Restriction map for
pF1KB7868
(Linear) (Six-base) MAPSORT of: KIBB7868.seq Check: 2428 from: 1 to: 1902
KIBB7868.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 0 893 1902
Size: 893 1009
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 1902
Size: 609 1293
AflIII A'CryG_T
Cuts at: 0 96 1836 1902
Size: 96 1740 66
Fragments arranged by size:
1740 96 66
AleI CACnn'nnGTG
Cuts at: 0 1378 1902
Size: 1378 524
AlwNI CAG_nnn'CTG
Cuts at: 0 85 153 682 733 1278 1902
Size: 85 68 529 51 545 624
Fragments arranged by size:
624 545 529 85 68 51
ApaLI G'TGCA_C
Cuts at: 0 426 1902
Size: 426 1476
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 85 118 1902
Size: 85 33 1784
Fragments arranged by size:
1784 85 33
BanI G'GyrC_C
Cuts at: 0 307 516 1697 1902
Size: 307 209 1181 205
Fragments arranged by size:
1181 307 209 205
BanII G_rGCy'C
Cuts at: 0 210 562 842 1100 1902
Size: 210 352 280 258 802
Fragments arranged by size:
802 352 280 258 210
BbeI G_GCGC'C
Cuts at: 0 311 1902
Size: 311 1591
BbsI GAAGACnn'nnnn_
Cuts at: 0 1275 1289 1603 1902
Size: 1275 14 314 299
Fragments arranged by size:
1275 314 299 14
BbvCI CC'TCA_GC
Cuts at: 0 349 456 1066 1902
Size: 349 107 610 836
Fragments arranged by size:
836 610 349 107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1792 1826 1902
Size: 1792 34 76
Fragments arranged by size:
1792 76 34
BclI T'GATC_A
Cuts at: 0 249 1902
Size: 249 1653
BglII A'GATC_T
Cuts at: 0 951 978 1451 1902
Size: 951 27 473 451
Fragments arranged by size:
951 473 451 27
Bme1580I G_kGCm'C
Cuts at: 0 126 430 538 1375 1902
Size: 126 304 108 837 527
Fragments arranged by size:
837 527 304 126 108
BmgBI CAC'GTC
Cuts at: 0 1376 1837 1902
Size: 1376 461 65
Fragments arranged by size:
1376 461 65
BmrI ACTGGGnnnn_n'
Cuts at: 0 17 284 897 1902
Size: 17 267 613 1005
Fragments arranged by size:
1005 613 267 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1790 1902
Size: 1790 112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 796 976 1014 1442 1902
Size: 564 232 180 38 428 460
Fragments arranged by size:
564 460 428 232 180 38
Bpu10I CC'TnA_GC
Cuts at: 0 349 456 1066 1902
Size: 349 107 610 836
Fragments arranged by size:
836 610 349 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1597 1902
Size: 1597 305
BsaI GGTCTCn'nnnn_
Cuts at: 0 840 1615 1902
Size: 840 775 287
Fragments arranged by size:
840 775 287
BsaAI yAC'GTr
Cuts at: 0 897 1509 1902
Size: 897 612 393
Fragments arranged by size:
897 612 393
BsaHI Gr'CG_yC
Cuts at: 0 308 1902
Size: 308 1594
BsaWI w'CCGG_w
Cuts at: 0 842 1902
Size: 842 1060
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1377 1407 1902
Size: 1377 30 495
Fragments arranged by size:
1377 495 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2 222 722 973 982 985 1129 1226
Size: 2 220 500 251 9 3 144 97
Cuts at: 1226 1902
Size: 676
Fragments arranged by size:
676 500 251 220 144 97 9 3
2
BseYI C'CCAG_C
Cuts at: 0 511 607 726 835 918 1902
Size: 511 96 119 109 83 984
Fragments arranged by size:
984 511 119 109 96 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1902
Size: 208 1694
BsiEI CG_ry'CG
Cuts at: 0 1243 1534 1902
Size: 1243 291 368
Fragments arranged by size:
1243 368 291
BsiHKAI G_wGCw'C
Cuts at: 0 401 430 1592 1902
Size: 401 29 1162 310
Fragments arranged by size:
1162 401 310 29
Bsp1286I G_dGCh'C
Cuts at: 0 126 210 401 430 538 562 842 1100
Size: 126 84 191 29 108 24 280 258
Cuts at: 1100 1375 1592 1902
Size: 275 217 310
Fragments arranged by size:
310 280 275 258 217 191 126 108
84 29 24
BspEI T'CCGG_A
Cuts at: 0 842 1902
Size: 842 1060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1110 1171 1902
Size: 1110 61 731
Fragments arranged by size:
1110 731 61
BsrBI CCG'CTC
Cuts at: 0 1245 1902
Size: 1245 657
BsrFI r'CCGG_y
Cuts at: 0 1502 1694 1902
Size: 1502 192 208
Fragments arranged by size:
1502 208 192
BssHII G'CGCG_C
Cuts at: 0 565 1902
Size: 565 1337
BstAPI GCAn_nnn'nTGC
Cuts at: 0 153 682 1902
Size: 153 529 1220
Fragments arranged by size:
1220 529 153
BstEII G'GTnAC_C
Cuts at: 0 114 1902
Size: 114 1788
BstXI CCAn_nnnn'nTGG
Cuts at: 0 297 1902
Size: 297 1605
BstYI r'GATC_y
Cuts at: 0 951 978 1274 1451 1902
Size: 951 27 296 177 451
Fragments arranged by size:
951 451 296 177 27
BstZ17I GTA'TAC
Cuts at: 0 894 1902
Size: 894 1008
Bsu36I CC'TnA_GG
Cuts at: 0 110 1055 1902
Size: 110 945 847
Fragments arranged by size:
945 847 110
BtgI C'CryG_G
Cuts at: 0 554 1364 1742 1902
Size: 554 810 378 160
Fragments arranged by size:
810 554 378 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 185 809 1902
Size: 185 624 1093
Fragments arranged by size:
1093 624 185
BtsI GCAGTG_nn'
Cuts at: 0 95 692 699 750 827 1364 1440 1902
Size: 95 597 7 51 77 537 76 462
Fragments arranged by size:
597 537 462 95 77 76 51 7
DraIII CAC_nnn'GTG
Cuts at: 0 417 1866 1902
Size: 417 1449 36
Fragments arranged by size:
1449 417 36
EaeI y'GGCC_r
Cuts at: 0 342 1323 1531 1814 1902
Size: 342 981 208 283 88
Fragments arranged by size:
981 342 283 208 88
EagI C'GGCC_G
Cuts at: 0 1531 1902
Size: 1531 371
EarI CTCTTCn'nnn_
Cuts at: 0 959 1218 1902
Size: 959 259 684
Fragments arranged by size:
959 684 259
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 50 1902
Size: 50 1852
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 609 796 976 1014 1442 1902
Size: 564 45 187 180 38 428 460
Fragments arranged by size:
564 460 428 187 180 45 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1178 1902
Size: 1178 724
EcoO109I rG'GnC_Cy
Cuts at: 0 255 1051 1171 1416 1902
Size: 255 796 120 245 486
Fragments arranged by size:
796 486 255 245 120
HaeII r_GCGC'y
Cuts at: 0 311 925 1695 1902
Size: 311 614 770 207
Fragments arranged by size:
770 614 311 207
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 586 618 637 669 1902
Size: 586 32 19 32 1233
Fragments arranged by size:
1233 586 32 32 19
HincII GTy'rAC
Cuts at: 0 375 989 1125 1902
Size: 375 614 136 777
Fragments arranged by size:
777 614 375 136
KasI G'GCGC_C
Cuts at: 0 307 1902
Size: 307 1595
MscI TGG'CCA
Cuts at: 0 1325 1902
Size: 1325 577
MslI CAynn'nnrTG
Cuts at: 0 1378 1902
Size: 1378 524
MspA1I CmG'CkG
Cuts at: 0 48 85 1796 1902
Size: 48 37 1711 106
Fragments arranged by size:
1711 106 48 37
NaeI GCC'GGC
Cuts at: 0 1696 1902
Size: 1696 206
NarI GG'CG_CC
Cuts at: 0 308 1902
Size: 308 1594
NcoI C'CATG_G
Cuts at: 0 554 1902
Size: 554 1348
NdeI CA'TA_TG
Cuts at: 0 1158 1902
Size: 1158 744
NgoMIV G'CCGG_C
Cuts at: 0 1694 1902
Size: 1694 208
NspI r_CATG'y
Cuts at: 0 100 1263 1497 1518 1902
Size: 100 1163 234 21 384
Fragments arranged by size:
1163 384 234 100 21
PasI CC'CwG_GG
Cuts at: 0 632 1902
Size: 632 1270
PciI A'CATG_T
Cuts at: 0 96 1902
Size: 96 1806
PflMI CCAn_nnn'nTGG
Cuts at: 0 1278 1866 1902
Size: 1278 588 36
Fragments arranged by size:
1278 588 36
PfoI T'CCnGG_A
Cuts at: 0 523 1776 1902
Size: 523 1253 126
Fragments arranged by size:
1253 523 126
PmlI CAC'GTG
Cuts at: 0 1509 1902
Size: 1509 393
PpuMI rG'GwC_Cy
Cuts at: 0 255 1171 1416 1902
Size: 255 916 245 486
Fragments arranged by size:
916 486 255 245
PstI C_TGCA'G
Cuts at: 0 90 158 486 687 1902
Size: 90 68 328 201 1215
Fragments arranged by size:
1215 328 201 90 68
PvuII CAG'CTG
Cuts at: 0 85 1902
Size: 85 1817
SanDI GG'GwC_CC
Cuts at: 0 1416 1902
Size: 1416 486
SfcI C'TryA_G
Cuts at: 0 86 154 482 683 1902
Size: 86 68 328 201 1219
Fragments arranged by size:
1219 328 201 86 68
SfoI GGC'GCC
Cuts at: 0 309 1902
Size: 309 1593
SmlI C'TyrA_G
Cuts at: 0 1612 1902
Size: 1612 290
SphI G_CATG'C
Cuts at: 0 1497 1902
Size: 1497 405
StuI AGG'CCT
Cuts at: 0 1477 1902
Size: 1477 425
StyI C'CwwG_G
Cuts at: 0 554 1253 1902
Size: 554 699 649
Fragments arranged by size:
699 649 554
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1381 1866 1902
Size: 1381 485 36
Fragments arranged by size:
1381 485 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1377 1409 1419 1451 1841 1873 1902
Size: 1377 32 10 32 390 32 29
Fragments arranged by size:
1377 390 32 32 32 29 10
Tth111I GACn'n_nGTC
Cuts at: 0 370 1902
Size: 370 1532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 126 884 1038 1902
Size: 126 758 154 864
Fragments arranged by size:
864 758 154 126
XmnI GAAnn'nnTTC
Cuts at: 0 1477 1902
Size: 1477 425
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApaLI BaeI BanI
BanII BbeI BbsI BbvCI BcgI BclI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstAPI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII KasI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmlI PpuMI PstI PvuII SanDI SfcI SfoI SmlI
SphI StuI StyI TaqII TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BamHI BciVI BfrBI BglI BlpI BmtI BsaBI
BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssSI BstBI
ClaI CspCI DraI DrdI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MmeI NheI NotI NruI NsiI PacI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI
XbaI XhoI XmaI ZraI