Restriction map for
pF1KB7904
(Linear) (Six-base) MAPSORT of: KIBB7904.seq Check: 3182 from: 1 to: 984
KIBB7904.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 0 597 984
Size: 597 387
AclI AA'CG_TT
Cuts at: 0 19 984
Size: 19 965
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 293 768 984
Size: 254 39 475 216
Fragments arranged by size:
475 254 216 39
AflIII A'CryG_T
Cuts at: 0 332 984
Size: 332 652
ApoI r'AATT_y
Cuts at: 0 583 628 984
Size: 583 45 356
Fragments arranged by size:
583 356 45
AseI AT'TA_AT
Cuts at: 0 212 984
Size: 212 772
BbsI GAAGACnn'nnnn_
Cuts at: 0 324 401 984
Size: 324 77 583
Fragments arranged by size:
583 324 77
BglII A'GATC_T
Cuts at: 0 276 984
Size: 276 708
Bpu10I CC'TnA_GC
Cuts at: 0 443 984
Size: 443 541
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 245 275 984
Size: 245 30 709
Fragments arranged by size:
709 245 30
BsiHKAI G_wGCw'C
Cuts at: 0 838 984
Size: 838 146
Bsp1286I G_dGCh'C
Cuts at: 0 838 984
Size: 838 146
BspHI T'CATG_A
Cuts at: 0 165 984
Size: 165 819
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 122 984
Size: 122 862
BsrDI GCAATG_nn'
Cuts at: 0 494 689 984
Size: 494 195 295
Fragments arranged by size:
494 295 195
BstEII G'GTnAC_C
Cuts at: 0 762 984
Size: 762 222
BstXI CCAn_nnnn'nTGG
Cuts at: 0 922 984
Size: 922 62
BstYI r'GATC_y
Cuts at: 0 276 732 984
Size: 276 456 252
Fragments arranged by size:
456 276 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 881 984
Size: 881 103
BtsI GCAGTG_nn'
Cuts at: 0 112 836 984
Size: 112 724 148
Fragments arranged by size:
724 148 112
ClaI AT'CG_AT
Cuts at: 0 374 984
Size: 374 610
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 387 422 984
Size: 387 35 562
Fragments arranged by size:
562 387 35
EarI CTCTTCn'nnn_
Cuts at: 0 167 746 984
Size: 167 579 238
Fragments arranged by size:
579 238 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 293 768 984
Size: 254 39 475 216
Fragments arranged by size:
475 254 216 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 954 984
Size: 954 30
EcoO109I rG'GnC_Cy
Cuts at: 0 777 984
Size: 777 207
EcoRI G'AATT_C
Cuts at: 0 583 984
Size: 583 401
FspI TGC'GCA
Cuts at: 0 295 984
Size: 295 689
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 337 369 984
Size: 337 32 615
Fragments arranged by size:
615 337 32
HincII GTy'rAC
Cuts at: 0 598 984
Size: 598 386
HindIII A'AGCT_T
Cuts at: 0 620 984
Size: 620 364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 984
Size: 623 361
MslI CAynn'nnrTG
Cuts at: 0 866 984
Size: 866 118
MspA1I CmG'CkG
Cuts at: 0 246 984
Size: 246 738
NspI r_CATG'y
Cuts at: 0 336 984
Size: 336 648
PacI TTA_AT'TAA
Cuts at: 0 212 984
Size: 212 772
PciI A'CATG_T
Cuts at: 0 332 984
Size: 332 652
PpuMI rG'GwC_Cy
Cuts at: 0 777 984
Size: 777 207
PstI C_TGCA'G
Cuts at: 0 652 843 984
Size: 652 191 141
Fragments arranged by size:
652 191 141
PvuII CAG'CTG
Cuts at: 0 246 984
Size: 246 738
SalI G'TCGA_C
Cuts at: 0 596 984
Size: 596 388
SanDI GG'GwC_CC
Cuts at: 0 777 984
Size: 777 207
SapI GCTCTTCn'nnn_
Cuts at: 0 746 984
Size: 746 238
SfcI C'TryA_G
Cuts at: 0 59 648 839 909 984
Size: 59 589 191 70 75
Fragments arranged by size:
589 191 75 70 59
StuI AGG'CCT
Cuts at: 0 159 984
Size: 159 825
StyI C'CwwG_G
Cuts at: 0 767 984
Size: 767 217
XmnI GAAnn'nnTTC
Cuts at: 0 278 984
Size: 278 706
Enzymes that do cut:
AccI AclI AcuI AflIII ApoI AseI BbsI BglII
Bpu10I BsaXI BsiHKAI Bsp1286I BspHI BspMI BsrDI BstEII
BstXI BstYI BtgZI BtsI ClaI CspCI EarI Eco57MI
EcoNI EcoO109I EcoRI FspI Hin4I HincII HindIII MmeI
MslI MspA1I NspI PacI PciI PpuMI PstI PvuII
SalI SanDI SapI SfcI StuI StyI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoRV FalI FseI FspAI HaeII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PasI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI