Restriction map for
pF1KB7854
(Linear) (Six-base) MAPSORT of: KIBB7854.seq Check: 4757 from: 1 to: 1875
KIBB7854.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 677 894 1875
Size: 500 177 217 981
Fragments arranged by size:
981 500 217 177
AflIII A'CryG_T
Cuts at: 0 713 1875
Size: 713 1162
AleI CACnn'nnGTG
Cuts at: 0 469 1875
Size: 469 1406
ApaI G_GGCC'C
Cuts at: 0 796 1875
Size: 796 1079
ApaLI G'TGCA_C
Cuts at: 0 514 1875
Size: 514 1361
ApoI r'AATT_y
Cuts at: 0 396 679 1875
Size: 396 283 1196
Fragments arranged by size:
1196 396 283
AseI AT'TA_AT
Cuts at: 0 161 1875
Size: 161 1714
AvaI C'yCGr_G
Cuts at: 0 221 430 524 1875
Size: 221 209 94 1351
Fragments arranged by size:
1351 221 209 94
BanI G'GyrC_C
Cuts at: 0 295 1875
Size: 295 1580
BanII G_rGCy'C
Cuts at: 0 439 796 1875
Size: 439 357 1079
Fragments arranged by size:
1079 439 357
BbsI GAAGACnn'nnnn_
Cuts at: 0 275 900 923 1236 1875
Size: 275 625 23 313 639
Fragments arranged by size:
639 625 313 275 23
BbvCI CC'TCA_GC
Cuts at: 0 704 1875
Size: 704 1171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 421 455 1875
Size: 421 34 1420
Fragments arranged by size:
1420 421 34
BclI T'GATC_A
Cuts at: 0 720 1875
Size: 720 1155
BfrBI ATG'CAT
Cuts at: 0 1654 1875
Size: 1654 221
BglI GCCn_nnn'nGGC
Cuts at: 0 145 1875
Size: 145 1730
Bme1580I G_kGCm'C
Cuts at: 0 47 518 584 796 1875
Size: 47 471 66 212 1079
Fragments arranged by size:
1079 471 212 66 47
BmgBI CAC'GTC
Cuts at: 0 579 1080 1875
Size: 579 501 795
Fragments arranged by size:
795 579 501
BmrI ACTGGGnnnn_n'
Cuts at: 0 47 1875
Size: 47 1828
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1818 1875
Size: 1818 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1875
Size: 412 1463
Bpu10I CC'TnA_GC
Cuts at: 0 704 1875
Size: 704 1171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1140 1875
Size: 1140 735
BsaI GGTCTCn'nnnn_
Cuts at: 0 838 1875
Size: 838 1037
BsaAI yAC'GTr
Cuts at: 0 1623 1875
Size: 1623 252
BsaBI GATnn'nnATC
Cuts at: 0 1102 1875
Size: 1102 773
BsaWI w'CCGG_w
Cuts at: 0 633 847 1477 1875
Size: 633 214 630 398
Fragments arranged by size:
633 630 398 214
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 700 730 1875
Size: 700 30 1145
Fragments arranged by size:
1145 700 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 341 721 1125 1335 1573 1875
Size: 341 380 404 210 238 302
Fragments arranged by size:
404 380 341 302 238 210
BsiHKAI G_wGCw'C
Cuts at: 0 439 518 1126 1616 1799 1875
Size: 439 79 608 490 183 76
Fragments arranged by size:
608 490 439 183 79 76
Bsp1286I G_dGCh'C
Cuts at: 0 47 439 518 584 796 1126 1616 1799
Size: 47 392 79 66 212 330 490 183
Cuts at: 1799 1875
Size: 76
Fragments arranged by size:
490 392 330 212 183 79 76 66
47
BspEI T'CCGG_A
Cuts at: 0 633 1875
Size: 633 1242
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1475 1875
Size: 1475 400
BsrBI CCG'CTC
Cuts at: 0 1287 1875
Size: 1287 588
BsrDI GCAATG_nn'
Cuts at: 0 998 1875
Size: 998 877
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1057 1875
Size: 1057 818
BtgI C'CryG_G
Cuts at: 0 1550 1721 1875
Size: 1550 171 154
Fragments arranged by size:
1550 171 154
BtsI GCAGTG_nn'
Cuts at: 0 1527 1875
Size: 1527 348
EaeI y'GGCC_r
Cuts at: 0 590 1875
Size: 590 1285
EarI CTCTTCn'nnn_
Cuts at: 0 72 533 1037 1082 1277 1378 1444 1550
Size: 72 461 504 45 195 101 66 106
Cuts at: 1550 1875
Size: 325
Fragments arranged by size:
504 461 325 195 106 101 72 66
45
EcoICRI GAG'CTC
Cuts at: 0 437 1875
Size: 437 1438
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 500 677 894 1875
Size: 412 88 177 217 981
Fragments arranged by size:
981 412 217 177 88
EcoO109I rG'GnC_Cy
Cuts at: 0 792 793 990 1683 1875
Size: 792 1 197 693 192
Fragments arranged by size:
792 693 197 192 1
EcoRI G'AATT_C
Cuts at: 0 396 1875
Size: 396 1479
EcoRV GAT'ATC
Cuts at: 0 1700 1875
Size: 1700 175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 132 1035 1875
Size: 132 903 840
Fragments arranged by size:
903 840 132
HaeII r_GCGC'y
Cuts at: 0 605 1875
Size: 605 1270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 329 361 539 568 571 600 712 744
Size: 329 32 178 29 3 29 112 32
Cuts at: 744 860 892 1120 1152 1408 1440 1875
Size: 116 32 228 32 256 32 435
Fragments arranged by size:
435 329 256 228 178 116 112 32
32 32 32 32 29 29 3
HindIII A'AGCT_T
Cuts at: 0 1738 1875
Size: 1738 137
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 1875
Size: 891 984
MscI TGG'CCA
Cuts at: 0 592 1875
Size: 592 1283
MslI CAynn'nnrTG
Cuts at: 0 451 469 1108 1768 1864 1875
Size: 451 18 639 660 96 11
Fragments arranged by size:
660 639 451 96 18 11
NcoI C'CATG_G
Cuts at: 0 1550 1875
Size: 1550 325
NsiI A_TGCA'T
Cuts at: 0 1656 1875
Size: 1656 219
NspI r_CATG'y
Cuts at: 0 717 1875
Size: 717 1158
PciI A'CATG_T
Cuts at: 0 713 1875
Size: 713 1162
PpuMI rG'GwC_Cy
Cuts at: 0 990 1875
Size: 990 885
PsiI TTA'TAA
Cuts at: 0 1193 1875
Size: 1193 682
PspOMI G'GGCC_C
Cuts at: 0 792 1875
Size: 792 1083
PstI C_TGCA'G
Cuts at: 0 1472 1875
Size: 1472 403
SacI G_AGCT'C
Cuts at: 0 439 1875
Size: 439 1436
SapI GCTCTTCn'nnn_
Cuts at: 0 72 533 1277 1875
Size: 72 461 744 598
Fragments arranged by size:
744 598 461 72
SfcI C'TryA_G
Cuts at: 0 1468 1875
Size: 1468 407
SmaI CCC'GGG
Cuts at: 0 526 1875
Size: 526 1349
SmlI C'TyrA_G
Cuts at: 0 221 1119 1875
Size: 221 898 756
Fragments arranged by size:
898 756 221
SpeI A'CTAG_T
Cuts at: 0 30 1875
Size: 30 1845
StuI AGG'CCT
Cuts at: 0 1321 1875
Size: 1321 554
StyI C'CwwG_G
Cuts at: 0 143 291 1550 1875
Size: 143 148 1259 325
Fragments arranged by size:
1259 325 148 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 610 1875
Size: 610 1265
TatI w'GTAC_w
Cuts at: 0 413 710 746 1875
Size: 413 297 36 1129
Fragments arranged by size:
1129 413 297 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 452 484 1875
Size: 452 32 1391
Fragments arranged by size:
1391 452 32
XhoI C'TCGA_G
Cuts at: 0 221 1875
Size: 221 1654
XmaI C'CCGG_G
Cuts at: 0 524 1875
Size: 524 1351
XmnI GAAnn'nnTTC
Cuts at: 0 1099 1875
Size: 1099 776
Enzymes that do cut:
AcuI AflIII AleI ApaI ApaLI ApoI AseI AvaI
BanI BanII BbsI BbvCI BcgI BclI BfrBI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BsiHKAI Bsp1286I BspEI
BspMI BsrBI BsrDI BstAPI BtgI BtsI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HindIII MmeI MscI MslI NcoI NsiI NspI PciI
PpuMI PsiI PspOMI PstI SacI SapI SfcI SmaI
SmlI SpeI StuI StyI TaqII TatI TstI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI AscI AsiSI AvrII BaeI
BamHI BbeI BciVI BglII BlpI BmtI BsaHI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspHI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SphI SrfI SspI SwaI Tth111I XbaI
XcmI ZraI