Restriction map for
pF1KB7842
(Linear) (Six-base) MAPSORT of: KIBB7842.seq Check: 3105 from: 1 to: 1851
KIBB7842.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 171 874 1191 1851
Size: 71 100 703 317 660
Fragments arranged by size:
703 660 317 100 71
AflIII A'CryG_T
Cuts at: 0 595 1024 1851
Size: 595 429 827
Fragments arranged by size:
827 595 429
AleI CACnn'nnGTG
Cuts at: 0 1418 1851
Size: 1418 433
ApoI r'AATT_y
Cuts at: 0 43 294 700 1299 1636 1801 1851
Size: 43 251 406 599 337 165 50
Fragments arranged by size:
599 406 337 251 165 50 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1617 1650 1851
Size: 1617 33 201
Fragments arranged by size:
1617 201 33
BanII G_rGCy'C
Cuts at: 0 715 1851
Size: 715 1136
BbsI GAAGACnn'nnnn_
Cuts at: 0 105 277 1207 1851
Size: 105 172 930 644
Fragments arranged by size:
930 644 172 105
BglII A'GATC_T
Cuts at: 0 1790 1851
Size: 1790 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 923 1851
Size: 923 928
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1562 1812 1851
Size: 1562 250 39
Fragments arranged by size:
1562 250 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 1607 1796 1851
Size: 767 840 189 55
Fragments arranged by size:
840 767 189 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1414 1851
Size: 1414 437
BsaI GGTCTCn'nnnn_
Cuts at: 0 410 1419 1851
Size: 410 1009 432
Fragments arranged by size:
1009 432 410
BsaAI yAC'GTr
Cuts at: 0 492 1851
Size: 492 1359
BsaBI GATnn'nnATC
Cuts at: 0 560 1851
Size: 560 1291
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 139 1372 1851
Size: 136 3 1233 479
Fragments arranged by size:
1233 479 136 3
BseYI C'CCAG_C
Cuts at: 0 129 630 1463 1715 1851
Size: 129 501 833 252 136
Fragments arranged by size:
833 501 252 136 129
BsiHKAI G_wGCw'C
Cuts at: 0 715 1851
Size: 715 1136
BsmI GAATG_Cn'
Cuts at: 0 981 1851
Size: 981 870
Bsp1286I G_dGCh'C
Cuts at: 0 715 1851
Size: 715 1136
BspHI T'CATG_A
Cuts at: 0 876 1851
Size: 876 975
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 203 1851
Size: 203 1648
BsrDI GCAATG_nn'
Cuts at: 0 778 813 1114 1851
Size: 778 35 301 737
Fragments arranged by size:
778 737 301 35
BstYI r'GATC_y
Cuts at: 0 308 1324 1389 1790 1851
Size: 308 1016 65 401 61
Fragments arranged by size:
1016 401 308 65 61
BtgI C'CryG_G
Cuts at: 0 986 1070 1851
Size: 986 84 781
Fragments arranged by size:
986 781 84
BtsI GCAGTG_nn'
Cuts at: 0 92 1618 1851
Size: 92 1526 233
Fragments arranged by size:
1526 233 92
EarI CTCTTCn'nnn_
Cuts at: 0 347 1079 1520 1781 1851
Size: 347 732 441 261 70
Fragments arranged by size:
732 441 347 261 70
EcoICRI GAG'CTC
Cuts at: 0 713 1851
Size: 713 1138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 171 767 874 1191 1607 1796 1851
Size: 71 100 596 107 317 416 189 55
Fragments arranged by size:
596 416 317 189 107 100 71 55
EcoRI G'AATT_C
Cuts at: 0 294 1801 1851
Size: 294 1507 50
Fragments arranged by size:
1507 294 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 293 325 702 734 1374 1406 1851
Size: 293 32 377 32 640 32 445
Fragments arranged by size:
640 445 377 293 32 32 32
HindIII A'AGCT_T
Cuts at: 0 615 1851
Size: 615 1236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1367 1851
Size: 1367 484
MslI CAynn'nnrTG
Cuts at: 0 596 930 1014 1098 1418 1851
Size: 596 334 84 84 320 433
Fragments arranged by size:
596 433 334 320 84 84
NcoI C'CATG_G
Cuts at: 0 986 1070 1851
Size: 986 84 781
Fragments arranged by size:
986 781 84
NdeI CA'TA_TG
Cuts at: 0 659 1851
Size: 659 1192
NspI r_CATG'y
Cuts at: 0 599 1028 1580 1653 1851
Size: 599 429 552 73 198
Fragments arranged by size:
599 552 429 198 73
PciI A'CATG_T
Cuts at: 0 595 1024 1851
Size: 595 429 827
Fragments arranged by size:
827 595 429
PmlI CAC'GTG
Cuts at: 0 492 1851
Size: 492 1359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 1851
Size: 184 32 1635
Fragments arranged by size:
1635 184 32
PsiI TTA'TAA
Cuts at: 0 845 1851
Size: 845 1006
SacI G_AGCT'C
Cuts at: 0 715 1851
Size: 715 1136
SexAI A'CCwGG_T
Cuts at: 0 400 1851
Size: 400 1451
SmlI C'TyrA_G
Cuts at: 0 1393 1851
Size: 1393 458
StyI C'CwwG_G
Cuts at: 0 986 1070 1851
Size: 986 84 781
Fragments arranged by size:
986 781 84
TatI w'GTAC_w
Cuts at: 0 1552 1851
Size: 1552 299
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 353 1851
Size: 353 1498
XmnI GAAnn'nnTTC
Cuts at: 0 23 757 784 868 925 1204 1851
Size: 23 734 27 84 57 279 647
Fragments arranged by size:
734 647 279 84 57 27 23
Enzymes that do cut:
AcuI AflIII AleI ApoI BaeI BanII BbsI BglII
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BseRI
BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BstYI
BtgI BtsI EarI EcoICRI Eco57MI EcoRI Hin4I HindIII
MmeI MslI NcoI NdeI NspI PciI PmlI PpiI
PsiI SacI SexAI SmlI StyI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI
Bpu10I BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoNI EcoO109I EcoRV
FalI FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MscI MspA1I NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XhoI XmaI ZraI