Restriction map for
pF1KB7886
(Linear) (Six-base) MAPSORT of: KIBB7886.seq Check: 8932 from: 1 to: 1953
KIBB7886.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 818 1441 1525 1720 1778 1953
Size: 202 616 623 84 195 58 175
Fragments arranged by size:
623 616 202 195 175 84 58
AflIII A'CryG_T
Cuts at: 0 22 553 1953
Size: 22 531 1400
Fragments arranged by size:
1400 531 22
AlwNI CAG_nnn'CTG
Cuts at: 0 514 1464 1608 1953
Size: 514 950 144 345
Fragments arranged by size:
950 514 345 144
ApoI r'AATT_y
Cuts at: 0 280 543 631 725 1243 1467 1714 1953
Size: 280 263 88 94 518 224 247 239
Fragments arranged by size:
518 280 263 247 239 224 94 88
AvaI C'yCGr_G
Cuts at: 0 473 1953
Size: 473 1480
BanI G'GyrC_C
Cuts at: 0 6 1953
Size: 6 1947
BanII G_rGCy'C
Cuts at: 0 1918 1953
Size: 1918 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 1560 1751 1953
Size: 1560 191 202
Fragments arranged by size:
1560 202 191
BclI T'GATC_A
Cuts at: 0 479 1953
Size: 479 1474
BfrBI ATG'CAT
Cuts at: 0 772 1953
Size: 772 1181
BglII A'GATC_T
Cuts at: 0 1278 1649 1953
Size: 1278 371 304
Fragments arranged by size:
1278 371 304
Bme1580I G_kGCm'C
Cuts at: 0 1841 1953
Size: 1841 112
BmrI ACTGGGnnnn_n'
Cuts at: 0 95 1937 1953
Size: 95 1842 16
Fragments arranged by size:
1842 95 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1652 1953
Size: 751 901 301
Fragments arranged by size:
901 751 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 739 1953
Size: 735 4 1214
Fragments arranged by size:
1214 735 4
BsaBI GATnn'nnATC
Cuts at: 0 530 1028 1045 1861 1953
Size: 530 498 17 816 92
Fragments arranged by size:
816 530 498 92 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1628 1658 1953
Size: 1628 30 295
Fragments arranged by size:
1628 295 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1649 1953
Size: 1649 304
BseYI C'CCAG_C
Cuts at: 0 69 1953
Size: 69 1884
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1282 1638 1666 1953
Size: 1282 356 28 287
Fragments arranged by size:
1282 356 287 28
BsiEI CG_ry'CG
Cuts at: 0 44 1953
Size: 44 1909
BsmI GAATG_Cn'
Cuts at: 0 774 1336 1953
Size: 774 562 617
Fragments arranged by size:
774 617 562
Bsp1286I G_dGCh'C
Cuts at: 0 1841 1918 1953
Size: 1841 77 35
Fragments arranged by size:
1841 77 35
BsrBI CCG'CTC
Cuts at: 0 1427 1476 1953
Size: 1427 49 477
Fragments arranged by size:
1427 477 49
BsrGI T'GTAC_A
Cuts at: 0 1089 1931 1953
Size: 1089 842 22
Fragments arranged by size:
1089 842 22
BstBI TT'CG_AA
Cuts at: 0 645 1953
Size: 645 1308
BstYI r'GATC_y
Cuts at: 0 237 1023 1278 1649 1953
Size: 237 786 255 371 304
Fragments arranged by size:
786 371 304 255 237
BtgI C'CryG_G
Cuts at: 0 601 836 1356 1953
Size: 601 235 520 597
Fragments arranged by size:
601 597 520 235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 509 1358 1953
Size: 509 849 595
Fragments arranged by size:
849 595 509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 605 640 1892 1910 1927 1945 1953
Size: 605 35 1252 18 17 18 8
Fragments arranged by size:
1252 605 35 18 18 17 8
EaeI y'GGCC_r
Cuts at: 0 41 1953
Size: 41 1912
EagI C'GGCC_G
Cuts at: 0 41 1953
Size: 41 1912
EarI CTCTTCn'nnn_
Cuts at: 0 374 1696 1754 1953
Size: 374 1322 58 199
Fragments arranged by size:
1322 374 199 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 751 818 1441 1525 1652 1720 1778
Size: 202 549 67 623 84 127 68 58
Cuts at: 1778 1953
Size: 175
Fragments arranged by size:
623 549 202 175 127 84 68 67
58
EcoO109I rG'GnC_Cy
Cuts at: 0 1173 1953
Size: 1173 780
EcoRI G'AATT_C
Cuts at: 0 280 1953
Size: 280 1673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 365 1953
Size: 365 1588
HaeII r_GCGC'y
Cuts at: 0 939 1953
Size: 939 1014
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 425 457 906 938 1581 1613 1953
Size: 425 32 449 32 643 32 340
Fragments arranged by size:
643 449 425 340 32 32 32
HincII GTy'rAC
Cuts at: 0 1290 1530 1953
Size: 1290 240 423
Fragments arranged by size:
1290 423 240
HindIII A'AGCT_T
Cuts at: 0 1224 1953
Size: 1224 729
HpaI GTT'AAC
Cuts at: 0 1290 1530 1953
Size: 1290 240 423
Fragments arranged by size:
1290 423 240
MluI A'CGCG_T
Cuts at: 0 22 1953
Size: 22 1931
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 740 1953
Size: 733 7 1213
Fragments arranged by size:
1213 733 7
MslI CAynn'nnrTG
Cuts at: 0 666 949 1953
Size: 666 283 1004
Fragments arranged by size:
1004 666 283
MspA1I CmG'CkG
Cuts at: 0 231 1630 1665 1953
Size: 231 1399 35 288
Fragments arranged by size:
1399 288 231 35
NcoI C'CATG_G
Cuts at: 0 1356 1953
Size: 1356 597
NotI GC'GGCC_GC
Cuts at: 0 41 1953
Size: 41 1912
NruI TCG'CGA
Cuts at: 0 1034 1953
Size: 1034 919
NsiI A_TGCA'T
Cuts at: 0 774 1953
Size: 774 1179
NspI r_CATG'y
Cuts at: 0 557 1938 1953
Size: 557 1381 15
Fragments arranged by size:
1381 557 15
PciI A'CATG_T
Cuts at: 0 553 1953
Size: 553 1400
PpuMI rG'GwC_Cy
Cuts at: 0 1173 1953
Size: 1173 780
PshAI GACnn'nnGTC
Cuts at: 0 172 554 1953
Size: 172 382 1399
Fragments arranged by size:
1399 382 172
PstI C_TGCA'G
Cuts at: 0 60 1953
Size: 60 1893
PvuII CAG'CTG
Cuts at: 0 231 1953
Size: 231 1722
SapI GCTCTTCn'nnn_
Cuts at: 0 1754 1953
Size: 1754 199
SfcI C'TryA_G
Cuts at: 0 56 926 1113 1765 1953
Size: 56 870 187 652 188
Fragments arranged by size:
870 652 188 187 56
SmlI C'TyrA_G
Cuts at: 0 714 754 1953
Size: 714 40 1199
Fragments arranged by size:
1199 714 40
StyI C'CwwG_G
Cuts at: 0 349 1356 1953
Size: 349 1007 597
Fragments arranged by size:
1007 597 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1087 1953
Size: 1087 866
TatI w'GTAC_w
Cuts at: 0 185 299 1089 1931 1953
Size: 185 114 790 842 22
Fragments arranged by size:
842 790 185 114 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1628 1660 1953
Size: 1628 32 293
Fragments arranged by size:
1628 293 32
Tth111I GACn'n_nGTC
Cuts at: 0 437 1953
Size: 437 1516
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 599 1953
Size: 599 1354
XmnI GAAnn'nnTTC
Cuts at: 0 632 1899 1953
Size: 632 1267 54
Fragments arranged by size:
1267 632 54
Enzymes that do cut:
AcuI AflIII AlwNI ApoI AvaI BanI BanII BbsI
BclI BfrBI BglII Bme1580I BmrI BpmI BpuEI BsaBI
BsaXI BseRI BseYI BsgI BsiEI BsmI Bsp1286I BsrBI
BsrGI BstBI BstYI BtgI BtgZI CspCI EaeI EagI
EarI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII HpaI MluI MmeI MslI MspA1I NcoI NotI
NruI NsiI NspI PciI PpuMI PshAI PstI PvuII
SapI SfcI SmlI StyI TaqII TatI TstI Tth111I
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BglI BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI
BsaHI BsaWI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI
BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI DraI DraIII DrdI EciI EcoICRI
EcoNI EcoRV FseI FspI FspAI KasI KpnI MfeI
MscI NaeI NarI NdeI NgoMIV NheI PacI PasI
PflMI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI XbaI XhoI
XmaI ZraI