Restriction map for
pF1KB7888
(Linear) (Six-base) MAPSORT of: KIBB7888.seq Check: 2658 from: 1 to: 1953
KIBB7888.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 0 1947 1953
Size: 1947 6
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 731 1635 1953
Size: 129 602 904 318
Fragments arranged by size:
904 602 318 129
AlwNI CAG_nnn'CTG
Cuts at: 0 117 1953
Size: 117 1836
ApaLI G'TGCA_C
Cuts at: 0 1190 1953
Size: 1190 763
ApoI r'AATT_y
Cuts at: 0 259 367 605 737 917 1066 1899 1953
Size: 259 108 238 132 180 149 833 54
Fragments arranged by size:
833 259 238 180 149 132 108 54
AseI AT'TA_AT
Cuts at: 0 1253 1953
Size: 1253 700
AvaI C'yCGr_G
Cuts at: 0 935 1953
Size: 935 1018
BamHI G'GATC_C
Cuts at: 0 1368 1953
Size: 1368 585
BanI G'GyrC_C
Cuts at: 0 76 214 1953
Size: 76 138 1739
Fragments arranged by size:
1739 138 76
BanII G_rGCy'C
Cuts at: 0 163 1953
Size: 163 1790
BbeI G_GCGC'C
Cuts at: 0 218 1953
Size: 218 1735
BfrBI ATG'CAT
Cuts at: 0 1696 1953
Size: 1696 257
BglI GCCn_nnn'nGGC
Cuts at: 0 74 1953
Size: 74 1879
BglII A'GATC_T
Cuts at: 0 615 684 1953
Size: 615 69 1269
Fragments arranged by size:
1269 615 69
BlpI GC'TnA_GC
Cuts at: 0 1350 1953
Size: 1350 603
Bme1580I G_kGCm'C
Cuts at: 0 315 1194 1953
Size: 315 879 759
Fragments arranged by size:
879 759 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 779 1953
Size: 482 297 1174
Fragments arranged by size:
1174 482 297
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1179 1214 1953
Size: 1179 35 739
Fragments arranged by size:
1179 739 35
BsaBI GATnn'nnATC
Cuts at: 0 28 689 1953
Size: 28 661 1264
Fragments arranged by size:
1264 661 28
BsaHI Gr'CG_yC
Cuts at: 0 215 1953
Size: 215 1738
BsaWI w'CCGG_w
Cuts at: 0 1089 1953
Size: 1089 864
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 184 187 1953
Size: 181 3 3 1766
Fragments arranged by size:
1766 181 3 3
BseYI C'CCAG_C
Cuts at: 0 131 1953
Size: 131 1822
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1916 1953
Size: 653 1263 37
Fragments arranged by size:
1263 653 37
BsiHKAI G_wGCw'C
Cuts at: 0 1194 1953
Size: 1194 759
BsmBI CGTCTCn'nnnn_
Cuts at: 0 76 1953
Size: 76 1877
Bsp1286I G_dGCh'C
Cuts at: 0 163 315 1194 1953
Size: 163 152 879 759
Fragments arranged by size:
879 759 163 152
BspEI T'CCGG_A
Cuts at: 0 1089 1953
Size: 1089 864
BspHI T'CATG_A
Cuts at: 0 1169 1953
Size: 1169 784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 42 98 1953
Size: 42 56 1855
Fragments arranged by size:
1855 56 42
BsrDI GCAATG_nn'
Cuts at: 0 1090 1953
Size: 1090 863
BsrGI T'GTAC_A
Cuts at: 0 1512 1953
Size: 1512 441
BssHII G'CGCG_C
Cuts at: 0 12 1953
Size: 12 1941
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1122 1953
Size: 1122 831
BstBI TT'CG_AA
Cuts at: 0 588 1953
Size: 588 1365
BstEII G'GTnAC_C
Cuts at: 0 805 1953
Size: 805 1148
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1629 1710 1953
Size: 1629 81 243
Fragments arranged by size:
1629 243 81
BstYI r'GATC_y
Cuts at: 0 615 637 684 1368 1953
Size: 615 22 47 684 585
Fragments arranged by size:
684 615 585 47 22
BtsI GCAGTG_nn'
Cuts at: 0 23 1953
Size: 23 1930
DraIII CAC_nnn'GTG
Cuts at: 0 975 1953
Size: 975 978
DrdI GACnn_nn'nnGTC
Cuts at: 0 1223 1953
Size: 1223 730
EaeI y'GGCC_r
Cuts at: 0 208 1200 1953
Size: 208 992 753
Fragments arranged by size:
992 753 208
EarI CTCTTCn'nnn_
Cuts at: 0 832 1521 1953
Size: 832 689 432
Fragments arranged by size:
832 689 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 482 731 779 1635 1953
Size: 129 353 249 48 856 318
Fragments arranged by size:
856 353 318 249 129 48
EcoO109I rG'GnC_Cy
Cuts at: 0 1225 1953
Size: 1225 728
EcoRV GAT'ATC
Cuts at: 0 440 1953
Size: 440 1513
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1211 1953
Size: 1211 742
HaeII r_GCGC'y
Cuts at: 0 218 1953
Size: 218 1735
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 366 373 398 405 981 1013 1042 1074
Size: 366 7 25 7 576 32 29 32
Cuts at: 1074 1164 1196 1368 1400 1953
Size: 90 32 172 32 553
Fragments arranged by size:
576 553 366 172 90 32 32 32
32 29 25 7 7
HincII GTy'rAC
Cuts at: 0 822 1953
Size: 822 1131
HindIII A'AGCT_T
Cuts at: 0 1232 1953
Size: 1232 721
HpaI GTT'AAC
Cuts at: 0 822 1953
Size: 822 1131
KasI G'GCGC_C
Cuts at: 0 214 1953
Size: 214 1739
MfeI C'AATT_G
Cuts at: 0 433 1480 1953
Size: 433 1047 473
Fragments arranged by size:
1047 473 433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 1953
Size: 576 1377
MscI TGG'CCA
Cuts at: 0 1202 1953
Size: 1202 751
MslI CAynn'nnrTG
Cuts at: 0 976 1103 1171 1520 1708 1853 1953
Size: 976 127 68 349 188 145 100
Fragments arranged by size:
976 349 188 145 127 100 68
MspA1I CmG'CkG
Cuts at: 0 99 135 1953
Size: 99 36 1818
Fragments arranged by size:
1818 99 36
NarI GG'CG_CC
Cuts at: 0 215 1953
Size: 215 1738
NsiI A_TGCA'T
Cuts at: 0 1698 1953
Size: 1698 255
NspI r_CATG'y
Cuts at: 0 1606 1953
Size: 1606 347
PflMI CCAn_nnn'nTGG
Cuts at: 0 63 1953
Size: 63 1890
PpuMI rG'GwC_Cy
Cuts at: 0 1225 1953
Size: 1225 728
PsiI TTA'TAA
Cuts at: 0 1910 1953
Size: 1910 43
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1592 1624 1953
Size: 1592 32 329
Fragments arranged by size:
1592 329 32
ScaI AGT'ACT
Cuts at: 0 1643 1953
Size: 1643 310
SexAI A'CCwGG_T
Cuts at: 0 855 1595 1953
Size: 855 740 358
Fragments arranged by size:
855 740 358
SfcI C'TryA_G
Cuts at: 0 624 1561 1676 1953
Size: 624 937 115 277
Fragments arranged by size:
937 624 277 115
SfoI GGC'GCC
Cuts at: 0 216 1953
Size: 216 1737
SmlI C'TyrA_G
Cuts at: 0 1158 1229 1953
Size: 1158 71 724
Fragments arranged by size:
1158 724 71
SspI AAT'ATT
Cuts at: 0 1488 1953
Size: 1488 465
StyI C'CwwG_G
Cuts at: 0 280 1622 1953
Size: 280 1342 331
Fragments arranged by size:
1342 331 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 202 1953
Size: 202 1751
TatI w'GTAC_w
Cuts at: 0 1512 1641 1953
Size: 1512 129 312
Fragments arranged by size:
1512 312 129
XmnI GAAnn'nnTTC
Cuts at: 0 944 1277 1953
Size: 944 333 676
Fragments arranged by size:
944 676 333
Enzymes that do cut:
AccI AcuI AlwNI ApaLI ApoI AseI AvaI BamHI
BanI BanII BbeI BfrBI BglI BglII BlpI Bme1580I
BpmI BpuEI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstYI BtsI DraIII
DrdI EaeI EarI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MmeI MscI
MslI MspA1I NarI NsiI NspI PflMI PpuMI PsiI
PsrI ScaI SexAI SfcI SfoI SmlI SspI StyI
TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AsiSI AvrII
BaeI BbsI BbvCI BcgI BciVI BclI BmgBI BmrI
BmtI BplI Bpu10I BsaI BsaAI BsaXI BsiEI BsiWI
BsmI BsrBI BsrFI BssSI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI EagI EciI EcoICRI EcoNI EcoRI
FseI FspI FspAI KpnI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI