(Linear) (Six-base) MAPSORT of: KIBB7907.seq Check: 9449 from: 1 to: 393
KIBB7907.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 393
Size: 328 65
AflIII A'CryG_T
Cuts at: 0 221 393
Size: 221 172
ApoI r'AATT_y
Cuts at: 0 67 283 393
Size: 67 216 110
Fragments arranged by size:
216 110 67
BsmI GAATG_Cn'
Cuts at: 0 273 393
Size: 273 120
BsrDI GCAATG_nn'
Cuts at: 0 82 393
Size: 82 311
EaeI y'GGCC_r
Cuts at: 0 2 393
Size: 2 391
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 393
Size: 328 65
HindIII A'AGCT_T
Cuts at: 0 137 393
Size: 137 256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 44 393
Size: 38 6 349
Fragments arranged by size:
349 38 6
MspA1I CmG'CkG
Cuts at: 0 7 393
Size: 7 386
NspI r_CATG'y
Cuts at: 0 225 393
Size: 225 168
PciI A'CATG_T
Cuts at: 0 221 393
Size: 221 172
PfoI T'CCnGG_A
Cuts at: 0 325 393
Size: 325 68
StyI C'CwwG_G
Cuts at: 0 353 393
Size: 353 40
Enzymes that do cut:
AcuI AflIII ApoI BsmI BsrDI EaeI Eco57MI HindIII
MmeI MspA1I NspI PciI PfoI StyI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI