Restriction map for
pF1KB7873
(Linear) (Six-base) MAPSORT of: KIBB7873.seq Check: 9081 from: 1 to: 1710
KIBB7873.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 84 865 1045 1054 1710
Size: 84 781 180 9 656
Fragments arranged by size:
781 656 180 84 9
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 655 1295 1710
Size: 372 283 640 415
Fragments arranged by size:
640 415 372 283
AflIII A'CryG_T
Cuts at: 0 822 1710
Size: 822 888
AleI CACnn'nnGTG
Cuts at: 0 659 749 1710
Size: 659 90 961
Fragments arranged by size:
961 659 90
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 746 778 1710
Size: 746 32 932
Fragments arranged by size:
932 746 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1046 1359 1710
Size: 1046 313 351
Fragments arranged by size:
1046 351 313
ApaI G_GGCC'C
Cuts at: 0 1290 1710
Size: 1290 420
ApaLI G'TGCA_C
Cuts at: 0 1012 1710
Size: 1012 698
AvaI C'yCGr_G
Cuts at: 0 106 987 1710
Size: 106 881 723
Fragments arranged by size:
881 723 106
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 633 666 1710
Size: 633 33 1044
Fragments arranged by size:
1044 633 33
BanI G'GyrC_C
Cuts at: 0 954 1318 1477 1710
Size: 954 364 159 233
Fragments arranged by size:
954 364 233 159
BanII G_rGCy'C
Cuts at: 0 564 869 1290 1710
Size: 564 305 421 420
Fragments arranged by size:
564 421 420 305
BbsI GAAGACnn'nnnn_
Cuts at: 0 1352 1710
Size: 1352 358
BbvCI CC'TCA_GC
Cuts at: 0 1526 1710
Size: 1526 184
BclI T'GATC_A
Cuts at: 0 1304 1710
Size: 1304 406
BglI GCCn_nnn'nGGC
Cuts at: 0 1261 1432 1654 1710
Size: 1261 171 222 56
Fragments arranged by size:
1261 222 171 56
Bme1580I G_kGCm'C
Cuts at: 0 682 772 1016 1101 1290 1323 1710
Size: 682 90 244 85 189 33 387
Fragments arranged by size:
682 387 244 189 90 85 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 1409 1710
Size: 1409 301
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1185 1710
Size: 1185 525
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 1132 1630 1702 1710
Size: 433 699 498 72 8
Fragments arranged by size:
699 498 433 72 8
Bpu10I CC'TnA_GC
Cuts at: 0 1526 1710
Size: 1526 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1180 1518 1710
Size: 1180 338 192
Fragments arranged by size:
1180 338 192
BsaI GGTCTCn'nnnn_
Cuts at: 0 713 1710
Size: 713 997
BsaAI yAC'GTr
Cuts at: 0 915 1008 1710
Size: 915 93 702
Fragments arranged by size:
915 702 93
BsaHI Gr'CG_yC
Cuts at: 0 519 1710
Size: 519 1191
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 700 1138 1195 1645 1710
Size: 700 438 57 450 65
Fragments arranged by size:
700 450 438 65 57
BseYI C'CCAG_C
Cuts at: 0 244 598 1251 1322 1385 1710
Size: 244 354 653 71 63 325
Fragments arranged by size:
653 354 325 244 71 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 205 413 714 1624 1710
Size: 1 204 208 301 910 86
Fragments arranged by size:
910 301 208 204 86 1
BsiHKAI G_wGCw'C
Cuts at: 0 325 564 1016 1710
Size: 325 239 452 694
Fragments arranged by size:
694 452 325 239
BsmI GAATG_Cn'
Cuts at: 0 490 1710
Size: 490 1220
BsmBI CGTCTCn'nnnn_
Cuts at: 0 526 1710
Size: 526 1184
Bsp1286I G_dGCh'C
Cuts at: 0 325 564 682 772 869 1016 1101 1290
Size: 325 239 118 90 97 147 85 189
Cuts at: 1290 1323 1710
Size: 33 387
Fragments arranged by size:
387 325 239 189 147 118 97 90
85 33
BspHI T'CATG_A
Cuts at: 0 438 1710
Size: 438 1272
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 84 731 865 1045 1054 1317 1358 1710
Size: 84 647 134 180 9 263 41 352
Fragments arranged by size:
647 352 263 180 134 84 41 9
BsrBI CCG'CTC
Cuts at: 0 535 791 1710
Size: 535 256 919
Fragments arranged by size:
919 535 256
BsrDI GCAATG_nn'
Cuts at: 0 1595 1710
Size: 1595 115
BsrFI r'CCGG_y
Cuts at: 0 1061 1710
Size: 1061 649
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1046 1710
Size: 1046 664
BstEII G'GTnAC_C
Cuts at: 0 24 571 1380 1710
Size: 24 547 809 330
Fragments arranged by size:
809 547 330 24
BstXI CCAn_nnnn'nTGG
Cuts at: 0 252 1377 1644 1710
Size: 252 1125 267 66
Fragments arranged by size:
1125 267 252 66
Bsu36I CC'TnA_GG
Cuts at: 0 714 1710
Size: 714 996
BtgI C'CryG_G
Cuts at: 0 879 1101 1118 1460 1710
Size: 879 222 17 342 250
Fragments arranged by size:
879 342 250 222 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1279 1710
Size: 1279 431
BtsI GCAGTG_nn'
Cuts at: 0 281 407 1020 1101 1607 1649 1710
Size: 281 126 613 81 506 42 61
Fragments arranged by size:
613 506 281 126 81 61 42
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 495 530 1710
Size: 495 35 1180
Fragments arranged by size:
1180 495 35
DraIII CAC_nnn'GTG
Cuts at: 0 24 138 303 1008 1011 1710
Size: 24 114 165 705 3 699
Fragments arranged by size:
705 699 165 114 24 3
DrdI GACnn_nn'nnGTC
Cuts at: 0 1692 1710
Size: 1692 18
EaeI y'GGCC_r
Cuts at: 0 332 786 876 1079 1433 1574 1646 1655
Size: 332 454 90 203 354 141 72 9
Cuts at: 1655 1710
Size: 55
Fragments arranged by size:
454 354 332 203 141 90 72 55
9
EarI CTCTTCn'nnn_
Cuts at: 0 631 1229 1553 1710
Size: 631 598 324 157
Fragments arranged by size:
631 598 324 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1178 1710
Size: 1178 532
EcoICRI GAG'CTC
Cuts at: 0 562 1710
Size: 562 1148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 433 655 1132 1295 1630 1702 1710
Size: 372 61 222 477 163 335 72 8
Fragments arranged by size:
477 372 335 222 163 72 61 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1222 1710
Size: 1222 488
EcoO109I rG'GnC_Cy
Cuts at: 0 360 848 1137 1286 1287 1356 1710
Size: 360 488 289 149 1 69 354
Fragments arranged by size:
488 360 354 289 149 69 1
FspI TGC'GCA
Cuts at: 0 829 919 1710
Size: 829 90 791
Fragments arranged by size:
829 791 90
FspAI rTGC'GCAy
Cuts at: 0 829 919 1710
Size: 829 90 791
Fragments arranged by size:
829 791 90
HaeII r_GCGC'y
Cuts at: 0 896 1472 1710
Size: 896 576 238
Fragments arranged by size:
896 576 238
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1232 1264 1710
Size: 1232 32 446
Fragments arranged by size:
1232 446 32
HindIII A'AGCT_T
Cuts at: 0 79 1710
Size: 79 1631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 459 1676 1710
Size: 288 171 1217 34
Fragments arranged by size:
1217 288 171 34
MscI TGG'CCA
Cuts at: 0 1081 1435 1576 1648 1710
Size: 1081 354 141 72 62
Fragments arranged by size:
1081 354 141 72 62
MslI CAynn'nnrTG
Cuts at: 0 569 585 659 675 749 1375 1600 1642
Size: 569 16 74 16 74 626 225 42
Cuts at: 1642 1710
Size: 68
Fragments arranged by size:
626 569 225 74 74 68 42 16
16
MspA1I CmG'CkG
Cuts at: 0 189 497 602 881 1608 1710
Size: 189 308 105 279 727 102
Fragments arranged by size:
727 308 279 189 105 102
NcoI C'CATG_G
Cuts at: 0 1101 1460 1710
Size: 1101 359 250
Fragments arranged by size:
1101 359 250
NspI r_CATG'y
Cuts at: 0 736 826 1710
Size: 736 90 884
Fragments arranged by size:
884 736 90
PasI CC'CwG_GG
Cuts at: 0 1359 1710
Size: 1359 351
PciI A'CATG_T
Cuts at: 0 822 1710
Size: 822 888
PflMI CCAn_nnn'nTGG
Cuts at: 0 303 1710
Size: 303 1407
PmlI CAC'GTG
Cuts at: 0 915 1008 1710
Size: 915 93 702
Fragments arranged by size:
915 702 93
PpuMI rG'GwC_Cy
Cuts at: 0 360 1710
Size: 360 1350
PspOMI G'GGCC_C
Cuts at: 0 1286 1710
Size: 1286 424
PspXI vC'TCGA_Gb
Cuts at: 0 106 987 1710
Size: 106 881 723
Fragments arranged by size:
881 723 106
PstI C_TGCA'G
Cuts at: 0 728 1042 1176 1355 1710
Size: 728 314 134 179 355
Fragments arranged by size:
728 355 314 179 134
PvuII CAG'CTG
Cuts at: 0 189 602 1608 1710
Size: 189 413 1006 102
Fragments arranged by size:
1006 413 189 102
SacI G_AGCT'C
Cuts at: 0 564 1710
Size: 564 1146
SacII CC_GC'GG
Cuts at: 0 882 1710
Size: 882 828
SapI GCTCTTCn'nnn_
Cuts at: 0 631 1710
Size: 631 1079
SbfI CC_TGCA'GG
Cuts at: 0 1355 1710
Size: 1355 355
ScaI AGT'ACT
Cuts at: 0 985 1710
Size: 985 725
SexAI A'CCwGG_T
Cuts at: 0 134 1710
Size: 134 1576
SfcI C'TryA_G
Cuts at: 0 724 1038 1172 1351 1710
Size: 724 314 134 179 359
Fragments arranged by size:
724 359 314 179 134
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1654 1710
Size: 1654 56
SmlI C'TyrA_G
Cuts at: 0 106 987 1159 1533 1710
Size: 106 881 172 374 177
Fragments arranged by size:
881 374 177 172 106
StuI AGG'CCT
Cuts at: 0 613 703 1710
Size: 613 90 1007
Fragments arranged by size:
1007 613 90
StyI C'CwwG_G
Cuts at: 0 1076 1101 1460 1710
Size: 1076 25 359 250
Fragments arranged by size:
1076 359 250 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 240 847 1209 1399 1495 1710
Size: 240 607 362 190 96 215
Fragments arranged by size:
607 362 240 215 190 96
TatI w'GTAC_w
Cuts at: 0 983 1710
Size: 983 727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 109 271 1710
Size: 109 162 1439
Fragments arranged by size:
1439 162 109
XhoI C'TCGA_G
Cuts at: 0 106 987 1710
Size: 106 881 723
Fragments arranged by size:
881 723 106
XmnI GAAnn'nnTTC
Cuts at: 0 973 1710
Size: 973 737
Enzymes that do cut:
AarI AcuI AflIII AleI AloI AlwNI ApaI ApaLI
AvaI BaeI BanI BanII BbsI BbvCI BclI BglI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI
HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI
NspI PasI PciI PflMI PmlI PpuMI PspOMI PspXI
PstI PvuII SacI SacII SapI SbfI ScaI SexAI
SfcI SfiI SmlI StuI StyI TaqII TatI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApoI AscI AseI AsiSI AvrII BamHI BbeI
BcgI BciVI BfrBI BglII BlpI BmgBI BmtI BsaBI
BsaWI BsaXI BsiEI BsiWI BspEI BsrGI BssHII BssSI
BstBI BstYI BstZ17I ClaI DraI EagI EcoRI EcoRV
FalI FseI HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PfoI PmeI PpiI PshAI PsiI PsrI PvuI
RsrII SalI SanDI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XmaI ZraI