Restriction map for
pF1KB7857
(Linear) (Six-base) MAPSORT of: KIBB7857.seq Check: 1967 from: 1 to: 1659
KIBB7857.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AatII G_ACGT'C
Cuts at: 0 892 1659
Size: 892 767
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 864 1032 1200 1659
Size: 864 168 168 459
Fragments arranged by size:
864 459 168 168
AflIII A'CryG_T
Cuts at: 0 938 1659
Size: 938 721
AleI CACnn'nnGTG
Cuts at: 0 412 1091 1659
Size: 412 679 568
Fragments arranged by size:
679 568 412
AlwNI CAG_nnn'CTG
Cuts at: 0 636 1335 1659
Size: 636 699 324
Fragments arranged by size:
699 636 324
ApaI G_GGCC'C
Cuts at: 0 72 1630 1659
Size: 72 1558 29
Fragments arranged by size:
1558 72 29
ApaLI G'TGCA_C
Cuts at: 0 593 1252 1659
Size: 593 659 407
Fragments arranged by size:
659 593 407
AvaI C'yCGr_G
Cuts at: 0 392 505 1623 1659
Size: 392 113 1118 36
Fragments arranged by size:
1118 392 113 36
BamHI G'GATC_C
Cuts at: 0 1502 1659
Size: 1502 157
BanI G'GyrC_C
Cuts at: 0 340 379 989 1393 1659
Size: 340 39 610 404 266
Fragments arranged by size:
610 404 340 266 39
BanII G_rGCy'C
Cuts at: 0 50 72 176 719 808 1228 1613 1630
Size: 50 22 104 543 89 420 385 17
Cuts at: 1630 1659
Size: 29
Fragments arranged by size:
543 420 385 104 89 50 29 22
17
BbeI G_GCGC'C
Cuts at: 0 1397 1659
Size: 1397 262
BbsI GAAGACnn'nnnn_
Cuts at: 0 318 1525 1659
Size: 318 1207 134
Fragments arranged by size:
1207 318 134
BbvCI CC'TCA_GC
Cuts at: 0 1066 1659
Size: 1066 593
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 777 811 1365 1399 1659
Size: 777 34 554 34 260
Fragments arranged by size:
777 554 260 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 67 579 1659
Size: 67 512 1080
Fragments arranged by size:
1080 512 67
BlpI GC'TnA_GC
Cuts at: 0 1405 1659
Size: 1405 254
Bme1580I G_kGCm'C
Cuts at: 0 72 597 946 1256 1630 1659
Size: 72 525 349 310 374 29
Fragments arranged by size:
525 374 349 310 72 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 179 438 858 1659
Size: 179 259 420 801
Fragments arranged by size:
801 420 259 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 264 293 658 1659
Size: 178 86 29 365 1001
Fragments arranged by size:
1001 365 178 86 29
Bpu10I CC'TnA_GC
Cuts at: 0 450 496 520 575 1066 1659
Size: 450 46 24 55 491 593
Fragments arranged by size:
593 491 450 55 46 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 1659
Size: 802 857
BsaHI Gr'CG_yC
Cuts at: 0 512 571 889 931 1098 1394 1659
Size: 512 59 318 42 167 296 265
Fragments arranged by size:
512 318 296 265 167 59 42
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 184 691 805 966 1218 1659
Size: 52 132 507 114 161 252 441
Fragments arranged by size:
507 441 252 161 132 114 52
BseYI C'CCAG_C
Cuts at: 0 117 552 735 754 1322 1629 1659
Size: 117 435 183 19 568 307 30
Fragments arranged by size:
568 435 307 183 117 30 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 804 1321 1531 1659
Size: 695 109 517 210 128
Fragments arranged by size:
695 517 210 128 109
BsiEI CG_ry'CG
Cuts at: 0 654 1010 1293 1659
Size: 654 356 283 366
Fragments arranged by size:
654 366 356 283
BsiHKAI G_wGCw'C
Cuts at: 0 597 808 1256 1659
Size: 597 211 448 403
Fragments arranged by size:
597 448 403 211
BsiWI C'GTAC_G
Cuts at: 0 1165 1659
Size: 1165 494
BsmI GAATG_Cn'
Cuts at: 0 778 1418 1659
Size: 778 640 241
Fragments arranged by size:
778 640 241
BsmBI CGTCTCn'nnnn_
Cuts at: 0 922 1644 1659
Size: 922 722 15
Fragments arranged by size:
922 722 15
Bsp1286I G_dGCh'C
Cuts at: 0 50 72 176 597 719 808 946 1228
Size: 50 22 104 421 122 89 138 282
Cuts at: 1228 1256 1613 1630 1659
Size: 28 357 17 29
Fragments arranged by size:
421 357 282 138 122 104 89 50
29 28 22 17
BsrBI CCG'CTC
Cuts at: 0 579 846 1350 1659
Size: 579 267 504 309
Fragments arranged by size:
579 504 309 267
BsrFI r'CCGG_y
Cuts at: 0 337 646 1412 1510 1659
Size: 337 309 766 98 149
Fragments arranged by size:
766 337 309 149 98
BssHII G'CGCG_C
Cuts at: 0 1196 1659
Size: 1196 463
BssSI C'ACGA_G
Cuts at: 0 140 284 1159 1659
Size: 140 144 875 500
Fragments arranged by size:
875 500 144 140
BstAPI GCAn_nnn'nTGC
Cuts at: 0 549 782 1659
Size: 549 233 877
Fragments arranged by size:
877 549 233
BstBI TT'CG_AA
Cuts at: 0 770 1659
Size: 770 889
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1093 1659
Size: 1093 566
BstYI r'GATC_y
Cuts at: 0 1082 1502 1659
Size: 1082 420 157
Fragments arranged by size:
1082 420 157
BtgI C'CryG_G
Cuts at: 0 407 1053 1659
Size: 407 646 606
Fragments arranged by size:
646 606 407
BtsI GCAGTG_nn'
Cuts at: 0 462 1459 1659
Size: 462 997 200
Fragments arranged by size:
997 462 200
DraIII CAC_nnn'GTG
Cuts at: 0 1120 1659
Size: 1120 539
EaeI y'GGCC_r
Cuts at: 0 280 708 1007 1122 1290 1458 1659
Size: 280 428 299 115 168 168 201
Fragments arranged by size:
428 299 280 201 168 168 115
EagI C'GGCC_G
Cuts at: 0 1007 1290 1659
Size: 1007 283 369
Fragments arranged by size:
1007 369 283
EarI CTCTTCn'nnn_
Cuts at: 0 752 796 1492 1659
Size: 752 44 696 167
Fragments arranged by size:
752 696 167 44
EcoICRI GAG'CTC
Cuts at: 0 806 1659
Size: 806 853
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 264 293 658 864 1032 1200 1659
Size: 178 86 29 365 206 168 168 459
Fragments arranged by size:
459 365 206 178 168 168 86 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 222 1659
Size: 222 1437
EcoO109I rG'GnC_Cy
Cuts at: 0 68 69 603 633 1659
Size: 68 1 534 30 1026
Fragments arranged by size:
1026 534 68 30 1
FspI TGC'GCA
Cuts at: 0 1113 1659
Size: 1113 546
HaeII r_GCGC'y
Cuts at: 0 629 1397 1479 1659
Size: 629 768 82 180
Fragments arranged by size:
768 629 180 82
KasI G'GCGC_C
Cuts at: 0 1393 1659
Size: 1393 266
MluI A'CGCG_T
Cuts at: 0 938 1659
Size: 938 721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 833 1085 1337 1659
Size: 177 656 252 252 322
Fragments arranged by size:
656 322 252 252 177
MscI TGG'CCA
Cuts at: 0 282 710 1659
Size: 282 428 949
Fragments arranged by size:
949 428 282
MslI CAynn'nnrTG
Cuts at: 0 412 1091 1659
Size: 412 679 568
Fragments arranged by size:
679 568 412
MspA1I CmG'CkG
Cuts at: 0 923 1025 1361 1651 1659
Size: 923 102 336 290 8
Fragments arranged by size:
923 336 290 102 8
NaeI GCC'GGC
Cuts at: 0 648 1659
Size: 648 1011
NarI GG'CG_CC
Cuts at: 0 1394 1659
Size: 1394 265
NgoMIV G'CCGG_C
Cuts at: 0 646 1659
Size: 646 1013
NotI GC'GGCC_GC
Cuts at: 0 1290 1659
Size: 1290 369
NspI r_CATG'y
Cuts at: 0 547 1659
Size: 547 1112
PasI CC'CwG_GG
Cuts at: 0 176 607 1659
Size: 176 431 1052
Fragments arranged by size:
1052 431 176
PflMI CCAn_nnn'nTGG
Cuts at: 0 636 1659
Size: 636 1023
PfoI T'CCnGG_A
Cuts at: 0 50 208 241 1659
Size: 50 158 33 1418
Fragments arranged by size:
1418 158 50 33
PpuMI rG'GwC_Cy
Cuts at: 0 603 633 1659
Size: 603 30 1026
Fragments arranged by size:
1026 603 30
PspOMI G'GGCC_C
Cuts at: 0 68 1626 1659
Size: 68 1558 33
Fragments arranged by size:
1558 68 33
PstI C_TGCA'G
Cuts at: 0 368 1659
Size: 368 1291
SacI G_AGCT'C
Cuts at: 0 808 1659
Size: 808 851
SapI GCTCTTCn'nnn_
Cuts at: 0 752 796 1659
Size: 752 44 863
Fragments arranged by size:
863 752 44
SfcI C'TryA_G
Cuts at: 0 219 364 1659
Size: 219 145 1295
Fragments arranged by size:
1295 219 145
SfoI GGC'GCC
Cuts at: 0 1395 1659
Size: 1395 264
SgrAI Cr'CCGG_yG
Cuts at: 0 1412 1659
Size: 1412 247
SmlI C'TyrA_G
Cuts at: 0 817 1659
Size: 817 842
SphI G_CATG'C
Cuts at: 0 547 1659
Size: 547 1112
StuI AGG'CCT
Cuts at: 0 501 877 961 1045 1381 1659
Size: 501 376 84 84 336 278
Fragments arranged by size:
501 376 336 278 84 84
StyI C'CwwG_G
Cuts at: 0 1388 1659
Size: 1388 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 661 847 1336 1659
Size: 661 186 489 323
Fragments arranged by size:
661 489 323 186
TatI w'GTAC_w
Cuts at: 0 23 1659
Size: 23 1636
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 275 307 587 619 1659
Size: 275 32 280 32 1040
Fragments arranged by size:
1040 280 275 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 160 1659
Size: 160 1499
XmnI GAAnn'nnTTC
Cuts at: 0 766 1018 1186 1270 1438 1659
Size: 766 252 168 84 168 221
Fragments arranged by size:
766 252 221 168 168 84
ZraI GAC'GTC
Cuts at: 0 890 1659
Size: 890 769
Enzymes that do cut:
AatII AcuI AflIII AleI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FspI HaeII KasI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NotI NspI PasI
PflMI PfoI PpuMI PspOMI PstI SacI SapI SfcI
SfoI SgrAI SmlI SphI StuI StyI TaqII TatI
TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BciVI BclI BfrBI BglII BmgBI BmtI BplI BsaI
BsaAI BsaBI BsaWI BsaXI BspEI BspHI BspMI BsrDI
BsrGI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EciI EcoRI EcoRV FalI FseI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI NcoI NdeI NheI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SmaI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI