(Linear) (Six-base) MAPSORT of: KIBB7905.seq Check: 5119 from: 1 to: 372
KIBB7905.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
BbsI GAAGACnn'nnnn_
Cuts at: 0 23 234 252 372
Size: 23 211 18 120
Fragments arranged by size:
211 120 23 18
BglII A'GATC_T
Cuts at: 0 174 372
Size: 174 198
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 68 372
Size: 68 304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 372
Size: 80 292
BsrDI GCAATG_nn'
Cuts at: 0 221 372
Size: 221 151
BstBI TT'CG_AA
Cuts at: 0 270 372
Size: 270 102
BstYI r'GATC_y
Cuts at: 0 174 367 372
Size: 174 193 5
Fragments arranged by size:
193 174 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 123 372
Size: 123 249
EcoO109I rG'GnC_Cy
Cuts at: 0 276 372
Size: 276 96
EcoRV GAT'ATC
Cuts at: 0 255 372
Size: 255 117
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 325 372
Size: 325 47
MfeI C'AATT_G
Cuts at: 0 221 372
Size: 221 151
MslI CAynn'nnrTG
Cuts at: 0 199 372
Size: 199 173
PpuMI rG'GwC_Cy
Cuts at: 0 276 372
Size: 276 96
StyI C'CwwG_G
Cuts at: 0 280 372
Size: 280 92
TatI w'GTAC_w
Cuts at: 0 240 372
Size: 240 132
Tth111I GACn'n_nGTC
Cuts at: 0 233 372
Size: 233 139
Enzymes that do cut:
BbsI BglII BseRI BsgI BsrDI BstBI BstYI BtgZI
EcoO109I EcoRV FalI MfeI MslI PpuMI StyI TatI
Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI XbaI XcmI XhoI
XmaI XmnI ZraI