Restriction map for
pF1KB7837
(Linear) (Six-base) MAPSORT of: KIBB7837.seq Check: 9456 from: 1 to: 1620
KIBB7837.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 849 933 1353 1601 1620
Size: 429 420 84 420 248 19
Fragments arranged by size:
429 420 420 248 84 19
AflIII A'CryG_T
Cuts at: 0 421 1565 1620
Size: 421 1144 55
Fragments arranged by size:
1144 421 55
AleI CACnn'nnGTG
Cuts at: 0 992 1412 1620
Size: 992 420 208
Fragments arranged by size:
992 420 208
AlwNI CAG_nnn'CTG
Cuts at: 0 273 1620
Size: 273 1347
ApoI r'AATT_y
Cuts at: 0 245 815 983 1620
Size: 245 570 168 637
Fragments arranged by size:
637 570 245 168
BbsI GAAGACnn'nnnn_
Cuts at: 0 558 726 1146 1620
Size: 558 168 420 474
Fragments arranged by size:
558 474 420 168
BciVI GTATCCnnnnn_n'
Cuts at: 0 1135 1620
Size: 1135 485
BfrBI ATG'CAT
Cuts at: 0 1604 1620
Size: 1604 16
BglII A'GATC_T
Cuts at: 0 1036 1456 1620
Size: 1036 420 164
Fragments arranged by size:
1036 420 164
BmrI ACTGGGnnnn_n'
Cuts at: 0 617 1620
Size: 617 1003
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 316 514 1620
Size: 316 198 1106
Fragments arranged by size:
1106 316 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 509 677 761 845 929 1097 1181
Size: 311 198 168 84 84 84 168 84
Cuts at: 1181 1265 1349 1620
Size: 84 84 271
Fragments arranged by size:
311 271 198 168 168 84 84 84
84 84 84
BsaAI yAC'GTr
Cuts at: 0 424 1620
Size: 424 1196
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 1620
Size: 61 1559
BsmI GAATG_Cn'
Cuts at: 0 931 1114 1620
Size: 931 183 506
Fragments arranged by size:
931 506 183
BsrDI GCAATG_nn'
Cuts at: 0 856 1360 1512 1620
Size: 856 504 152 108
Fragments arranged by size:
856 504 152 108
BstBI TT'CG_AA
Cuts at: 0 465 633 717 1221 1305 1389 1620
Size: 465 168 84 504 84 84 231
Fragments arranged by size:
504 465 231 168 84 84 84
BstYI r'GATC_y
Cuts at: 0 63 1036 1456 1620
Size: 63 973 420 164
Fragments arranged by size:
973 420 164 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1452 1620
Size: 1452 168
BtsI GCAGTG_nn'
Cuts at: 0 940 1620
Size: 940 680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 918 953 1506 1541 1620
Size: 918 35 553 35 79
Fragments arranged by size:
918 553 79 35 35
DraI TTT'AAA
Cuts at: 0 840 1620
Size: 840 780
DraIII CAC_nnn'GTG
Cuts at: 0 571 1620
Size: 571 1049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 429 509 677 761 845 849 929
Size: 311 118 80 168 84 84 4 80
Cuts at: 929 933 1097 1181 1265 1349 1353 1601 1620
Size: 4 164 84 84 84 4 248 19
Fragments arranged by size:
311 248 168 164 118 84 84 84
84 84 80 80 19 4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 0 1168 1620
Size: 1168 452
EcoRI G'AATT_C
Cuts at: 0 815 983 1620
Size: 815 168 637
Fragments arranged by size:
815 637 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 545 577 1620
Size: 545 32 1043
Fragments arranged by size:
1043 545 32
HincII GTy'rAC
Cuts at: 0 1571 1620
Size: 1571 49
HindIII A'AGCT_T
Cuts at: 0 608 1280 1620
Size: 608 672 340
Fragments arranged by size:
672 608 340
HpaI GTT'AAC
Cuts at: 0 1571 1620
Size: 1571 49
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1620
Size: 415 1205
MslI CAynn'nnrTG
Cuts at: 0 588 992 1412 1620
Size: 588 404 420 208
Fragments arranged by size:
588 420 404 208
NdeI CA'TA_TG
Cuts at: 0 197 1620
Size: 197 1423
NsiI A_TGCA'T
Cuts at: 0 1606 1620
Size: 1606 14
NspI r_CATG'y
Cuts at: 0 1569 1579 1608 1620
Size: 1569 10 29 12
Fragments arranged by size:
1569 29 12 10
PciI A'CATG_T
Cuts at: 0 1565 1620
Size: 1565 55
PpuMI rG'GwC_Cy
Cuts at: 0 1168 1620
Size: 1168 452
PsiI TTA'TAA
Cuts at: 0 755 1175 1598 1620
Size: 755 420 423 22
Fragments arranged by size:
755 423 420 22
SfcI C'TryA_G
Cuts at: 0 84 125 1620
Size: 84 41 1495
Fragments arranged by size:
1495 84 41
StuI AGG'CCT
Cuts at: 0 946 1620
Size: 946 674
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 1620
Size: 55 1565
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 114 1620
Size: 114 1506
XmnI GAAnn'nnTTC
Cuts at: 0 442 815 946 983 1003 1366 1403 1620
Size: 442 373 131 37 20 363 37 217
Fragments arranged by size:
442 373 363 217 131 37 37 20
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApoI BbsI BciVI BfrBI
BglII BmrI BplI BpmI BsaAI BseRI BsmI BsrDI
BstBI BstYI BtgZI BtsI CspCI DraI DraIII Eco57MI
EcoO109I EcoRI Hin4I HincII HindIII HpaI MmeI MslI
NdeI NsiI NspI PciI PpuMI PsiI SfcI StuI
TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BclI BglI BlpI Bme1580I BmgBI BmtI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstZ17I Bsu36I BtgI ClaI DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StyI SwaI TatI TstI Tth111I XbaI XhoI XmaI
ZraI