Restriction map for
pF1KB7840
(Linear) (Six-base) MAPSORT of: KIBB7840.seq Check: 3553 from: 1 to: 1845
KIBB7840.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AatII G_ACGT'C
Cuts at: 0 1587 1845
Size: 1587 258
AccI GT'mk_AC
Cuts at: 0 710 1845
Size: 710 1135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1466 1845
Size: 1466 379
AfeI AGC'GCT
Cuts at: 0 865 1845
Size: 865 980
AflII C'TTAA_G
Cuts at: 0 298 1845
Size: 298 1547
AflIII A'CryG_T
Cuts at: 0 1416 1845
Size: 1416 429
AleI CACnn'nnGTG
Cuts at: 0 419 1845
Size: 419 1426
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 450 1013 1723 1747 1845
Size: 450 563 710 24 98
Fragments arranged by size:
710 563 450 98 24
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1310 1342 1845
Size: 1310 32 503
Fragments arranged by size:
1310 503 32
AlwNI CAG_nnn'CTG
Cuts at: 0 396 669 1264 1845
Size: 396 273 595 581
Fragments arranged by size:
595 581 396 273
ApaI G_GGCC'C
Cuts at: 0 1041 1521 1845
Size: 1041 480 324
Fragments arranged by size:
1041 480 324
ApaLI G'TGCA_C
Cuts at: 0 172 786 1472 1845
Size: 172 614 686 373
Fragments arranged by size:
686 614 373 172
AvaI C'yCGr_G
Cuts at: 0 1088 1339 1820 1845
Size: 1088 251 481 25
Fragments arranged by size:
1088 481 251 25
AvrII C'CTAG_G
Cuts at: 0 1689 1845
Size: 1689 156
BanI G'GyrC_C
Cuts at: 0 23 180 285 622 632 1231 1845
Size: 23 157 105 337 10 599 614
Fragments arranged by size:
614 599 337 157 105 23 10
BanII G_rGCy'C
Cuts at: 0 470 1041 1115 1274 1296 1328 1521 1845
Size: 470 571 74 159 22 32 193 324
Fragments arranged by size:
571 470 324 193 159 74 32 22
BbeI G_GCGC'C
Cuts at: 0 27 1845
Size: 27 1818
BbvCI CC'TCA_GC
Cuts at: 0 797 1845
Size: 797 1048
BglI GCCn_nnn'nGGC
Cuts at: 0 614 1845
Size: 614 1231
BglII A'GATC_T
Cuts at: 0 131 1845
Size: 131 1714
Bme1580I G_kGCm'C
Cuts at: 0 103 176 290 790 1041 1476 1521 1845
Size: 103 73 114 500 251 435 45 324
Fragments arranged by size:
500 435 324 251 114 103 73 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 554 1330 1845
Size: 554 776 515
Fragments arranged by size:
776 554 515
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1290 1310 1845
Size: 1290 20 535
Fragments arranged by size:
1290 535 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 144 1106 1164 1620 1632 1845
Size: 29 115 962 58 456 12 213
Fragments arranged by size:
962 456 213 115 58 29 12
Bpu10I CC'TnA_GC
Cuts at: 0 797 1739 1845
Size: 797 942 106
Fragments arranged by size:
942 797 106
BsaI GGTCTCn'nnnn_
Cuts at: 0 1210 1750 1845
Size: 1210 540 95
Fragments arranged by size:
1210 540 95
BsaAI yAC'GTr
Cuts at: 0 659 1845
Size: 659 1186
BsaHI Gr'CG_yC
Cuts at: 0 24 720 1584 1845
Size: 24 696 864 261
Fragments arranged by size:
864 696 261 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 226 256 1291 1310 1321 1340 1845
Size: 226 30 1035 19 11 19 505
Fragments arranged by size:
1035 505 226 30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 175 286 466 574 1282 1845
Size: 175 111 180 108 708 563
Fragments arranged by size:
708 563 180 175 111 108
BseYI C'CCAG_C
Cuts at: 0 1044 1484 1522 1845
Size: 1044 440 38 323
Fragments arranged by size:
1044 440 323 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 420 632 1743 1845
Size: 382 38 212 1111 102
Fragments arranged by size:
1111 382 212 102 38
BsiEI CG_ry'CG
Cuts at: 0 1401 1845
Size: 1401 444
BsiHKAI G_wGCw'C
Cuts at: 0 176 470 790 950 1411 1476 1845
Size: 176 294 320 160 461 65 369
Fragments arranged by size:
461 369 320 294 176 160 65
BsmI GAATG_Cn'
Cuts at: 0 739 1845
Size: 739 1106
Bsp1286I G_dGCh'C
Cuts at: 0 103 176 290 470 790 950 1041 1115
Size: 103 73 114 180 320 160 91 74
Cuts at: 1115 1274 1296 1328 1411 1476 1521 1845
Size: 159 22 32 83 65 45 324
Fragments arranged by size:
324 320 180 160 159 114 103 91
83 74 73 65 45 32 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 300 1382 1845
Size: 300 1082 463
Fragments arranged by size:
1082 463 300
BsrBI CCG'CTC
Cuts at: 0 29 1845
Size: 29 1816
BsrDI GCAATG_nn'
Cuts at: 0 141 1757 1845
Size: 141 1616 88
Fragments arranged by size:
1616 141 88
BsrFI r'CCGG_y
Cuts at: 0 1150 1845
Size: 1150 695
BstAPI GCAn_nnn'nTGC
Cuts at: 0 171 180 396 405 601 1845
Size: 171 9 216 9 196 1244
Fragments arranged by size:
1244 216 196 171 9 9
BstEII G'GTnAC_C
Cuts at: 0 534 1845
Size: 534 1311
BstYI r'GATC_y
Cuts at: 0 131 1845
Size: 131 1714
Bsu36I CC'TnA_GG
Cuts at: 0 1265 1845
Size: 1265 580
BtgI C'CryG_G
Cuts at: 0 117 154 1845
Size: 117 37 1691
Fragments arranged by size:
1691 117 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 576 1845
Size: 576 1269
BtsI GCAGTG_nn'
Cuts at: 0 103 176 602 891 1338 1745 1845
Size: 103 73 426 289 447 407 100
Fragments arranged by size:
447 426 407 289 103 100 73
DraIII CAC_nnn'GTG
Cuts at: 0 180 1845
Size: 180 1665
EaeI y'GGCC_r
Cuts at: 0 1362 1845
Size: 1362 483
EarI CTCTTCn'nnn_
Cuts at: 0 443 1845
Size: 443 1402
EcoICRI GAG'CTC
Cuts at: 0 468 1845
Size: 468 1377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 144 1106 1164 1466 1620 1632 1845
Size: 29 115 962 58 302 154 12 213
Fragments arranged by size:
962 302 213 154 115 58 29 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 337 1845
Size: 337 1508
EcoO109I rG'GnC_Cy
Cuts at: 0 14 57 348 1038 1261 1517 1518 1845
Size: 14 43 291 690 223 256 1 327
Fragments arranged by size:
690 327 291 256 223 43 14 1
FspI TGC'GCA
Cuts at: 0 523 1845
Size: 523 1322
FspAI rTGC'GCAy
Cuts at: 0 523 1845
Size: 523 1322
HaeII r_GCGC'y
Cuts at: 0 27 234 867 1252 1491 1845
Size: 27 207 633 385 239 354
Fragments arranged by size:
633 385 354 239 207 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 1061 1093 1594 1612 1626 1644
Size: 238 32 791 32 501 18 14 18
Cuts at: 1644 1685 1717 1797 1829 1845
Size: 41 32 80 32 16
Fragments arranged by size:
791 501 238 80 41 32 32 32
32 18 18 16 14
HincII GTy'rAC
Cuts at: 0 360 678 1845
Size: 360 318 1167
Fragments arranged by size:
1167 360 318
HindIII A'AGCT_T
Cuts at: 0 845 1845
Size: 845 1000
KasI G'GCGC_C
Cuts at: 0 23 1845
Size: 23 1822
MluI A'CGCG_T
Cuts at: 0 1416 1845
Size: 1416 429
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 1845
Size: 269 1576
MslI CAynn'nnrTG
Cuts at: 0 419 595 995 1750 1845
Size: 419 176 400 755 95
Fragments arranged by size:
755 419 400 176 95
MspA1I CmG'CkG
Cuts at: 0 156 329 393 405 489 1010 1142 1845
Size: 156 173 64 12 84 521 132 703
Fragments arranged by size:
703 521 173 156 132 84 64 12
NaeI GCC'GGC
Cuts at: 0 1152 1845
Size: 1152 693
NarI GG'CG_CC
Cuts at: 0 24 1845
Size: 24 1821
NgoMIV G'CCGG_C
Cuts at: 0 1150 1845
Size: 1150 695
NspI r_CATG'y
Cuts at: 0 169 1000 1845
Size: 169 831 845
Fragments arranged by size:
845 831 169
PasI CC'CwG_GG
Cuts at: 0 1514 1787 1845
Size: 1514 273 58
Fragments arranged by size:
1514 273 58
PfoI T'CCnGG_A
Cuts at: 0 16 1845
Size: 16 1829
PmlI CAC'GTG
Cuts at: 0 659 1845
Size: 659 1186
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1310 1342 1845
Size: 1310 32 503
Fragments arranged by size:
1310 503 32
PpuMI rG'GwC_Cy
Cuts at: 0 14 57 348 1845
Size: 14 43 291 1497
Fragments arranged by size:
1497 291 43 14
PspOMI G'GGCC_C
Cuts at: 0 1037 1517 1845
Size: 1037 480 328
Fragments arranged by size:
1037 480 328
PstI C_TGCA'G
Cuts at: 0 1099 1845
Size: 1099 746
PvuII CAG'CTG
Cuts at: 0 329 393 405 1142 1845
Size: 329 64 12 737 703
Fragments arranged by size:
737 703 329 64 12
SacI G_AGCT'C
Cuts at: 0 470 1845
Size: 470 1375
SacII CC_GC'GG
Cuts at: 0 157 1845
Size: 157 1688
SanDI GG'GwC_CC
Cuts at: 0 14 1845
Size: 14 1831
ScaI AGT'ACT
Cuts at: 0 433 928 1845
Size: 433 495 917
Fragments arranged by size:
917 495 433
SfcI C'TryA_G
Cuts at: 0 87 1020 1095 1256 1845
Size: 87 933 75 161 589
Fragments arranged by size:
933 589 161 87 75
SfoI GGC'GCC
Cuts at: 0 25 1845
Size: 25 1820
SmlI C'TyrA_G
Cuts at: 0 298 1088 1845
Size: 298 790 757
Fragments arranged by size:
790 757 298
SphI G_CATG'C
Cuts at: 0 169 1000 1845
Size: 169 831 845
Fragments arranged by size:
845 831 169
StuI AGG'CCT
Cuts at: 0 296 617 1073 1845
Size: 296 321 456 772
Fragments arranged by size:
772 456 321 296
StyI C'CwwG_G
Cuts at: 0 963 1101 1689 1845
Size: 963 138 588 156
Fragments arranged by size:
963 588 156 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 273 610 650 1387 1527 1589 1845
Size: 273 337 40 737 140 62 256
Fragments arranged by size:
737 337 273 256 140 62 40
TatI w'GTAC_w
Cuts at: 0 431 926 1845
Size: 431 495 919
Fragments arranged by size:
919 495 431
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 226 258 1291 1323 1455 1487 1845
Size: 226 32 1033 32 132 32 358
Fragments arranged by size:
1033 358 226 132 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 346 1845
Size: 346 1499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1317 1845
Size: 1317 528
XhoI C'TCGA_G
Cuts at: 0 1088 1845
Size: 1088 757
ZraI GAC'GTC
Cuts at: 0 1585 1845
Size: 1585 260
Enzymes that do cut:
AatII AccI AcuI AfeI AflII AflIII AleI AlfI
AloI AlwNI ApaI ApaLI AvaI AvrII BanI BanII
BbeI BbvCI BglI BglII Bme1580I BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI
HaeII Hin4I HincII HindIII KasI MluI MmeI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PfoI PmlI
PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI
ScaI SfcI SfoI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AgeI AhdI ApoI AscI AseI
AsiSI BaeI BamHI BbsI BcgI BciVI BclI BfrBI
BlpI BmgBI BmtI BpuEI BsaBI BsaWI BsiWI BsmBI
BspEI BspHI BsrGI BssHII BssSI BstBI BstXI BstZ17I
ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV
FalI FseI HpaI KpnI MfeI MscI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI XbaI XmaI XmnI