Restriction map for
pF1KB7826
(Linear) (Six-base) MAPSORT of: KIBB7826.seq Check: 1975 from: 1 to: 1812
KIBB7826.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AclI AA'CG_TT
Cuts at: 0 743 1812
Size: 743 1069
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 900 1320 1812
Size: 417 483 420 492
Fragments arranged by size:
492 483 420 417
AflIII A'CryG_T
Cuts at: 0 817 1812
Size: 817 995
AleI CACnn'nnGTG
Cuts at: 0 1295 1812
Size: 1295 517
AlwNI CAG_nnn'CTG
Cuts at: 0 469 1812
Size: 469 1343
ApoI r'AATT_y
Cuts at: 0 866 1286 1538 1622 1790 1812
Size: 866 420 252 84 168 22
Fragments arranged by size:
866 420 252 168 84 22
BanII G_rGCy'C
Cuts at: 0 680 1206 1812
Size: 680 526 606
Fragments arranged by size:
680 606 526
BbsI GAAGACnn'nnnn_
Cuts at: 0 1351 1812
Size: 1351 461
BciVI GTATCCnnnnn_n'
Cuts at: 0 155 1812
Size: 155 1657
BclI T'GATC_A
Cuts at: 0 636 1812
Size: 636 1176
BmrI ACTGGGnnnn_n'
Cuts at: 0 285 508 968 1052 1136 1388 1724 1812
Size: 285 223 460 84 84 252 336 88
Fragments arranged by size:
460 336 285 252 223 88 84 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1232 1484 1812
Size: 133 1099 252 328
Fragments arranged by size:
1099 328 252 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 1812
Size: 712 1100
BsaI GGTCTCn'nnnn_
Cuts at: 0 138 1812
Size: 138 1674
BsaBI GATnn'nnATC
Cuts at: 0 242 728 1812
Size: 242 486 1084
Fragments arranged by size:
1084 486 242
BsaHI Gr'CG_yC
Cuts at: 0 341 1812
Size: 341 1471
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 1812
Size: 73 1739
BsiHKAI G_wGCw'C
Cuts at: 0 367 1206 1320 1812
Size: 367 839 114 492
Fragments arranged by size:
839 492 367 114
BsmI GAATG_Cn'
Cuts at: 0 1075 1812
Size: 1075 737
Bsp1286I G_dGCh'C
Cuts at: 0 367 680 1206 1320 1812
Size: 367 313 526 114 492
Fragments arranged by size:
526 492 367 313 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 429 1812
Size: 429 1383
BsrGI T'GTAC_A
Cuts at: 0 95 855 939 1779 1812
Size: 95 760 84 840 33
Fragments arranged by size:
840 760 95 84 33
BstYI r'GATC_y
Cuts at: 0 227 1812
Size: 227 1585
BtgI C'CryG_G
Cuts at: 0 196 1812
Size: 196 1616
DraI TTT'AAA
Cuts at: 0 374 570 1812
Size: 374 196 1242
Fragments arranged by size:
1242 374 196
DraIII CAC_nnn'GTG
Cuts at: 0 1800 1812
Size: 1800 12
EarI CTCTTCn'nnn_
Cuts at: 0 185 265 436 1812
Size: 185 80 171 1376
Fragments arranged by size:
1376 185 171 80
EcoICRI GAG'CTC
Cuts at: 0 1204 1812
Size: 1204 608
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 417 900 1232 1320 1484 1812
Size: 133 284 483 332 88 164 328
Fragments arranged by size:
483 332 328 284 164 133 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1338 1812
Size: 1338 474
EcoO109I rG'GnC_Cy
Cuts at: 0 89 1812
Size: 89 1723
EcoRI G'AATT_C
Cuts at: 0 866 1286 1538 1622 1790 1812
Size: 866 420 252 84 168 22
Fragments arranged by size:
866 420 252 168 84 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 154 186 1812
Size: 154 32 1626
Fragments arranged by size:
1626 154 32
HincII GTy'rAC
Cuts at: 0 453 1812
Size: 453 1359
HpaI GTT'AAC
Cuts at: 0 453 1812
Size: 453 1359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 1812
Size: 158 1654
MslI CAynn'nnrTG
Cuts at: 0 557 1295 1812
Size: 557 738 517
Fragments arranged by size:
738 557 517
MspA1I CmG'CkG
Cuts at: 0 399 1185 1812
Size: 399 786 627
Fragments arranged by size:
786 627 399
NcoI C'CATG_G
Cuts at: 0 196 1812
Size: 196 1616
NdeI CA'TA_TG
Cuts at: 0 888 1648 1812
Size: 888 760 164
Fragments arranged by size:
888 760 164
NspI r_CATG'y
Cuts at: 0 821 905 1812
Size: 821 84 907
Fragments arranged by size:
907 821 84
PasI CC'CwG_GG
Cuts at: 0 12 1812
Size: 12 1800
PciI A'CATG_T
Cuts at: 0 817 1812
Size: 817 995
PfoI T'CCnGG_A
Cuts at: 0 1608 1812
Size: 1608 204
PpuMI rG'GwC_Cy
Cuts at: 0 89 1812
Size: 89 1723
PsiI TTA'TAA
Cuts at: 0 611 1812
Size: 611 1201
PstI C_TGCA'G
Cuts at: 0 404 1812
Size: 404 1408
PvuII CAG'CTG
Cuts at: 0 399 1185 1812
Size: 399 786 627
Fragments arranged by size:
786 627 399
SacI G_AGCT'C
Cuts at: 0 1206 1812
Size: 1206 606
SapI GCTCTTCn'nnn_
Cuts at: 0 185 1812
Size: 185 1627
SexAI A'CCwGG_T
Cuts at: 0 128 1812
Size: 128 1684
SfcI C'TryA_G
Cuts at: 0 120 400 1812
Size: 120 280 1412
Fragments arranged by size:
1412 280 120
SmlI C'TyrA_G
Cuts at: 0 691 1812
Size: 691 1121
SphI G_CATG'C
Cuts at: 0 905 1812
Size: 905 907
SspI AAT'ATT
Cuts at: 0 509 591 1812
Size: 509 82 1221
Fragments arranged by size:
1221 509 82
StuI AGG'CCT
Cuts at: 0 1165 1669 1753 1812
Size: 1165 504 84 59
Fragments arranged by size:
1165 504 84 59
StyI C'CwwG_G
Cuts at: 0 196 1812
Size: 196 1616
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 1812
Size: 67 1745
TatI w'GTAC_w
Cuts at: 0 95 855 939 1399 1567 1779 1812
Size: 95 760 84 460 168 212 33
Fragments arranged by size:
760 460 212 168 95 84 33
XbaI T'CTAG_A
Cuts at: 0 411 1812
Size: 411 1401
XmnI GAAnn'nnTTC
Cuts at: 0 1081 1249 1585 1753 1812
Size: 1081 168 336 168 59
Fragments arranged by size:
1081 336 168 168 59
Enzymes that do cut:
AclI AcuI AflIII AleI AlwNI ApoI BanII BbsI
BciVI BclI BmrI BpmI BpuEI BsaI BsaBI BsaHI
BseRI BsiHKAI BsmI Bsp1286I BspMI BsrGI BstYI BtgI
DraI DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
Hin4I HincII HpaI MmeI MslI MspA1I NcoI NdeI
NspI PasI PciI PfoI PpuMI PsiI PstI PvuII
SacI SapI SexAI SfcI SmlI SphI SspI StuI
StyI TaqII TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I
BsaAI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DrdI EaeI EagI EciI EcoRV FalI FseI
FspI FspAI HaeII HindIII KasI KpnI MfeI MluI
MscI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SwaI TstI Tth111I XcmI XhoI XmaI ZraI