Restriction map for pF1KB7864
 (Linear) (Six-base) MAPSORT of: KIBB7864.seq  Check: 8191  from: 1  to: 1893



KIBB7864.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     936    1739    1835    1893 
   Size:        936     803      96      58

  Fragments arranged by size: 

                 936     803      96      58
 
AccI GT'mk_AC

Cuts at:      0     791    1033    1893 
   Size:        791     242     860

  Fragments arranged by size: 

                 860     791     242
 
AflIII A'CryG_T

Cuts at:      0     851    1893 
   Size:        851    1042
 
AleI CACnn'nnGTG

Cuts at:      0     781    1893 
   Size:        781    1112
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1801    1893 
   Size:       1801      92
 
AlwNI CAG_nnn'CTG

Cuts at:      0      87     639     759    1117    1401    1639    1731    1893 
   Size:         87     552     120     358     284     238      92     162

  Fragments arranged by size: 

                 552     358     284     238     162     120      92      87
            
 
ApaI G_GGCC'C

Cuts at:      0     104     622     866    1126    1893 
   Size:        104     518     244     260     767

  Fragments arranged by size: 

                 767     518     260     244     104
 
AvaI C'yCGr_G

Cuts at:      0     200     882    1519    1893 
   Size:        200     682     637     374

  Fragments arranged by size: 

                 682     637     374     200
 
BanI G'GyrC_C

Cuts at:      0    1288    1893 
   Size:       1288     605
 
BanII G_rGCy'C

Cuts at:      0      35     104     208     566     622     766     866    1073 
   Size:         35      69     104     358      56     144     100     207

Cuts at:   1073    1126    1495    1801    1893 
   Size:         53     369     306      92

  Fragments arranged by size: 

                 369     358     306     207     144     104     100      92
                  69      56      53      35
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     242     469    1617    1893 
   Size:        242     227    1148     276

  Fragments arranged by size: 

                1148     276     242     227
 
BbvCI CC'TCA_GC

Cuts at:      0     274    1893 
   Size:        274    1619
 
BfrBI ATG'CAT

Cuts at:      0     722    1893 
   Size:        722    1171
 
BglI GCCn_nnn'nGGC

Cuts at:      0     186    1477    1893 
   Size:        186    1291     416

  Fragments arranged by size: 

                1291     416     186
 
BlpI GC'TnA_GC

Cuts at:      0      61     173    1481    1750    1893 
   Size:         61     112    1308     269     143

  Fragments arranged by size: 

                1308     269     143     112      61
 
Bme1580I G_kGCm'C

Cuts at:      0     104     454     622     866    1126    1893 
   Size:        104     350     168     244     260     767

  Fragments arranged by size: 

                 767     350     260     244     168     104
 
BmgBI CAC'GTC

Cuts at:      0    1698    1893 
   Size:       1698     195
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      97     935    1524    1893 
   Size:         97     838     589     369

  Fragments arranged by size: 

                 838     589     369      97
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1275    1893 
   Size:       1275     618
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64      70     137     286    1087    1632    1758    1791 
   Size:         64       6      67     149     801     545     126      33

Cuts at:   1791    1893 
   Size:        102

  Fragments arranged by size: 

                 801     545     149     126     102      67      64      33
                   6
 
Bpu10I CC'TnA_GC

Cuts at:      0     274    1588    1893 
   Size:        274    1314     305

  Fragments arranged by size: 

                1314     305     274
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     987    1893 
   Size:        987     906
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     307    1113    1849    1893 
   Size:        307     806     736      44

  Fragments arranged by size: 

                 806     736     307      44
 
BsaAI yAC'GTr

Cuts at:      0     854    1893 
   Size:        854    1039
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     319     349     591     621    1893 
   Size:        319      30     242      30    1272

  Fragments arranged by size: 

                1272     319     242      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     298     694     715    1893 
   Size:        298     396      21    1178

  Fragments arranged by size: 

                1178     396     298      21
 
BseYI C'CCAG_C

Cuts at:      0     189     621     629     759    1072    1117    1394    1702 
   Size:        189     432       8     130     313      45     277     308

Cuts at:   1702    1893 
   Size:        191

  Fragments arranged by size: 

                 432     313     308     277     191     189     130      45
                   8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1141    1387    1459    1893 
   Size:       1141     246      72     434

  Fragments arranged by size: 

                1141     434     246      72
 
BsiEI CG_ry'CG

Cuts at:      0     159    1893 
   Size:        159    1734
 
BsiHKAI G_wGCw'C

Cuts at:      0      35    1801    1893 
   Size:         35    1766      92

  Fragments arranged by size: 

                1766      92      35
 
BsmI GAATG_Cn'

Cuts at:      0     724    1893 
   Size:        724    1169
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     211     682    1893 
   Size:        211     471    1211

  Fragments arranged by size: 

                1211     471     211
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     104     208     454     566     622     766     866 
   Size:         35      69     104     246     112      56     144     100

Cuts at:    866    1073    1126    1495    1801    1893 
   Size:        207      53     369     306      92

  Fragments arranged by size: 

                 369     306     246     207     144     112     104     100
                  92      69      56      53      35
 
BspHI T'CATG_A

Cuts at:      0    1232    1893 
   Size:       1232     661
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     374     897     936    1611    1739    1835    1893 
   Size:        374     523      39     675     128      96      58

  Fragments arranged by size: 

                 675     523     374     128      96      58      39
 
BsrBI CCG'CTC

Cuts at:      0     901    1425    1893 
   Size:        901     524     468

  Fragments arranged by size: 

                 901     524     468
 
BsrDI GCAATG_nn'

Cuts at:      0    1818    1893 
   Size:       1818      75
 
BsrFI r'CCGG_y

Cuts at:      0     809    1338    1671    1717    1737    1745    1893 
   Size:        809     529     333      46      20       8     148

  Fragments arranged by size: 

                 809     529     333     148      46      20       8
 
BsrGI T'GTAC_A

Cuts at:      0     485    1893 
   Size:        485    1408
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1563    1893 
   Size:       1563     330
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     189    1065    1782    1875    1893 
   Size:        189     876     717      93      18

  Fragments arranged by size: 

                 876     717     189      93      18
 
BstYI r'GATC_y

Cuts at:      0     635    1893 
   Size:        635    1258
 
Bsu36I CC'TnA_GG

Cuts at:      0     281    1893 
   Size:        281    1612
 
BtgI C'CryG_G

Cuts at:      0     305     334     845     951    1058    1475    1893 
   Size:        305      29     511     106     107     417     418

  Fragments arranged by size: 

                 511     418     417     305     107     106      29
 
BtsI GCAGTG_nn'

Cuts at:      0      73    1153    1893 
   Size:         73    1080     740

  Fragments arranged by size: 

                1080     740      73
 
DraIII CAC_nnn'GTG

Cuts at:      0      91     957    1050    1333    1893 
   Size:         91     866      93     283     560

  Fragments arranged by size: 

                 866     560     283      93      91
 
EaeI y'GGCC_r

Cuts at:      0     842    1469    1598    1815    1893 
   Size:        842     627     129     217      78

  Fragments arranged by size: 

                 842     627     217     129      78
 
EarI CTCTTCn'nnn_

Cuts at:      0     554    1887    1893 
   Size:        554    1333       6

  Fragments arranged by size: 

                1333     554       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     612     730    1893 
   Size:        612     118    1163

  Fragments arranged by size: 

                1163     612     118
 
EcoICRI GAG'CTC

Cuts at:      0      33    1799    1893 
   Size:         33    1766      94

  Fragments arranged by size: 

                1766      94      33
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64      70     137     286    1087    1632    1758    1791 
   Size:         64       6      67     149     801     545     126      33

Cuts at:   1791    1893 
   Size:        102

  Fragments arranged by size: 

                 801     545     149     126     102      67      64      33
                   6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     133     322     445     643     654    1893 
   Size:        133     189     123     198      11    1239

  Fragments arranged by size: 

                1239     198     189     133     123      11
 
EcoO109I rG'GnC_Cy

Cuts at:      0     100     101     124     330     618     863     886    1122 
   Size:        100       1      23     206     288     245      23     236

Cuts at:   1122    1123    1267    1710    1893 
   Size:          1     144     443     183

  Fragments arranged by size: 

                 443     288     245     236     206     183     144     100
                  23      23       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1641    1893 
   Size:       1641     252
 
HaeII r_GCGC'y

Cuts at:      0    1595    1757    1893 
   Size:       1595     162     136

  Fragments arranged by size: 

                1595     162     136
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1159    1191    1893 
   Size:       1159      32     702

  Fragments arranged by size: 

                1159     702      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     791    1862    1893 
   Size:        791    1071      31

  Fragments arranged by size: 

                1071     791      31
 
MscI TGG'CCA

Cuts at:      0     844    1471    1600    1817    1893 
   Size:        844     627     129     217      76

  Fragments arranged by size: 

                 844     627     217     129      76
 
MslI CAynn'nnrTG

Cuts at:      0     457     781    1873    1893 
   Size:        457     324    1092      20

  Fragments arranged by size: 

                1092     457     324      20
 
MspA1I CmG'CkG

Cuts at:      0      21     390     860    1010    1117    1401    1485    1893 
   Size:         21     369     470     150     107     284      84     408

  Fragments arranged by size: 

                 470     408     369     284     150     107      84      21
            
 
NaeI GCC'GGC

Cuts at:      0    1673    1719    1739    1893 
   Size:       1673      46      20     154

  Fragments arranged by size: 

                1673     154      46      20
 
NcoI C'CATG_G

Cuts at:      0     845    1475    1893 
   Size:        845     630     418

  Fragments arranged by size: 

                 845     630     418
 
NgoMIV G'CCGG_C

Cuts at:      0    1671    1717    1737    1893 
   Size:       1671      46      20     156

  Fragments arranged by size: 

                1671     156      46      20
 
NsiI A_TGCA'T

Cuts at:      0     724    1893 
   Size:        724    1169
 
PasI CC'CwG_GG

Cuts at:      0     871    1263    1893 
   Size:        871     392     630

  Fragments arranged by size: 

                 871     630     392
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      87    1893 
   Size:         87    1806
 
PpuMI rG'GwC_Cy

Cuts at:      0     330     886    1267    1710    1893 
   Size:        330     556     381     443     183

  Fragments arranged by size: 

                 556     443     381     330     183
 
PspOMI G'GGCC_C

Cuts at:      0     100     618     862    1122    1893 
   Size:        100     518     244     260     771

  Fragments arranged by size: 

                 771     518     260     244     100
 
PstI C_TGCA'G

Cuts at:      0      26     326     371     993    1490    1736    1893 
   Size:         26     300      45     622     497     246     157

  Fragments arranged by size: 

                 622     497     300     246     157      45      26
 
PvuII CAG'CTG

Cuts at:      0      21    1117    1485    1893 
   Size:         21    1096     368     408

  Fragments arranged by size: 

                1096     408     368      21
 
SacI G_AGCT'C

Cuts at:      0      35    1801    1893 
   Size:         35    1766      92

  Fragments arranged by size: 

                1766      92      35
 
SapI GCTCTTCn'nnn_

Cuts at:      0     554    1893 
   Size:        554    1339
 
SexAI A'CCwGG_T

Cuts at:      0     490    1893 
   Size:        490    1403
 
SfcI C'TryA_G

Cuts at:      0      22     322     367     989    1486    1506    1732    1893 
   Size:         22     300      45     622     497      20     226     161

  Fragments arranged by size: 

                 622     497     300     226     161      45      22      20
            
 
SmaI CCC'GGG

Cuts at:      0     884    1893 
   Size:        884    1009
 
SmlI C'TyrA_G

Cuts at:      0    1002    1893 
   Size:       1002     891
 
StuI AGG'CCT

Cuts at:      0     649    1184    1645    1893 
   Size:        649     535     461     248

  Fragments arranged by size: 

                 649     535     461     248
 
StyI C'CwwG_G

Cuts at:      0     845    1475    1893 
   Size:        845     630     418

  Fragments arranged by size: 

                 845     630     418
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      79     264     547    1552    1885    1893 
   Size:         79     185     283    1005     333       8

  Fragments arranged by size: 

                1005     333     283     185      79       8
 
TatI w'GTAC_w

Cuts at:      0     485    1090    1532    1893 
   Size:        485     605     442     361

  Fragments arranged by size: 

                 605     485     442     361
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     319     351    1036    1068    1893 
   Size:        319      32     685      32     825

  Fragments arranged by size: 

                 825     685     319      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1029    1893 
   Size:       1029     864
 
XmaI C'CCGG_G

Cuts at:      0     882    1893 
   Size:        882    1011
 
XmnI GAAnn'nnTTC

Cuts at:      0     607    1462    1893 
   Size:        607     855     431

  Fragments arranged by size: 

                 855     607     431
 

 Enzymes that do cut:

     AarI     AccI   AflIII     AleI     AlfI    AlwNI     ApaI     AvaI
     BanI    BanII     BbsI    BbvCI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI
    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NsiI
     PasI    PflMI    PpuMI   PspOMI     PstI    PvuII     SacI     SapI
    SexAI     SfcI     SmaI     SmlI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BcgI    BciVI     BclI    BglII     BmtI    BsaBI
    BsaHI    BsaWI    BsiWI    BspEI   BssHII    BssSI    BstBI   BstEII
  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI
     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     XbaI     XcmI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com