Restriction map for
pF1KB7851
(Linear) (Six-base) MAPSORT of: KIBB7851.seq Check: 9924 from: 1 to: 1644
KIBB7851.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1389 1644
Size: 1389 255
AccI GT'mk_AC
Cuts at: 0 962 1644
Size: 962 682
Acc65I G'GTAC_C
Cuts at: 0 935 1644
Size: 935 709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 1644
Size: 897 747
AgeI A'CCGG_T
Cuts at: 0 1074 1644
Size: 1074 570
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1443 1644
Size: 1443 201
AlwNI CAG_nnn'CTG
Cuts at: 0 563 1644
Size: 563 1081
ApaI G_GGCC'C
Cuts at: 0 252 659 881 1498 1571 1644
Size: 252 407 222 617 73 73
Fragments arranged by size:
617 407 252 222 73 73
ApoI r'AATT_y
Cuts at: 0 160 1644
Size: 160 1484
AvaI C'yCGr_G
Cuts at: 0 713 1063 1414 1644
Size: 713 350 351 230
Fragments arranged by size:
713 351 350 230
BamHI G'GATC_C
Cuts at: 0 606 769 1644
Size: 606 163 875
Fragments arranged by size:
875 606 163
BanI G'GyrC_C
Cuts at: 0 643 935 1014 1059 1410 1644
Size: 643 292 79 45 351 234
Fragments arranged by size:
643 351 292 234 79 45
BanII G_rGCy'C
Cuts at: 0 252 659 853 881 1286 1403 1498 1571
Size: 252 407 194 28 405 117 95 73
Cuts at: 1571 1594 1644
Size: 23 50
Fragments arranged by size:
407 405 252 194 117 95 73 50
28 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 684 1352 1475 1644
Size: 10 674 668 123 169
Fragments arranged by size:
674 668 169 123 10
BbvCI CC'TCA_GC
Cuts at: 0 733 785 976 1229 1644
Size: 733 52 191 253 415
Fragments arranged by size:
733 415 253 191 52
BglI GCCn_nnn'nGGC
Cuts at: 0 542 1644
Size: 542 1102
BlpI GC'TnA_GC
Cuts at: 0 241 847 1644
Size: 241 606 797
Fragments arranged by size:
797 606 241
Bme1580I G_kGCm'C
Cuts at: 0 252 386 396 659 881 1019 1045 1377
Size: 252 134 10 263 222 138 26 332
Cuts at: 1377 1498 1571 1644
Size: 121 73 73
Fragments arranged by size:
332 263 252 222 138 134 121 73
73 26 10
BmgBI CAC'GTC
Cuts at: 0 578 1644
Size: 578 1066
BmrI ACTGGGnnnn_n'
Cuts at: 0 857 1419 1644
Size: 857 562 225
Fragments arranged by size:
857 562 225
BmtI G_CTAG'C
Cuts at: 0 1249 1644
Size: 1249 395
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1305 1644
Size: 1305 339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 607 1120 1482 1585 1644
Size: 121 486 513 362 103 59
Fragments arranged by size:
513 486 362 121 103 59
Bpu10I CC'TnA_GC
Cuts at: 0 733 785 888 976 1229 1391 1397 1644
Size: 733 52 103 88 253 162 6 247
Fragments arranged by size:
733 253 247 162 103 88 52 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 1423 1644
Size: 54 1369 221
Fragments arranged by size:
1369 221 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 1060 1644
Size: 1060 584
BsaHI Gr'CG_yC
Cuts at: 0 131 811 1155 1366 1644
Size: 131 680 344 211 278
Fragments arranged by size:
680 344 278 211 131
BsaWI w'CCGG_w
Cuts at: 0 633 1074 1644
Size: 633 441 570
Fragments arranged by size:
633 570 441
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1624 1644
Size: 1624 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 1074 1321 1644
Size: 133 941 247 323
Fragments arranged by size:
941 323 247 133
BseYI C'CCAG_C
Cuts at: 0 370 597 880 1019 1025 1171 1501 1561
Size: 370 227 283 139 6 146 330 60
Cuts at: 1561 1616 1644
Size: 55 28
Fragments arranged by size:
370 330 283 227 146 139 60 55
28 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 398 979 1235 1367 1644
Size: 265 133 581 256 132 277
Fragments arranged by size:
581 277 265 256 133 132
BsiHKAI G_wGCw'C
Cuts at: 0 1594 1634 1644
Size: 1594 40 10
Fragments arranged by size:
1594 40 10
BsmI GAATG_Cn'
Cuts at: 0 275 1644
Size: 275 1369
Bsp1286I G_dGCh'C
Cuts at: 0 252 386 396 659 853 881 1019 1045
Size: 252 134 10 263 194 28 138 26
Cuts at: 1045 1286 1377 1403 1498 1571 1594 1634 1644
Size: 241 91 26 95 73 23 40 10
Fragments arranged by size:
263 252 241 194 138 134 95 91
73 40 28 26 26 23 10 10
BspEI T'CCGG_A
Cuts at: 0 633 1644
Size: 633 1011
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 947 1389 1644
Size: 947 442 255
Fragments arranged by size:
947 442 255
BsrBI CCG'CTC
Cuts at: 0 479 1205 1644
Size: 479 726 439
Fragments arranged by size:
726 479 439
BsrDI GCAATG_nn'
Cuts at: 0 134 1644
Size: 134 1510
BsrFI r'CCGG_y
Cuts at: 0 760 1074 1157 1189 1644
Size: 760 314 83 32 455
Fragments arranged by size:
760 455 314 83 32
BstXI CCAn_nnnn'nTGG
Cuts at: 0 685 822 1644
Size: 685 137 822
Fragments arranged by size:
822 685 137
BstYI r'GATC_y
Cuts at: 0 77 606 636 769 1644
Size: 77 529 30 133 875
Fragments arranged by size:
875 529 133 77 30
Bsu36I CC'TnA_GG
Cuts at: 0 920 1644
Size: 920 724
BtgI C'CryG_G
Cuts at: 0 1055 1610 1644
Size: 1055 555 34
Fragments arranged by size:
1055 555 34
BtsI GCAGTG_nn'
Cuts at: 0 386 1218 1644
Size: 386 832 426
Fragments arranged by size:
832 426 386
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1188 1223 1644
Size: 1188 35 421
Fragments arranged by size:
1188 421 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 688 1644
Size: 688 956
EaeI y'GGCC_r
Cuts at: 0 758 1644
Size: 758 886
EarI CTCTTCn'nnn_
Cuts at: 0 501 520 1107 1644
Size: 501 19 587 537
Fragments arranged by size:
587 537 501 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 1644
Size: 28 1616
EcoICRI GAG'CTC
Cuts at: 0 1592 1644
Size: 1592 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 607 897 1120 1482 1585 1644
Size: 121 486 290 223 362 103 59
Fragments arranged by size:
486 362 290 223 121 103 59
EcoO109I rG'GnC_Cy
Cuts at: 0 249 471 655 656 877 1494 1495 1549
Size: 249 222 184 1 221 617 1 54
Cuts at: 1549 1567 1568 1644
Size: 18 1 76
Fragments arranged by size:
617 249 222 221 184 76 54 18
1 1 1
EcoRI G'AATT_C
Cuts at: 0 160 1644
Size: 160 1484
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1464 1644
Size: 1464 180
HaeII r_GCGC'y
Cuts at: 0 1032 1246 1461 1644
Size: 1032 214 215 183
Fragments arranged by size:
1032 215 214 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 442 463 474 495 539 562 571 594
Size: 442 21 11 21 44 23 9 23
Cuts at: 594 1644
Size: 1050
Fragments arranged by size:
1050 442 44 23 23 21 21 11
9
KpnI G_GTAC'C
Cuts at: 0 939 1644
Size: 939 705
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1283 1644
Size: 1283 361
MslI CAynn'nnrTG
Cuts at: 0 358 1425 1644
Size: 358 1067 219
Fragments arranged by size:
1067 358 219
MspA1I CmG'CkG
Cuts at: 0 84 597 663 884 1047 1164 1171 1501
Size: 84 513 66 221 163 117 7 330
Cuts at: 1501 1644
Size: 143
Fragments arranged by size:
513 330 221 163 143 117 84 66
7
NaeI GCC'GGC
Cuts at: 0 1159 1644
Size: 1159 485
NcoI C'CATG_G
Cuts at: 0 1055 1644
Size: 1055 589
NgoMIV G'CCGG_C
Cuts at: 0 1157 1644
Size: 1157 487
NheI G'CTAG_C
Cuts at: 0 1245 1644
Size: 1245 399
NspI r_CATG'y
Cuts at: 0 1434 1609 1644
Size: 1434 175 35
Fragments arranged by size:
1434 175 35
PasI CC'CwG_GG
Cuts at: 0 1003 1644
Size: 1003 641
PfoI T'CCnGG_A
Cuts at: 0 32 98 765 1644
Size: 32 66 667 879
Fragments arranged by size:
879 667 66 32
PpuMI rG'GwC_Cy
Cuts at: 0 471 1644
Size: 471 1173
PspOMI G'GGCC_C
Cuts at: 0 248 655 877 1494 1567 1644
Size: 248 407 222 617 73 77
Fragments arranged by size:
617 407 248 222 77 73
PstI C_TGCA'G
Cuts at: 0 944 1644
Size: 944 700
PvuII CAG'CTG
Cuts at: 0 84 1171 1644
Size: 84 1087 473
Fragments arranged by size:
1087 473 84
SacI G_AGCT'C
Cuts at: 0 1594 1644
Size: 1594 50
SapI GCTCTTCn'nnn_
Cuts at: 0 501 1644
Size: 501 1143
SbfI CC_TGCA'GG
Cuts at: 0 944 1644
Size: 944 700
SfcI C'TryA_G
Cuts at: 0 564 940 1644
Size: 564 376 704
Fragments arranged by size:
704 564 376
SmaI CCC'GGG
Cuts at: 0 1416 1644
Size: 1416 228
SmlI C'TyrA_G
Cuts at: 0 69 1438 1644
Size: 69 1369 206
Fragments arranged by size:
1369 206 69
SphI G_CATG'C
Cuts at: 0 1434 1609 1644
Size: 1434 175 35
Fragments arranged by size:
1434 175 35
StyI C'CwwG_G
Cuts at: 0 290 1055 1579 1644
Size: 290 765 524 65
Fragments arranged by size:
765 524 290 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 832 967 1644
Size: 832 135 677
Fragments arranged by size:
832 677 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1624 1644
Size: 1624 20
XmaI C'CCGG_G
Cuts at: 0 1414 1644
Size: 1414 230
XmnI GAAnn'nnTTC
Cuts at: 0 301 1644
Size: 301 1343
Enzymes that do cut:
AarI AccI Acc65I AcuI AgeI AlfI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I KpnI MmeI MslI MspA1I NaeI NcoI NgoMIV
NheI NspI PasI PfoI PpuMI PspOMI PstI PvuII
SacI SapI SbfI SfcI SmaI SmlI SphI StyI
TaqII TstI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AhdI AleI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BciVI BclI BfrBI BglII BsaAI BsaBI BsiEI BsiWI
BsmBI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI ClaI DraI DraIII EagI EcoNI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI MfeI
MluI MscI NarI NdeI NotI NruI NsiI PacI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI TatI Tth111I XbaI XcmI XhoI ZraI