Restriction map for
pF1KB7828
(Linear) (Six-base) MAPSORT of: KIBB7828.seq Check: 2540 from: 1 to: 1815
KIBB7828.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 912 1815
Size: 912 903
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 744 1148 1815
Size: 99 645 404 667
Fragments arranged by size:
667 645 404 99
AleI CACnn'nnGTG
Cuts at: 0 1246 1815
Size: 1246 569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 910 1264 1815
Size: 910 354 551
Fragments arranged by size:
910 551 354
ApaI G_GGCC'C
Cuts at: 0 947 1815
Size: 947 868
ApoI r'AATT_y
Cuts at: 0 244 813 850 1012 1815
Size: 244 569 37 162 803
Fragments arranged by size:
803 569 244 162 37
AseI AT'TA_AT
Cuts at: 0 1489 1815
Size: 1489 326
BanII G_rGCy'C
Cuts at: 0 460 878 947 1815
Size: 460 418 69 868
Fragments arranged by size:
868 460 418 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 456 788 1815
Size: 456 332 1027
Fragments arranged by size:
1027 456 332
BglII A'GATC_T
Cuts at: 0 84 1815
Size: 84 1731
BlpI GC'TnA_GC
Cuts at: 0 872 1815
Size: 872 943
Bme1580I G_kGCm'C
Cuts at: 0 947 1152 1815
Size: 947 205 663
Fragments arranged by size:
947 663 205
BmrI ACTGGGnnnn_n'
Cuts at: 0 454 872 1049 1815
Size: 454 418 177 766
Fragments arranged by size:
766 454 418 177
Bpu10I CC'TnA_GC
Cuts at: 0 509 1815
Size: 509 1306
BsaAI yAC'GTr
Cuts at: 0 1248 1504 1705 1815
Size: 1248 256 201 110
Fragments arranged by size:
1248 256 201 110
BsaBI GATnn'nnATC
Cuts at: 0 83 1586 1815
Size: 83 1503 229
Fragments arranged by size:
1503 229 83
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 580 1815
Size: 580 1235
BseYI C'CCAG_C
Cuts at: 0 38 266 1815
Size: 38 228 1549
Fragments arranged by size:
1549 228 38
BsiHKAI G_wGCw'C
Cuts at: 0 1243 1815
Size: 1243 572
Bsp1286I G_dGCh'C
Cuts at: 0 460 878 947 1152 1243 1815
Size: 460 418 69 205 91 572
Fragments arranged by size:
572 460 418 205 91 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 912 1815
Size: 912 903
BsrDI GCAATG_nn'
Cuts at: 0 467 1773 1815
Size: 467 1306 42
Fragments arranged by size:
1306 467 42
BstYI r'GATC_y
Cuts at: 0 84 292 1815
Size: 84 208 1523
Fragments arranged by size:
1523 208 84
BtgI C'CryG_G
Cuts at: 0 632 1815
Size: 632 1183
DraIII CAC_nnn'GTG
Cuts at: 0 1080 1815
Size: 1080 735
EarI CTCTTCn'nnn_
Cuts at: 0 227 1130 1815
Size: 227 903 685
Fragments arranged by size:
903 685 227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 744 1148 1815
Size: 99 645 404 667
Fragments arranged by size:
667 645 404 99
EcoRI G'AATT_C
Cuts at: 0 1012 1815
Size: 1012 803
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1498 1815
Size: 1498 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 559 591 1012 1044 1815
Size: 559 32 421 32 771
Fragments arranged by size:
771 559 421 32 32
HincII GTy'rAC
Cuts at: 0 669 797 831 1437 1815
Size: 669 128 34 606 378
Fragments arranged by size:
669 606 378 128 34
HindIII A'AGCT_T
Cuts at: 0 977 1815
Size: 977 838
MslI CAynn'nnrTG
Cuts at: 0 1246 1250 1815
Size: 1246 4 565
Fragments arranged by size:
1246 565 4
NcoI C'CATG_G
Cuts at: 0 632 1815
Size: 632 1183
NspI r_CATG'y
Cuts at: 0 360 897 1701 1815
Size: 360 537 804 114
Fragments arranged by size:
804 537 360 114
PmlI CAC'GTG
Cuts at: 0 1248 1815
Size: 1248 567
PshAI GACnn'nnGTC
Cuts at: 0 33 116 1815
Size: 33 83 1699
Fragments arranged by size:
1699 83 33
PspOMI G'GGCC_C
Cuts at: 0 943 1815
Size: 943 872
PstI C_TGCA'G
Cuts at: 0 1751 1815
Size: 1751 64
SapI GCTCTTCn'nnn_
Cuts at: 0 1130 1815
Size: 1130 685
SfcI C'TryA_G
Cuts at: 0 723 1406 1747 1815
Size: 723 683 341 68
Fragments arranged by size:
723 683 341 68
SnaBI TAC'GTA
Cuts at: 0 1504 1815
Size: 1504 311
SphI G_CATG'C
Cuts at: 0 360 897 1701 1815
Size: 360 537 804 114
Fragments arranged by size:
804 537 360 114
SspI AAT'ATT
Cuts at: 0 603 1815
Size: 603 1212
StyI C'CwwG_G
Cuts at: 0 632 1815
Size: 632 1183
TatI w'GTAC_w
Cuts at: 0 341 1188 1815
Size: 341 847 627
Fragments arranged by size:
847 627 341
Enzymes that do cut:
AarI AcuI AleI AlfI ApaI ApoI AseI BanII
BbsI BglII BlpI Bme1580I BmrI Bpu10I BsaAI BsaBI
BseRI BseYI BsiHKAI Bsp1286I BspMI BsrDI BstYI BtgI
DraIII EarI Eco57MI EcoRI FalI Hin4I HincII HindIII
MslI NcoI NspI PmlI PshAI PspOMI PstI SapI
SfcI SnaBI SphI SspI StyI TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BmgBI BmtI BplI BpmI BpuEI BsaI
BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI
EcoO109I EcoRV FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SrfI StuI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI