Restriction map for
pF1KB7823
(Linear) (Six-base) MAPSORT of: KIBB7823.seq Check: 6238 from: 1 to: 1608
KIBB7823.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1258 1608
Size: 1258 350
AccI GT'mk_AC
Cuts at: 0 1449 1608
Size: 1449 159
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 296 1123 1608
Size: 80 216 827 485
Fragments arranged by size:
827 485 216 80
AflIII A'CryG_T
Cuts at: 0 1370 1608
Size: 1370 238
AlwNI CAG_nnn'CTG
Cuts at: 0 1120 1608
Size: 1120 488
ApoI r'AATT_y
Cuts at: 0 650 846 1283 1608
Size: 650 196 437 325
Fragments arranged by size:
650 437 325 196
AseI AT'TA_AT
Cuts at: 0 641 1608
Size: 641 967
AvaI C'yCGr_G
Cuts at: 0 656 1608
Size: 656 952
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 142 175 1608
Size: 142 33 1433
Fragments arranged by size:
1433 142 33
BciVI GTATCCnnnnn_n'
Cuts at: 0 127 143 224 1608
Size: 127 16 81 1384
Fragments arranged by size:
1384 127 81 16
BclI T'GATC_A
Cuts at: 0 1299 1608
Size: 1299 309
BfrBI ATG'CAT
Cuts at: 0 690 1608
Size: 690 918
BglI GCCn_nnn'nGGC
Cuts at: 0 859 1608
Size: 859 749
Bme1580I G_kGCm'C
Cuts at: 0 918 1608
Size: 918 690
BmrI ACTGGGnnnn_n'
Cuts at: 0 204 1343 1608
Size: 204 1139 265
Fragments arranged by size:
1139 265 204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 1329 1608
Size: 590 739 279
Fragments arranged by size:
739 590 279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 1608
Size: 73 1535
BsaI GGTCTCn'nnnn_
Cuts at: 0 108 1608
Size: 108 1500
BsaAI yAC'GTr
Cuts at: 0 1048 1608
Size: 1048 560
BsaWI w'CCGG_w
Cuts at: 0 137 1097 1608
Size: 137 960 511
Fragments arranged by size:
960 511 137
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1302 1332 1608
Size: 1302 30 276
Fragments arranged by size:
1302 276 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 96 662 1608
Size: 96 566 946
Fragments arranged by size:
946 566 96
BseYI C'CCAG_C
Cuts at: 0 1006 1608
Size: 1006 602
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 1608
Size: 8 1600
BsiWI C'GTAC_G
Cuts at: 0 128 1608
Size: 128 1480
BsmI GAATG_Cn'
Cuts at: 0 255 381 559 690 1608
Size: 255 126 178 131 918
Fragments arranged by size:
918 255 178 131 126
Bsp1286I G_dGCh'C
Cuts at: 0 918 1608
Size: 918 690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 30 401 1258 1608
Size: 30 371 857 350
Fragments arranged by size:
857 371 350 30
BsrDI GCAATG_nn'
Cuts at: 0 875 1608
Size: 875 733
BssSI C'ACGA_G
Cuts at: 0 893 1576 1608
Size: 893 683 32
Fragments arranged by size:
893 683 32
BstXI CCAn_nnnn'nTGG
Cuts at: 0 267 1251 1608
Size: 267 984 357
Fragments arranged by size:
984 357 267
BstYI r'GATC_y
Cuts at: 0 73 197 612 1488 1608
Size: 73 124 415 876 120
Fragments arranged by size:
876 415 124 120 73
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 712 747 1608
Size: 712 35 861
Fragments arranged by size:
861 712 35
EaeI y'GGCC_r
Cuts at: 0 257 1608
Size: 257 1351
EarI CTCTTCn'nnn_
Cuts at: 0 108 810 956 996 1237 1588 1608
Size: 108 702 146 40 241 351 20
Fragments arranged by size:
702 351 241 146 108 40 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 296 590 1123 1329 1608
Size: 80 216 294 533 206 279
Fragments arranged by size:
533 294 279 216 206 80
EcoRV GAT'ATC
Cuts at: 0 1161 1608
Size: 1161 447
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 985 1608
Size: 985 623
HaeII r_GCGC'y
Cuts at: 0 7 1608
Size: 7 1601
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 995 1002 1027 1034 1114 1146 1608
Size: 995 7 25 7 80 32 462
Fragments arranged by size:
995 462 80 32 25 7 7
MfeI C'AATT_G
Cuts at: 0 299 1608
Size: 299 1309
MscI TGG'CCA
Cuts at: 0 259 1608
Size: 259 1349
MslI CAynn'nnrTG
Cuts at: 0 265 839 1580 1608
Size: 265 574 741 28
Fragments arranged by size:
741 574 265 28
MspA1I CmG'CkG
Cuts at: 0 502 1608
Size: 502 1106
NsiI A_TGCA'T
Cuts at: 0 692 1608
Size: 692 916
NspI r_CATG'y
Cuts at: 0 1374 1608
Size: 1374 234
PciI A'CATG_T
Cuts at: 0 1370 1608
Size: 1370 238
PmlI CAC'GTG
Cuts at: 0 1048 1608
Size: 1048 560
PshAI GACnn'nnGTC
Cuts at: 0 797 1608
Size: 797 811
PsiI TTA'TAA
Cuts at: 0 1289 1608
Size: 1289 319
PvuII CAG'CTG
Cuts at: 0 502 1608
Size: 502 1106
SapI GCTCTTCn'nnn_
Cuts at: 0 996 1237 1608
Size: 996 241 371
Fragments arranged by size:
996 371 241
SexAI A'CCwGG_T
Cuts at: 0 353 1608
Size: 353 1255
SfcI C'TryA_G
Cuts at: 0 823 1608
Size: 823 785
SmaI CCC'GGG
Cuts at: 0 658 1608
Size: 658 950
SmlI C'TyrA_G
Cuts at: 0 52 1608
Size: 52 1556
StyI C'CwwG_G
Cuts at: 0 628 1244 1568 1608
Size: 628 616 324 40
Fragments arranged by size:
628 616 324 40
TatI w'GTAC_w
Cuts at: 0 831 1608
Size: 831 777
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 879 911 1608
Size: 879 32 697
Fragments arranged by size:
879 697 32
Tth111I GACn'n_nGTC
Cuts at: 0 328 1608
Size: 328 1280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1356 1608
Size: 1356 252
XmaI C'CCGG_G
Cuts at: 0 656 1608
Size: 656 952
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApoI AseI AvaI
BaeI BciVI BclI BfrBI BglI Bme1580I BmrI BpmI
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiWI BsmI Bsp1286I BspMI BsrDI BssSI BstXI BstYI
CspCI EaeI EarI Eco57MI EcoRV FalI HaeII Hin4I
MfeI MscI MslI MspA1I NsiI NspI PciI PmlI
PshAI PsiI PvuII SapI SexAI SfcI SmaI SmlI
StyI TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BglII BlpI
BmgBI BmtI BplI Bpu10I BsaBI BsaHI BsiEI BsiHKAI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PflMI PfoI PmeI
PpiI PpuMI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII XbaI XhoI XmnI ZraI