Restriction map for
pF1KB7833
(Linear) (Six-base) MAPSORT of: KIBB7833.seq Check: 4393 from: 1 to: 1614
KIBB7833.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 450 1404 1614
Size: 366 84 954 210
Fragments arranged by size:
954 366 210 84
AflII C'TTAA_G
Cuts at: 0 316 1614
Size: 316 1298
AleI CACnn'nnGTG
Cuts at: 0 1127 1463 1614
Size: 1127 336 151
Fragments arranged by size:
1127 336 151
AlwNI CAG_nnn'CTG
Cuts at: 0 566 1614
Size: 566 1048
ApoI r'AATT_y
Cuts at: 0 204 614 715 817 913 1614
Size: 204 410 101 102 96 701
Fragments arranged by size:
701 410 204 102 101 96
AseI AT'TA_AT
Cuts at: 0 176 1172 1614
Size: 176 996 442
Fragments arranged by size:
996 442 176
BbvCI CC'TCA_GC
Cuts at: 0 197 1614
Size: 197 1417
BglII A'GATC_T
Cuts at: 0 846 1614
Size: 846 768
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 980 1064 1614
Size: 151 829 84 550
Fragments arranged by size:
829 550 151 84
Bpu10I CC'TnA_GC
Cuts at: 0 197 1614
Size: 197 1417
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 524 686 1614
Size: 318 206 162 928
Fragments arranged by size:
928 318 206 162
BsaI GGTCTCn'nnnn_
Cuts at: 0 624 1614
Size: 624 990
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 579 1335 1614
Size: 91 488 756 279
Fragments arranged by size:
756 488 279 91
BseYI C'CCAG_C
Cuts at: 0 7 1614
Size: 7 1607
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1316 1614
Size: 1316 298
BsrGI T'GTAC_A
Cuts at: 0 687 1107 1614
Size: 687 420 507
Fragments arranged by size:
687 507 420
BstXI CCAn_nnnn'nTGG
Cuts at: 0 234 1614
Size: 234 1380
BstYI r'GATC_y
Cuts at: 0 846 1614
Size: 846 768
BtsI GCAGTG_nn'
Cuts at: 0 567 1614
Size: 567 1047
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 969 1004 1614
Size: 969 35 610
Fragments arranged by size:
969 610 35
DraI TTT'AAA
Cuts at: 0 1188 1614
Size: 1188 426
EarI CTCTTCn'nnn_
Cuts at: 0 1098 1185 1614
Size: 1098 87 429
Fragments arranged by size:
1098 429 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 366 450 980 1064 1404 1614
Size: 151 215 84 530 84 340 210
Fragments arranged by size:
530 340 215 210 151 84 84
EcoRV GAT'ATC
Cuts at: 0 423 1614
Size: 423 1191
HincII GTy'rAC
Cuts at: 0 17 1234 1360 1614
Size: 17 1217 126 254
Fragments arranged by size:
1217 254 126 17
HindIII A'AGCT_T
Cuts at: 0 1079 1614
Size: 1079 535
MslI CAynn'nnrTG
Cuts at: 0 555 1127 1211 1395 1463 1614
Size: 555 572 84 184 68 151
Fragments arranged by size:
572 555 184 151 84 68
NdeI CA'TA_TG
Cuts at: 0 1112 1614
Size: 1112 502
NspI r_CATG'y
Cuts at: 0 696 737 1200 1614
Size: 696 41 463 414
Fragments arranged by size:
696 463 414 41
PsiI TTA'TAA
Cuts at: 0 1021 1614
Size: 1021 593
SfcI C'TryA_G
Cuts at: 0 674 803 1614
Size: 674 129 811
Fragments arranged by size:
811 674 129
SmlI C'TyrA_G
Cuts at: 0 297 316 539 701 1614
Size: 297 19 223 162 913
Fragments arranged by size:
913 297 223 162 19
StuI AGG'CCT
Cuts at: 0 1305 1436 1614
Size: 1305 131 178
Fragments arranged by size:
1305 178 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 85 1499 1614
Size: 85 1414 115
Fragments arranged by size:
1414 115 85
TatI w'GTAC_w
Cuts at: 0 687 1107 1443 1614
Size: 687 420 336 171
Fragments arranged by size:
687 420 336 171
XmnI GAAnn'nnTTC
Cuts at: 0 745 997 1249 1614
Size: 745 252 252 365
Fragments arranged by size:
745 365 252 252
Enzymes that do cut:
AcuI AflII AleI AlwNI ApoI AseI BbvCI BglII
BpmI Bpu10I BpuEI BsaI BseRI BseYI BsgI BsrGI
BstXI BstYI BtsI CspCI DraI EarI Eco57MI EcoRV
HincII HindIII MslI NdeI NspI PsiI SfcI SmlI
StuI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BmtI BplI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI FalI FseI
FspI FspAI HaeII Hin4I HpaI KasI KpnI MfeI
MluI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI