(Linear) (Six-base) MAPSORT of: KIBB7904.seq Check: 3182 from: 1 to: 984 KIBB7904.orf With 182 enzymes: SgfI * October 7, 2009 14:24 .. AccI GT'mk_AC Cuts at: 0 597 984 Size: 597 387 AclI AA'CG_TT Cuts at: 0 19 984 Size: 19 965 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 254 293 768 984 Size: 254 39 475 216 Fragments arranged by size: 475 254 216 39 AflIII A'CryG_T Cuts at: 0 332 984 Size: 332 652 ApoI r'AATT_y Cuts at: 0 583 628 984 Size: 583 45 356 Fragments arranged by size: 583 356 45 AseI AT'TA_AT Cuts at: 0 212 984 Size: 212 772 BbsI GAAGACnn'nnnn_ Cuts at: 0 324 401 984 Size: 324 77 583 Fragments arranged by size: 583 324 77 BglII A'GATC_T Cuts at: 0 276 984 Size: 276 708 Bpu10I CC'TnA_GC Cuts at: 0 443 984 Size: 443 541 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 245 275 984 Size: 245 30 709 Fragments arranged by size: 709 245 30 BsiHKAI G_wGCw'C Cuts at: 0 838 984 Size: 838 146 Bsp1286I G_dGCh'C Cuts at: 0 838 984 Size: 838 146 BspHI T'CATG_A Cuts at: 0 165 984 Size: 165 819 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 122 984 Size: 122 862 BsrDI GCAATG_nn' Cuts at: 0 494 689 984 Size: 494 195 295 Fragments arranged by size: 494 295 195 BstEII G'GTnAC_C Cuts at: 0 762 984 Size: 762 222 BstXI CCAn_nnnn'nTGG Cuts at: 0 922 984 Size: 922 62 BstYI r'GATC_y Cuts at: 0 276 732 984 Size: 276 456 252 Fragments arranged by size: 456 276 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 881 984 Size: 881 103 BtsI GCAGTG_nn' Cuts at: 0 112 836 984 Size: 112 724 148 Fragments arranged by size: 724 148 112 ClaI AT'CG_AT Cuts at: 0 374 984 Size: 374 610 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 387 422 984 Size: 387 35 562 Fragments arranged by size: 562 387 35 EarI CTCTTCn'nnn_ Cuts at: 0 167 746 984 Size: 167 579 238 Fragments arranged by size: 579 238 167 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 254 293 768 984 Size: 254 39 475 216 Fragments arranged by size: 475 254 216 39 EcoNI CCTnn'n_nnAGG Cuts at: 0 954 984 Size: 954 30 EcoO109I rG'GnC_Cy Cuts at: 0 777 984 Size: 777 207 EcoRI G'AATT_C Cuts at: 0 583 984 Size: 583 401 FspI TGC'GCA Cuts at: 0 295 984 Size: 295 689 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 337 369 984 Size: 337 32 615 Fragments arranged by size: 615 337 32 HincII GTy'rAC Cuts at: 0 598 984 Size: 598 386 HindIII A'AGCT_T Cuts at: 0 620 984 Size: 620 364 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 623 984 Size: 623 361 MslI CAynn'nnrTG Cuts at: 0 866 984 Size: 866 118 MspA1I CmG'CkG Cuts at: 0 246 984 Size: 246 738 NspI r_CATG'y Cuts at: 0 336 984 Size: 336 648 PacI TTA_AT'TAA Cuts at: 0 212 984 Size: 212 772 PciI A'CATG_T Cuts at: 0 332 984 Size: 332 652 PpuMI rG'GwC_Cy Cuts at: 0 777 984 Size: 777 207 PstI C_TGCA'G Cuts at: 0 652 843 984 Size: 652 191 141 Fragments arranged by size: 652 191 141 PvuII CAG'CTG Cuts at: 0 246 984 Size: 246 738 SalI G'TCGA_C Cuts at: 0 596 984 Size: 596 388 SanDI GG'GwC_CC Cuts at: 0 777 984 Size: 777 207 SapI GCTCTTCn'nnn_ Cuts at: 0 746 984 Size: 746 238 SfcI C'TryA_G Cuts at: 0 59 648 839 909 984 Size: 59 589 191 70 75 Fragments arranged by size: 589 191 75 70 59 StuI AGG'CCT Cuts at: 0 159 984 Size: 159 825 StyI C'CwwG_G Cuts at: 0 767 984 Size: 767 217 XmnI GAAnn'nnTTC Cuts at: 0 278 984 Size: 278 706 Enzymes that do cut: AccI AclI AcuI AflIII ApoI AseI BbsI BglII Bpu10I BsaXI BsiHKAI Bsp1286I BspHI BspMI BsrDI BstEII BstXI BstYI BtgZI BtsI ClaI CspCI EarI Eco57MI EcoNI EcoO109I EcoRI FspI Hin4I HincII HindIII MmeI MslI MspA1I NspI PacI PciI PpuMI PstI PvuII SalI SanDI SapI SfcI StuI StyI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoRV FalI FseI FspAI HaeII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PasI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI