Restriction map for
pF1KB7869
(Linear) (Six-base) MAPSORT of: KIBB7869.seq Check: 2585 from: 1 to: 1683
KIBB7869.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 1167 1251 1683
Size: 797 370 84 432
Fragments arranged by size:
797 432 370 84
AfeI AGC'GCT
Cuts at: 0 434 1264 1441 1683
Size: 434 830 177 242
Fragments arranged by size:
830 434 242 177
AleI CACnn'nnGTG
Cuts at: 0 481 1142 1683
Size: 481 661 541
Fragments arranged by size:
661 541 481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 881 1683
Size: 881 802
AlwNI CAG_nnn'CTG
Cuts at: 0 309 1683
Size: 309 1374
ApoI r'AATT_y
Cuts at: 0 3 629 768 1275 1550 1634 1683
Size: 3 626 139 507 275 84 49
Fragments arranged by size:
626 507 275 139 84 49 3
AvaI C'yCGr_G
Cuts at: 0 283 349 1683
Size: 283 66 1334
Fragments arranged by size:
1334 283 66
BanI G'GyrC_C
Cuts at: 0 84 1683
Size: 84 1599
BanII G_rGCy'C
Cuts at: 0 152 423 1683
Size: 152 271 1260
Fragments arranged by size:
1260 271 152
BbeI G_GCGC'C
Cuts at: 0 88 1683
Size: 88 1595
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 842 1683
Size: 134 708 841
Fragments arranged by size:
841 708 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 362 396 1683
Size: 362 34 1287
Fragments arranged by size:
1287 362 34
BglI GCCn_nnn'nGGC
Cuts at: 0 654 1683
Size: 654 1029
BlpI GC'TnA_GC
Cuts at: 0 244 1683
Size: 244 1439
BmgBI CAC'GTC
Cuts at: 0 974 1683
Size: 974 709
BmrI ACTGGGnnnn_n'
Cuts at: 0 1484 1568 1683
Size: 1484 84 115
Fragments arranged by size:
1484 115 84
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 147 309 1683
Size: 147 162 1374
Fragments arranged by size:
1374 162 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 346 1683
Size: 331 15 1337
Fragments arranged by size:
1337 331 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 273 737 1033 1683
Size: 273 464 296 650
Fragments arranged by size:
650 464 296 273
BsaAI yAC'GTr
Cuts at: 0 981 1683
Size: 981 702
BsaHI Gr'CG_yC
Cuts at: 0 38 85 1683
Size: 38 47 1598
Fragments arranged by size:
1598 47 38
BsaWI w'CCGG_w
Cuts at: 0 231 739 1683
Size: 231 508 944
Fragments arranged by size:
944 508 231
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 367 412 514 1683
Size: 367 45 102 1169
Fragments arranged by size:
1169 367 102 45
BseYI C'CCAG_C
Cuts at: 0 519 1660 1683
Size: 519 1141 23
Fragments arranged by size:
1141 519 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1683
Size: 178 1505
BsiHKAI G_wGCw'C
Cuts at: 0 380 571 1683
Size: 380 191 1112
Fragments arranged by size:
1112 380 191
BsmI GAATG_Cn'
Cuts at: 0 1342 1423 1683
Size: 1342 81 260
Fragments arranged by size:
1342 260 81
Bsp1286I G_dGCh'C
Cuts at: 0 152 380 423 571 1683
Size: 152 228 43 148 1112
Fragments arranged by size:
1112 228 152 148 43
BspEI T'CCGG_A
Cuts at: 0 739 1683
Size: 739 944
BsrBI CCG'CTC
Cuts at: 0 392 1683
Size: 392 1291
BsrFI r'CCGG_y
Cuts at: 0 248 1683
Size: 248 1435
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1171 1683
Size: 1171 512
BstYI r'GATC_y
Cuts at: 0 305 1683
Size: 305 1378
BtgI C'CryG_G
Cuts at: 0 161 410 1683
Size: 161 249 1273
Fragments arranged by size:
1273 249 161
BtsI GCAGTG_nn'
Cuts at: 0 39 378 1683
Size: 39 339 1305
Fragments arranged by size:
1305 339 39
DraI TTT'AAA
Cuts at: 0 1326 1455 1683
Size: 1326 129 228
Fragments arranged by size:
1326 228 129
DraIII CAC_nnn'GTG
Cuts at: 0 969 1683
Size: 969 714
EaeI y'GGCC_r
Cuts at: 0 407 1683
Size: 407 1276
EarI CTCTTCn'nnn_
Cuts at: 0 230 1087 1420 1683
Size: 230 857 333 263
Fragments arranged by size:
857 333 263 230
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 566 1683
Size: 566 1117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 346 797 1167 1251 1683
Size: 331 15 451 370 84 432
Fragments arranged by size:
451 432 370 331 84 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 349 1683
Size: 349 1334
EcoO109I rG'GnC_Cy
Cuts at: 0 49 1683
Size: 49 1634
EcoRI G'AATT_C
Cuts at: 0 3 1275 1550 1634 1683
Size: 3 1272 275 84 49
Fragments arranged by size:
1272 275 84 49 3
FspI TGC'GCA
Cuts at: 0 551 1683
Size: 551 1132
HaeII r_GCGC'y
Cuts at: 0 88 244 436 513 687 1266 1443 1683
Size: 88 156 192 77 174 579 177 240
Fragments arranged by size:
579 240 192 177 174 156 88 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 1484 1516 1683
Size: 430 32 1022 32 167
Fragments arranged by size:
1022 430 167 32 32
HindIII A'AGCT_T
Cuts at: 0 1179 1683
Size: 1179 504
KasI G'GCGC_C
Cuts at: 0 84 1683
Size: 84 1599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1385 1647 1683
Size: 902 483 262 36
Fragments arranged by size:
902 483 262 36
MslI CAynn'nnrTG
Cuts at: 0 481 1142 1683
Size: 481 661 541
Fragments arranged by size:
661 541 481
MspA1I CmG'CkG
Cuts at: 0 163 207 1683
Size: 163 44 1476
Fragments arranged by size:
1476 163 44
NaeI GCC'GGC
Cuts at: 0 250 1683
Size: 250 1433
NarI GG'CG_CC
Cuts at: 0 85 1683
Size: 85 1598
NgoMIV G'CCGG_C
Cuts at: 0 248 1683
Size: 248 1435
PasI CC'CwG_GG
Cuts at: 0 53 453 1683
Size: 53 400 1230
Fragments arranged by size:
1230 400 53
PflMI CCAn_nnn'nTGG
Cuts at: 0 222 459 1683
Size: 222 237 1224
Fragments arranged by size:
1224 237 222
PfoI T'CCnGG_A
Cuts at: 0 308 562 1683
Size: 308 254 1121
Fragments arranged by size:
1121 308 254
PmlI CAC'GTG
Cuts at: 0 981 1683
Size: 981 702
PpuMI rG'GwC_Cy
Cuts at: 0 49 1683
Size: 49 1634
PsiI TTA'TAA
Cuts at: 0 1073 1683
Size: 1073 610
PstI C_TGCA'G
Cuts at: 0 428 541 1683
Size: 428 113 1142
Fragments arranged by size:
1142 428 113
PvuII CAG'CTG
Cuts at: 0 207 1683
Size: 207 1476
SacII CC_GC'GG
Cuts at: 0 164 1683
Size: 164 1519
SanDI GG'GwC_CC
Cuts at: 0 49 1683
Size: 49 1634
SapI GCTCTTCn'nnn_
Cuts at: 0 1420 1683
Size: 1420 263
SfcI C'TryA_G
Cuts at: 0 424 537 1683
Size: 424 113 1146
Fragments arranged by size:
1146 424 113
SfoI GGC'GCC
Cuts at: 0 86 1683
Size: 86 1597
StyI C'CwwG_G
Cuts at: 0 74 296 1683
Size: 74 222 1387
Fragments arranged by size:
1387 222 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 45 357 1683
Size: 45 312 1326
Fragments arranged by size:
1326 312 45
TatI w'GTAC_w
Cuts at: 0 1609 1683
Size: 1609 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 41 73 1604 1636 1683
Size: 41 32 1531 32 47
Fragments arranged by size:
1531 47 41 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 223 1683
Size: 223 1460
XmnI GAAnn'nnTTC
Cuts at: 0 1096 1180 1429 1513 1683
Size: 1096 84 249 84 170
Fragments arranged by size:
1096 249 170 84 84
Enzymes that do cut:
AcuI AfeI AleI AlfI AlwNI ApoI AvaI BanI
BanII BbeI BbsI BcgI BglI BlpI BmgBI BmrI
BplI BpmI BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrFI BstXI
BstYI BtgI BtsI DraI DraIII EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HindIII
KasI MmeI MslI MspA1I NaeI NarI NgoMIV PasI
PflMI PfoI PmlI PpuMI PsiI PstI PvuII SacII
SanDI SapI SfcI SfoI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BclI BfrBI BglII Bme1580I
BmtI Bpu10I BpuEI BsaBI BsaXI BsiEI BsiWI BsmBI
BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EagI
EcoICRI EcoRV FalI FseI FspAI HincII HpaI KpnI
MfeI MluI MscI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PpiI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmaI ZraI