Restriction map for
pF1KB7834
(Linear) (Six-base) MAPSORT of: KIBB7834.seq Check: 9360 from: 1 to: 1743
KIBB7834.orf
With 182 enzymes: SgfI *
February 11, 2011 12:27 ..
AatII G_ACGT'C
Cuts at: 0 1002 1743
Size: 1002 741
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 845 998 1579 1743
Size: 496 349 153 581 164
Fragments arranged by size:
581 496 349 164 153
AflIII A'CryG_T
Cuts at: 0 467 1743
Size: 467 1276
AhdI GACnn_n'nnGTC
Cuts at: 0 236 1743
Size: 236 1507
AlwNI CAG_nnn'CTG
Cuts at: 0 310 1475 1647 1743
Size: 310 1165 172 96
Fragments arranged by size:
1165 310 172 96
ApoI r'AATT_y
Cuts at: 0 723 1015 1302 1743
Size: 723 292 287 441
Fragments arranged by size:
723 441 292 287
AvaI C'yCGr_G
Cuts at: 0 70 119 854 1743
Size: 70 49 735 889
Fragments arranged by size:
889 735 70 49
BamHI G'GATC_C
Cuts at: 0 1704 1743
Size: 1704 39
BanI G'GyrC_C
Cuts at: 0 663 1743
Size: 663 1080
BanII G_rGCy'C
Cuts at: 0 1229 1743
Size: 1229 514
BbvCI CC'TCA_GC
Cuts at: 0 135 1743
Size: 135 1608
BciVI GTATCCnnnnn_n'
Cuts at: 0 1342 1743
Size: 1342 401
BclI T'GATC_A
Cuts at: 0 357 1743
Size: 357 1386
BglI GCCn_nnn'nGGC
Cuts at: 0 1672 1743
Size: 1672 71
BglII A'GATC_T
Cuts at: 0 66 1154 1446 1743
Size: 66 1088 292 297
Fragments arranged by size:
1088 297 292 66
BlpI GC'TnA_GC
Cuts at: 0 145 1743
Size: 145 1598
Bme1580I G_kGCm'C
Cuts at: 0 1741 1743
Size: 1741 2
BmrI ACTGGGnnnn_n'
Cuts at: 0 250 1743
Size: 250 1493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 1743
Size: 411 1332
Bpu10I CC'TnA_GC
Cuts at: 0 135 1743
Size: 135 1608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 1743
Size: 143 1600
BsaBI GATnn'nnATC
Cuts at: 0 1528 1743
Size: 1528 215
BsaHI Gr'CG_yC
Cuts at: 0 999 1743
Size: 999 744
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1309 1339 1743
Size: 1309 30 404
Fragments arranged by size:
1309 404 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 123 217 1743
Size: 123 94 1526
Fragments arranged by size:
1526 123 94
BseYI C'CCAG_C
Cuts at: 0 993 1708 1743
Size: 993 715 35
Fragments arranged by size:
993 715 35
BsiHKAI G_wGCw'C
Cuts at: 0 77 849 1743
Size: 77 772 894
Fragments arranged by size:
894 772 77
BsmI GAATG_Cn'
Cuts at: 0 24 661 1743
Size: 24 637 1082
Fragments arranged by size:
1082 637 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1006 1329 1543 1743
Size: 1006 323 214 200
Fragments arranged by size:
1006 323 214 200
Bsp1286I G_dGCh'C
Cuts at: 0 77 849 1229 1741 1743
Size: 77 772 380 512 2
Fragments arranged by size:
772 512 380 77 2
BspHI T'CATG_A
Cuts at: 0 360 1743
Size: 360 1383
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1508 1743
Size: 1508 235
BsrBI CCG'CTC
Cuts at: 0 131 1743
Size: 131 1612
BsrDI GCAATG_nn'
Cuts at: 0 455 1743
Size: 455 1288
BsrFI r'CCGG_y
Cuts at: 0 1055 1743
Size: 1055 688
BstBI TT'CG_AA
Cuts at: 0 177 1743
Size: 177 1566
BstYI r'GATC_y
Cuts at: 0 66 831 1154 1446 1704 1743
Size: 66 765 323 292 258 39
Fragments arranged by size:
765 323 292 258 66 39
BtgI C'CryG_G
Cuts at: 0 1700 1743
Size: 1700 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 487 1743
Size: 487 1256
BtsI GCAGTG_nn'
Cuts at: 0 495 1743
Size: 495 1248
DraI TTT'AAA
Cuts at: 0 1025 1634 1743
Size: 1025 609 109
Fragments arranged by size:
1025 609 109
DraIII CAC_nnn'GTG
Cuts at: 0 1093 1743
Size: 1093 650
EaeI y'GGCC_r
Cuts at: 0 936 1064 1181 1743
Size: 936 128 117 562
Fragments arranged by size:
936 562 128 117
EarI CTCTTCn'nnn_
Cuts at: 0 7 219 284 837 1030 1743
Size: 7 212 65 553 193 713
Fragments arranged by size:
713 553 212 193 65 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1042 1270 1743
Size: 1042 228 473
Fragments arranged by size:
1042 473 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 496 845 998 1579 1743
Size: 411 85 349 153 581 164
Fragments arranged by size:
581 411 349 164 153 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 138 560 1743
Size: 138 422 1183
Fragments arranged by size:
1183 422 138
EcoO109I rG'GnC_Cy
Cuts at: 0 1644 1743
Size: 1644 99
EcoRI G'AATT_C
Cuts at: 0 1015 1302 1743
Size: 1015 287 441
Fragments arranged by size:
1015 441 287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1504 1743
Size: 1504 239
HaeII r_GCGC'y
Cuts at: 0 516 1743
Size: 516 1227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 222 254 1743
Size: 88 32 102 32 1489
Fragments arranged by size:
1489 102 88 32 32
MfeI C'AATT_G
Cuts at: 0 926 1743
Size: 926 817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 1709 1743
Size: 409 1300 34
Fragments arranged by size:
1300 409 34
MscI TGG'CCA
Cuts at: 0 1066 1183 1743
Size: 1066 117 560
Fragments arranged by size:
1066 560 117
MspA1I CmG'CkG
Cuts at: 0 355 649 1712 1743
Size: 355 294 1063 31
Fragments arranged by size:
1063 355 294 31
NaeI GCC'GGC
Cuts at: 0 1057 1743
Size: 1057 686
NcoI C'CATG_G
Cuts at: 0 1700 1743
Size: 1700 43
NgoMIV G'CCGG_C
Cuts at: 0 1055 1743
Size: 1055 688
NspI r_CATG'y
Cuts at: 0 471 1743
Size: 471 1272
PciI A'CATG_T
Cuts at: 0 467 1743
Size: 467 1276
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1424 1456 1743
Size: 1424 32 287
Fragments arranged by size:
1424 287 32
PpuMI rG'GwC_Cy
Cuts at: 0 1644 1743
Size: 1644 99
PsiI TTA'TAA
Cuts at: 0 1459 1743
Size: 1459 284
PspXI vC'TCGA_Gb
Cuts at: 0 119 1743
Size: 119 1624
PstI C_TGCA'G
Cuts at: 0 188 1743
Size: 188 1555
PvuII CAG'CTG
Cuts at: 0 355 649 1712 1743
Size: 355 294 1063 31
Fragments arranged by size:
1063 355 294 31
SapI GCTCTTCn'nnn_
Cuts at: 0 837 1030 1743
Size: 837 193 713
Fragments arranged by size:
837 713 193
ScaI AGT'ACT
Cuts at: 0 1487 1743
Size: 1487 256
SexAI A'CCwGG_T
Cuts at: 0 955 1089 1743
Size: 955 134 654
Fragments arranged by size:
955 654 134
SfcI C'TryA_G
Cuts at: 0 62 184 1743
Size: 62 122 1559
Fragments arranged by size:
1559 122 62
SmaI CCC'GGG
Cuts at: 0 856 1743
Size: 856 887
SmlI C'TyrA_G
Cuts at: 0 70 119 158 1743
Size: 70 49 39 1585
Fragments arranged by size:
1585 70 49 39
SrfI GCCC'GGGC
Cuts at: 0 856 1743
Size: 856 887
StyI C'CwwG_G
Cuts at: 0 461 1700 1733 1743
Size: 461 1239 33 10
Fragments arranged by size:
1239 461 33 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1100 1743
Size: 1100 643
TatI w'GTAC_w
Cuts at: 0 1349 1485 1743
Size: 1349 136 258
Fragments arranged by size:
1349 258 136
Tth111I GACn'n_nGTC
Cuts at: 0 1573 1743
Size: 1573 170
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1191 1698 1743
Size: 1191 507 45
Fragments arranged by size:
1191 507 45
XhoI C'TCGA_G
Cuts at: 0 70 119 1743
Size: 70 49 1624
Fragments arranged by size:
1624 70 49
XmaI C'CCGG_G
Cuts at: 0 854 1743
Size: 854 889
XmnI GAAnn'nnTTC
Cuts at: 0 1306 1487 1743
Size: 1306 181 256
Fragments arranged by size:
1306 256 181
ZraI GAC'GTC
Cuts at: 0 1000 1743
Size: 1000 743
Enzymes that do cut:
AatII AcuI AflIII AhdI AlwNI ApoI AvaI BamHI
BanI BanII BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI
BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I MfeI MmeI MscI MspA1I
NaeI NcoI NgoMIV NspI PciI PpiI PpuMI PsiI
PspXI PstI PvuII SapI ScaI SexAI SfcI SmaI
SmlI SrfI StyI TaqII TatI Tth111I XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BcgI BfrBI BmgBI BmtI BplI
BsaI BsaAI BsaWI BsgI BsiEI BsiWI BspEI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DrdI EagI EcoICRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MluI MslI NarI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PshAI PspOMI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SspI StuI SwaI TstI
XbaI