Restriction map for
pF1KB7918
(Linear) (Six-base) MAPSORT of: KIBB7918.seq Check: 8136 from: 1 to: 1038
KIBB7918.orf
With 182 enzymes: SgfI *
November 10, 2008 10:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 601 982 1038
Size: 432 169 381 56
Fragments arranged by size:
432 381 169 56
AhdI GACnn_n'nnGTC
Cuts at: 0 257 1038
Size: 257 781
AlwNI CAG_nnn'CTG
Cuts at: 0 409 1038
Size: 409 629
ApaI G_GGCC'C
Cuts at: 0 382 1038
Size: 382 656
AvaI C'yCGr_G
Cuts at: 0 15 76 375 692 1038
Size: 15 61 299 317 346
Fragments arranged by size:
346 317 299 61 15
BamHI G'GATC_C
Cuts at: 0 24 833 1038
Size: 24 809 205
Fragments arranged by size:
809 205 24
BanI G'GyrC_C
Cuts at: 0 289 1027 1038
Size: 289 738 11
Fragments arranged by size:
738 289 11
BanII G_rGCy'C
Cuts at: 0 382 714 938 990 1038
Size: 382 332 224 52 48
Fragments arranged by size:
382 332 224 52 48
BbeI G_GCGC'C
Cuts at: 0 293 1038
Size: 293 745
Bme1580I G_kGCm'C
Cuts at: 0 382 401 1030 1038
Size: 382 19 629 8
Fragments arranged by size:
629 382 19 8
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 1030 1038
Size: 596 434 8
Fragments arranged by size:
596 434 8
BsaI GGTCTCn'nnnn_
Cuts at: 0 889 1038
Size: 889 149
BsaHI Gr'CG_yC
Cuts at: 0 92 290 601 1038
Size: 92 198 311 437
Fragments arranged by size:
437 311 198 92
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 212 242 1038
Size: 212 30 796
Fragments arranged by size:
796 212 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 145 717 970 1038
Size: 100 45 572 253 68
Fragments arranged by size:
572 253 100 68 45
BseYI C'CCAG_C
Cuts at: 0 368 510 713 989 1038
Size: 368 142 203 276 49
Fragments arranged by size:
368 276 203 142 49
BsiEI CG_ry'CG
Cuts at: 0 473 641 1038
Size: 473 168 397
Fragments arranged by size:
473 397 168
BsiHKAI G_wGCw'C
Cuts at: 0 938 1038
Size: 938 100
Bsp1286I G_dGCh'C
Cuts at: 0 382 401 714 938 990 1030 1038
Size: 382 19 313 224 52 40 8
Fragments arranged by size:
382 313 224 52 40 19 8
BspHI T'CATG_A
Cuts at: 0 593 1038
Size: 593 445
BsrBI CCG'CTC
Cuts at: 0 127 732 915 1038
Size: 127 605 183 123
Fragments arranged by size:
605 183 127 123
BsrDI GCAATG_nn'
Cuts at: 0 459 1038
Size: 459 579
BstAPI GCAn_nnn'nTGC
Cuts at: 0 499 1038
Size: 499 539
BstBI TT'CG_AA
Cuts at: 0 775 1038
Size: 775 263
BstYI r'GATC_y
Cuts at: 0 24 833 1038
Size: 24 809 205
Fragments arranged by size:
809 205 24
Bsu36I CC'TnA_GG
Cuts at: 0 940 1038
Size: 940 98
EaeI y'GGCC_r
Cuts at: 0 38 146 270 470 1038
Size: 38 108 124 200 568
Fragments arranged by size:
568 200 124 108 38
EagI C'GGCC_G
Cuts at: 0 470 1038
Size: 470 568
EarI CTCTTCn'nnn_
Cuts at: 0 702 1038
Size: 702 336
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 97 753 1038
Size: 97 656 285
Fragments arranged by size:
656 285 97
EcoICRI GAG'CTC
Cuts at: 0 936 1038
Size: 936 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 596 601 982 1030 1038
Size: 432 164 5 381 48 8
Fragments arranged by size:
432 381 164 48 8 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 841 1038
Size: 841 197
EcoO109I rG'GnC_Cy
Cuts at: 0 73 379 406 1038
Size: 73 306 27 632
Fragments arranged by size:
632 306 73 27
HaeII r_GCGC'y
Cuts at: 0 293 375 418 1038
Size: 293 82 43 620
Fragments arranged by size:
620 293 82 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 243 275 426 458 1038
Size: 243 32 151 32 580
Fragments arranged by size:
580 243 151 32 32
KasI G'GCGC_C
Cuts at: 0 289 1038
Size: 289 749
MscI TGG'CCA
Cuts at: 0 148 1038
Size: 148 890
MspA1I CmG'CkG
Cuts at: 0 31 46 142 264 286 313 388 426
Size: 31 15 96 122 22 27 75 38
Cuts at: 426 485 993 1038
Size: 59 508 45
Fragments arranged by size:
508 122 96 75 59 45 38 31
27 22 15
NarI GG'CG_CC
Cuts at: 0 290 1038
Size: 290 748
NspI r_CATG'y
Cuts at: 0 9 891 1038
Size: 9 882 147
Fragments arranged by size:
882 147 9
PfoI T'CCnGG_A
Cuts at: 0 860 1038
Size: 860 178
PpuMI rG'GwC_Cy
Cuts at: 0 73 406 1038
Size: 73 333 632
Fragments arranged by size:
632 333 73
PshAI GACnn'nnGTC
Cuts at: 0 17 1038
Size: 17 1021
PspOMI G'GGCC_C
Cuts at: 0 378 1038
Size: 378 660
PstI C_TGCA'G
Cuts at: 0 104 414 923 998 1038
Size: 104 310 509 75 40
Fragments arranged by size:
509 310 104 75 40
PvuI CG_AT'CG
Cuts at: 0 641 1038
Size: 641 397
PvuII CAG'CTG
Cuts at: 0 264 286 993 1038
Size: 264 22 707 45
Fragments arranged by size:
707 264 45 22
SacI G_AGCT'C
Cuts at: 0 938 1038
Size: 938 100
SanDI GG'GwC_CC
Cuts at: 0 73 1038
Size: 73 965
SapI GCTCTTCn'nnn_
Cuts at: 0 702 1038
Size: 702 336
SfcI C'TryA_G
Cuts at: 0 100 410 919 994 1038
Size: 100 310 509 75 44
Fragments arranged by size:
509 310 100 75 44
SfoI GGC'GCC
Cuts at: 0 291 1038
Size: 291 747
SmaI CCC'GGG
Cuts at: 0 17 377 1038
Size: 17 360 661
Fragments arranged by size:
661 360 17
SphI G_CATG'C
Cuts at: 0 891 1038
Size: 891 147
SrfI GCCC'GGGC
Cuts at: 0 377 1038
Size: 377 661
StuI AGG'CCT
Cuts at: 0 847 1038
Size: 847 191
StyI C'CwwG_G
Cuts at: 0 842 1038
Size: 842 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 340 1038
Size: 340 698
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 212 244 1038
Size: 212 32 794
Fragments arranged by size:
794 212 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 253 1038
Size: 253 785
XmaI C'CCGG_G
Cuts at: 0 15 375 1038
Size: 15 360 663
Fragments arranged by size:
663 360 15
Enzymes that do cut:
AcuI AhdI AlwNI ApaI AvaI BamHI BanI BanII
BbeI Bme1580I BpmI BsaI BsaHI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspHI BsrBI BsrDI BstAPI BstBI
BstYI Bsu36I EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MscI MspA1I NarI
NspI PfoI PpuMI PshAI PspOMI PstI PvuI PvuII
SacI SanDI SapI SfcI SfoI SmaI SphI SrfI
StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaWI BsgI BsiWI BsmI
BsmBI BspEI BspMI BsrFI BsrGI BssHII BssSI BstEII
BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PsiI
PspXI PsrI RsrII SacII SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SspI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI