'GGCC_G
Cuts at: 0 334 1800
Size: 334 1466
EarI CTCTTCn'nnn_
Cuts at: 0 376 1177 1800
Size: 376 801 623
Fragments arranged by size:
801 623 376
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1150 1800
Size: 1150 650
EcoICRI GAG'CTC
Cuts at: 0 204 1800
Size: 204 1596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 133 382 400 646 1387 1564 1800
Size: 90 43 249 18 246 741 177 236
Fragments arranged by size:
741 249 246 236 177 90 43 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1546 1800
Size: 1546 254
EcoO109I rG'GnC_Cy
Cuts at: 0 781 799 1800
Size: 781 18 1001
Fragments arranged by size:
1001 781 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 157 1293 1800
Size: 157 1136 507
Fragments arranged by size:
1136 507 157
HaeII r_GCGC'y
Cuts at: 0 280 447 1770 1800
Size: 280 167 1323 30
Fragments arranged by size:
1323 280 167 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 1135 1167 1290 1322 1345 1377 1800
Size: 26 1109 32 123 32 23 32 423
Fragments arranged by size:
1109 423 123 32 32 32 26 23
HincII GTy'rAC
Cuts at: 0 669 1212 1800
Size: 669 543 588
Fragments arranged by size:
669 588 543
HpaI GTT'AAC
Cuts at: 0 1212 1800
Size: 1212 588
KpnI G_GTAC'C
Cuts at: 0 1191 1800
Size: 1191 609
MslI CAynn'nnrTG
Cuts at: 0 266 931 1800
Size: 266 665 869
Fragments arranged by size:
869 665 266
MspA1I CmG'CkG
Cuts at: 0 7 417 756 906 1047 1581 1800
Size: 7 410 339 150 141 534 219
Fragments arranged by size:
534 410 339 219 150 141 7
NaeI GCC'GGC
Cuts at: 0 257 334 1474 1800
Size: 257 77 1140 326
Fragments arranged by size:
1140 326 257 77
NgoMIV G'CCGG_C
Cuts at: 0 255 332 1472 1800
Size: 255 77 1140 328
Fragments arranged by size:
1140 328 255 77
NheI G'CTAG_C
Cuts at: 0 502 1800
Size: 502 1298
NspI r_CATG'y
Cuts at: 0 604 1588 1800
Size: 604 984 212
Fragments arranged by size:
984 604 212
PasI CC'CwG_GG
Cuts at: 0 475 1513 1800
Size: 475 1038 287
Fragments arranged by size:
1038 475 287
PflMI CCAn_nnn'nTGG
Cuts at: 0 1050 1676 1800
Size: 1050 626 124
Fragments arranged by size:
1050 626 124
PfoI T'CCnGG_A
Cuts at: 0 1363 1800
Size: 1363 437
PpuMI rG'GwC_Cy
Cuts at: 0 799 1800
Size: 799 1001
PspXI vC'TCGA_Gb
Cuts at: 0 985 1800
Size: 985 815
PstI C_TGCA'G
Cuts at: 0 50 752 1800
Size: 50 702 1048
Fragments arranged by size:
1048 702 50
PvuII CAG'CTG
Cuts at: 0 756 1581 1800
Size: 756 825 219
Fragments arranged by size:
825 756 219
SacI G_AGCT'C
Cuts at: 0 206 1800
Size: 206 1594
SacII CC_GC'GG
Cuts at: 0 907 1800
Size: 907 893
SanDI GG'GwC_CC
Cuts at: 0 799 1800
Size: 799 1001
SapI GCTCTTCn'nnn_
Cuts at: 0 376 1800
Size: 376 1424
SfcI C'TryA_G
Cuts at: 0 46 748 1800
Size: 46 702 1052
Fragments arranged by size:
1052 702 46
SmaI CCC'GGG
Cuts at: 0 411 698 1105 1633 1800
Size: 411 287 407 528 167
Fragments arranged by size:
528 411 407 287 167
SmlI C'TyrA_G
Cuts at: 0 142 985 1024 1196 1800
Size: 142 843 39 172 604
Fragments arranged by size:
843 604 172 142 39
SphI G_CATG'C
Cuts at: 0 604 1800
Size: 604 1196
SrfI GCCC'GGGC
Cuts at: 0 411 1800
Size: 411 1389
StyI C'CwwG_G
Cuts at: 0 488 794 878 1130 1391 1800
Size: 488 306 84 252 261 409
Fragments arranged by size:
488 409 306 261 252 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 375 488 501 891 1800
Size: 375 113 13 390 909
Fragments arranged by size:
909 390 375 113 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 676 708 1800
Size: 676 32 1092
Fragments arranged by size:
1092 676 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1371 1800
Size: 1371 429
XhoI C'TCGA_G
Cuts at: 0 985 1196 1800
Size: 985 211 604
Fragments arranged by size:
985 604 211
XmaI C'CCGG_G
Cuts at: 0 409 696 1103 1631 1800
Size: 409 287 407 528 169
Fragments arranged by size:
528 409 407 287 169
ZraI GAC'GTC
Cuts at: 0 177 1800
Size: 177 1623
Enzymes that do cut:
AarI AatII Acc65I AcuI AleI AlfI AlwNI ApaLI
AvaI BanI BanII BbvCI BcgI BglI Bme1580I BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII HpaI KpnI MslI MspA1I NaeI
NgoMIV NheI NspI PasI PflMI PfoI PpuMI PspXI
PstI PvuII SacI SacII SanDI SapI SfcI SmaI
SmlI SphI SrfI StyI TaqII TstI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AloI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BciVI BclI BfrBI BglII BlpI BmgBI
BplI BsaAI BsaBI BsgI BsmI BspHI BsrGI BssSI
BstZ17I BtgZI ClaI DraI DraIII EcoRI EcoRV FseI
FspI FspAI HindIII KasI MfeI MluI MmeI MscI
NarI NcoI NdeI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI
RsrII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SspI StuI SwaI TatI Tth111I
XbaI XmnI