(Linear) (Six-base) MAPSORT of: KIBB7919.seq  Check: 2227  from: 1  to: 540



KIBB7919.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

BanI G'GyrC_C

Cuts at:      0     139     540 
   Size:        139     401
 
BanII G_rGCy'C

Cuts at:      0      19     540 
   Size:         19     521
 
BglI GCCn_nnn'nGGC

Cuts at:      0      33     540 
   Size:         33     507
 
BglII A'GATC_T

Cuts at:      0     363     540 
   Size:        363     177
 
BlpI GC'TnA_GC

Cuts at:      0     338     540 
   Size:        338     202
 
Bme1580I G_kGCm'C

Cuts at:      0     142     540 
   Size:        142     398
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      75     165     540 
   Size:         75      90     375

  Fragments arranged by size: 

                 375      90      75
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     353     540 
   Size:        353     187
 
BsaWI w'CCGG_w

Cuts at:      0      89     540 
   Size:         89     451
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     521     540 
   Size:        521      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     540 
   Size:         66     474
 
BsiHKAI G_wGCw'C

Cuts at:      0     339     540 
   Size:        339     201
 
Bsp1286I G_dGCh'C

Cuts at:      0      19     142     339     540 
   Size:         19     123     197     201

  Fragments arranged by size: 

                 201     197     123      19
 
BspEI T'CCGG_A

Cuts at:      0      89     540 
   Size:         89     451
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     177     540 
   Size:        177     363
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     122     204     421     540 
   Size:        122      82     217     119

  Fragments arranged by size: 

                 217     122     119      82
 
BstYI r'GATC_y

Cuts at:      0     363     540 
   Size:        363     177
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     140     540 
   Size:        140     400
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      76     540 
   Size:         76     464
 
EarI CTCTTCn'nnn_

Cuts at:      0       7     540 
   Size:          7     533
 
HaeII r_GCGC'y

Cuts at:      0     101     540 
   Size:        101     439
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     339     540 
   Size:        339     201
 
PstI C_TGCA'G

Cuts at:      0     435     540 
   Size:        435     105
 
SapI GCTCTTCn'nnn_

Cuts at:      0       7     540 
   Size:          7     533
 
SfcI C'TryA_G

Cuts at:      0      72     431     540 
   Size:         72     359     109

  Fragments arranged by size: 

                 359     109      72
 
StuI AGG'CCT

Cuts at:      0     106     540 
   Size:        106     434
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     516     540 
   Size:        516      24
 

 Enzymes that do cut:

     BanI    BanII     BglI    BglII     BlpI Bme1580I     BmrI     BplI
    BsaWI    BseRI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI   BstAPI
    BstYI    BtgZI     DrdI     EarI    HaeII     MmeI     PstI     SapI
     SfcI     StuI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BmgBI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI