Restriction map for pF1KB7904
 (Linear) (Six-base) MAPSORT of: KIBB7904.seq  Check: 3182  from: 1  to: 984



KIBB7904.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AccI GT'mk_AC

Cuts at:      0     597     984 
   Size:        597     387
 
AclI AA'CG_TT

Cuts at:      0      19     984 
   Size:         19     965
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     293     768     984 
   Size:        254      39     475     216

  Fragments arranged by size: 

                 475     254     216      39
 
AflIII A'CryG_T

Cuts at:      0     332     984 
   Size:        332     652
 
ApoI r'AATT_y

Cuts at:      0     583     628     984 
   Size:        583      45     356

  Fragments arranged by size: 

                 583     356      45
 
AseI AT'TA_AT

Cuts at:      0     212     984 
   Size:        212     772
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     324     401     984 
   Size:        324      77     583

  Fragments arranged by size: 

                 583     324      77
 
BglII A'GATC_T

Cuts at:      0     276     984 
   Size:        276     708
 
Bpu10I CC'TnA_GC

Cuts at:      0     443     984 
   Size:        443     541
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     245     275     984 
   Size:        245      30     709

  Fragments arranged by size: 

                 709     245      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     838     984 
   Size:        838     146
 
Bsp1286I G_dGCh'C

Cuts at:      0     838     984 
   Size:        838     146
 
BspHI T'CATG_A

Cuts at:      0     165     984 
   Size:        165     819
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     122     984 
   Size:        122     862
 
BsrDI GCAATG_nn'

Cuts at:      0     494     689     984 
   Size:        494     195     295

  Fragments arranged by size: 

                 494     295     195
 
BstEII G'GTnAC_C

Cuts at:      0     762     984 
   Size:        762     222
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     922     984 
   Size:        922      62
 
BstYI r'GATC_y

Cuts at:      0     276     732     984 
   Size:        276     456     252

  Fragments arranged by size: 

                 456     276     252
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     881     984 
   Size:        881     103
 
BtsI GCAGTG_nn'

Cuts at:      0     112     836     984 
   Size:        112     724     148

  Fragments arranged by size: 

                 724     148     112
 
ClaI AT'CG_AT

Cuts at:      0     374     984 
   Size:        374     610
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     387     422     984 
   Size:        387      35     562

  Fragments arranged by size: 

                 562     387      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     167     746     984 
   Size:        167     579     238

  Fragments arranged by size: 

                 579     238     167
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     293     768     984 
   Size:        254      39     475     216

  Fragments arranged by size: 

                 475     254     216      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     954     984 
   Size:        954      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0     777     984 
   Size:        777     207
 
EcoRI G'AATT_C

Cuts at:      0     583     984 
   Size:        583     401
 
FspI TGC'GCA

Cuts at:      0     295     984 
   Size:        295     689
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     337     369     984 
   Size:        337      32     615

  Fragments arranged by size: 

                 615     337      32
 
HincII GTy'rAC

Cuts at:      0     598     984 
   Size:        598     386
 
HindIII A'AGCT_T

Cuts at:      0     620     984 
   Size:        620     364
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     623     984 
   Size:        623     361
 
MslI CAynn'nnrTG

Cuts at:      0     866     984 
   Size:        866     118
 
MspA1I CmG'CkG

Cuts at:      0     246     984 
   Size:        246     738
 
NspI r_CATG'y

Cuts at:      0     336     984 
   Size:        336     648
 
PacI TTA_AT'TAA

Cuts at:      0     212     984 
   Size:        212     772
 
PciI A'CATG_T

Cuts at:      0     332     984 
   Size:        332     652
 
PpuMI rG'GwC_Cy

Cuts at:      0     777     984 
   Size:        777     207
 
PstI C_TGCA'G

Cuts at:      0     652     843     984 
   Size:        652     191     141

  Fragments arranged by size: 

                 652     191     141
 
PvuII CAG'CTG

Cuts at:      0     246     984 
   Size:        246     738
 
SalI G'TCGA_C

Cuts at:      0     596     984 
   Size:        596     388
 
SanDI GG'GwC_CC

Cuts at:      0     777     984 
   Size:        777     207
 
SapI GCTCTTCn'nnn_

Cuts at:      0     746     984 
   Size:        746     238
 
SfcI C'TryA_G

Cuts at:      0      59     648     839     909     984 
   Size:         59     589     191      70      75

  Fragments arranged by size: 

                 589     191      75      70      59
 
StuI AGG'CCT

Cuts at:      0     159     984 
   Size:        159     825
 
StyI C'CwwG_G

Cuts at:      0     767     984 
   Size:        767     217
 
XmnI GAAnn'nnTTC

Cuts at:      0     278     984 
   Size:        278     706
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     ApoI     AseI     BbsI    BglII
   Bpu10I    BsaXI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI   BstEII
    BstXI    BstYI    BtgZI     BtsI     ClaI    CspCI     EarI  Eco57MI
    EcoNI EcoO109I    EcoRI     FspI    Hin4I   HincII  HindIII     MmeI
     MslI   MspA1I     NspI     PacI     PciI    PpuMI     PstI    PvuII
     SalI    SanDI     SapI     SfcI     StuI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
  BstZ17I   Bsu36I     BtgI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoRV     FalI     FseI    FspAI    HaeII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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