Restriction map for
pF1KB7916
(Linear) (Six-base) MAPSORT of: KIBB7916.seq Check: 128 from: 1 to: 1035
KIBB7916.orf
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AatII G_ACGT'C
Cuts at: 0 944 1035
Size: 944 91
AccI GT'mk_AC
Cuts at: 0 770 1035
Size: 770 265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 1035
Size: 338 697
AfeI AGC'GCT
Cuts at: 0 834 1035
Size: 834 201
AgeI A'CCGG_T
Cuts at: 0 735 1035
Size: 735 300
ApaI G_GGCC'C
Cuts at: 0 326 1035
Size: 326 709
AvaI C'yCGr_G
Cuts at: 0 191 428 1035
Size: 191 237 607
Fragments arranged by size:
607 237 191
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 230 263 1035
Size: 230 33 772
Fragments arranged by size:
772 230 33
BamHI G'GATC_C
Cuts at: 0 164 1035
Size: 164 871
BanI G'GyrC_C
Cuts at: 0 1013 1035
Size: 1013 22
BanII G_rGCy'C
Cuts at: 0 191 326 474 752 872 1035
Size: 191 135 148 278 120 163
Fragments arranged by size:
278 191 163 148 135 120
BbsI GAAGACnn'nnnn_
Cuts at: 0 227 1035
Size: 227 808
BbvCI CC'TCA_GC
Cuts at: 0 544 1035
Size: 544 491
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 163 197 1035
Size: 163 34 838
Fragments arranged by size:
838 163 34
BglI GCCn_nnn'nGGC
Cuts at: 0 511 991 1022 1035
Size: 511 480 31 13
Fragments arranged by size:
511 480 31 13
Bme1580I G_kGCm'C
Cuts at: 0 326 1035
Size: 326 709
BmgBI CAC'GTC
Cuts at: 0 805 1035
Size: 805 230
BmrI ACTGGGnnnn_n'
Cuts at: 0 820 1035
Size: 820 215
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 734 1035
Size: 734 301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 1035
Size: 225 810
Bpu10I CC'TnA_GC
Cuts at: 0 544 1035
Size: 544 491
BsaI GGTCTCn'nnnn_
Cuts at: 0 290 1035
Size: 290 745
BsaHI Gr'CG_yC
Cuts at: 0 941 1035
Size: 941 94
BsaWI w'CCGG_w
Cuts at: 0 735 1035
Size: 735 300
BseYI C'CCAG_C
Cuts at: 0 137 560 1035
Size: 137 423 475
Fragments arranged by size:
475 423 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 896 1035
Size: 69 827 139
Fragments arranged by size:
827 139 69
BsmI GAATG_Cn'
Cuts at: 0 1025 1035
Size: 1025 10
Bsp1286I G_dGCh'C
Cuts at: 0 191 326 474 752 872 1035
Size: 191 135 148 278 120 163
Fragments arranged by size:
278 191 163 148 135 120
BsrFI r'CCGG_y
Cuts at: 0 735 1035
Size: 735 300
BstYI r'GATC_y
Cuts at: 0 164 1035
Size: 164 871
Bsu36I CC'TnA_GG
Cuts at: 0 383 1035
Size: 383 652
BtgI C'CryG_G
Cuts at: 0 409 839 1035
Size: 409 430 196
Fragments arranged by size:
430 409 196
DraIII CAC_nnn'GTG
Cuts at: 0 687 1035
Size: 687 348
DrdI GACnn_nn'nnGTC
Cuts at: 0 208 809 1035
Size: 208 601 226
Fragments arranged by size:
601 226 208
EaeI y'GGCC_r
Cuts at: 0 807 1035
Size: 807 228
EarI CTCTTCn'nnn_
Cuts at: 0 899 1035
Size: 899 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 709 1035
Size: 709 326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 338 1035
Size: 225 113 697
Fragments arranged by size:
697 225 113
EcoNI CCTnn'n_nnAGG
Cuts at: 0 898 1035
Size: 898 137
EcoO109I rG'GnC_Cy
Cuts at: 0 322 323 1035
Size: 322 1 712
Fragments arranged by size:
712 322 1
FspI TGC'GCA
Cuts at: 0 25 1035
Size: 25 1010
HaeII r_GCGC'y
Cuts at: 0 836 1035
Size: 836 199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 282 387 1035
Size: 39 243 105 648
Fragments arranged by size:
648 243 105 39
MspA1I CmG'CkG
Cuts at: 0 480 1035
Size: 480 555
NcoI C'CATG_G
Cuts at: 0 839 1035
Size: 839 196
NspI r_CATG'y
Cuts at: 0 184 1035
Size: 184 851
PasI CC'CwG_GG
Cuts at: 0 465 1009 1035
Size: 465 544 26
Fragments arranged by size:
544 465 26
PspOMI G'GGCC_C
Cuts at: 0 322 1035
Size: 322 713
PstI C_TGCA'G
Cuts at: 0 855 973 1035
Size: 855 118 62
Fragments arranged by size:
855 118 62
SfcI C'TryA_G
Cuts at: 0 851 969 1035
Size: 851 118 66
Fragments arranged by size:
851 118 66
SmaI CCC'GGG
Cuts at: 0 193 430 1035
Size: 193 237 605
Fragments arranged by size:
605 237 193
SphI G_CATG'C
Cuts at: 0 184 1035
Size: 184 851
StyI C'CwwG_G
Cuts at: 0 234 509 839 1035
Size: 234 275 330 196
Fragments arranged by size:
330 275 234 196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 145 1035
Size: 145 890
XmaI C'CCGG_G
Cuts at: 0 191 428 1035
Size: 191 237 607
Fragments arranged by size:
607 237 191
ZraI GAC'GTC
Cuts at: 0 942 1035
Size: 942 93
Enzymes that do cut:
AatII AccI AcuI AfeI AgeI ApaI AvaI BaeI
BamHI BanI BanII BbsI BbvCI BcgI BglI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI
BseYI BsgI BsmI Bsp1286I BsrFI BstYI Bsu36I BtgI
DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FspI HaeII MmeI MspA1I NcoI NspI PasI PspOMI
PstI SfcI SmaI SphI StyI XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AhdI AleI AlfI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BbeI BciVI BclI BfrBI BglII BlpI BmtI BpuEI
BsaAI BsaBI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI
CspCI DraI EagI EcoICRI EcoRI EcoRV FalI FseI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmnI