(Linear) (Six-base) MAPSORT of: KIBB7864.seq Check: 8191 from: 1 to: 1893 KIBB7864.orf With 182 enzymes: SgfI * October 7, 2009 14:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 936 1739 1835 1893 Size: 936 803 96 58 Fragments arranged by size: 936 803 96 58 AccI GT'mk_AC Cuts at: 0 791 1033 1893 Size: 791 242 860 Fragments arranged by size: 860 791 242 AflIII A'CryG_T Cuts at: 0 851 1893 Size: 851 1042 AleI CACnn'nnGTG Cuts at: 0 781 1893 Size: 781 1112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1801 1893 Size: 1801 92 AlwNI CAG_nnn'CTG Cuts at: 0 87 639 759 1117 1401 1639 1731 1893 Size: 87 552 120 358 284 238 92 162 Fragments arranged by size: 552 358 284 238 162 120 92 87 ApaI G_GGCC'C Cuts at: 0 104 622 866 1126 1893 Size: 104 518 244 260 767 Fragments arranged by size: 767 518 260 244 104 AvaI C'yCGr_G Cuts at: 0 200 882 1519 1893 Size: 200 682 637 374 Fragments arranged by size: 682 637 374 200 BanI G'GyrC_C Cuts at: 0 1288 1893 Size: 1288 605 BanII G_rGCy'C Cuts at: 0 35 104 208 566 622 766 866 1073 Size: 35 69 104 358 56 144 100 207 Cuts at: 1073 1126 1495 1801 1893 Size: 53 369 306 92 Fragments arranged by size: 369 358 306 207 144 104 100 92 69 56 53 35 BbsI GAAGACnn'nnnn_ Cuts at: 0 242 469 1617 1893 Size: 242 227 1148 276 Fragments arranged by size: 1148 276 242 227 BbvCI CC'TCA_GC Cuts at: 0 274 1893 Size: 274 1619 BfrBI ATG'CAT Cuts at: 0 722 1893 Size: 722 1171 BglI GCCn_nnn'nGGC Cuts at: 0 186 1477 1893 Size: 186 1291 416 Fragments arranged by size: 1291 416 186 BlpI GC'TnA_GC Cuts at: 0 61 173 1481 1750 1893 Size: 61 112 1308 269 143 Fragments arranged by size: 1308 269 143 112 61 Bme1580I G_kGCm'C Cuts at: 0 104 454 622 866 1126 1893 Size: 104 350 168 244 260 767 Fragments arranged by size: 767 350 260 244 168 104 BmgBI CAC'GTC Cuts at: 0 1698 1893 Size: 1698 195 BmrI ACTGGGnnnn_n' Cuts at: 0 97 935 1524 1893 Size: 97 838 589 369 Fragments arranged by size: 838 589 369 97 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1275 1893 Size: 1275 618 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 70 137 286 1087 1632 1758 1791 Size: 64 6 67 149 801 545 126 33 Cuts at: 1791 1893 Size: 102 Fragments arranged by size: 801 545 149 126 102 67 64 33 6 Bpu10I CC'TnA_GC Cuts at: 0 274 1588 1893 Size: 274 1314 305 Fragments arranged by size: 1314 305 274 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 987 1893 Size: 987 906 BsaI GGTCTCn'nnnn_ Cuts at: 0 307 1113 1849 1893 Size: 307 806 736 44 Fragments arranged by size: 806 736 307 44 BsaAI yAC'GTr Cuts at: 0 854 1893 Size: 854 1039 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 319 349 591 621 1893 Size: 319 30 242 30 1272 Fragments arranged by size: 1272 319 242 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 298 694 715 1893 Size: 298 396 21 1178 Fragments arranged by size: 1178 396 298 21 BseYI C'CCAG_C Cuts at: 0 189 621 629 759 1072 1117 1394 1702 Size: 189 432 8 130 313 45 277 308 Cuts at: 1702 1893 Size: 191 Fragments arranged by size: 432 313 308 277 191 189 130 45 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1141 1387 1459 1893 Size: 1141 246 72 434 Fragments arranged by size: 1141 434 246 72 BsiEI CG_ry'CG Cuts at: 0 159 1893 Size: 159 1734 BsiHKAI G_wGCw'C Cuts at: 0 35 1801 1893 Size: 35 1766 92 Fragments arranged by size: 1766 92 35 BsmI GAATG_Cn' Cuts at: 0 724 1893 Size: 724 1169 BsmBI CGTCTCn'nnnn_ Cuts at: 0 211 682 1893 Size: 211 471 1211 Fragments arranged by size: 1211 471 211 Bsp1286I G_dGCh'C Cuts at: 0 35 104 208 454 566 622 766 866 Size: 35 69 104 246 112 56 144 100 Cuts at: 866 1073 1126 1495 1801 1893 Size: 207 53 369 306 92 Fragments arranged by size: 369 306 246 207 144 112 104 100 92 69 56 53 35 BspHI T'CATG_A Cuts at: 0 1232 1893 Size: 1232 661 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 374 897 936 1611 1739 1835 1893 Size: 374 523 39 675 128 96 58 Fragments arranged by size: 675 523 374 128 96 58 39 BsrBI CCG'CTC Cuts at: 0 901 1425 1893 Size: 901 524 468 Fragments arranged by size: 901 524 468 BsrDI GCAATG_nn' Cuts at: 0 1818 1893 Size: 1818 75 BsrFI r'CCGG_y Cuts at: 0 809 1338 1671 1717 1737 1745 1893 Size: 809 529 333 46 20 8 148 Fragments arranged by size: 809 529 333 148 46 20 8 BsrGI T'GTAC_A Cuts at: 0 485 1893 Size: 485 1408 BstAPI GCAn_nnn'nTGC Cuts at: 0 1563 1893 Size: 1563 330 BstXI CCAn_nnnn'nTGG Cuts at: 0 189 1065 1782 1875 1893 Size: 189 876 717 93 18 Fragments arranged by size: 876 717 189 93 18 BstYI r'GATC_y Cuts at: 0 635 1893 Size: 635 1258 Bsu36I CC'TnA_GG Cuts at: 0 281 1893 Size: 281 1612 BtgI C'CryG_G Cuts at: 0 305 334 845 951 1058 1475 1893 Size: 305 29 511 106 107 417 418 Fragments arranged by size: 511 418 417 305 107 106 29 BtsI GCAGTG_nn' Cuts at: 0 73 1153 1893 Size: 73 1080 740 Fragments arranged by size: 1080 740 73 DraIII CAC_nnn'GTG Cuts at: 0 91 957 1050 1333 1893 Size: 91 866 93 283 560 Fragments arranged by size: 866 560 283 93 91 EaeI y'GGCC_r Cuts at: 0 842 1469 1598 1815 1893 Size: 842 627 129 217 78 Fragments arranged by size: 842 627 217 129 78 EarI CTCTTCn'nnn_ Cuts at: 0 554 1887 1893 Size: 554 1333 6 Fragments arranged by size: 1333 554 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 612 730 1893 Size: 612 118 1163 Fragments arranged by size: 1163 612 118 EcoICRI GAG'CTC Cuts at: 0 33 1799 1893 Size: 33 1766 94 Fragments arranged by size: 1766 94 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 70 137 286 1087 1632 1758 1791 Size: 64 6 67 149 801 545 126 33 Cuts at: 1791 1893 Size: 102 Fragments arranged by size: 801 545 149 126 102 67 64 33 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 133 322 445 643 654 1893 Size: 133 189 123 198 11 1239 Fragments arranged by size: 1239 198 189 133 123 11 EcoO109I rG'GnC_Cy Cuts at: 0 100 101 124 330 618 863 886 1122 Size: 100 1 23 206 288 245 23 236 Cuts at: 1122 1123 1267 1710 1893 Size: 1 144 443 183 Fragments arranged by size: 443 288 245 236 206 183 144 100 23 23 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1641 1893 Size: 1641 252 HaeII r_GCGC'y Cuts at: 0 1595 1757 1893 Size: 1595 162 136 Fragments arranged by size: 1595 162 136 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1159 1191 1893 Size: 1159 32 702 Fragments arranged by size: 1159 702 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 791 1862 1893 Size: 791 1071 31 Fragments arranged by size: 1071 791 31 MscI TGG'CCA Cuts at: 0 844 1471 1600 1817 1893 Size: 844 627 129 217 76 Fragments arranged by size: 844 627 217 129 76 MslI CAynn'nnrTG Cuts at: 0 457 781 1873 1893 Size: 457 324 1092 20 Fragments arranged by size: 1092 457 324 20 MspA1I CmG'CkG Cuts at: 0 21 390 860 1010 1117 1401 1485 1893 Size: 21 369 470 150 107 284 84 408 Fragments arranged by size: 470 408 369 284 150 107 84 21 NaeI GCC'GGC Cuts at: 0 1673 1719 1739 1893 Size: 1673 46 20 154 Fragments arranged by size: 1673 154 46 20 NcoI C'CATG_G Cuts at: 0 845 1475 1893 Size: 845 630 418 Fragments arranged by size: 845 630 418 NgoMIV G'CCGG_C Cuts at: 0 1671 1717 1737 1893 Size: 1671 46 20 156 Fragments arranged by size: 1671 156 46 20 NsiI A_TGCA'T Cuts at: 0 724 1893 Size: 724 1169 PasI CC'CwG_GG Cuts at: 0 871 1263 1893 Size: 871 392 630 Fragments arranged by size: 871 630 392 PflMI CCAn_nnn'nTGG Cuts at: 0 87 1893 Size: 87 1806 PpuMI rG'GwC_Cy Cuts at: 0 330 886 1267 1710 1893 Size: 330 556 381 443 183 Fragments arranged by size: 556 443 381 330 183 PspOMI G'GGCC_C Cuts at: 0 100 618 862 1122 1893 Size: 100 518 244 260 771 Fragments arranged by size: 771 518 260 244 100 PstI C_TGCA'G Cuts at: 0 26 326 371 993 1490 1736 1893 Size: 26 300 45 622 497 246 157 Fragments arranged by size: 622 497 300 246 157 45 26 PvuII CAG'CTG Cuts at: 0 21 1117 1485 1893 Size: 21 1096 368 408 Fragments arranged by size: 1096 408 368 21 SacI G_AGCT'C Cuts at: 0 35 1801 1893 Size: 35 1766 92 Fragments arranged by size: 1766 92 35 SapI GCTCTTCn'nnn_ Cuts at: 0 554 1893 Size: 554 1339 SexAI A'CCwGG_T Cuts at: 0 490 1893 Size: 490 1403 SfcI C'TryA_G Cuts at: 0 22 322 367 989 1486 1506 1732 1893 Size: 22 300 45 622 497 20 226 161 Fragments arranged by size: 622 497 300 226 161 45 22 20 SmaI CCC'GGG Cuts at: 0 884 1893 Size: 884 1009 SmlI C'TyrA_G Cuts at: 0 1002 1893 Size: 1002 891 StuI AGG'CCT Cuts at: 0 649 1184 1645 1893 Size: 649 535 461 248 Fragments arranged by size: 649 535 461 248 StyI C'CwwG_G Cuts at: 0 845 1475 1893 Size: 845 630 418 Fragments arranged by size: 845 630 418 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 79 264 547 1552 1885 1893 Size: 79 185 283 1005 333 8 Fragments arranged by size: 1005 333 283 185 79 8 TatI w'GTAC_w Cuts at: 0 485 1090 1532 1893 Size: 485 605 442 361 Fragments arranged by size: 605 485 442 361 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 319 351 1036 1068 1893 Size: 319 32 685 32 825 Fragments arranged by size: 825 685 319 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1029 1893 Size: 1029 864 XmaI C'CCGG_G Cuts at: 0 882 1893 Size: 882 1011 XmnI GAAnn'nnTTC Cuts at: 0 607 1462 1893 Size: 607 855 431 Fragments arranged by size: 855 607 431 Enzymes that do cut: AarI AccI AflIII AleI AlfI AlwNI ApaI AvaI BanI BanII BbsI BbvCI BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI PasI PflMI PpuMI PspOMI PstI PvuII SacI SapI SexAI SfcI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BglII BmtI BsaBI BsaHI BsaWI BsiWI BspEI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI XhoI ZraI